Restriction map for
pF1KB3682
(Circular) (Six-base) MAPSORT of: pF1KB3682.seq Check: 7054 from: 1 to: 5369
pF1KB3682 5369 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1340 1826 1340
Size: 486 4883
AccI GT'mk_AC
Cuts at: 2407 2407
Size: 5369
Acc65I G'GTAC_C
Cuts at: 2385 2385
Size: 5369
AclI AA'CG_TT
Cuts at: 5209 5209
Size: 5369
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 118 367 460 1014 1415 2125 2248 2264 3194
Size: 249 93 554 401 710 123 16 930
Cuts at: 3194 3626 3979 4641 118
Size: 432 353 662 846
Fragments arranged by size:
930 846 710 662 554 432 401 353
249 123 93 16
AflIII A'CryG_T
Cuts at: 1673 2721 4521 4802 1673
Size: 1048 1800 281 2240
Fragments arranged by size:
2240 1800 1048 281
AhdI GACnn_n'nnGTC
Cuts at: 294 2313 294
Size: 2019 3350
AleI CACnn'nnGTG
Cuts at: 1276 1960 1276
Size: 684 4685
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 658 658
Size: 5369
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1979 2011 1979
Size: 32 5337
AlwNI CAG_nnn'CTG
Cuts at: 213 2674 4112 213
Size: 2461 1438 1470
Fragments arranged by size:
2461 1470 1438
ApaLI G'TGCA_C
Cuts at: 1589 4207 1589
Size: 2618 2751
ApoI r'AATT_y
Cuts at: 151 455 1321 1764 2338 2373 4722 4978 151
Size: 304 866 443 574 35 2349 256 542
Fragments arranged by size:
2349 866 574 542 443 304 256 35
AseI AT'TA_AT
Cuts at: 20 809 20
Size: 789 4580
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5369
AvaI C'yCGr_G
Cuts at: 1456 1506 1934 2389 1456
Size: 50 428 455 4436
Fragments arranged by size:
4436 455 428 50
BamHI G'GATC_C
Cuts at: 2394 2394
Size: 5369
BanI G'GyrC_C
Cuts at: 1328 1360 2385 2799 3047 3082 1328
Size: 32 1025 414 248 35 3615
Fragments arranged by size:
3615 1025 414 248 35 32
BanII G_rGCy'C
Cuts at: 1060 1602 1933 2332 2383 3413 1060
Size: 542 331 399 51 1030 3016
Fragments arranged by size:
3016 1030 542 399 331 51
BbeI G_GCGC'C
Cuts at: 2803 3051 2803
Size: 248 5121
BbsI GAAGACnn'nnnn_
Cuts at: 407 1126 407
Size: 719 4650
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 913 947 1452 1486 913
Size: 34 505 34 4796
Fragments arranged by size:
4796 505 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2126 3267 4323 5369 2126
Size: 1141 1056 1046 2126
Fragments arranged by size:
2126 1141 1056 1046
BclI T'GATC_A
Cuts at: 309 422 684 2889 309
Size: 113 262 2205 2789
Fragments arranged by size:
2789 2205 262 113
BglII A'GATC_T
Cuts at: 2884 4921 2884
Size: 2037 3332
BlpI GC'TnA_GC
Cuts at: 1601 2481 1601
Size: 880 4489
Bme1580I G_kGCm'C
Cuts at: 1267 1593 1881 2994 3087 4211 1267
Size: 326 288 1113 93 1124 2425
Fragments arranged by size:
2425 1124 1113 326 288 93
BmrI ACTGGGnnnn_n'
Cuts at: 249 2581 2662 2997 5141 249
Size: 2332 81 335 2144 477
Fragments arranged by size:
2332 2144 477 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1422 1482 3736 1422
Size: 60 2254 3055
Fragments arranged by size:
3055 2254 60
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 178 2142 178
Size: 1964 3405
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 694 2545 3424 4138 4436 694
Size: 1851 879 714 298 1627
Fragments arranged by size:
1851 1627 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 655 1657 1723 5073 655
Size: 1002 66 3350 951
Fragments arranged by size:
3350 1002 951 66
BsaAI yAC'GTr
Cuts at: 2722 2722
Size: 5369
BsaBI GATnn'nnATC
Cuts at: 6 2883 4920 6
Size: 2877 2037 455
Fragments arranged by size:
2877 2037 455
BsaHI Gr'CG_yC
Cuts at: 2800 3048 3750 5246 2800
Size: 248 702 1496 2923
Fragments arranged by size:
2923 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2558 3079 3757 4168 4315 4589 2558
Size: 521 678 411 147 274 3338
Fragments arranged by size:
3338 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1805 1835 1805
Size: 30 5339
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1306 2314 1306
Size: 1008 4361
BseYI C'CCAG_C
Cuts at: 2794 3552 4217 4625 2794
Size: 758 665 408 3538
Fragments arranged by size:
3538 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 2911 2957 4187 104
Size: 2807 46 1230 1286
Fragments arranged by size:
2807 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 248 511 1060 1593 1933 2383 3161 3351 4211
Size: 263 549 533 340 450 778 190 860
Cuts at: 4211 248
Size: 1406
Fragments arranged by size:
1406 860 778 549 533 450 340 263
190
BsmI GAATG_Cn'
Cuts at: 804 804
Size: 5369
BsmBI CGTCTCn'nnnn_
Cuts at: 1453 1453
Size: 5369
Bsp1286I G_dGCh'C
Cuts at: 248 511 1060 1267 1593 1602 1881 1933 2332
Size: 263 549 207 326 9 279 52 399
Cuts at: 2332 2383 2994 3087 3161 3351 3413 4211 248
Size: 51 611 93 74 190 62 798 1406
Fragments arranged by size:
1406 798 611 549 399 326 279 263
207 190 93 74 62 52 51 9
BspHI T'CATG_A
Cuts at: 285 3801 285
Size: 3516 1853
BspMI ACCTGCnnnn'nnnn_
Cuts at: 153 1340 1826 2288 2419 2633 2935 3316 153
Size: 1187 486 462 131 214 302 381 2206
Fragments arranged by size:
2206 1187 486 462 381 302 214 131
BsrBI CCG'CTC
Cuts at: 50 1511 1968 3661 3715 5199 5364 50
Size: 1461 457 1693 54 1484 165 55
Fragments arranged by size:
1693 1484 1461 457 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3281 3281
Size: 5369
BsrFI r'CCGG_y
Cuts at: 3367 3548 3367
Size: 181 5188
BssSI C'ACGA_G
Cuts at: 658 3640 4348 658
Size: 2982 708 1679
Fragments arranged by size:
2982 1679 708
BstAPI GCAn_nnn'nTGC
Cuts at: 2746 2746
Size: 5369
BstBI TT'CG_AA
Cuts at: 3730 3730
Size: 5369
BstXI CCAn_nnnn'nTGG
Cuts at: 672 2143 672
Size: 1471 3898
BstYI r'GATC_y
Cuts at: 1 2394 2861 2884 3219 3465 3771 3783 3869
Size: 2393 467 23 335 246 306 12 86
Cuts at: 3869 4921 1
Size: 1052 449
Fragments arranged by size:
2393 1052 467 449 335 306 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 2297 2297
Size: 5369
BtgI C'CryG_G
Cuts at: 194 1726 194
Size: 1532 3837
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3319 3500 3663 3319
Size: 181 163 5025
Fragments arranged by size:
5025 181 163
BtsI GCAGTG_nn'
Cuts at: 2612 2612
Size: 5369
DraI TTT'AAA
Cuts at: 2368 2368
Size: 5369
DraIII CAC_nnn'GTG
Cuts at: 1588 1588
Size: 5369
DrdI GACnn_nn'nnGTC
Cuts at: 547 3075 4419 547
Size: 2528 1344 1497
Fragments arranged by size:
2528 1497 1344
EaeI y'GGCC_r
Cuts at: 2954 3128 3519 3546 4891 2954
Size: 174 391 27 1345 3432
Fragments arranged by size:
3432 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2954 2954
Size: 5369
EarI CTCTTCn'nnn_
Cuts at: 1391 3392 3602 1391
Size: 2001 210 3158
Fragments arranged by size:
3158 2001 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1564 3577 4319 4465 5179 1564
Size: 2013 742 146 714 1754
Fragments arranged by size:
2013 1754 742 714 146
EcoICRI GAG'CTC
Cuts at: 1058 1931 2381 1058
Size: 873 450 4046
Fragments arranged by size:
4046 873 450
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 118 178 367 460 1014 1415 2125 2142 2248
Size: 60 189 93 554 401 710 17 106
Cuts at: 2248 2264 3194 3626 3979 4641 118
Size: 16 930 432 353 662 846
Fragments arranged by size:
930 846 710 662 554 432 401 353
189 106 93 60 17 16
EcoNI CCTnn'n_nnAGG
Cuts at: 160 1885 160
Size: 1725 3644
EcoO109I rG'GnC_Cy
Cuts at: 1378 1904 2508 1378
Size: 526 604 4239
Fragments arranged by size:
4239 604 526
EcoRI G'AATT_C
Cuts at: 2338 2373 2338
Size: 35 5334
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3388 3388
Size: 5369
FspI TGC'GCA
Cuts at: 3150 3150
Size: 5369
HaeII r_GCGC'y
Cuts at: 851 2803 3051 4281 5052 851
Size: 1952 248 1230 771 1168
Fragments arranged by size:
1952 1230 1168 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 280 312 465 497 1452 1484 2299 2331 3211
Size: 32 153 32 955 32 815 32 880
Cuts at: 3211 3241 3243 3273 280
Size: 30 2 30 2376
Fragments arranged by size:
2376 955 880 815 153 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 2408 2408
Size: 5369
HindIII A'AGCT_T
Cuts at: 1687 1687
Size: 5369
KasI G'GCGC_C
Cuts at: 2799 3047 2799
Size: 248 5121
KpnI G_GTAC'C
Cuts at: 2389 2389
Size: 5369
MfeI C'AATT_G
Cuts at: 1336 1426 1336
Size: 90 5279
MluI A'CGCG_T
Cuts at: 4802 4802
Size: 5369
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 932 1514 4128 4312 932
Size: 582 2614 184 1989
Fragments arranged by size:
2614 1989 582 184
MscI TGG'CCA
Cuts at: 3130 3130
Size: 5369
MslI CAynn'nnrTG
Cuts at: 112 514 1276 1543 1956 1960 3358 3485 112
Size: 402 762 267 413 4 1398 127 1996
Fragments arranged by size:
1996 1398 762 413 402 267 127 4
MspA1I CmG'CkG
Cuts at: 303 434 702 888 1110 1668 2480 2794 3154
Size: 131 268 186 222 558 812 314 360
Cuts at: 3154 3938 4183 303
Size: 784 245 1489
Fragments arranged by size:
1489 812 784 558 360 314 268 245
222 186 131
NaeI GCC'GGC
Cuts at: 3550 3550
Size: 5369
NarI GG'CG_CC
Cuts at: 2800 3048 2800
Size: 248 5121
NcoI C'CATG_G
Cuts at: 194 194
Size: 5369
NgoMIV G'CCGG_C
Cuts at: 3548 3548
Size: 5369
NspI r_CATG'y
Cuts at: 1089 1542 1677 2422 2922 4525 1089
Size: 453 135 745 500 1603 1933
Fragments arranged by size:
1933 1603 745 500 453 135
PciI A'CATG_T
Cuts at: 1673 4521 1673
Size: 2848 2521
PflMI CCAn_nnn'nTGG
Cuts at: 1529 1529
Size: 5369
PfoI T'CCnGG_A
Cuts at: 155 155
Size: 5369
PmeI GTTT'AAAC
Cuts at: 2368 2368
Size: 5369
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3815 3847 3815
Size: 32 5337
PpuMI rG'GwC_Cy
Cuts at: 1378 1904 1378
Size: 526 4843
PshAI GACnn'nnGTC
Cuts at: 1126 1126
Size: 5369
PsiI TTA'TAA
Cuts at: 1181 1181
Size: 5369
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 689 721 689
Size: 32 5337
PstI C_TGCA'G
Cuts at: 893 926 2416 3101 893
Size: 33 1490 685 3161
Fragments arranged by size:
3161 1490 685 33
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5369
PvuII CAG'CTG
Cuts at: 303 434 702 888 1668 2794 3154 303
Size: 131 268 186 780 1126 360 2518
Fragments arranged by size:
2518 1126 780 360 268 186 131
RsrII CG'GwC_CG
Cuts at: 3564 3564
Size: 5369
SacI G_AGCT'C
Cuts at: 1060 1933 2383 1060
Size: 873 450 4046
Fragments arranged by size:
4046 873 450
SalI G'TCGA_C
Cuts at: 2406 2406
Size: 5369
SapI GCTCTTCn'nnn_
Cuts at: 1391 3392 3602 1391
Size: 2001 210 3158
Fragments arranged by size:
3158 2001 210
SbfI CC_TGCA'GG
Cuts at: 2416 2416
Size: 5369
ScaI AGT'ACT
Cuts at: 4886 4886
Size: 5369
SfcI C'TryA_G
Cuts at: 33 349 889 905 922 2333 2412 3097 4065
Size: 316 540 16 17 1411 79 685 968
Cuts at: 4065 4256 33
Size: 191 1146
Fragments arranged by size:
1411 1146 968 685 540 316 191 79
17 16
SfoI GGC'GCC
Cuts at: 2801 3049 2801
Size: 248 5121
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5369
SmaI CCC'GGG
Cuts at: 1936 2391 1936
Size: 455 4914
SmlI C'TyrA_G
Cuts at: 709 1456 2524 3439 4153 4415 709
Size: 747 1068 915 714 262 1663
Fragments arranged by size:
1663 1068 915 747 714 262
SpeI A'CTAG_T
Cuts at: 67 819 67
Size: 752 4617
SphI G_CATG'C
Cuts at: 1089 2422 2922 1089
Size: 1333 500 3536
Fragments arranged by size:
3536 1333 500
SspI AAT'ATT
Cuts at: 501 2088 2124 501
Size: 1587 36 3746
Fragments arranged by size:
3746 1587 36
StyI C'CwwG_G
Cuts at: 194 2503 2857 194
Size: 2309 354 2706
Fragments arranged by size:
2706 2309 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1829 1950 2952 3085 3544 3753 1829
Size: 121 1002 133 459 209 3445
Fragments arranged by size:
3445 1002 459 209 133 121
TatI w'GTAC_w
Cuts at: 4884 4884
Size: 5369
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4886 4918 4886
Size: 32 5337
Tth111I GACn'n_nGTC
Cuts at: 3166 3166
Size: 5369
XbaI T'CTAG_A
Cuts at: 2257 2400 2257
Size: 143 5226
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 876 1308 1592 2864 876
Size: 432 284 1272 3381
Fragments arranged by size:
3381 1272 432 284
XhoI C'TCGA_G
Cuts at: 1456 1456
Size: 5369
XmaI C'CCGG_G
Cuts at: 1934 2389 1934
Size: 455 4914
XmnI GAAnn'nnTTC
Cuts at: 340 752 898 1400 340
Size: 412 146 502 4309
Fragments arranged by size:
4309 502 412 146
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AhdI AleI
AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PsrI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AgeI ApaI AscI AvrII BaeI
BbvCI BfrBI BglI BmgBI BmtI Bpu10I BsgI BsiWI
BspEI BsrGI BssHII BstEII BstZ17I ClaI CspCI EcoRV
FseI FspAI HpaI NdeI NheI NotI NruI NsiI
PacI PasI PmlI PspOMI PspXI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI StuI SwaI ZraI