Restriction map for
pF1KB3671
(Circular) (Six-base) MAPSORT of: pF1KB3671.seq Check: 7433 from: 1 to: 4241
pF1KB3671 4241 bp
With 182 enzymes: SgfI *
April 24, 2009 13:02 ..
AccI GT'mk_AC
Cuts at: 1279 1279
Size: 4241
Acc65I G'GTAC_C
Cuts at: 1257 1257
Size: 4241
AclI AA'CG_TT
Cuts at: 415 4081 415
Size: 3666 575
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 353 475 2066 2498 3245 3513 353
Size: 122 1591 432 747 268 1081
Fragments arranged by size:
1591 1081 747 432 268 122
AflIII A'CryG_T
Cuts at: 188 1593 2697 3674 188
Size: 1405 1104 977 755
Fragments arranged by size:
1405 1104 977 755
AgeI A'CCGG_T
Cuts at: 2629 2629
Size: 4241
AleI CACnn'nnGTG
Cuts at: 334 334
Size: 4241
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1146 1146
Size: 4241
AlwNI CAG_nnn'CTG
Cuts at: 279 360 756 1154 1546 3113 279
Size: 81 396 398 392 1567 1407
Fragments arranged by size:
1567 1407 398 396 392 81
ApaLI G'TGCA_C
Cuts at: 1051 3011 1051
Size: 1960 2281
ApoI r'AATT_y
Cuts at: 919 1245 3594 3850 919
Size: 326 2349 256 1310
Fragments arranged by size:
2349 1310 326 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4241
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4241
AvaI C'yCGr_G
Cuts at: 1261 1261
Size: 4241
AvrII C'CTAG_G
Cuts at: 237 237
Size: 4241
BamHI G'GATC_C
Cuts at: 1266 1266
Size: 4241
BanI G'GyrC_C
Cuts at: 1084 1257 1671 1919 1954 1084
Size: 173 414 248 35 3371
Fragments arranged by size:
3371 414 248 173 35
BanII G_rGCy'C
Cuts at: 824 1255 2285 824
Size: 431 1030 2780
Fragments arranged by size:
2780 1030 431
BbeI G_GCGC'C
Cuts at: 1675 1923 1675
Size: 248 3993
BbsI GAAGACnn'nnnn_
Cuts at: 305 424 305
Size: 119 4122
BciVI GTATCCnnnnn_n'
Cuts at: 2139 2900 4241 2139
Size: 761 1341 2139
Fragments arranged by size:
2139 1341 761
BclI T'GATC_A
Cuts at: 1761 1761
Size: 4241
BglII A'GATC_T
Cuts at: 1756 3793 1756
Size: 2037 2204
BlpI GC'TnA_GC
Cuts at: 449 784 823 1353 449
Size: 335 39 530 3337
Fragments arranged by size:
3337 530 335 39
Bme1580I G_kGCm'C
Cuts at: 1055 1866 1959 3015 1055
Size: 811 93 1056 2281
Fragments arranged by size:
2281 1056 811 93
BmrI ACTGGGnnnn_n'
Cuts at: 1453 1534 1869 4013 1453
Size: 81 335 2144 1681
Fragments arranged by size:
2144 1681 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2608 2608
Size: 4241
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 778 778
Size: 4241
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1417 2296 2788 3086 1417
Size: 879 492 298 2572
Fragments arranged by size:
2572 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 519 1022 3945 519
Size: 503 2923 815
Fragments arranged by size:
2923 815 503
BsaAI yAC'GTr
Cuts at: 732 1594 732
Size: 862 3379
BsaBI GATnn'nnATC
Cuts at: 6 1755 3792 6
Size: 1749 2037 455
Fragments arranged by size:
2037 1749 455
BsaHI Gr'CG_yC
Cuts at: 1672 1920 2622 4118 1672
Size: 248 702 1496 1795
Fragments arranged by size:
1795 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1430 1951 2629 2903 3050 3461 1430
Size: 521 678 274 147 411 2210
Fragments arranged by size:
2210 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 700 730 700
Size: 30 4211
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 204 691 204
Size: 487 3754
BseYI C'CCAG_C
Cuts at: 1666 2424 3001 3497 1666
Size: 758 577 496 2410
Fragments arranged by size:
2410 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 712 712
Size: 4241
BsiEI CG_ry'CG
Cuts at: 104 1783 1829 3037 104
Size: 1679 46 1208 1308
Fragments arranged by size:
1679 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 824 1055 1255 2033 2223 3015 824
Size: 231 200 778 190 792 2050
Fragments arranged by size:
2050 792 778 231 200 190
BsmI GAATG_Cn'
Cuts at: 1205 1205
Size: 4241
Bsp1286I G_dGCh'C
Cuts at: 824 1055 1255 1866 1959 2033 2223 2285 3015
Size: 231 200 611 93 74 190 62 730
Cuts at: 3015 824
Size: 2050
Fragments arranged by size:
2050 730 611 231 200 190 93 74
62
BspEI T'CCGG_A
Cuts at: 3461 3461
Size: 4241
BspHI T'CATG_A
Cuts at: 3417 3417
Size: 4241
BspMI ACCTGCnnnn'nnnn_
Cuts at: 368 1211 1291 1505 1807 2188 368
Size: 843 80 214 302 381 2421
Fragments arranged by size:
2421 843 381 302 214 80
BsrBI CCG'CTC
Cuts at: 50 2533 2587 4071 4236 50
Size: 2483 54 1484 165 55
Fragments arranged by size:
2483 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1019 2153 1019
Size: 1134 3107
BsrFI r'CCGG_y
Cuts at: 2239 2420 2629 2239
Size: 181 209 3851
Fragments arranged by size:
3851 209 181
BssHII G'CGCG_C
Cuts at: 744 746 744
Size: 2 4239
BssSI C'ACGA_G
Cuts at: 2512 2870 2512
Size: 358 3883
BstAPI GCAn_nnn'nTGC
Cuts at: 1618 1618
Size: 4241
BstBI TT'CG_AA
Cuts at: 2602 2602
Size: 4241
BstYI r'GATC_y
Cuts at: 1 1266 1733 1756 2091 2337 3349 3435 3447
Size: 1265 467 23 335 246 1012 86 12
Cuts at: 3447 3793 1
Size: 346 449
Fragments arranged by size:
1265 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 227 107
Size: 120 4121
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1214 2191 2372 2535 1214
Size: 977 181 163 2920
Fragments arranged by size:
2920 977 181 163
BtsI GCAGTG_nn'
Cuts at: 1484 1484
Size: 4241
DraI TTT'AAA
Cuts at: 155 287 663 924 1240 155
Size: 132 376 261 316 3156
Fragments arranged by size:
3156 376 316 261 132
DraIII CAC_nnn'GTG
Cuts at: 189 189
Size: 4241
DrdI GACnn_nn'nnGTC
Cuts at: 1947 2805 1947
Size: 858 3383
EaeI y'GGCC_r
Cuts at: 224 371 1826 2000 2391 2418 3763 224
Size: 147 1455 174 391 27 1345 702
Fragments arranged by size:
1455 1345 702 391 174 147 27
EagI C'GGCC_G
Cuts at: 1826 1826
Size: 4241
EarI CTCTTCn'nnn_
Cuts at: 479 536 883 2264 2474 479
Size: 57 347 1381 210 2246
Fragments arranged by size:
2246 1381 347 210 57
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2449 2759 2905 4051 2449
Size: 310 146 1146 2639
Fragments arranged by size:
2639 1146 310 146
EcoICRI GAG'CTC
Cuts at: 822 1253 822
Size: 431 3810
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 353 475 778 2066 2498 3245 3513 353
Size: 122 303 1288 432 747 268 1081
Fragments arranged by size:
1288 1081 747 432 303 268 122
EcoNI CCTnn'n_nnAGG
Cuts at: 771 771
Size: 4241
EcoO109I rG'GnC_Cy
Cuts at: 1380 1380
Size: 4241
EcoRI G'AATT_C
Cuts at: 1245 1245
Size: 4241
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 152 863 928 1020 2260 152
Size: 711 65 92 1240 2133
Fragments arranged by size:
2133 1240 711 92 65
FspI TGC'GCA
Cuts at: 2022 2022
Size: 4241
HaeII r_GCGC'y
Cuts at: 1675 1923 2945 3924 1675
Size: 248 1022 979 1992
Fragments arranged by size:
1992 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 150 182 687 719 934 966 2083 2113 2115
Size: 32 505 32 215 32 1117 30 2
Cuts at: 2115 2145 150
Size: 30 2246
Fragments arranged by size:
2246 1117 505 215 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1280 1280
Size: 4241
KasI G'GCGC_C
Cuts at: 1671 1919 1671
Size: 248 3993
KpnI G_GTAC'C
Cuts at: 1261 1261
Size: 4241
MfeI C'AATT_G
Cuts at: 563 563
Size: 4241
MluI A'CGCG_T
Cuts at: 3674 3674
Size: 4241
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1011 2912 3096 1011
Size: 1901 184 2156
Fragments arranged by size:
2156 1901 184
MscI TGG'CCA
Cuts at: 226 2002 226
Size: 1776 2465
MslI CAynn'nnrTG
Cuts at: 334 2230 2357 334
Size: 1896 127 2218
Fragments arranged by size:
2218 1896 127
MspA1I CmG'CkG
Cuts at: 753 1021 1096 1352 1666 2026 3039 3284 753
Size: 268 75 256 314 360 1013 245 1710
Fragments arranged by size:
1710 1013 360 314 268 256 245 75
NaeI GCC'GGC
Cuts at: 2422 2422
Size: 4241
NarI GG'CG_CC
Cuts at: 1672 1920 1672
Size: 248 3993
NcoI C'CATG_G
Cuts at: 107 227 107
Size: 120 4121
NgoMIV G'CCGG_C
Cuts at: 2420 2420
Size: 4241
NspI r_CATG'y
Cuts at: 1294 1794 2701 1294
Size: 500 907 2834
Fragments arranged by size:
2834 907 500
PciI A'CATG_T
Cuts at: 2697 2697
Size: 4241
PfoI T'CCnGG_A
Cuts at: 490 490
Size: 4241
PmeI GTTT'AAAC
Cuts at: 1240 1240
Size: 4241
PmlI CAC'GTG
Cuts at: 732 732
Size: 4241
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3380 3412 3380
Size: 32 4209
PshAI GACnn'nnGTC
Cuts at: 1034 1034
Size: 4241
PstI C_TGCA'G
Cuts at: 483 1288 1973 483
Size: 805 685 2751
Fragments arranged by size:
2751 805 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4241
PvuII CAG'CTG
Cuts at: 753 1666 2026 753
Size: 913 360 2968
Fragments arranged by size:
2968 913 360
RsrII CG'GwC_CG
Cuts at: 2436 2436
Size: 4241
SacI G_AGCT'C
Cuts at: 824 1255 824
Size: 431 3810
SalI G'TCGA_C
Cuts at: 1278 1278
Size: 4241
SapI GCTCTTCn'nnn_
Cuts at: 2264 2474 2264
Size: 210 4031
SbfI CC_TGCA'GG
Cuts at: 1288 1288
Size: 4241
ScaI AGT'ACT
Cuts at: 894 3758 894
Size: 2864 1377
SfcI C'TryA_G
Cuts at: 33 479 1284 1969 2962 3153 33
Size: 446 805 685 993 191 1121
Fragments arranged by size:
1121 993 805 685 446 191
SfoI GGC'GCC
Cuts at: 1673 1921 1673
Size: 248 3993
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4241
SmaI CCC'GGG
Cuts at: 1263 1263
Size: 4241
SmlI C'TyrA_G
Cuts at: 1396 2311 2803 3065 1396
Size: 915 492 262 2572
Fragments arranged by size:
2572 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4241
SphI G_CATG'C
Cuts at: 1294 1794 1294
Size: 500 3741
StyI C'CwwG_G
Cuts at: 107 227 237 1375 1729 107
Size: 120 10 1138 354 2619
Fragments arranged by size:
2619 1138 354 120 10
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 541 1824 1957 2416 2625 541
Size: 1283 133 459 209 2157
Fragments arranged by size:
2157 1283 459 209 133
TatI w'GTAC_w
Cuts at: 892 3756 892
Size: 2864 1377
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3758 3790 3758
Size: 32 4209
Tth111I GACn'n_nGTC
Cuts at: 2038 2038
Size: 4241
XbaI T'CTAG_A
Cuts at: 1272 1272
Size: 4241
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1736 1736
Size: 4241
XmaI C'CCGG_G
Cuts at: 1261 1261
Size: 4241
XmnI GAAnn'nnTTC
Cuts at: 302 302
Size: 4241
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI
BanI BanII BbeI BbsI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PfoI
PmeI PmlI PpiI PshAI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI ApaI AscI
BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI Bpu10I
BsiWI BsmBI BsrGI BstEII BstXI BstZ17I Bsu36I ClaI
CspCI EcoRV FseI FspAI HindIII HpaI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PpuMI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI ZraI