Restriction map for
pF1KB3655
(Circular) (Six-base) MAPSORT of: pF1KB3655.seq Check: 8292 from: 1 to: 4796
pF1KB3655 4796 bp
With 182 enzymes: SgfI *
April 24, 2009 13:02 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1083 1083
Size: 4796
AccI GT'mk_AC
Cuts at: 1834 1834
Size: 4796
Acc65I G'GTAC_C
Cuts at: 1812 1812
Size: 4796
AclI AA'CG_TT
Cuts at: 4636 4636
Size: 4796
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 530 1085 2621 3053 3800 4068 530
Size: 555 1536 432 747 268 1258
Fragments arranged by size:
1536 1258 747 555 432 268
AflIII A'CryG_T
Cuts at: 911 2148 3252 4229 911
Size: 1237 1104 977 1478
Fragments arranged by size:
1478 1237 1104 977
AgeI A'CCGG_T
Cuts at: 3184 3184
Size: 4796
AleI CACnn'nnGTG
Cuts at: 445 445
Size: 4796
AlwNI CAG_nnn'CTG
Cuts at: 406 2101 3668 406
Size: 1695 1567 1534
Fragments arranged by size:
1695 1567 1534
ApaLI G'TGCA_C
Cuts at: 3566 3566
Size: 4796
ApoI r'AATT_y
Cuts at: 644 1800 4149 4405 644
Size: 1156 2349 256 1035
Fragments arranged by size:
2349 1156 1035 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4796
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4796
AvaI C'yCGr_G
Cuts at: 1337 1816 1337
Size: 479 4317
AvrII C'CTAG_G
Cuts at: 1611 1611
Size: 4796
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 674 707 674
Size: 33 4763
BamHI G'GATC_C
Cuts at: 363 425 1241 1821 363
Size: 62 816 580 3338
Fragments arranged by size:
3338 816 580 62
BanI G'GyrC_C
Cuts at: 1812 2226 2474 2509 1812
Size: 414 248 35 4099
Fragments arranged by size:
4099 414 248 35
BanII G_rGCy'C
Cuts at: 1264 1810 2840 1264
Size: 546 1030 3220
Fragments arranged by size:
3220 1030 546
BbeI G_GCGC'C
Cuts at: 2230 2478 2230
Size: 248 4548
BbsI GAAGACnn'nnnn_
Cuts at: 1383 1383
Size: 4796
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 955 989 955
Size: 34 4762
BciVI GTATCCnnnnn_n'
Cuts at: 539 2694 3455 4796 539
Size: 2155 761 1341 539
Fragments arranged by size:
2155 1341 761 539
BclI T'GATC_A
Cuts at: 2316 2316
Size: 4796
BglII A'GATC_T
Cuts at: 269 2311 4348 269
Size: 2042 2037 717
Fragments arranged by size:
2042 2037 717
BlpI GC'TnA_GC
Cuts at: 609 1908 609
Size: 1299 3497
Bme1580I G_kGCm'C
Cuts at: 2421 2514 3570 2421
Size: 93 1056 3647
Fragments arranged by size:
3647 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 490 503 1714 2008 2089 2424 4568 490
Size: 13 1211 294 81 335 2144 718
Fragments arranged by size:
2144 1211 718 335 294 81 13
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 288 309 3163 288
Size: 21 2854 1921
Fragments arranged by size:
2854 1921 21
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 173 416 445 173
Size: 243 29 4524
Fragments arranged by size:
4524 243 29
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 802 1972 2851 3343 3641 802
Size: 1170 879 492 298 1957
Fragments arranged by size:
1957 1170 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4500 4500
Size: 4796
BsaAI yAC'GTr
Cuts at: 2149 2149
Size: 4796
BsaBI GATnn'nnATC
Cuts at: 6 2310 4347 6
Size: 2304 2037 455
Fragments arranged by size:
2304 2037 455
BsaHI Gr'CG_yC
Cuts at: 2227 2475 3177 4673 2227
Size: 248 702 1496 2350
Fragments arranged by size:
2350 1496 702 248
BsaWI w'CCGG_w
Cuts at: 328 953 1985 2506 3184 3458 3605 4016 328
Size: 625 1032 521 678 274 147 411 1108
Fragments arranged by size:
1108 1032 678 625 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 253 298 301 253
Size: 45 3 4748
Fragments arranged by size:
4748 45 3
BseYI C'CCAG_C
Cuts at: 114 799 1194 2221 2979 3556 4052 114
Size: 685 395 1027 758 577 496 858
Fragments arranged by size:
1027 858 758 685 577 496 395
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1202 1202
Size: 4796
BsiEI CG_ry'CG
Cuts at: 104 2338 2384 3592 104
Size: 2234 46 1208 1308
Fragments arranged by size:
2234 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1264 1810 2588 2778 3570 1264
Size: 546 778 190 792 2490
Fragments arranged by size:
2490 792 778 546 190
BsmI GAATG_Cn'
Cuts at: 921 921
Size: 4796
Bsp1286I G_dGCh'C
Cuts at: 1264 1810 2421 2514 2588 2778 2840 3570 1264
Size: 546 611 93 74 190 62 730 2490
Fragments arranged by size:
2490 730 611 546 190 93 74 62
BspEI T'CCGG_A
Cuts at: 4016 4016
Size: 4796
BspHI T'CATG_A
Cuts at: 3972 3972
Size: 4796
BspMI ACCTGCnnnn'nnnn_
Cuts at: 926 1083 1846 2060 2362 2743 926
Size: 157 763 214 302 381 2979
Fragments arranged by size:
2979 763 381 302 214 157
BsrBI CCG'CTC
Cuts at: 50 3088 3142 4626 4791 50
Size: 3038 54 1484 165 55
Fragments arranged by size:
3038 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 350 614 674 1010 1696 2708 350
Size: 264 60 336 686 1012 2438
Fragments arranged by size:
2438 1012 686 336 264 60
BsrFI r'CCGG_y
Cuts at: 2794 2975 3184 2794
Size: 181 209 4406
Fragments arranged by size:
4406 209 181
BsrGI T'GTAC_A
Cuts at: 1108 1108
Size: 4796
BssSI C'ACGA_G
Cuts at: 542 3067 3425 542
Size: 2525 358 1913
Fragments arranged by size:
2525 1913 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2173 2173
Size: 4796
BstBI TT'CG_AA
Cuts at: 3157 3157
Size: 4796
BstXI CCAn_nnnn'nTGG
Cuts at: 114 447 114
Size: 333 4463
BstYI r'GATC_y
Cuts at: 1 269 363 425 1241 1821 2288 2311 2646
Size: 268 94 62 816 580 467 23 335
Cuts at: 2646 2892 3904 3990 4002 4348 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 816 580 467 449 346 335 268
246 94 86 62 23 12
Bsu36I CC'TnA_GG
Cuts at: 281 281
Size: 4796
BtgI C'CryG_G
Cuts at: 107 1776 107
Size: 1669 3127
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2746 2927 3090 2746
Size: 181 163 4452
Fragments arranged by size:
4452 181 163
BtsI GCAGTG_nn'
Cuts at: 407 1241 2039 407
Size: 834 798 3164
Fragments arranged by size:
3164 834 798
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1362 1397 1362
Size: 35 4761
DraI TTT'AAA
Cuts at: 1795 1795
Size: 4796
DrdI GACnn_nn'nnGTC
Cuts at: 2502 3360 2502
Size: 858 3938
EaeI y'GGCC_r
Cuts at: 437 1287 1498 2381 2555 2946 2973 4318 437
Size: 850 211 883 174 391 27 1345 915
Fragments arranged by size:
1345 915 883 850 391 211 174 27
EagI C'GGCC_G
Cuts at: 2381 2381
Size: 4796
EarI CTCTTCn'nnn_
Cuts at: 703 2819 3029 703
Size: 2116 210 2470
Fragments arranged by size:
2470 2116 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1551 3004 3314 3460 4606 1551
Size: 1453 310 146 1146 1741
Fragments arranged by size:
1741 1453 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1262 1808 1262
Size: 546 4250
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 173 416 445 530 1085 2621 3053 3800 4068
Size: 243 29 85 555 1536 432 747 268
Cuts at: 4068 173
Size: 901
Fragments arranged by size:
1536 901 747 555 432 268 243 85
29
EcoO109I rG'GnC_Cy
Cuts at: 118 537 1608 1935 118
Size: 419 1071 327 2979
Fragments arranged by size:
2979 1071 419 327
EcoRI G'AATT_C
Cuts at: 644 1800 644
Size: 1156 3640
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2815 2815
Size: 4796
FspI TGC'GCA
Cuts at: 2577 2577
Size: 4796
HaeII r_GCGC'y
Cuts at: 2230 2478 3500 4479 2230
Size: 248 1022 979 2547
Fragments arranged by size:
2547 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 376 408 881 913 2638 2668 2670 2700 376
Size: 32 473 32 1725 30 2 30 2472
Fragments arranged by size:
2472 1725 473 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1835 1835
Size: 4796
KasI G'GCGC_C
Cuts at: 2226 2474 2226
Size: 248 4548
KpnI G_GTAC'C
Cuts at: 1816 1816
Size: 4796
MluI A'CGCG_T
Cuts at: 4229 4229
Size: 4796
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3467 3651 3467
Size: 184 4612
MscI TGG'CCA
Cuts at: 439 1289 2557 439
Size: 850 1268 2678
Fragments arranged by size:
2678 1268 850
MslI CAynn'nnrTG
Cuts at: 445 757 1579 2785 2912 445
Size: 312 822 1206 127 2329
Fragments arranged by size:
2329 1206 822 312 127
MspA1I CmG'CkG
Cuts at: 465 1907 2221 2581 3594 3839 465
Size: 1442 314 360 1013 245 1422
Fragments arranged by size:
1442 1422 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2977 2977
Size: 4796
NarI GG'CG_CC
Cuts at: 2227 2475 2227
Size: 248 4548
NcoI C'CATG_G
Cuts at: 107 107
Size: 4796
NgoMIV G'CCGG_C
Cuts at: 2975 2975
Size: 4796
NspI r_CATG'y
Cuts at: 594 828 915 1621 1713 1849 2349 3256 594
Size: 234 87 706 92 136 500 907 2134
Fragments arranged by size:
2134 907 706 500 234 136 92 87
PasI CC'CwG_GG
Cuts at: 226 226
Size: 4796
PciI A'CATG_T
Cuts at: 911 3252 911
Size: 2341 2455
PfoI T'CCnGG_A
Cuts at: 366 1604 1718 366
Size: 1238 114 3444
Fragments arranged by size:
3444 1238 114
PmeI GTTT'AAAC
Cuts at: 1795 1795
Size: 4796
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3935 3967 3935
Size: 32 4764
PpuMI rG'GwC_Cy
Cuts at: 118 537 1608 118
Size: 419 1071 3306
Fragments arranged by size:
3306 1071 419
PstI C_TGCA'G
Cuts at: 1254 1843 2528 1254
Size: 589 685 3522
Fragments arranged by size:
3522 685 589
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4796
PvuII CAG'CTG
Cuts at: 2221 2581 2221
Size: 360 4436
RsrII CG'GwC_CG
Cuts at: 2991 2991
Size: 4796
SacI G_AGCT'C
Cuts at: 1264 1810 1264
Size: 546 4250
SalI G'TCGA_C
Cuts at: 1833 1833
Size: 4796
SapI GCTCTTCn'nnn_
Cuts at: 2819 3029 2819
Size: 210 4586
SbfI CC_TGCA'GG
Cuts at: 1843 1843
Size: 4796
ScaI AGT'ACT
Cuts at: 244 292 991 4313 244
Size: 48 699 3322 727
Fragments arranged by size:
3322 727 699 48
SexAI A'CCwGG_T
Cuts at: 333 333
Size: 4796
SfcI C'TryA_G
Cuts at: 33 198 635 1250 1441 1839 2524 3517 3708
Size: 165 437 615 191 398 685 993 191
Cuts at: 3708 33
Size: 1121
Fragments arranged by size:
1121 993 685 615 437 398 191 191
165
SfoI GGC'GCC
Cuts at: 2228 2476 2228
Size: 248 4548
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4796
SmaI CCC'GGG
Cuts at: 1818 1818
Size: 4796
SmlI C'TyrA_G
Cuts at: 817 1951 2866 3358 3620 817
Size: 1134 915 492 262 1993
Fragments arranged by size:
1993 1134 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4796
SphI G_CATG'C
Cuts at: 1849 2349 1849
Size: 500 4296
SspI AAT'ATT
Cuts at: 375 375
Size: 4796
StuI AGG'CCT
Cuts at: 1754 1754
Size: 4796
StyI C'CwwG_G
Cuts at: 107 533 1611 1930 2284 107
Size: 426 1078 319 354 2619
Fragments arranged by size:
2619 1078 426 354 319
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2379 2512 2971 3180 2379
Size: 133 459 209 3995
Fragments arranged by size:
3995 459 209 133
TatI w'GTAC_w
Cuts at: 242 290 989 1108 1583 4311 242
Size: 48 699 119 475 2728 727
Fragments arranged by size:
2728 727 699 475 119 48
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 533 565 4313 4345 533
Size: 32 3748 32 984
Fragments arranged by size:
3748 984 32 32
Tth111I GACn'n_nGTC
Cuts at: 2593 2593
Size: 4796
XbaI T'CTAG_A
Cuts at: 940 1827 940
Size: 887 3909
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1092 2291 1092
Size: 1199 3597
XmaI C'CCGG_G
Cuts at: 1816 1816
Size: 4796
XmnI GAAnn'nnTTC
Cuts at: 1281 1281
Size: 4796
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI Bsu36I
BtgI BtgZI BtsI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PfoI PmeI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI ApaI AscI
BbvCI BfrBI BglI BmgBI BmtI Bpu10I BsaXI BsiWI
BsmBI BssHII BstEII BstZ17I ClaI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PflMI PmlI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SfiI SgrAI SnaBI SrfI
SwaI XhoI ZraI