(Circular) (Six-base) MAPSORT of: pF1KB3655.seq Check: 8292 from: 1 to: 4796 pF1KB3655 4796 bp With 182 enzymes: SgfI * April 24, 2009 13:02 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 1083 1083 Size: 4796 AccI GT'mk_AC Cuts at: 1834 1834 Size: 4796 Acc65I G'GTAC_C Cuts at: 1812 1812 Size: 4796 AclI AA'CG_TT Cuts at: 4636 4636 Size: 4796 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 530 1085 2621 3053 3800 4068 530 Size: 555 1536 432 747 268 1258 Fragments arranged by size: 1536 1258 747 555 432 268 AflIII A'CryG_T Cuts at: 911 2148 3252 4229 911 Size: 1237 1104 977 1478 Fragments arranged by size: 1478 1237 1104 977 AgeI A'CCGG_T Cuts at: 3184 3184 Size: 4796 AleI CACnn'nnGTG Cuts at: 445 445 Size: 4796 AlwNI CAG_nnn'CTG Cuts at: 406 2101 3668 406 Size: 1695 1567 1534 Fragments arranged by size: 1695 1567 1534 ApaLI G'TGCA_C Cuts at: 3566 3566 Size: 4796 ApoI r'AATT_y Cuts at: 644 1800 4149 4405 644 Size: 1156 2349 256 1035 Fragments arranged by size: 2349 1156 1035 256 AseI AT'TA_AT Cuts at: 20 20 Size: 4796 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4796 AvaI C'yCGr_G Cuts at: 1337 1816 1337 Size: 479 4317 AvrII C'CTAG_G Cuts at: 1611 1611 Size: 4796 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 674 707 674 Size: 33 4763 BamHI G'GATC_C Cuts at: 363 425 1241 1821 363 Size: 62 816 580 3338 Fragments arranged by size: 3338 816 580 62 BanI G'GyrC_C Cuts at: 1812 2226 2474 2509 1812 Size: 414 248 35 4099 Fragments arranged by size: 4099 414 248 35 BanII G_rGCy'C Cuts at: 1264 1810 2840 1264 Size: 546 1030 3220 Fragments arranged by size: 3220 1030 546 BbeI G_GCGC'C Cuts at: 2230 2478 2230 Size: 248 4548 BbsI GAAGACnn'nnnn_ Cuts at: 1383 1383 Size: 4796 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 955 989 955 Size: 34 4762 BciVI GTATCCnnnnn_n' Cuts at: 539 2694 3455 4796 539 Size: 2155 761 1341 539 Fragments arranged by size: 2155 1341 761 539 BclI T'GATC_A Cuts at: 2316 2316 Size: 4796 BglII A'GATC_T Cuts at: 269 2311 4348 269 Size: 2042 2037 717 Fragments arranged by size: 2042 2037 717 BlpI GC'TnA_GC Cuts at: 609 1908 609 Size: 1299 3497 Bme1580I G_kGCm'C Cuts at: 2421 2514 3570 2421 Size: 93 1056 3647 Fragments arranged by size: 3647 1056 93 BmrI ACTGGGnnnn_n' Cuts at: 490 503 1714 2008 2089 2424 4568 490 Size: 13 1211 294 81 335 2144 718 Fragments arranged by size: 2144 1211 718 335 294 81 13 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 288 309 3163 288 Size: 21 2854 1921 Fragments arranged by size: 2854 1921 21 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 173 416 445 173 Size: 243 29 4524 Fragments arranged by size: 4524 243 29 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 802 1972 2851 3343 3641 802 Size: 1170 879 492 298 1957 Fragments arranged by size: 1957 1170 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 4500 4500 Size: 4796 BsaAI yAC'GTr Cuts at: 2149 2149 Size: 4796 BsaBI GATnn'nnATC Cuts at: 6 2310 4347 6 Size: 2304 2037 455 Fragments arranged by size: 2304 2037 455 BsaHI Gr'CG_yC Cuts at: 2227 2475 3177 4673 2227 Size: 248 702 1496 2350 Fragments arranged by size: 2350 1496 702 248 BsaWI w'CCGG_w Cuts at: 328 953 1985 2506 3184 3458 3605 4016 328 Size: 625 1032 521 678 274 147 411 1108 Fragments arranged by size: 1108 1032 678 625 521 411 274 147 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 253 298 301 253 Size: 45 3 4748 Fragments arranged by size: 4748 45 3 BseYI C'CCAG_C Cuts at: 114 799 1194 2221 2979 3556 4052 114 Size: 685 395 1027 758 577 496 858 Fragments arranged by size: 1027 858 758 685 577 496 395 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1202 1202 Size: 4796 BsiEI CG_ry'CG Cuts at: 104 2338 2384 3592 104 Size: 2234 46 1208 1308 Fragments arranged by size: 2234 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 1264 1810 2588 2778 3570 1264 Size: 546 778 190 792 2490 Fragments arranged by size: 2490 792 778 546 190 BsmI GAATG_Cn' Cuts at: 921 921 Size: 4796 Bsp1286I G_dGCh'C Cuts at: 1264 1810 2421 2514 2588 2778 2840 3570 1264 Size: 546 611 93 74 190 62 730 2490 Fragments arranged by size: 2490 730 611 546 190 93 74 62 BspEI T'CCGG_A Cuts at: 4016 4016 Size: 4796 BspHI T'CATG_A Cuts at: 3972 3972 Size: 4796 BspMI ACCTGCnnnn'nnnn_ Cuts at: 926 1083 1846 2060 2362 2743 926 Size: 157 763 214 302 381 2979 Fragments arranged by size: 2979 763 381 302 214 157 BsrBI CCG'CTC Cuts at: 50 3088 3142 4626 4791 50 Size: 3038 54 1484 165 55 Fragments arranged by size: 3038 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 350 614 674 1010 1696 2708 350 Size: 264 60 336 686 1012 2438 Fragments arranged by size: 2438 1012 686 336 264 60 BsrFI r'CCGG_y Cuts at: 2794 2975 3184 2794 Size: 181 209 4406 Fragments arranged by size: 4406 209 181 BsrGI T'GTAC_A Cuts at: 1108 1108 Size: 4796 BssSI C'ACGA_G Cuts at: 542 3067 3425 542 Size: 2525 358 1913 Fragments arranged by size: 2525 1913 358 BstAPI GCAn_nnn'nTGC Cuts at: 2173 2173 Size: 4796 BstBI TT'CG_AA Cuts at: 3157 3157 Size: 4796 BstXI CCAn_nnnn'nTGG Cuts at: 114 447 114 Size: 333 4463 BstYI r'GATC_y Cuts at: 1 269 363 425 1241 1821 2288 2311 2646 Size: 268 94 62 816 580 467 23 335 Cuts at: 2646 2892 3904 3990 4002 4348 1 Size: 246 1012 86 12 346 449 Fragments arranged by size: 1012 816 580 467 449 346 335 268 246 94 86 62 23 12 Bsu36I CC'TnA_GG Cuts at: 281 281 Size: 4796 BtgI C'CryG_G Cuts at: 107 1776 107 Size: 1669 3127 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 2746 2927 3090 2746 Size: 181 163 4452 Fragments arranged by size: 4452 181 163 BtsI GCAGTG_nn' Cuts at: 407 1241 2039 407 Size: 834 798 3164 Fragments arranged by size: 3164 834 798 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 1362 1397 1362 Size: 35 4761 DraI TTT'AAA Cuts at: 1795 1795 Size: 4796 DrdI GACnn_nn'nnGTC Cuts at: 2502 3360 2502 Size: 858 3938 EaeI y'GGCC_r Cuts at: 437 1287 1498 2381 2555 2946 2973 4318 437 Size: 850 211 883 174 391 27 1345 915 Fragments arranged by size: 1345 915 883 850 391 211 174 27 EagI C'GGCC_G Cuts at: 2381 2381 Size: 4796 EarI CTCTTCn'nnn_ Cuts at: 703 2819 3029 703 Size: 2116 210 2470 Fragments arranged by size: 2470 2116 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 1551 3004 3314 3460 4606 1551 Size: 1453 310 146 1146 1741 Fragments arranged by size: 1741 1453 1146 310 146 EcoICRI GAG'CTC Cuts at: 1262 1808 1262 Size: 546 4250 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 173 416 445 530 1085 2621 3053 3800 4068 Size: 243 29 85 555 1536 432 747 268 Cuts at: 4068 173 Size: 901 Fragments arranged by size: 1536 901 747 555 432 268 243 85 29 EcoO109I rG'GnC_Cy Cuts at: 118 537 1608 1935 118 Size: 419 1071 327 2979 Fragments arranged by size: 2979 1071 419 327 EcoRI G'AATT_C Cuts at: 644 1800 644 Size: 1156 3640 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2815 2815 Size: 4796 FspI TGC'GCA Cuts at: 2577 2577 Size: 4796 HaeII r_GCGC'y Cuts at: 2230 2478 3500 4479 2230 Size: 248 1022 979 2547 Fragments arranged by size: 2547 1022 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 376 408 881 913 2638 2668 2670 2700 376 Size: 32 473 32 1725 30 2 30 2472 Fragments arranged by size: 2472 1725 473 32 32 30 30 2 HincII GTy'rAC Cuts at: 1835 1835 Size: 4796 KasI G'GCGC_C Cuts at: 2226 2474 2226 Size: 248 4548 KpnI G_GTAC'C Cuts at: 1816 1816 Size: 4796 MluI A'CGCG_T Cuts at: 4229 4229 Size: 4796 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 3467 3651 3467 Size: 184 4612 MscI TGG'CCA Cuts at: 439 1289 2557 439 Size: 850 1268 2678 Fragments arranged by size: 2678 1268 850 MslI CAynn'nnrTG Cuts at: 445 757 1579 2785 2912 445 Size: 312 822 1206 127 2329 Fragments arranged by size: 2329 1206 822 312 127 MspA1I CmG'CkG Cuts at: 465 1907 2221 2581 3594 3839 465 Size: 1442 314 360 1013 245 1422 Fragments arranged by size: 1442 1422 1013 360 314 245 NaeI GCC'GGC Cuts at: 2977 2977 Size: 4796 NarI GG'CG_CC Cuts at: 2227 2475 2227 Size: 248 4548 NcoI C'CATG_G Cuts at: 107 107 Size: 4796 NgoMIV G'CCGG_C Cuts at: 2975 2975 Size: 4796 NspI r_CATG'y Cuts at: 594 828 915 1621 1713 1849 2349 3256 594 Size: 234 87 706 92 136 500 907 2134 Fragments arranged by size: 2134 907 706 500 234 136 92 87 PasI CC'CwG_GG Cuts at: 226 226 Size: 4796 PciI A'CATG_T Cuts at: 911 3252 911 Size: 2341 2455 PfoI T'CCnGG_A Cuts at: 366 1604 1718 366 Size: 1238 114 3444 Fragments arranged by size: 3444 1238 114 PmeI GTTT'AAAC Cuts at: 1795 1795 Size: 4796 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3935 3967 3935 Size: 32 4764 PpuMI rG'GwC_Cy Cuts at: 118 537 1608 118 Size: 419 1071 3306 Fragments arranged by size: 3306 1071 419 PstI C_TGCA'G Cuts at: 1254 1843 2528 1254 Size: 589 685 3522 Fragments arranged by size: 3522 685 589 PvuI CG_AT'CG Cuts at: 104 104 Size: 4796 PvuII CAG'CTG Cuts at: 2221 2581 2221 Size: 360 4436 RsrII CG'GwC_CG Cuts at: 2991 2991 Size: 4796 SacI G_AGCT'C Cuts at: 1264 1810 1264 Size: 546 4250 SalI G'TCGA_C Cuts at: 1833 1833 Size: 4796 SapI GCTCTTCn'nnn_ Cuts at: 2819 3029 2819 Size: 210 4586 SbfI CC_TGCA'GG Cuts at: 1843 1843 Size: 4796 ScaI AGT'ACT Cuts at: 244 292 991 4313 244 Size: 48 699 3322 727 Fragments arranged by size: 3322 727 699 48 SexAI A'CCwGG_T Cuts at: 333 333 Size: 4796 SfcI C'TryA_G Cuts at: 33 198 635 1250 1441 1839 2524 3517 3708 Size: 165 437 615 191 398 685 993 191 Cuts at: 3708 33 Size: 1121 Fragments arranged by size: 1121 993 685 615 437 398 191 191 165 SfoI GGC'GCC Cuts at: 2228 2476 2228 Size: 248 4548 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4796 SmaI CCC'GGG Cuts at: 1818 1818 Size: 4796 SmlI C'TyrA_G Cuts at: 817 1951 2866 3358 3620 817 Size: 1134 915 492 262 1993 Fragments arranged by size: 1993 1134 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 4796 SphI G_CATG'C Cuts at: 1849 2349 1849 Size: 500 4296 SspI AAT'ATT Cuts at: 375 375 Size: 4796 StuI AGG'CCT Cuts at: 1754 1754 Size: 4796 StyI C'CwwG_G Cuts at: 107 533 1611 1930 2284 107 Size: 426 1078 319 354 2619 Fragments arranged by size: 2619 1078 426 354 319 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 2379 2512 2971 3180 2379 Size: 133 459 209 3995 Fragments arranged by size: 3995 459 209 133 TatI w'GTAC_w Cuts at: 242 290 989 1108 1583 4311 242 Size: 48 699 119 475 2728 727 Fragments arranged by size: 2728 727 699 475 119 48 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 533 565 4313 4345 533 Size: 32 3748 32 984 Fragments arranged by size: 3748 984 32 32 Tth111I GACn'n_nGTC Cuts at: 2593 2593 Size: 4796 XbaI T'CTAG_A Cuts at: 940 1827 940 Size: 887 3909 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1092 2291 1092 Size: 1199 3597 XmaI C'CCGG_G Cuts at: 1816 1816 Size: 4796 XmnI GAAnn'nnTTC Cuts at: 1281 1281 Size: 4796 Enzymes that do cut: AarI AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI PfoI PmeI PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI Enzymes that do not cut: AatII AfeI AflII AhdI AlfI AloI ApaI AscI BbvCI BfrBI BglI BmgBI BmtI Bpu10I BsaXI BsiWI BsmBI BssHII BstEII BstZ17I ClaI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI NotI NruI NsiI PacI PflMI PmlI PshAI PsiI PspOMI PspXI PsrI SacII SanDI SfiI SgrAI SnaBI SrfI SwaI XhoI ZraI