Restriction map for pF1KB3655
 (Circular) (Six-base) MAPSORT of: pF1KB3655.seq  Check: 8292  from: 1  to: 4796



pF1KB3655  4796 bp

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:02  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   1083    1083 
   Size:       4796
 
AccI GT'mk_AC

Cuts at:   1834    1834 
   Size:       4796
 
Acc65I G'GTAC_C

Cuts at:   1812    1812 
   Size:       4796
 
AclI AA'CG_TT

Cuts at:   4636    4636 
   Size:       4796
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    530    1085    2621    3053    3800    4068     530 
   Size:        555    1536     432     747     268    1258

  Fragments arranged by size: 

                1536    1258     747     555     432     268
 
AflIII A'CryG_T

Cuts at:    911    2148    3252    4229     911 
   Size:       1237    1104     977    1478

  Fragments arranged by size: 

                1478    1237    1104     977
 
AgeI A'CCGG_T

Cuts at:   3184    3184 
   Size:       4796
 
AleI CACnn'nnGTG

Cuts at:    445     445 
   Size:       4796
 
AlwNI CAG_nnn'CTG

Cuts at:    406    2101    3668     406 
   Size:       1695    1567    1534

  Fragments arranged by size: 

                1695    1567    1534
 
ApaLI G'TGCA_C

Cuts at:   3566    3566 
   Size:       4796
 
ApoI r'AATT_y

Cuts at:    644    1800    4149    4405     644 
   Size:       1156    2349     256    1035

  Fragments arranged by size: 

                2349    1156    1035     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4796
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4796
 
AvaI C'yCGr_G

Cuts at:   1337    1816    1337 
   Size:        479    4317
 
AvrII C'CTAG_G

Cuts at:   1611    1611 
   Size:       4796
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:    674     707     674 
   Size:         33    4763
 
BamHI G'GATC_C

Cuts at:    363     425    1241    1821     363 
   Size:         62     816     580    3338

  Fragments arranged by size: 

                3338     816     580      62
 
BanI G'GyrC_C

Cuts at:   1812    2226    2474    2509    1812 
   Size:        414     248      35    4099

  Fragments arranged by size: 

                4099     414     248      35
 
BanII G_rGCy'C

Cuts at:   1264    1810    2840    1264 
   Size:        546    1030    3220

  Fragments arranged by size: 

                3220    1030     546
 
BbeI G_GCGC'C

Cuts at:   2230    2478    2230 
   Size:        248    4548
 
BbsI GAAGACnn'nnnn_

Cuts at:   1383    1383 
   Size:       4796
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    955     989     955 
   Size:         34    4762
 
BciVI GTATCCnnnnn_n'

Cuts at:    539    2694    3455    4796     539 
   Size:       2155     761    1341     539

  Fragments arranged by size: 

                2155    1341     761     539
 
BclI T'GATC_A

Cuts at:   2316    2316 
   Size:       4796
 
BglII A'GATC_T

Cuts at:    269    2311    4348     269 
   Size:       2042    2037     717

  Fragments arranged by size: 

                2042    2037     717
 
BlpI GC'TnA_GC

Cuts at:    609    1908     609 
   Size:       1299    3497
 
Bme1580I G_kGCm'C

Cuts at:   2421    2514    3570    2421 
   Size:         93    1056    3647

  Fragments arranged by size: 

                3647    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    490     503    1714    2008    2089    2424    4568     490 
   Size:         13    1211     294      81     335    2144     718

  Fragments arranged by size: 

                2144    1211     718     335     294      81      13
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    288     309    3163     288 
   Size:         21    2854    1921

  Fragments arranged by size: 

                2854    1921      21
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    173     416     445     173 
   Size:        243      29    4524

  Fragments arranged by size: 

                4524     243      29
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    802    1972    2851    3343    3641     802 
   Size:       1170     879     492     298    1957

  Fragments arranged by size: 

                1957    1170     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   4500    4500 
   Size:       4796
 
BsaAI yAC'GTr

Cuts at:   2149    2149 
   Size:       4796
 
BsaBI GATnn'nnATC

Cuts at:      6    2310    4347       6 
   Size:       2304    2037     455

  Fragments arranged by size: 

                2304    2037     455
 
BsaHI Gr'CG_yC

Cuts at:   2227    2475    3177    4673    2227 
   Size:        248     702    1496    2350

  Fragments arranged by size: 

                2350    1496     702     248
 
BsaWI w'CCGG_w

Cuts at:    328     953    1985    2506    3184    3458    3605    4016     328 
   Size:        625    1032     521     678     274     147     411    1108

  Fragments arranged by size: 

                1108    1032     678     625     521     411     274     147
            
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    253     298     301     253 
   Size:         45       3    4748

  Fragments arranged by size: 

                4748      45       3
 
BseYI C'CCAG_C

Cuts at:    114     799    1194    2221    2979    3556    4052     114 
   Size:        685     395    1027     758     577     496     858

  Fragments arranged by size: 

                1027     858     758     685     577     496     395
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1202    1202 
   Size:       4796
 
BsiEI CG_ry'CG

Cuts at:    104    2338    2384    3592     104 
   Size:       2234      46    1208    1308

  Fragments arranged by size: 

                2234    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:   1264    1810    2588    2778    3570    1264 
   Size:        546     778     190     792    2490

  Fragments arranged by size: 

                2490     792     778     546     190
 
BsmI GAATG_Cn'

Cuts at:    921     921 
   Size:       4796
 
Bsp1286I G_dGCh'C

Cuts at:   1264    1810    2421    2514    2588    2778    2840    3570    1264 
   Size:        546     611      93      74     190      62     730    2490

  Fragments arranged by size: 

                2490     730     611     546     190      93      74      62
            
 
BspEI T'CCGG_A

Cuts at:   4016    4016 
   Size:       4796
 
BspHI T'CATG_A

Cuts at:   3972    3972 
   Size:       4796
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    926    1083    1846    2060    2362    2743     926 
   Size:        157     763     214     302     381    2979

  Fragments arranged by size: 

                2979     763     381     302     214     157
 
BsrBI CCG'CTC

Cuts at:     50    3088    3142    4626    4791      50 
   Size:       3038      54    1484     165      55

  Fragments arranged by size: 

                3038    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:    350     614     674    1010    1696    2708     350 
   Size:        264      60     336     686    1012    2438

  Fragments arranged by size: 

                2438    1012     686     336     264      60
 
BsrFI r'CCGG_y

Cuts at:   2794    2975    3184    2794 
   Size:        181     209    4406

  Fragments arranged by size: 

                4406     209     181
 
BsrGI T'GTAC_A

Cuts at:   1108    1108 
   Size:       4796
 
BssSI C'ACGA_G

Cuts at:    542    3067    3425     542 
   Size:       2525     358    1913

  Fragments arranged by size: 

                2525    1913     358
 
BstAPI GCAn_nnn'nTGC

Cuts at:   2173    2173 
   Size:       4796
 
BstBI TT'CG_AA

Cuts at:   3157    3157 
   Size:       4796
 
BstXI CCAn_nnnn'nTGG

Cuts at:    114     447     114 
   Size:        333    4463
 
BstYI r'GATC_y

Cuts at:      1     269     363     425    1241    1821    2288    2311    2646 
   Size:        268      94      62     816     580     467      23     335

Cuts at:   2646    2892    3904    3990    4002    4348       1 
   Size:        246    1012      86      12     346     449

  Fragments arranged by size: 

                1012     816     580     467     449     346     335     268
                 246      94      86      62      23      12
 
Bsu36I CC'TnA_GG

Cuts at:    281     281 
   Size:       4796
 
BtgI C'CryG_G

Cuts at:    107    1776     107 
   Size:       1669    3127
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   2746    2927    3090    2746 
   Size:        181     163    4452

  Fragments arranged by size: 

                4452     181     163
 
BtsI GCAGTG_nn'

Cuts at:    407    1241    2039     407 
   Size:        834     798    3164

  Fragments arranged by size: 

                3164     834     798
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:   1362    1397    1362 
   Size:         35    4761
 
DraI TTT'AAA

Cuts at:   1795    1795 
   Size:       4796
 
DrdI GACnn_nn'nnGTC

Cuts at:   2502    3360    2502 
   Size:        858    3938
 
EaeI y'GGCC_r

Cuts at:    437    1287    1498    2381    2555    2946    2973    4318     437 
   Size:        850     211     883     174     391      27    1345     915

  Fragments arranged by size: 

                1345     915     883     850     391     211     174      27
            
 
EagI C'GGCC_G

Cuts at:   2381    2381 
   Size:       4796
 
EarI CTCTTCn'nnn_

Cuts at:    703    2819    3029     703 
   Size:       2116     210    2470

  Fragments arranged by size: 

                2470    2116     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   1551    3004    3314    3460    4606    1551 
   Size:       1453     310     146    1146    1741

  Fragments arranged by size: 

                1741    1453    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:   1262    1808    1262 
   Size:        546    4250
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    173     416     445     530    1085    2621    3053    3800    4068 
   Size:        243      29      85     555    1536     432     747     268

Cuts at:   4068     173 
   Size:        901

  Fragments arranged by size: 

                1536     901     747     555     432     268     243      85
                  29
 
EcoO109I rG'GnC_Cy

Cuts at:    118     537    1608    1935     118 
   Size:        419    1071     327    2979

  Fragments arranged by size: 

                2979    1071     419     327
 
EcoRI G'AATT_C

Cuts at:    644    1800     644 
   Size:       1156    3640
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2815    2815 
   Size:       4796
 
FspI TGC'GCA

Cuts at:   2577    2577 
   Size:       4796
 
HaeII r_GCGC'y

Cuts at:   2230    2478    3500    4479    2230 
   Size:        248    1022     979    2547

  Fragments arranged by size: 

                2547    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    376     408     881     913    2638    2668    2670    2700     376 
   Size:         32     473      32    1725      30       2      30    2472

  Fragments arranged by size: 

                2472    1725     473      32      32      30      30       2
            
 
HincII GTy'rAC

Cuts at:   1835    1835 
   Size:       4796
 
KasI G'GCGC_C

Cuts at:   2226    2474    2226 
   Size:        248    4548
 
KpnI G_GTAC'C

Cuts at:   1816    1816 
   Size:       4796
 
MluI A'CGCG_T

Cuts at:   4229    4229 
   Size:       4796
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   3467    3651    3467 
   Size:        184    4612
 
MscI TGG'CCA

Cuts at:    439    1289    2557     439 
   Size:        850    1268    2678

  Fragments arranged by size: 

                2678    1268     850
 
MslI CAynn'nnrTG

Cuts at:    445     757    1579    2785    2912     445 
   Size:        312     822    1206     127    2329

  Fragments arranged by size: 

                2329    1206     822     312     127
 
MspA1I CmG'CkG

Cuts at:    465    1907    2221    2581    3594    3839     465 
   Size:       1442     314     360    1013     245    1422

  Fragments arranged by size: 

                1442    1422    1013     360     314     245
 
NaeI GCC'GGC

Cuts at:   2977    2977 
   Size:       4796
 
NarI GG'CG_CC

Cuts at:   2227    2475    2227 
   Size:        248    4548
 
NcoI C'CATG_G

Cuts at:    107     107 
   Size:       4796
 
NgoMIV G'CCGG_C

Cuts at:   2975    2975 
   Size:       4796
 
NspI r_CATG'y

Cuts at:    594     828     915    1621    1713    1849    2349    3256     594 
   Size:        234      87     706      92     136     500     907    2134

  Fragments arranged by size: 

                2134     907     706     500     234     136      92      87
            
 
PasI CC'CwG_GG

Cuts at:    226     226 
   Size:       4796
 
PciI A'CATG_T

Cuts at:    911    3252     911 
   Size:       2341    2455
 
PfoI T'CCnGG_A

Cuts at:    366    1604    1718     366 
   Size:       1238     114    3444

  Fragments arranged by size: 

                3444    1238     114
 
PmeI GTTT'AAAC

Cuts at:   1795    1795 
   Size:       4796
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3935    3967    3935 
   Size:         32    4764
 
PpuMI rG'GwC_Cy

Cuts at:    118     537    1608     118 
   Size:        419    1071    3306

  Fragments arranged by size: 

                3306    1071     419
 
PstI C_TGCA'G

Cuts at:   1254    1843    2528    1254 
   Size:        589     685    3522

  Fragments arranged by size: 

                3522     685     589
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4796
 
PvuII CAG'CTG

Cuts at:   2221    2581    2221 
   Size:        360    4436
 
RsrII CG'GwC_CG

Cuts at:   2991    2991 
   Size:       4796
 
SacI G_AGCT'C

Cuts at:   1264    1810    1264 
   Size:        546    4250
 
SalI G'TCGA_C

Cuts at:   1833    1833 
   Size:       4796
 
SapI GCTCTTCn'nnn_

Cuts at:   2819    3029    2819 
   Size:        210    4586
 
SbfI CC_TGCA'GG

Cuts at:   1843    1843 
   Size:       4796
 
ScaI AGT'ACT

Cuts at:    244     292     991    4313     244 
   Size:         48     699    3322     727

  Fragments arranged by size: 

                3322     727     699      48
 
SexAI A'CCwGG_T

Cuts at:    333     333 
   Size:       4796
 
SfcI C'TryA_G

Cuts at:     33     198     635    1250    1441    1839    2524    3517    3708 
   Size:        165     437     615     191     398     685     993     191

Cuts at:   3708      33 
   Size:       1121

  Fragments arranged by size: 

                1121     993     685     615     437     398     191     191
                 165
 
SfoI GGC'GCC

Cuts at:   2228    2476    2228 
   Size:        248    4548
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4796
 
SmaI CCC'GGG

Cuts at:   1818    1818 
   Size:       4796
 
SmlI C'TyrA_G

Cuts at:    817    1951    2866    3358    3620     817 
   Size:       1134     915     492     262    1993

  Fragments arranged by size: 

                1993    1134     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4796
 
SphI G_CATG'C

Cuts at:   1849    2349    1849 
   Size:        500    4296
 
SspI AAT'ATT

Cuts at:    375     375 
   Size:       4796
 
StuI AGG'CCT

Cuts at:   1754    1754 
   Size:       4796
 
StyI C'CwwG_G

Cuts at:    107     533    1611    1930    2284     107 
   Size:        426    1078     319     354    2619

  Fragments arranged by size: 

                2619    1078     426     354     319
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   2379    2512    2971    3180    2379 
   Size:        133     459     209    3995

  Fragments arranged by size: 

                3995     459     209     133
 
TatI w'GTAC_w

Cuts at:    242     290     989    1108    1583    4311     242 
   Size:         48     699     119     475    2728     727

  Fragments arranged by size: 

                2728     727     699     475     119      48
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    533     565    4313    4345     533 
   Size:         32    3748      32     984

  Fragments arranged by size: 

                3748     984      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   2593    2593 
   Size:       4796
 
XbaI T'CTAG_A

Cuts at:    940    1827     940 
   Size:        887    3909
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1092    2291    1092 
   Size:       1199    3597
 
XmaI C'CCGG_G

Cuts at:   1816    1816 
   Size:       4796
 
XmnI GAAnn'nnTTC

Cuts at:   1281    1281 
   Size:       4796
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AleI
    AlwNI    ApaLI     ApoI     AseI    AsiSI     AvaI    AvrII     BaeI
    BamHI     BanI    BanII     BbeI     BbsI     BcgI    BciVI     BclI
    BglII     BlpI Bme1580I     BmrI     BplI     BpmI    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI    BssSI   BstAPI    BstBI    BstXI    BstYI   Bsu36I
     BtgI    BtgZI     BtsI    CspCI     DraI     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI     FspI
    HaeII    Hin4I   HincII     KasI     KpnI     MluI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI     PasI
     PciI     PfoI     PmeI     PpiI    PpuMI     PstI     PvuI    PvuII
    RsrII     SacI     SalI     SapI     SbfI     ScaI    SexAI     SfcI
     SfoI     SgfI     SmaI     SmlI     SpeI     SphI     SspI     StuI
     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XmaI
     XmnI

 Enzymes that do not cut: 

    AatII     AfeI    AflII     AhdI     AlfI     AloI     ApaI     AscI
    BbvCI    BfrBI     BglI    BmgBI     BmtI   Bpu10I    BsaXI    BsiWI
    BsmBI   BssHII   BstEII  BstZ17I     ClaI   DraIII    EcoNI    EcoRV
     FseI    FspAI  HindIII     HpaI     MfeI     NdeI     NheI     NotI
     NruI     NsiI     PacI    PflMI     PmlI    PshAI     PsiI   PspOMI
    PspXI     PsrI    SacII    SanDI     SfiI    SgrAI    SnaBI     SrfI
     SwaI     XhoI     ZraI
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