Restriction map for
pF1KB3654
(Circular) (Six-base) MAPSORT of: pF1KB3654.seq Check: 5186 from: 1 to: 6200
pF1KB3654 6200 bp
With 182 enzymes: SgfI *
March 2, 2010 14:25 ..
AccI GT'mk_AC
Cuts at: 3238 3238
Size: 6200
Acc65I G'GTAC_C
Cuts at: 3216 3216
Size: 6200
AclI AA'CG_TT
Cuts at: 6040 6040
Size: 6200
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 692 773 1220 1690 2110 2530 2571 4025 4457
Size: 81 447 470 420 420 41 1454 432
Cuts at: 4457 4810 5472 692
Size: 353 662 1420
Fragments arranged by size:
1454 1420 662 470 447 432 420 420
353 81 41
AflIII A'CryG_T
Cuts at: 3552 5352 5633 3552
Size: 1800 281 4119
Fragments arranged by size:
4119 1800 281
AleI CACnn'nnGTG
Cuts at: 1286 2462 1286
Size: 1176 5024
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 546 578 546
Size: 32 6168
AlwNI CAG_nnn'CTG
Cuts at: 351 378 3505 4943 351
Size: 27 3127 1438 1608
Fragments arranged by size:
3127 1608 1438 27
ApaI G_GGCC'C
Cuts at: 814 814
Size: 6200
ApaLI G'TGCA_C
Cuts at: 5038 5038
Size: 6200
ApoI r'AATT_y
Cuts at: 599 2537 2705 2937 2957 3088 3142 3204 5553
Size: 1938 168 232 20 131 54 62 2349
Cuts at: 5553 5809 599
Size: 256 990
Fragments arranged by size:
2349 1938 990 256 232 168 131 62
54 20
AseI AT'TA_AT
Cuts at: 20 715 20
Size: 695 5505
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6200
AvaI C'yCGr_G
Cuts at: 3220 3220
Size: 6200
BamHI G'GATC_C
Cuts at: 645 1445 2621 3225 645
Size: 800 1176 604 3620
Fragments arranged by size:
3620 1176 800 604
BanI G'GyrC_C
Cuts at: 1529 3216 3630 3878 3913 1529
Size: 1687 414 248 35 3816
Fragments arranged by size:
3816 1687 414 248 35
BanII G_rGCy'C
Cuts at: 814 3214 4244 814
Size: 2400 1030 2770
Fragments arranged by size:
2770 2400 1030
BbeI G_GCGC'C
Cuts at: 1533 3634 3882 1533
Size: 2101 248 3851
Fragments arranged by size:
3851 2101 248
BbsI GAAGACnn'nnnn_
Cuts at: 1569 2157 2577 1569
Size: 588 420 5192
Fragments arranged by size:
5192 588 420
BbvCI CC'TCA_GC
Cuts at: 650 650
Size: 6200
BciVI GTATCCnnnnn_n'
Cuts at: 4098 5154 6200 4098
Size: 1056 1046 4098
Fragments arranged by size:
4098 1056 1046
BclI T'GATC_A
Cuts at: 3720 3720
Size: 6200
BfrBI ATG'CAT
Cuts at: 1353 1353
Size: 6200
BglII A'GATC_T
Cuts at: 3715 5752 3715
Size: 2037 4163
BlpI GC'TnA_GC
Cuts at: 3312 3312
Size: 6200
Bme1580I G_kGCm'C
Cuts at: 549 814 3825 3918 5042 549
Size: 265 3011 93 1124 1707
Fragments arranged by size:
3011 1707 1124 265 93
BmrI ACTGGGnnnn_n'
Cuts at: 843 1463 2891 3412 3493 3828 5972 843
Size: 620 1428 521 81 335 2144 1071
Fragments arranged by size:
2144 1428 1071 620 521 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2152 3162 4567 2152
Size: 1010 1405 3785
Fragments arranged by size:
3785 1405 1010
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 373 388 514 625 868 2147 2819 373
Size: 15 126 111 243 1279 672 3754
Fragments arranged by size:
3754 1279 672 243 126 111 15
Bpu10I CC'TnA_GC
Cuts at: 286 295 650 286
Size: 9 355 5836
Fragments arranged by size:
5836 355 9
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3376 4255 4969 5267 3376
Size: 879 714 298 4309
Fragments arranged by size:
4309 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 217 315 877 1539 5904 217
Size: 98 562 662 4365 513
Fragments arranged by size:
4365 662 562 513 98
BsaAI yAC'GTr
Cuts at: 2675 3553 2675
Size: 878 5322
BsaBI GATnn'nnATC
Cuts at: 6 3714 5751 6
Size: 3708 2037 455
Fragments arranged by size:
3708 2037 455
BsaHI Gr'CG_yC
Cuts at: 1530 3631 3879 4581 6077 1530
Size: 2101 248 702 1496 1653
Fragments arranged by size:
2101 1653 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2377 3389 3910 4588 4999 5146 5420 2377
Size: 1012 521 678 411 147 274 3157
Fragments arranged by size:
3157 1012 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 549 579 713 743 549
Size: 30 134 30 6006
Fragments arranged by size:
6006 134 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 201 454 475 781 916 1840 201
Size: 253 21 306 135 924 4561
Fragments arranged by size:
4561 924 306 253 135 21
BseYI C'CCAG_C
Cuts at: 740 3625 4383 5048 5456 740
Size: 2885 758 665 408 1484
Fragments arranged by size:
2885 1484 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1672 1811 1672
Size: 139 6061
BsiEI CG_ry'CG
Cuts at: 104 3742 3788 5018 104
Size: 3638 46 1230 1286
Fragments arranged by size:
3638 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 3214 3992 4182 5042 3214
Size: 778 190 860 4372
Fragments arranged by size:
4372 860 778 190
BsmI GAATG_Cn'
Cuts at: 631 1593 2401 2879 631
Size: 962 808 478 3952
Fragments arranged by size:
3952 962 808 478
Bsp1286I G_dGCh'C
Cuts at: 549 814 3214 3825 3918 3992 4182 4244 5042
Size: 265 2400 611 93 74 190 62 798
Cuts at: 5042 549
Size: 1707
Fragments arranged by size:
2400 1707 798 611 265 190 93 74
62
BspHI T'CATG_A
Cuts at: 4632 4632
Size: 6200
BspMI ACCTGCnnnn'nnnn_
Cuts at: 3250 3464 3766 4147 3250
Size: 214 302 381 5303
Fragments arranged by size:
5303 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2597 4492 4546 6030 6195 50
Size: 2547 1895 54 1484 165 55
Fragments arranged by size:
2547 1895 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2574 4112 2574
Size: 1538 4662
BsrFI r'CCGG_y
Cuts at: 290 311 4198 4379 290
Size: 21 3887 181 2111
Fragments arranged by size:
3887 2111 181 21
BsrGI T'GTAC_A
Cuts at: 2358 2862 3030 2358
Size: 504 168 5528
Fragments arranged by size:
5528 504 168
BssSI C'ACGA_G
Cuts at: 2544 4471 5179 2544
Size: 1927 708 3565
Fragments arranged by size:
3565 1927 708
BstAPI GCAn_nnn'nTGC
Cuts at: 3577 3577
Size: 6200
BstBI TT'CG_AA
Cuts at: 1836 4561 1836
Size: 2725 3475
BstXI CCAn_nnnn'nTGG
Cuts at: 427 427
Size: 6200
BstYI r'GATC_y
Cuts at: 1 347 645 1445 2621 3225 3692 3715 4050
Size: 346 298 800 1176 604 467 23 335
Cuts at: 4050 4296 4602 4614 4700 5752 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
1176 1052 800 604 467 449 346 335
306 298 246 86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4150 4331 4494 4150
Size: 181 163 5856
Fragments arranged by size:
5856 181 163
BtsI GCAGTG_nn'
Cuts at: 2406 3443 2406
Size: 1037 5163
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2556 2591 2556
Size: 35 6165
DraI TTT'AAA
Cuts at: 3199 3199
Size: 6200
DrdI GACnn_nn'nnGTC
Cuts at: 3906 5250 3906
Size: 1344 4856
EaeI y'GGCC_r
Cuts at: 2946 3785 3959 4350 4377 5722 2946
Size: 839 174 391 27 1345 3424
Fragments arranged by size:
3424 1345 839 391 174 27
EagI C'GGCC_G
Cuts at: 3785 3785
Size: 6200
EarI CTCTTCn'nnn_
Cuts at: 689 998 1669 2089 2406 4223 4433 689
Size: 309 671 420 317 1817 210 2456
Fragments arranged by size:
2456 1817 671 420 317 309 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4408 5150 5296 6010 4408
Size: 742 146 714 4598
Fragments arranged by size:
4598 742 714 146
EcoICRI GAG'CTC
Cuts at: 3212 3212
Size: 6200
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 373 388 514 625 692 773 868 1220 1690
Size: 15 126 111 67 81 95 352 470
Cuts at: 1690 2110 2147 2530 2571 2819 4025 4457 4810
Size: 420 37 383 41 248 1206 432 353
Cuts at: 4810 5472 373
Size: 662 1101
Fragments arranged by size:
1206 1101 662 470 432 420 383 353
352 248 126 111 95 81 67 41
37 15
EcoNI CCTnn'n_nnAGG
Cuts at: 157 457 511 688 157
Size: 300 54 177 5669
Fragments arranged by size:
5669 300 177 54
EcoO109I rG'GnC_Cy
Cuts at: 150 824 987 3339 150
Size: 674 163 2352 3011
Fragments arranged by size:
3011 2352 674 163
EcoRI G'AATT_C
Cuts at: 2537 2705 2957 3088 3142 3204 2537
Size: 168 252 131 54 62 5533
Fragments arranged by size:
5533 252 168 131 62 54
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 169 1099 1902 1947 4219 169
Size: 930 803 45 2272 2150
Fragments arranged by size:
2272 2150 930 803 45
FspI TGC'GCA
Cuts at: 3981 3981
Size: 6200
HaeII r_GCGC'y
Cuts at: 1533 3634 3882 5112 5883 1533
Size: 2101 248 1230 771 1850
Fragments arranged by size:
2101 1850 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 757 789 895 927 1734 1746 1766 1778 2682
Size: 32 106 32 807 12 20 12 904
Cuts at: 2682 2714 4042 4072 4074 4104 757
Size: 32 1328 30 2 30 2853
Fragments arranged by size:
2853 1328 904 807 106 32 32 32
30 30 20 12 12 2
HincII GTy'rAC
Cuts at: 3239 3239
Size: 6200
HindIII A'AGCT_T
Cuts at: 674 1658 674
Size: 984 5216
KasI G'GCGC_C
Cuts at: 1529 3630 3878 1529
Size: 2101 248 3851
Fragments arranged by size:
3851 2101 248
KpnI G_GTAC'C
Cuts at: 3220 3220
Size: 6200
MluI A'CGCG_T
Cuts at: 5633 5633
Size: 6200
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3128 4959 5143 3128
Size: 1831 184 4185
Fragments arranged by size:
4185 1831 184
MscI TGG'CCA
Cuts at: 2948 3961 2948
Size: 1013 5187
MslI CAynn'nnrTG
Cuts at: 1286 2226 2462 2630 3050 4189 4316 1286
Size: 940 236 168 420 1139 127 3170
Fragments arranged by size:
3170 1139 940 420 236 168 127
MspA1I CmG'CkG
Cuts at: 595 3311 3625 3985 4769 5014 595
Size: 2716 314 360 784 245 1781
Fragments arranged by size:
2716 1781 784 360 314 245
NaeI GCC'GGC
Cuts at: 292 4381 292
Size: 4089 2111
NarI GG'CG_CC
Cuts at: 1530 3631 3879 1530
Size: 2101 248 3851
Fragments arranged by size:
3851 2101 248
NdeI CA'TA_TG
Cuts at: 2223 2223
Size: 6200
NgoMIV G'CCGG_C
Cuts at: 290 4379 290
Size: 4089 2111
NsiI A_TGCA'T
Cuts at: 1355 1355
Size: 6200
NspI r_CATG'y
Cuts at: 806 3253 3753 5356 806
Size: 2447 500 1603 1650
Fragments arranged by size:
2447 1650 1603 500
PciI A'CATG_T
Cuts at: 5352 5352
Size: 6200
PflMI CCAn_nnn'nTGG
Cuts at: 537 753 537
Size: 216 5984
PfoI T'CCnGG_A
Cuts at: 350 641 350
Size: 291 5909
PmeI GTTT'AAAC
Cuts at: 3199 3199
Size: 6200
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4646 4678 4646
Size: 32 6168
PpuMI rG'GwC_Cy
Cuts at: 987 987
Size: 6200
PshAI GACnn'nnGTC
Cuts at: 2840 2840
Size: 6200
PsiI TTA'TAA
Cuts at: 1217 1264 1385 1469 1553 1637 2561 2813 1217
Size: 47 121 84 84 84 924 252 4604
Fragments arranged by size:
4604 924 252 121 84 84 84 47
PspOMI G'GGCC_C
Cuts at: 810 810
Size: 6200
PstI C_TGCA'G
Cuts at: 470 3247 3932 470
Size: 2777 685 2738
Fragments arranged by size:
2777 2738 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6200
PvuII CAG'CTG
Cuts at: 595 3625 3985 595
Size: 3030 360 2810
Fragments arranged by size:
3030 2810 360
RsrII CG'GwC_CG
Cuts at: 4395 4395
Size: 6200
SacI G_AGCT'C
Cuts at: 3214 3214
Size: 6200
SalI G'TCGA_C
Cuts at: 3237 3237
Size: 6200
SapI GCTCTTCn'nnn_
Cuts at: 1669 2089 2406 4223 4433 1669
Size: 420 317 1817 210 3436
Fragments arranged by size:
3436 1817 420 317 210
SbfI CC_TGCA'GG
Cuts at: 3247 3247
Size: 6200
ScaI AGT'ACT
Cuts at: 5717 5717
Size: 6200
SfcI C'TryA_G
Cuts at: 33 466 1497 3243 3928 4896 5087 33
Size: 433 1031 1746 685 968 191 1146
Fragments arranged by size:
1746 1146 1031 968 685 433 191
SfoI GGC'GCC
Cuts at: 1531 3632 3880 1531
Size: 2101 248 3851
Fragments arranged by size:
3851 2101 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6200
SgrAI Cr'CCGG_yG
Cuts at: 311 311
Size: 6200
SmaI CCC'GGG
Cuts at: 3222 3222
Size: 6200
SmlI C'TyrA_G
Cuts at: 3355 4270 4984 5246 3355
Size: 915 714 262 4309
Fragments arranged by size:
4309 915 714 262
SnaBI TAC'GTA
Cuts at: 2675 2675
Size: 6200
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6200
SphI G_CATG'C
Cuts at: 3253 3753 3253
Size: 500 5700
StuI AGG'CCT
Cuts at: 1511 1511
Size: 6200
StyI C'CwwG_G
Cuts at: 198 338 3334 3688 198
Size: 140 2996 354 2710
Fragments arranged by size:
2996 2710 354 140
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 907 2972 3783 3916 4375 4584 907
Size: 2065 811 133 459 209 2523
Fragments arranged by size:
2523 2065 811 459 209 133
TatI w'GTAC_w
Cuts at: 570 830 2358 2862 3030 5715 570
Size: 260 1528 504 168 2685 1055
Fragments arranged by size:
2685 1528 1055 504 260 168
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 713 745 5717 5749 713
Size: 32 4972 32 1164
Fragments arranged by size:
4972 1164 32 32
Tth111I GACn'n_nGTC
Cuts at: 3997 3997
Size: 6200
XbaI T'CTAG_A
Cuts at: 3231 3231
Size: 6200
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 265 3695 265
Size: 3430 2770
XmaI C'CCGG_G
Cuts at: 3220 3220
Size: 6200
XmnI GAAnn'nnTTC
Cuts at: 1576 1660 1912 2332 2584 1576
Size: 84 252 420 252 5192
Fragments arranged by size:
5192 420 252 252 84
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AleI AloI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BfrBI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI BtgZI
BtsI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NdeI NgoMIV NsiI NspI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AlfI AscI
AvrII BaeI BcgI BglI BmgBI BmtI BsiWI BsmBI
BspEI BssHII BstEII BstZ17I Bsu36I BtgI ClaI DraIII
EcoRV FseI FspAI HpaI MfeI NcoI NheI NotI
NruI PacI PasI PmlI PspXI PsrI SacII SanDI
SexAI SfiI SrfI SspI SwaI XhoI ZraI