Restriction map for
pF1KB3650
(Circular) (Six-base) MAPSORT of: pF1KB3650.seq Check: 3206 from: 1 to: 4547
pF1KB3650 4547 bp
With 182 enzymes: SgfI *
April 24, 2009 13:02 ..
AccI GT'mk_AC
Cuts at: 920 1585 920
Size: 665 3882
Acc65I G'GTAC_C
Cuts at: 680 1090 1563 680
Size: 410 473 3664
Fragments arranged by size:
3664 473 410
AclI AA'CG_TT
Cuts at: 4387 4387
Size: 4547
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2372 2804 3551 3819 2372
Size: 432 747 268 3100
Fragments arranged by size:
3100 747 432 268
AflIII A'CryG_T
Cuts at: 1899 3003 3980 1899
Size: 1104 977 2466
Fragments arranged by size:
2466 1104 977
AgeI A'CCGG_T
Cuts at: 2935 2935
Size: 4547
AhdI GACnn_n'nnGTC
Cuts at: 171 171
Size: 4547
AleI CACnn'nnGTG
Cuts at: 1265 1265
Size: 4547
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 940 972 940
Size: 32 4515
AlwNI CAG_nnn'CTG
Cuts at: 1531 1852 3419 1531
Size: 321 1567 2659
Fragments arranged by size:
2659 1567 321
ApaI G_GGCC'C
Cuts at: 394 721 394
Size: 327 4220
ApaLI G'TGCA_C
Cuts at: 3317 3317
Size: 4547
ApoI r'AATT_y
Cuts at: 1551 3900 4156 1551
Size: 2349 256 1942
Fragments arranged by size:
2349 1942 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4547
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4547
AvaI C'yCGr_G
Cuts at: 1567 1567
Size: 4547
BamHI G'GATC_C
Cuts at: 1572 1572
Size: 4547
BanI G'GyrC_C
Cuts at: 111 490 680 1090 1185 1563 1977 2225 2260
Size: 379 190 410 95 378 414 248 35
Cuts at: 2260 111
Size: 2398
Fragments arranged by size:
2398 414 410 379 378 248 190 95
35
BanII G_rGCy'C
Cuts at: 236 394 721 1561 2591 236
Size: 158 327 840 1030 2192
Fragments arranged by size:
2192 1030 840 327 158
BbeI G_GCGC'C
Cuts at: 494 1981 2229 494
Size: 1487 248 2812
Fragments arranged by size:
2812 1487 248
BbsI GAAGACnn'nnnn_
Cuts at: 766 1407 766
Size: 641 3906
BbvCI CC'TCA_GC
Cuts at: 548 548
Size: 4547
BciVI GTATCCnnnnn_n'
Cuts at: 2445 3206 4547 2445
Size: 761 1341 2445
Fragments arranged by size:
2445 1341 761
BclI T'GATC_A
Cuts at: 2067 2067
Size: 4547
BglI GCCn_nnn'nGGC
Cuts at: 145 145
Size: 4547
BglII A'GATC_T
Cuts at: 2062 4099 2062
Size: 2037 2510
BlpI GC'TnA_GC
Cuts at: 1659 1659
Size: 4547
Bme1580I G_kGCm'C
Cuts at: 394 721 2172 2265 3321 394
Size: 327 1451 93 1056 1620
Fragments arranged by size:
1620 1451 1056 327 93
BmrI ACTGGGnnnn_n'
Cuts at: 414 954 965 1098 1759 1840 2175 4319 414
Size: 540 11 133 661 81 335 2144 642
Fragments arranged by size:
2144 661 642 540 335 133 81 11
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 255 2914 255
Size: 2659 1888
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 397 1308 1448 397
Size: 911 140 3496
Fragments arranged by size:
3496 911 140
Bpu10I CC'TnA_GC
Cuts at: 437 548 437
Size: 111 4436
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 501 592 1723 2602 3094 3392 501
Size: 91 1131 879 492 298 1656
Fragments arranged by size:
1656 1131 879 492 298 91
BsaI GGTCTCn'nnnn_
Cuts at: 114 520 537 4251 114
Size: 406 17 3714 410
Fragments arranged by size:
3714 410 406 17
BsaAI yAC'GTr
Cuts at: 1307 1900 1307
Size: 593 3954
BsaBI GATnn'nnATC
Cuts at: 6 2061 4098 6
Size: 2055 2037 455
Fragments arranged by size:
2055 2037 455
BsaHI Gr'CG_yC
Cuts at: 491 593 1978 2226 2928 4424 491
Size: 102 1385 248 702 1496 614
Fragments arranged by size:
1496 1385 702 614 248 102
BsaWI w'CCGG_w
Cuts at: 1736 2257 2935 3209 3356 3767 1736
Size: 521 678 274 147 411 2516
Fragments arranged by size:
2516 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 313 343 556 586 940 970 1267 1297 1480
Size: 30 213 30 354 30 297 30 183
Cuts at: 1480 1510 313
Size: 30 3350
Fragments arranged by size:
3350 354 297 213 183 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 367 577 580 994 1345 367
Size: 210 3 414 351 3569
Fragments arranged by size:
3569 414 351 210 3
BseYI C'CCAG_C
Cuts at: 555 1043 1515 1524 1972 2730 3307 3803 555
Size: 488 472 9 448 758 577 496 1299
Fragments arranged by size:
1299 758 577 496 488 472 448 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 174 243 594 174
Size: 69 351 4127
Fragments arranged by size:
4127 351 69
BsiEI CG_ry'CG
Cuts at: 104 2089 2135 3343 104
Size: 1985 46 1208 1308
Fragments arranged by size:
1985 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 236 1561 2339 2529 3321 236
Size: 1325 778 190 792 1462
Fragments arranged by size:
1462 1325 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 236 394 721 1561 2172 2265 2339 2529 2591
Size: 158 327 840 611 93 74 190 62
Cuts at: 2591 3321 236
Size: 730 1462
Fragments arranged by size:
1462 840 730 611 327 190 158 93
74 62
BspEI T'CCGG_A
Cuts at: 3767 3767
Size: 4547
BspHI T'CATG_A
Cuts at: 3723 3723
Size: 4547
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1597 1811 2113 2494 1597
Size: 214 302 381 3650
Fragments arranged by size:
3650 381 302 214
BsrBI CCG'CTC
Cuts at: 50 915 2839 2893 4377 4542 50
Size: 865 1924 54 1484 165 55
Fragments arranged by size:
1924 1484 865 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2459 2459
Size: 4547
BsrFI r'CCGG_y
Cuts at: 1289 2545 2726 2935 1289
Size: 1256 181 209 2901
Fragments arranged by size:
2901 1256 209 181
BssSI C'ACGA_G
Cuts at: 2818 3176 2818
Size: 358 4189
BstAPI GCAn_nnn'nTGC
Cuts at: 154 1131 1924 154
Size: 977 793 2777
Fragments arranged by size:
2777 977 793
BstBI TT'CG_AA
Cuts at: 2908 2908
Size: 4547
BstXI CCAn_nnnn'nTGG
Cuts at: 1267 1267
Size: 4547
BstYI r'GATC_y
Cuts at: 1 1572 2039 2062 2397 2643 3655 3741 3753
Size: 1571 467 23 335 246 1012 86 12
Cuts at: 3753 4099 1
Size: 346 449
Fragments arranged by size:
1571 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 461 482 503 461
Size: 21 21 4505
Fragments arranged by size:
4505 21 21
BtgI C'CryG_G
Cuts at: 107 1154 1266 107
Size: 1047 112 3388
Fragments arranged by size:
3388 1047 112
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1210 1485 2497 2678 2841 1210
Size: 275 1012 181 163 2916
Fragments arranged by size:
2916 1012 275 181 163
BtsI GCAGTG_nn'
Cuts at: 582 674 1790 582
Size: 92 1116 3339
Fragments arranged by size:
3339 1116 92
ClaI AT'CG_AT
Cuts at: 725 725
Size: 4547
DraI TTT'AAA
Cuts at: 1546 1546
Size: 4547
DraIII CAC_nnn'GTG
Cuts at: 1131 1131
Size: 4547
DrdI GACnn_nn'nnGTC
Cuts at: 2253 3111 2253
Size: 858 3689
EaeI y'GGCC_r
Cuts at: 906 1161 1202 1269 1291 1533 2132 2306 2697
Size: 255 41 67 22 242 599 174 391
Cuts at: 2697 2724 4069 906
Size: 27 1345 1384
Fragments arranged by size:
1384 1345 599 391 255 242 174 67
41 27 22
EagI C'GGCC_G
Cuts at: 2132 2132
Size: 4547
EarI CTCTTCn'nnn_
Cuts at: 461 775 1072 2570 2780 461
Size: 314 297 1498 210 2228
Fragments arranged by size:
2228 1498 314 297 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2755 3065 3211 4357 2755
Size: 310 146 1146 2945
Fragments arranged by size:
2945 1146 310 146
EcoICRI GAG'CTC
Cuts at: 234 1559 234
Size: 1325 3222
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 397 1308 1448 2372 2804 3551 3819 397
Size: 911 140 924 432 747 268 1125
Fragments arranged by size:
1125 924 911 747 432 268 140
EcoNI CCTnn'n_nnAGG
Cuts at: 772 772
Size: 4547
EcoO109I rG'GnC_Cy
Cuts at: 390 391 471 717 718 1686 390
Size: 1 80 246 1 968 3251
Fragments arranged by size:
3251 968 246 80 1 1
EcoRI G'AATT_C
Cuts at: 1551 1551
Size: 4547
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2566 2566
Size: 4547
FspI TGC'GCA
Cuts at: 2328 2328
Size: 4547
HaeII r_GCGC'y
Cuts at: 494 795 1981 2229 3251 4230 494
Size: 301 1186 248 1022 979 811
Fragments arranged by size:
1186 1022 979 811 301 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 113 145 157 189 519 551 2389 2419 2421
Size: 32 12 32 330 32 1838 30 2
Cuts at: 2421 2451 113
Size: 30 2209
Fragments arranged by size:
2209 1838 330 32 32 32 30 30
12 2
HincII GTy'rAC
Cuts at: 1586 1586
Size: 4547
KasI G'GCGC_C
Cuts at: 490 1977 2225 490
Size: 1487 248 2812
Fragments arranged by size:
2812 1487 248
KpnI G_GTAC'C
Cuts at: 684 1094 1567 684
Size: 410 473 3664
Fragments arranged by size:
3664 473 410
MluI A'CGCG_T
Cuts at: 3980 3980
Size: 4547
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3218 3402 3218
Size: 184 4363
MscI TGG'CCA
Cuts at: 1204 1535 2308 1204
Size: 331 773 3443
Fragments arranged by size:
3443 773 331
MslI CAynn'nnrTG
Cuts at: 1265 2536 2663 1265
Size: 1271 127 3149
Fragments arranged by size:
3149 1271 127
MspA1I CmG'CkG
Cuts at: 188 1035 1043 1658 1972 2332 3345 3590 188
Size: 847 8 615 314 360 1013 245 1145
Fragments arranged by size:
1145 1013 847 615 360 314 245 8
NaeI GCC'GGC
Cuts at: 1291 2728 1291
Size: 1437 3110
NarI GG'CG_CC
Cuts at: 491 1978 2226 491
Size: 1487 248 2812
Fragments arranged by size:
2812 1487 248
NcoI C'CATG_G
Cuts at: 107 1154 107
Size: 1047 3500
NgoMIV G'CCGG_C
Cuts at: 1289 2726 1289
Size: 1437 3110
NspI r_CATG'y
Cuts at: 1600 2100 3007 1600
Size: 500 907 3140
Fragments arranged by size:
3140 907 500
PciI A'CATG_T
Cuts at: 3003 3003
Size: 4547
PflMI CCAn_nnn'nTGG
Cuts at: 1531 1531
Size: 4547
PfoI T'CCnGG_A
Cuts at: 809 857 809
Size: 48 4499
PmeI GTTT'AAAC
Cuts at: 1546 1546
Size: 4547
PmlI CAC'GTG
Cuts at: 1307 1307
Size: 4547
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 940 972 3686 3718 940
Size: 32 2714 32 1769
Fragments arranged by size:
2714 1769 32 32
PspOMI G'GGCC_C
Cuts at: 390 717 390
Size: 327 4220
PstI C_TGCA'G
Cuts at: 1594 2279 1594
Size: 685 3862
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4547
PvuII CAG'CTG
Cuts at: 1043 1972 2332 1043
Size: 929 360 3258
Fragments arranged by size:
3258 929 360
RsrII CG'GwC_CG
Cuts at: 2742 2742
Size: 4547
SacI G_AGCT'C
Cuts at: 236 1561 236
Size: 1325 3222
SalI G'TCGA_C
Cuts at: 1584 1584
Size: 4547
SapI GCTCTTCn'nnn_
Cuts at: 2570 2780 2570
Size: 210 4337
SbfI CC_TGCA'GG
Cuts at: 1594 1594
Size: 4547
ScaI AGT'ACT
Cuts at: 643 787 943 1438 1510 4064 643
Size: 144 156 495 72 2554 1126
Fragments arranged by size:
2554 1126 495 156 144 72
SfcI C'TryA_G
Cuts at: 33 900 1365 1590 2275 3268 3459 33
Size: 867 465 225 685 993 191 1121
Fragments arranged by size:
1121 993 867 685 465 225 191
SfoI GGC'GCC
Cuts at: 492 1979 2227 492
Size: 1487 248 2812
Fragments arranged by size:
2812 1487 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4547
SmaI CCC'GGG
Cuts at: 1569 1569
Size: 4547
SmlI C'TyrA_G
Cuts at: 516 607 1702 2617 3109 3371 516
Size: 91 1095 915 492 262 1692
Fragments arranged by size:
1692 1095 915 492 262 91
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4547
SphI G_CATG'C
Cuts at: 1600 2100 1600
Size: 500 4047
StuI AGG'CCT
Cuts at: 522 675 522
Size: 153 4394
StyI C'CwwG_G
Cuts at: 107 878 1154 1199 1681 2035 107
Size: 771 276 45 482 354 2619
Fragments arranged by size:
2619 771 482 354 276 45
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2130 2263 2722 2931 2130
Size: 133 459 209 3746
Fragments arranged by size:
3746 459 209 133
TatI w'GTAC_w
Cuts at: 329 641 785 941 1436 1508 4062 329
Size: 312 144 156 495 72 2554 814
Fragments arranged by size:
2554 814 495 312 156 144 72
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 202 234 556 588 4064 4096 202
Size: 32 322 32 3476 32 653
Fragments arranged by size:
3476 653 322 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2344 2344
Size: 4547
XbaI T'CTAG_A
Cuts at: 1578 1578
Size: 4547
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 901 2042 901
Size: 1141 3406
XmaI C'CCGG_G
Cuts at: 1567 1567
Size: 4547
XmnI GAAnn'nnTTC
Cuts at: 586 586
Size: 4547
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AleI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PflMI PfoI PmeI PmlI PpiI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AlfI AscI AvrII BaeI
BcgI BfrBI BmgBI BmtI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstZ17I CspCI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PpuMI PshAI PsiI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI
ZraI