Restriction map for
pF1KB3647
(Circular) (Six-base) MAPSORT of: pF1KB3647.seq Check: 8160 from: 1 to: 4907
pF1KB3647 4907 bp
With 182 enzymes: SgfI *
April 24, 2009 13:02 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 662 662
Size: 4907
AatII G_ACGT'C
Cuts at: 1538 1538
Size: 4907
AccI GT'mk_AC
Cuts at: 1154 1945 1154
Size: 791 4116
Acc65I G'GTAC_C
Cuts at: 1451 1923 1451
Size: 472 4435
AclI AA'CG_TT
Cuts at: 4747 4747
Size: 4907
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 319 446 679 724 2732 3164 3517 4179 319
Size: 127 233 45 2008 432 353 662 1047
Fragments arranged by size:
2008 1047 662 432 353 233 127 45
AfeI AGC'GCT
Cuts at: 1759 1759
Size: 4907
AflIII A'CryG_T
Cuts at: 2259 4059 4340 2259
Size: 1800 281 2826
Fragments arranged by size:
2826 1800 281
AleI CACnn'nnGTG
Cuts at: 1643 1643
Size: 4907
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1661 1661
Size: 4907
AlwNI CAG_nnn'CTG
Cuts at: 1722 2212 3650 1722
Size: 490 1438 2979
Fragments arranged by size:
2979 1438 490
ApaLI G'TGCA_C
Cuts at: 622 3745 622
Size: 3123 1784
ApoI r'AATT_y
Cuts at: 488 856 1440 1911 4260 4516 488
Size: 368 584 471 2349 256 879
Fragments arranged by size:
2349 879 584 471 368 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4907
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4907
AvaI C'yCGr_G
Cuts at: 742 1927 742
Size: 1185 3722
BamHI G'GATC_C
Cuts at: 1137 1932 1137
Size: 795 4112
BanI G'GyrC_C
Cuts at: 1451 1645 1923 2337 2585 2620 1451
Size: 194 278 414 248 35 3738
Fragments arranged by size:
3738 414 278 248 194 35
BanII G_rGCy'C
Cuts at: 368 542 1921 2951 368
Size: 174 1379 1030 2324
Fragments arranged by size:
2324 1379 1030 174
BbeI G_GCGC'C
Cuts at: 2341 2589 2341
Size: 248 4659
BbsI GAAGACnn'nnnn_
Cuts at: 1123 1123
Size: 4907
BbvCI CC'TCA_GC
Cuts at: 259 1313 259
Size: 1054 3853
BciVI GTATCCnnnnn_n'
Cuts at: 1592 2805 3861 4907 1592
Size: 1213 1056 1046 1592
Fragments arranged by size:
1592 1213 1056 1046
BclI T'GATC_A
Cuts at: 2427 2427
Size: 4907
BglI GCCn_nnn'nGGC
Cuts at: 250 1838 250
Size: 1588 3319
BglII A'GATC_T
Cuts at: 2422 4459 2422
Size: 2037 2870
BlpI GC'TnA_GC
Cuts at: 875 2019 875
Size: 1144 3763
Bme1580I G_kGCm'C
Cuts at: 626 1650 1801 2532 2625 3749 626
Size: 1024 151 731 93 1124 1784
Fragments arranged by size:
1784 1124 1024 731 151 93
BmrI ACTGGGnnnn_n'
Cuts at: 1186 2119 2200 2535 4679 1186
Size: 933 81 335 2144 1414
Fragments arranged by size:
2144 1414 933 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1316 3274 1316
Size: 1958 2949
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 337 1390 1579 1834 337
Size: 1053 189 255 3410
Fragments arranged by size:
3410 1053 255 189
Bpu10I CC'TnA_GC
Cuts at: 259 440 1313 259
Size: 181 873 3853
Fragments arranged by size:
3853 873 181
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 526 2083 2962 3676 3974 526
Size: 1557 879 714 298 1459
Fragments arranged by size:
1557 1459 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 1043 4611 1043
Size: 3568 1339
BsaAI yAC'GTr
Cuts at: 852 2260 852
Size: 1408 3499
BsaBI GATnn'nnATC
Cuts at: 6 571 2421 4458 6
Size: 565 1850 2037 455
Fragments arranged by size:
2037 1850 565 455
BsaHI Gr'CG_yC
Cuts at: 218 1043 1535 2338 2586 3288 4784 218
Size: 825 492 803 248 702 1496 341
Fragments arranged by size:
1496 825 803 702 492 341 248
BsaWI w'CCGG_w
Cuts at: 510 905 2096 2617 3295 3706 3853 4127 510
Size: 395 1191 521 678 411 147 274 1290
Fragments arranged by size:
1290 1191 678 521 411 395 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 529 559 991 1021 529
Size: 30 432 30 4415
Fragments arranged by size:
4415 432 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 712 733 1175 1657 712
Size: 21 442 482 3962
Fragments arranged by size:
3962 482 442 21
BseYI C'CCAG_C
Cuts at: 869 995 1074 1595 1715 2332 3090 3755 4163
Size: 126 79 521 120 617 758 665 408
Cuts at: 4163 869
Size: 1613
Fragments arranged by size:
1613 758 665 617 521 408 126 120
79
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 982 1621 1848 982
Size: 639 227 4041
Fragments arranged by size:
4041 639 227
BsiEI CG_ry'CG
Cuts at: 104 238 2449 2495 3725 104
Size: 134 2211 46 1230 1286
Fragments arranged by size:
2211 1286 1230 134 46
BsiHKAI G_wGCw'C
Cuts at: 176 542 626 1921 2699 2889 3749 176
Size: 366 84 1295 778 190 860 1334
Fragments arranged by size:
1334 1295 860 778 366 190 84
BsmI GAATG_Cn'
Cuts at: 1302 1302
Size: 4907
BsmBI CGTCTCn'nnnn_
Cuts at: 209 209
Size: 4907
Bsp1286I G_dGCh'C
Cuts at: 176 368 542 626 1650 1801 1921 2532 2625
Size: 192 174 84 1024 151 120 611 93
Cuts at: 2625 2699 2889 2951 3749 176
Size: 74 190 62 798 1334
Fragments arranged by size:
1334 1024 798 611 192 190 174 151
120 93 84 74 62
BspEI T'CCGG_A
Cuts at: 510 510
Size: 4907
BspHI T'CATG_A
Cuts at: 1787 3339 1787
Size: 1552 3355
BspMI ACCTGCnnnn'nnnn_
Cuts at: 566 662 816 1370 1957 2171 2473 2854 566
Size: 96 154 554 587 214 302 381 2619
Fragments arranged by size:
2619 587 554 381 302 214 154 96
BsrBI CCG'CTC
Cuts at: 50 1248 3199 3253 4737 4902 50
Size: 1198 1951 54 1484 165 55
Fragments arranged by size:
1951 1484 1198 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2819 2819
Size: 4907
BsrFI r'CCGG_y
Cuts at: 163 191 332 879 2905 3086 163
Size: 28 141 547 2026 181 1984
Fragments arranged by size:
2026 1984 547 181 141 28
BsrGI T'GTAC_A
Cuts at: 1013 1346 1751 1013
Size: 333 405 4169
Fragments arranged by size:
4169 405 333
BssHII G'CGCG_C
Cuts at: 240 263 240
Size: 23 4884
BssSI C'ACGA_G
Cuts at: 226 3178 3886 226
Size: 2952 708 1247
Fragments arranged by size:
2952 1247 708
BstAPI GCAn_nnn'nTGC
Cuts at: 2284 2284
Size: 4907
BstBI TT'CG_AA
Cuts at: 3268 3268
Size: 4907
BstXI CCAn_nnnn'nTGG
Cuts at: 1034 1034
Size: 4907
BstYI r'GATC_y
Cuts at: 1 230 1137 1932 2399 2422 2757 3003 3309
Size: 229 907 795 467 23 335 246 306
Cuts at: 3309 3321 3407 4459 1
Size: 12 86 1052 449
Fragments arranged by size:
1052 907 795 467 449 335 306 246
229 86 23 12
BstZ17I GTA'TAC
Cuts at: 1155 1155
Size: 4907
BtgI C'CryG_G
Cuts at: 107 984 1590 107
Size: 877 606 3424
Fragments arranged by size:
3424 877 606
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 183 2857 3038 3201 183
Size: 2674 181 163 1889
Fragments arranged by size:
2674 1889 181 163
BtsI GCAGTG_nn'
Cuts at: 2150 2150
Size: 4907
DraI TTT'AAA
Cuts at: 1430 1906 1430
Size: 476 4431
DraIII CAC_nnn'GTG
Cuts at: 530 530
Size: 4907
DrdI GACnn_nn'nnGTC
Cuts at: 2613 3957 2613
Size: 1344 3563
EaeI y'GGCC_r
Cuts at: 182 235 251 2492 2666 3057 3084 4429 182
Size: 53 16 2241 174 391 27 1345 660
Fragments arranged by size:
2241 1345 660 391 174 53 27 16
EagI C'GGCC_G
Cuts at: 235 2492 235
Size: 2257 2650
EarI CTCTTCn'nnn_
Cuts at: 117 181 689 766 1307 1404 2930 3140 117
Size: 64 508 77 541 97 1526 210 1884
Fragments arranged by size:
1884 1526 541 508 210 97 77 64
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3115 3857 4003 4717 3115
Size: 742 146 714 3305
Fragments arranged by size:
3305 742 714 146
EcoICRI GAG'CTC
Cuts at: 540 1919 540
Size: 1379 3528
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 319 337 446 679 724 1390 1579 1834 2732
Size: 18 109 233 45 666 189 255 898
Cuts at: 2732 3164 3517 4179 319
Size: 432 353 662 1047
Fragments arranged by size:
1047 898 666 662 432 353 255 233
189 109 45 18
EcoNI CCTnn'n_nnAGG
Cuts at: 658 658
Size: 4907
EcoO109I rG'GnC_Cy
Cuts at: 399 445 555 597 708 1070 1215 1830 2046
Size: 46 110 42 111 362 145 615 216
Cuts at: 2046 399
Size: 3260
Fragments arranged by size:
3260 615 362 216 145 111 110 46
42
EcoRI G'AATT_C
Cuts at: 856 1911 856
Size: 1055 3852
EcoRV GAT'ATC
Cuts at: 569 569
Size: 4907
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 418 1647 2926 418
Size: 1229 1279 2399
Fragments arranged by size:
2399 1279 1229
FspI TGC'GCA
Cuts at: 208 2688 208
Size: 2480 2427
HaeII r_GCGC'y
Cuts at: 275 306 1761 2341 2589 3819 4590 275
Size: 31 1455 580 248 1230 771 592
Fragments arranged by size:
1455 1230 771 592 580 248 31
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 586 618 777 809 1321 1353 2749 2779 2781
Size: 32 159 32 512 32 1396 30 2
Cuts at: 2781 2811 586
Size: 30 2682
Fragments arranged by size:
2682 1396 512 159 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1104 1946 1104
Size: 842 4065
KasI G'GCGC_C
Cuts at: 2337 2585 2337
Size: 248 4659
KpnI G_GTAC'C
Cuts at: 1455 1927 1455
Size: 472 4435
MluI A'CGCG_T
Cuts at: 4340 4340
Size: 4907
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3666 3850 3666
Size: 184 4723
MscI TGG'CCA
Cuts at: 184 253 2668 184
Size: 69 2415 2423
Fragments arranged by size:
2423 2415 69
MslI CAynn'nnrTG
Cuts at: 202 586 673 1643 2896 3023 202
Size: 384 87 970 1253 127 2086
Fragments arranged by size:
2086 1253 970 384 127 87
MspA1I CmG'CkG
Cuts at: 212 339 865 966 995 1368 1557 1682 1862
Size: 127 526 101 29 373 189 125 180
Cuts at: 1862 2018 2332 2692 3476 3721 212
Size: 156 314 360 784 245 1398
Fragments arranged by size:
1398 784 526 373 360 314 245 189
180 156 127 125 101 29
NaeI GCC'GGC
Cuts at: 165 193 3088 165
Size: 28 2895 1984
Fragments arranged by size:
2895 1984 28
NarI GG'CG_CC
Cuts at: 2338 2586 2338
Size: 248 4659
NcoI C'CATG_G
Cuts at: 107 1590 107
Size: 1483 3424
NgoMIV G'CCGG_C
Cuts at: 163 191 3086 163
Size: 28 2895 1984
Fragments arranged by size:
2895 1984 28
NspI r_CATG'y
Cuts at: 1014 1960 2460 4063 1014
Size: 946 500 1603 1858
Fragments arranged by size:
1858 1603 946 500
PasI CC'CwG_GG
Cuts at: 1650 1650
Size: 4907
PciI A'CATG_T
Cuts at: 4059 4059
Size: 4907
PflMI CCAn_nnn'nTGG
Cuts at: 1722 1722
Size: 4907
PfoI T'CCnGG_A
Cuts at: 284 896 1377 1721 284
Size: 612 481 344 3470
Fragments arranged by size:
3470 612 481 344
PmeI GTTT'AAAC
Cuts at: 1906 1906
Size: 4907
PmlI CAC'GTG
Cuts at: 852 852
Size: 4907
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3353 3385 3353
Size: 32 4875
PpuMI rG'GwC_Cy
Cuts at: 445 555 708 1070 1215 445
Size: 110 153 362 145 4137
Fragments arranged by size:
4137 362 153 145 110
PstI C_TGCA'G
Cuts at: 1954 2639 1954
Size: 685 4222
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4907
PvuII CAG'CTG
Cuts at: 339 995 1862 2332 2692 339
Size: 656 867 470 360 2554
Fragments arranged by size:
2554 867 656 470 360
RsrII CG'GwC_CG
Cuts at: 617 3102 617
Size: 2485 2422
SacI G_AGCT'C
Cuts at: 542 1921 542
Size: 1379 3528
SalI G'TCGA_C
Cuts at: 1944 1944
Size: 4907
SanDI GG'GwC_CC
Cuts at: 445 445
Size: 4907
SapI GCTCTTCn'nnn_
Cuts at: 181 1307 1404 2930 3140 181
Size: 1126 97 1526 210 1948
Fragments arranged by size:
1948 1526 1126 210 97
SbfI CC_TGCA'GG
Cuts at: 1954 1954
Size: 4907
ScaI AGT'ACT
Cuts at: 547 4424 547
Size: 3877 1030
SfcI C'TryA_G
Cuts at: 33 1950 2635 3603 3794 33
Size: 1917 685 968 191 1146
Fragments arranged by size:
1917 1146 968 685 191
SfoI GGC'GCC
Cuts at: 2339 2587 2339
Size: 248 4659
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4907
SmaI CCC'GGG
Cuts at: 744 1929 744
Size: 1185 3722
SmlI C'TyrA_G
Cuts at: 541 2062 2977 3691 3953 541
Size: 1521 915 714 262 1495
Fragments arranged by size:
1521 1495 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4907
SphI G_CATG'C
Cuts at: 1960 2460 1960
Size: 500 4407
SspI AAT'ATT
Cuts at: 1170 1577 1170
Size: 407 4500
StuI AGG'CCT
Cuts at: 782 782
Size: 4907
StyI C'CwwG_G
Cuts at: 107 360 551 1590 1622 2041 2395 107
Size: 253 191 1039 32 419 354 2619
Fragments arranged by size:
2619 1039 419 354 253 191 32
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1633 2490 2623 3082 3291 1633
Size: 857 133 459 209 3249
Fragments arranged by size:
3249 857 459 209 133
TatI w'GTAC_w
Cuts at: 545 1013 1346 1751 4422 545
Size: 468 333 405 2671 1030
Fragments arranged by size:
2671 1030 468 405 333
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 991 1023 4424 4456 991
Size: 32 3401 32 1442
Fragments arranged by size:
3401 1442 32 32
Tth111I GACn'n_nGTC
Cuts at: 1735 2704 1735
Size: 969 3938
XbaI T'CTAG_A
Cuts at: 1938 1938
Size: 4907
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 457 1034 2402 457
Size: 577 1368 2962
Fragments arranged by size:
2962 1368 577
XmaI C'CCGG_G
Cuts at: 742 1927 742
Size: 1185 3722
XmnI GAAnn'nnTTC
Cuts at: 668 1114 668
Size: 446 4461
ZraI GAC'GTC
Cuts at: 1536 1536
Size: 4907
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstXI BstYI BstZ17I BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI ZraI
Enzymes that do not cut:
AflII AgeI AhdI AloI ApaI AscI AvrII BaeI
BcgI BfrBI BmgBI BmtI BsiWI BstEII Bsu36I ClaI
CspCI FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PshAI PsiI PspOMI PspXI
PsrI SacII SexAI SfiI SgrAI SnaBI SrfI SwaI
XhoI