Restriction map for
pF1KB3644
(Circular) (Six-base) MAPSORT of: pF1KB3644.seq Check: 5308 from: 1 to: 4250
pF1KB3644 4250 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1053 1053
Size: 4250
AatII G_ACGT'C
Cuts at: 115 115
Size: 4250
AccI GT'mk_AC
Cuts at: 827 1288 827
Size: 461 3789
Acc65I G'GTAC_C
Cuts at: 595 1266 595
Size: 671 3579
AclI AA'CG_TT
Cuts at: 4090 4090
Size: 4250
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 745 2075 2507 2860 3522 745
Size: 1330 432 353 662 1473
Fragments arranged by size:
1473 1330 662 432 353
AfeI AGC'GCT
Cuts at: 250 250
Size: 4250
AflIII A'CryG_T
Cuts at: 1602 3402 3683 1602
Size: 1800 281 2169
Fragments arranged by size:
2169 1800 281
AlwNI CAG_nnn'CTG
Cuts at: 838 1555 2993 838
Size: 717 1438 2095
Fragments arranged by size:
2095 1438 717
ApaI G_GGCC'C
Cuts at: 175 175
Size: 4250
ApaLI G'TGCA_C
Cuts at: 442 3088 442
Size: 2646 1604
ApoI r'AATT_y
Cuts at: 1254 3603 3859 1254
Size: 2349 256 1645
Fragments arranged by size:
2349 1645 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4250
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4250
AvaI C'yCGr_G
Cuts at: 1270 1270
Size: 4250
BamHI G'GATC_C
Cuts at: 1275 1275
Size: 4250
BanI G'GyrC_C
Cuts at: 595 1266 1680 1928 1963 595
Size: 671 414 248 35 2882
Fragments arranged by size:
2882 671 414 248 35
BanII G_rGCy'C
Cuts at: 175 1264 2294 175
Size: 1089 1030 2131
Fragments arranged by size:
2131 1089 1030
BbeI G_GCGC'C
Cuts at: 1684 1932 1684
Size: 248 4002
BciVI GTATCCnnnnn_n'
Cuts at: 173 2148 3204 4250 173
Size: 1975 1056 1046 173
Fragments arranged by size:
1975 1056 1046 173
BclI T'GATC_A
Cuts at: 1770 1770
Size: 4250
BglII A'GATC_T
Cuts at: 906 1765 3802 906
Size: 859 2037 1354
Fragments arranged by size:
2037 1354 859
BlpI GC'TnA_GC
Cuts at: 1362 1362
Size: 4250
Bme1580I G_kGCm'C
Cuts at: 175 446 1221 1875 1968 3092 175
Size: 271 775 654 93 1124 1333
Fragments arranged by size:
1333 1124 775 654 271 93
BmrI ACTGGGnnnn_n'
Cuts at: 1462 1543 1878 4022 1462
Size: 81 335 2144 1690
Fragments arranged by size:
2144 1690 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 481 2617 481
Size: 2136 2114
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 418 476 418
Size: 58 4192
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 247 1156 1426 2305 3019 3317 247
Size: 909 270 879 714 298 1180
Fragments arranged by size:
1180 909 879 714 298 270
BsaI GGTCTCn'nnnn_
Cuts at: 631 883 1151 3954 631
Size: 252 268 2803 927
Fragments arranged by size:
2803 927 268 252
BsaAI yAC'GTr
Cuts at: 1603 1603
Size: 4250
BsaBI GATnn'nnATC
Cuts at: 6 1764 3801 6
Size: 1758 2037 455
Fragments arranged by size:
2037 1758 455
BsaHI Gr'CG_yC
Cuts at: 112 1681 1929 2631 4127 112
Size: 1569 248 702 1496 235
Fragments arranged by size:
1569 1496 702 248 235
BsaWI w'CCGG_w
Cuts at: 1439 1960 2638 3049 3196 3470 1439
Size: 521 678 411 147 274 2219
Fragments arranged by size:
2219 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 718 1030 1195 718
Size: 312 165 3773
Fragments arranged by size:
3773 312 165
BseYI C'CCAG_C
Cuts at: 554 1675 2433 3098 3506 554
Size: 1121 758 665 408 1298
Fragments arranged by size:
1298 1121 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 1792 1838 3068 104
Size: 1688 46 1230 1286
Fragments arranged by size:
1688 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 236 446 566 1264 2042 2232 3092 236
Size: 210 120 698 778 190 860 1394
Fragments arranged by size:
1394 860 778 698 210 190 120
Bsp1286I G_dGCh'C
Cuts at: 175 236 446 566 1221 1264 1875 1968 2042
Size: 61 210 120 655 43 611 93 74
Cuts at: 2042 2232 2294 3092 175
Size: 190 62 798 1333
Fragments arranged by size:
1333 798 655 611 210 190 120 93
74 62 61 43
BspHI T'CATG_A
Cuts at: 695 2682 695
Size: 1987 2263
BspMI ACCTGCnnnn'nnnn_
Cuts at: 401 1016 1053 1300 1514 1816 2197 401
Size: 615 37 247 214 302 381 2454
Fragments arranged by size:
2454 615 381 302 247 214 37
BsrBI CCG'CTC
Cuts at: 50 2542 2596 4080 4245 50
Size: 2492 54 1484 165 55
Fragments arranged by size:
2492 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 236 1245 2162 236
Size: 1009 917 2324
Fragments arranged by size:
2324 1009 917
BsrFI r'CCGG_y
Cuts at: 158 2248 2429 158
Size: 2090 181 1979
Fragments arranged by size:
2090 1979 181
BssSI C'ACGA_G
Cuts at: 2521 3229 2521
Size: 708 3542
BstAPI GCAn_nnn'nTGC
Cuts at: 689 1627 689
Size: 938 3312
BstBI TT'CG_AA
Cuts at: 2611 2611
Size: 4250
BstXI CCAn_nnnn'nTGG
Cuts at: 576 576
Size: 4250
BstYI r'GATC_y
Cuts at: 1 906 1019 1275 1742 1765 2100 2346 2652
Size: 905 113 256 467 23 335 246 306
Cuts at: 2652 2664 2750 3802 1
Size: 12 86 1052 449
Fragments arranged by size:
1052 905 467 449 335 306 256 246
113 86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2200 2381 2544 2200
Size: 181 163 3906
Fragments arranged by size:
3906 181 163
BtsI GCAGTG_nn'
Cuts at: 301 927 1221 1493 301
Size: 626 294 272 3058
Fragments arranged by size:
3058 626 294 272
DraI TTT'AAA
Cuts at: 1249 1249
Size: 4250
DrdI GACnn_nn'nnGTC
Cuts at: 969 1956 3300 969
Size: 987 1344 1919
Fragments arranged by size:
1919 1344 987
EaeI y'GGCC_r
Cuts at: 413 533 1835 2009 2400 2427 3772 413
Size: 120 1302 174 391 27 1345 891
Fragments arranged by size:
1345 1302 891 391 174 120 27
EagI C'GGCC_G
Cuts at: 1835 1835
Size: 4250
EarI CTCTTCn'nnn_
Cuts at: 322 472 920 2273 2483 322
Size: 150 448 1353 210 2089
Fragments arranged by size:
2089 1353 448 210 150
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 204 2458 3200 3346 4060 204
Size: 2254 742 146 714 394
Fragments arranged by size:
2254 742 714 394 146
EcoICRI GAG'CTC
Cuts at: 1262 1262
Size: 4250
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 418 476 745 2075 2507 2860 3522 418
Size: 58 269 1330 432 353 662 1146
Fragments arranged by size:
1330 1146 662 432 353 269 58
EcoNI CCTnn'n_nnAGG
Cuts at: 1012 1012
Size: 4250
EcoO109I rG'GnC_Cy
Cuts at: 267 390 1389 267
Size: 123 999 3128
Fragments arranged by size:
3128 999 123
EcoRI G'AATT_C
Cuts at: 1254 1254
Size: 4250
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2269 2269
Size: 4250
FspI TGC'GCA
Cuts at: 718 997 1231 2031 718
Size: 279 234 800 2937
Fragments arranged by size:
2937 800 279 234
FspAI rTGC'GCAy
Cuts at: 997 1231 997
Size: 234 4016
HaeII r_GCGC'y
Cuts at: 252 303 1684 1932 3162 3933 252
Size: 51 1381 248 1230 771 569
Fragments arranged by size:
1381 1230 771 569 248 51
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 820 852 2092 2122 2124 2154 820
Size: 32 1240 30 2 30 2916
Fragments arranged by size:
2916 1240 32 30 30 2
HincII GTy'rAC
Cuts at: 1289 1289
Size: 4250
HindIII A'AGCT_T
Cuts at: 307 307
Size: 4250
KasI G'GCGC_C
Cuts at: 1680 1928 1680
Size: 248 4002
KpnI G_GTAC'C
Cuts at: 599 1270 599
Size: 671 3579
MluI A'CGCG_T
Cuts at: 3683 3683
Size: 4250
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1157 3009 3193 1157
Size: 1852 184 2214
Fragments arranged by size:
2214 1852 184
MscI TGG'CCA
Cuts at: 535 2011 535
Size: 1476 2774
MslI CAynn'nnrTG
Cuts at: 289 2239 2366 289
Size: 1950 127 2173
Fragments arranged by size:
2173 1950 127
MspA1I CmG'CkG
Cuts at: 558 1361 1675 2035 2819 3064 558
Size: 803 314 360 784 245 1744
Fragments arranged by size:
1744 803 784 360 314 245
NaeI GCC'GGC
Cuts at: 160 2431 160
Size: 2271 1979
NarI GG'CG_CC
Cuts at: 1681 1929 1681
Size: 248 4002
NgoMIV G'CCGG_C
Cuts at: 158 2429 158
Size: 2271 1979
NspI r_CATG'y
Cuts at: 294 661 1303 1803 3406 294
Size: 367 642 500 1603 1138
Fragments arranged by size:
1603 1138 642 500 367
PasI CC'CwG_GG
Cuts at: 175 175
Size: 4250
PciI A'CATG_T
Cuts at: 3402 3402
Size: 4250
PfoI T'CCnGG_A
Cuts at: 575 575
Size: 4250
PmeI GTTT'AAAC
Cuts at: 1249 1249
Size: 4250
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2696 2728 2696
Size: 32 4218
PpuMI rG'GwC_Cy
Cuts at: 267 390 267
Size: 123 4127
PspOMI G'GGCC_C
Cuts at: 171 171
Size: 4250
PstI C_TGCA'G
Cuts at: 1297 1982 1297
Size: 685 3565
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4250
PvuII CAG'CTG
Cuts at: 558 1675 2035 558
Size: 1117 360 2773
Fragments arranged by size:
2773 1117 360
RsrII CG'GwC_CG
Cuts at: 2445 2445
Size: 4250
SacI G_AGCT'C
Cuts at: 1264 1264
Size: 4250
SalI G'TCGA_C
Cuts at: 1287 1287
Size: 4250
SapI GCTCTTCn'nnn_
Cuts at: 322 2273 2483 322
Size: 1951 210 2089
Fragments arranged by size:
2089 1951 210
SbfI CC_TGCA'GG
Cuts at: 1297 1297
Size: 4250
ScaI AGT'ACT
Cuts at: 3767 3767
Size: 4250
SexAI A'CCwGG_T
Cuts at: 803 803
Size: 4250
SfcI C'TryA_G
Cuts at: 33 1293 1978 2946 3137 33
Size: 1260 685 968 191 1146
Fragments arranged by size:
1260 1146 968 685 191
SfoI GGC'GCC
Cuts at: 1682 1930 1682
Size: 248 4002
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4250
SmaI CCC'GGG
Cuts at: 1272 1272
Size: 4250
SmlI C'TyrA_G
Cuts at: 262 1171 1405 2320 3034 3296 262
Size: 909 234 915 714 262 1216
Fragments arranged by size:
1216 915 909 714 262 234
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4250
SphI G_CATG'C
Cuts at: 661 1303 1803 661
Size: 642 500 3108
Fragments arranged by size:
3108 642 500
StyI C'CwwG_G
Cuts at: 122 776 1384 1738 122
Size: 654 608 354 2634
Fragments arranged by size:
2634 654 608 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1833 1966 2425 2634 1833
Size: 133 459 209 3449
Fragments arranged by size:
3449 459 209 133
TatI w'GTAC_w
Cuts at: 3765 3765
Size: 4250
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3767 3799 3767
Size: 32 4218
Tth111I GACn'n_nGTC
Cuts at: 2047 2047
Size: 4250
XbaI T'CTAG_A
Cuts at: 1281 1281
Size: 4250
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 490 1745 490
Size: 1255 2995
XmaI C'CCGG_G
Cuts at: 1270 1270
Size: 4250
ZraI GAC'GTC
Cuts at: 113 113
Size: 4250
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PasI
PciI PfoI PmeI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AflII AgeI AhdI AleI AlfI AloI AscI AvrII
BaeI BbsI BbvCI BcgI BfrBI BglI BmgBI BmtI
Bpu10I BsaXI BsgI BsiWI BsmI BsmBI BspEI BsrGI
BssHII BstEII BstZ17I Bsu36I BtgI ClaI CspCI DraIII
EcoRV FseI HpaI MfeI NcoI NdeI NheI NotI
NruI NsiI PacI PflMI PmlI PshAI PsiI PspXI
PsrI SacII SanDI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI