Restriction map for
pF1KB3634
(Circular) (Six-base) MAPSORT of: pF1KB3634.seq Check: 9935 from: 1 to: 4112
pF1KB3634 4112 bp
With 182 enzymes: SgfI *
April 24, 2009 13:02 ..
AccI GT'mk_AC
Cuts at: 1150 1150
Size: 4112
Acc65I G'GTAC_C
Cuts at: 1128 1128
Size: 4112
AclI AA'CG_TT
Cuts at: 3952 3952
Size: 4112
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1937 2369 3116 3384 1937
Size: 432 747 268 2665
Fragments arranged by size:
2665 747 432 268
AflIII A'CryG_T
Cuts at: 1464 2568 3545 1464
Size: 1104 977 2031
Fragments arranged by size:
2031 1104 977
AgeI A'CCGG_T
Cuts at: 2500 2500
Size: 4112
AleI CACnn'nnGTG
Cuts at: 431 538 431
Size: 107 4005
AlwNI CAG_nnn'CTG
Cuts at: 1417 2984 1417
Size: 1567 2545
ApaLI G'TGCA_C
Cuts at: 2882 2882
Size: 4112
ApoI r'AATT_y
Cuts at: 1116 3465 3721 1116
Size: 2349 256 1507
Fragments arranged by size:
2349 1507 256
AseI AT'TA_AT
Cuts at: 20 1004 20
Size: 984 3128
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4112
AvaI C'yCGr_G
Cuts at: 1132 1132
Size: 4112
BamHI G'GATC_C
Cuts at: 1137 1137
Size: 4112
BanI G'GyrC_C
Cuts at: 748 1128 1542 1790 1825 748
Size: 380 414 248 35 3035
Fragments arranged by size:
3035 414 380 248 35
BanII G_rGCy'C
Cuts at: 1126 2156 1126
Size: 1030 3082
BbeI G_GCGC'C
Cuts at: 1546 1794 1546
Size: 248 3864
BciVI GTATCCnnnnn_n'
Cuts at: 2010 2771 4112 2010
Size: 761 1341 2010
Fragments arranged by size:
2010 1341 761
BclI T'GATC_A
Cuts at: 1632 1632
Size: 4112
BglII A'GATC_T
Cuts at: 303 1627 3664 303
Size: 1324 2037 751
Fragments arranged by size:
2037 1324 751
BlpI GC'TnA_GC
Cuts at: 185 344 1224 185
Size: 159 880 3073
Fragments arranged by size:
3073 880 159
Bme1580I G_kGCm'C
Cuts at: 1737 1830 2886 1737
Size: 93 1056 2963
Fragments arranged by size:
2963 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1324 1405 1740 3884 1324
Size: 81 335 2144 1552
Fragments arranged by size:
2144 1552 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2479 2479
Size: 4112
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 458 971 458
Size: 513 3599
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 508 1288 2167 2659 2957 508
Size: 780 879 492 298 1663
Fragments arranged by size:
1663 879 780 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3816 3816
Size: 4112
BsaAI yAC'GTr
Cuts at: 1465 1465
Size: 4112
BsaBI GATnn'nnATC
Cuts at: 6 1626 3663 6
Size: 1620 2037 455
Fragments arranged by size:
2037 1620 455
BsaHI Gr'CG_yC
Cuts at: 1543 1791 2493 3989 1543
Size: 248 702 1496 1666
Fragments arranged by size:
1666 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1301 1822 2500 2774 2921 3332 1301
Size: 521 678 274 147 411 2081
Fragments arranged by size:
2081 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 242 272 242
Size: 30 4082
BseYI C'CCAG_C
Cuts at: 970 1537 2295 2872 3368 970
Size: 567 758 577 496 1714
Fragments arranged by size:
1714 758 577 567 496
BsiEI CG_ry'CG
Cuts at: 104 1654 1700 2908 104
Size: 1550 46 1208 1308
Fragments arranged by size:
1550 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1126 1904 2094 2886 1126
Size: 778 190 792 2352
Fragments arranged by size:
2352 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 1126 1737 1830 1904 2094 2156 2886 1126
Size: 611 93 74 190 62 730 2352
Fragments arranged by size:
2352 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3332 3332
Size: 4112
BspHI T'CATG_A
Cuts at: 3288 3288
Size: 4112
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1162 1376 1678 2059 1162
Size: 214 302 381 3215
Fragments arranged by size:
3215 381 302 214
BsrBI CCG'CTC
Cuts at: 50 118 2404 2458 3942 4107 50
Size: 68 2286 54 1484 165 55
Fragments arranged by size:
2286 1484 165 68 55 54
BsrDI GCAATG_nn'
Cuts at: 151 512 2024 151
Size: 361 1512 2239
Fragments arranged by size:
2239 1512 361
BsrFI r'CCGG_y
Cuts at: 2110 2291 2500 2110
Size: 181 209 3722
Fragments arranged by size:
3722 209 181
BsrGI T'GTAC_A
Cuts at: 356 571 356
Size: 215 3897
BssSI C'ACGA_G
Cuts at: 2383 2741 2383
Size: 358 3754
BstAPI GCAn_nnn'nTGC
Cuts at: 562 844 1489 562
Size: 282 645 3185
Fragments arranged by size:
3185 645 282
BstBI TT'CG_AA
Cuts at: 2473 2473
Size: 4112
BstXI CCAn_nnnn'nTGG
Cuts at: 759 759
Size: 4112
BstYI r'GATC_y
Cuts at: 1 303 1137 1604 1627 1962 2208 3220 3306
Size: 302 834 467 23 335 246 1012 86
Cuts at: 3306 3318 3664 1
Size: 12 346 449
Fragments arranged by size:
1012 834 467 449 346 335 302 246
86 23 12
BtgI C'CryG_G
Cuts at: 612 612
Size: 4112
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2062 2243 2406 2062
Size: 181 163 3768
Fragments arranged by size:
3768 181 163
BtsI GCAGTG_nn'
Cuts at: 1355 1355
Size: 4112
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 228 263 228
Size: 35 4077
DraI TTT'AAA
Cuts at: 221 1111 221
Size: 890 3222
DrdI GACnn_nn'nnGTC
Cuts at: 1818 2676 1818
Size: 858 3254
EaeI y'GGCC_r
Cuts at: 1697 1871 2262 2289 3634 1697
Size: 174 391 27 1345 2175
Fragments arranged by size:
2175 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1697 1697
Size: 4112
EarI CTCTTCn'nnn_
Cuts at: 778 2135 2345 778
Size: 1357 210 2545
Fragments arranged by size:
2545 1357 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2320 2630 2776 3922 2320
Size: 310 146 1146 2510
Fragments arranged by size:
2510 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1124 1124
Size: 4112
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 458 971 1937 2369 3116 3384 458
Size: 513 966 432 747 268 1186
Fragments arranged by size:
1186 966 747 513 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1251 1251
Size: 4112
EcoRI G'AATT_C
Cuts at: 1116 1116
Size: 4112
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 385 2131 385
Size: 1746 2366
FspI TGC'GCA
Cuts at: 1893 1893
Size: 4112
HaeII r_GCGC'y
Cuts at: 1546 1794 2816 3795 1546
Size: 248 1022 979 1863
Fragments arranged by size:
1863 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1954 1984 1986 2016 1954
Size: 30 2 30 4050
Fragments arranged by size:
4050 30 30 2
HincII GTy'rAC
Cuts at: 1151 1151
Size: 4112
KasI G'GCGC_C
Cuts at: 1542 1790 1542
Size: 248 3864
KpnI G_GTAC'C
Cuts at: 1132 1132
Size: 4112
MfeI C'AATT_G
Cuts at: 992 992
Size: 4112
MluI A'CGCG_T
Cuts at: 3545 3545
Size: 4112
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 876 921 984 2783 2967 876
Size: 45 63 1799 184 2021
Fragments arranged by size:
2021 1799 184 63 45
MscI TGG'CCA
Cuts at: 1873 1873
Size: 4112
MslI CAynn'nnrTG
Cuts at: 194 431 538 2101 2228 194
Size: 237 107 1563 127 2078
Fragments arranged by size:
2078 1563 237 127 107
MspA1I CmG'CkG
Cuts at: 1223 1537 1897 2910 3155 1223
Size: 314 360 1013 245 2180
Fragments arranged by size:
2180 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2293 2293
Size: 4112
NarI GG'CG_CC
Cuts at: 1543 1791 1543
Size: 248 3864
NgoMIV G'CCGG_C
Cuts at: 2291 2291
Size: 4112
NspI r_CATG'y
Cuts at: 193 835 891 1165 1665 2572 193
Size: 642 56 274 500 907 1733
Fragments arranged by size:
1733 907 642 500 274 56
PciI A'CATG_T
Cuts at: 2568 2568
Size: 4112
PmeI GTTT'AAAC
Cuts at: 1111 1111
Size: 4112
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3251 3283 3251
Size: 32 4080
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 642 674 642
Size: 32 4080
PstI C_TGCA'G
Cuts at: 237 636 840 1159 1844 237
Size: 399 204 319 685 2505
Fragments arranged by size:
2505 685 399 319 204
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4112
PvuII CAG'CTG
Cuts at: 1537 1897 1537
Size: 360 3752
RsrII CG'GwC_CG
Cuts at: 2307 2307
Size: 4112
SacI G_AGCT'C
Cuts at: 1126 1126
Size: 4112
SalI G'TCGA_C
Cuts at: 1149 1149
Size: 4112
SapI GCTCTTCn'nnn_
Cuts at: 2135 2345 2135
Size: 210 3902
SbfI CC_TGCA'GG
Cuts at: 1159 1159
Size: 4112
ScaI AGT'ACT
Cuts at: 678 3629 678
Size: 2951 1161
SfcI C'TryA_G
Cuts at: 33 233 632 836 1155 1840 2833 3024 33
Size: 200 399 204 319 685 993 191 1121
Fragments arranged by size:
1121 993 685 399 319 204 200 191
SfoI GGC'GCC
Cuts at: 1544 1792 1544
Size: 248 3864
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4112
SmaI CCC'GGG
Cuts at: 1134 1134
Size: 4112
SmlI C'TyrA_G
Cuts at: 487 1267 2182 2674 2936 487
Size: 780 915 492 262 1663
Fragments arranged by size:
1663 915 780 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4112
SphI G_CATG'C
Cuts at: 193 1165 1665 193
Size: 972 500 2640
Fragments arranged by size:
2640 972 500
StuI AGG'CCT
Cuts at: 365 365
Size: 4112
StyI C'CwwG_G
Cuts at: 246 1246 1600 246
Size: 1000 354 2758
Fragments arranged by size:
2758 1000 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 225 1695 1828 2287 2496 225
Size: 1470 133 459 209 1841
Fragments arranged by size:
1841 1470 459 209 133
TatI w'GTAC_w
Cuts at: 356 571 676 3627 356
Size: 215 105 2951 841
Fragments arranged by size:
2951 841 215 105
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 242 274 3629 3661 242
Size: 32 3355 32 693
Fragments arranged by size:
3355 693 32 32
Tth111I GACn'n_nGTC
Cuts at: 1909 1909
Size: 4112
XbaI T'CTAG_A
Cuts at: 1143 1143
Size: 4112
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 967 1607 967
Size: 640 3472
XmaI C'CCGG_G
Cuts at: 1132 1132
Size: 4112
XmnI GAAnn'nnTTC
Cuts at: 412 412
Size: 4112
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PciI
PmeI PpiI PsrI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI Bpu10I BseRI BsgI BsiWI BsmI BsmBI
BssHII BstEII BstZ17I Bsu36I ClaI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI NcoI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PfoI PmlI PpuMI
PshAI PsiI PspOMI PspXI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI ZraI