Restriction map for
pF1KB3618
(Circular) (Six-base) MAPSORT of: pF1KB3618.seq Check: 2792 from: 1 to: 3998
pF1KB3618 3998 bp
With 182 enzymes: SgfI *
April 24, 2009 13:02 ..
AccI GT'mk_AC
Cuts at: 1036 1036
Size: 3998
Acc65I G'GTAC_C
Cuts at: 1014 1014
Size: 3998
AclI AA'CG_TT
Cuts at: 3838 3838
Size: 3998
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 217 742 796 1823 2255 3002 3270 217
Size: 525 54 1027 432 747 268 945
Fragments arranged by size:
1027 945 747 525 432 268 54
AfeI AGC'GCT
Cuts at: 829 829
Size: 3998
AflIII A'CryG_T
Cuts at: 1350 2454 3431 1350
Size: 1104 977 1917
Fragments arranged by size:
1917 1104 977
AgeI A'CCGG_T
Cuts at: 2386 2386
Size: 3998
AlwNI CAG_nnn'CTG
Cuts at: 1303 2870 1303
Size: 1567 2431
ApaI G_GGCC'C
Cuts at: 989 989
Size: 3998
ApaLI G'TGCA_C
Cuts at: 2768 2768
Size: 3998
ApoI r'AATT_y
Cuts at: 1002 3351 3607 1002
Size: 2349 256 1393
Fragments arranged by size:
2349 1393 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3998
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3998
AvaI C'yCGr_G
Cuts at: 1018 1018
Size: 3998
BamHI G'GATC_C
Cuts at: 1023 1023
Size: 3998
BanI G'GyrC_C
Cuts at: 1014 1428 1676 1711 1014
Size: 414 248 35 3301
Fragments arranged by size:
3301 414 248 35
BanII G_rGCy'C
Cuts at: 547 989 1012 2042 547
Size: 442 23 1030 2503
Fragments arranged by size:
2503 1030 442 23
BbeI G_GCGC'C
Cuts at: 1432 1680 1432
Size: 248 3750
BbsI GAAGACnn'nnnn_
Cuts at: 224 750 224
Size: 526 3472
BbvCI CC'TCA_GC
Cuts at: 484 484
Size: 3998
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 519 553 519
Size: 34 3964
BciVI GTATCCnnnnn_n'
Cuts at: 1896 2657 3998 1896
Size: 761 1341 1896
Fragments arranged by size:
1896 1341 761
BclI T'GATC_A
Cuts at: 737 1518 737
Size: 781 3217
BglI GCCn_nnn'nGGC
Cuts at: 874 874
Size: 3998
BglII A'GATC_T
Cuts at: 648 1513 3550 648
Size: 865 2037 1096
Fragments arranged by size:
2037 1096 865
BlpI GC'TnA_GC
Cuts at: 1110 1110
Size: 3998
Bme1580I G_kGCm'C
Cuts at: 989 1623 1716 2772 989
Size: 634 93 1056 2215
Fragments arranged by size:
2215 1056 634 93
BmrI ACTGGGnnnn_n'
Cuts at: 282 616 801 1210 1291 1626 3770 282
Size: 334 185 409 81 335 2144 510
Fragments arranged by size:
2144 510 409 335 334 185 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2365 2365
Size: 3998
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 205 391 907 205
Size: 186 516 3296
Fragments arranged by size:
3296 516 186
Bpu10I CC'TnA_GC
Cuts at: 172 484 172
Size: 312 3686
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 948 1174 2053 2545 2843 948
Size: 226 879 492 298 2103
Fragments arranged by size:
2103 879 492 298 226
BsaI GGTCTCn'nnnn_
Cuts at: 3702 3702
Size: 3998
BsaAI yAC'GTr
Cuts at: 1351 1351
Size: 3998
BsaBI GATnn'nnATC
Cuts at: 6 868 1512 3549 6
Size: 862 644 2037 455
Fragments arranged by size:
2037 862 644 455
BsaHI Gr'CG_yC
Cuts at: 839 1429 1677 2379 3875 839
Size: 590 248 702 1496 962
Fragments arranged by size:
1496 962 702 590 248
BsaWI w'CCGG_w
Cuts at: 1187 1708 2386 2660 2807 3218 1187
Size: 521 678 274 147 411 1967
Fragments arranged by size:
1967 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 103 133 367 397 103
Size: 30 234 30 3704
Fragments arranged by size:
3704 234 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 388 388
Size: 3998
BseYI C'CCAG_C
Cuts at: 403 642 939 1423 2181 2758 3254 403
Size: 239 297 484 758 577 496 1147
Fragments arranged by size:
1147 758 577 496 484 297 239
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 485 485
Size: 3998
BsiEI CG_ry'CG
Cuts at: 104 127 1540 1586 2794 104
Size: 23 1413 46 1208 1308
Fragments arranged by size:
1413 1308 1208 46 23
BsiHKAI G_wGCw'C
Cuts at: 173 1012 1790 1980 2772 173
Size: 839 778 190 792 1399
Fragments arranged by size:
1399 839 792 778 190
BsmI GAATG_Cn'
Cuts at: 295 395 295
Size: 100 3898
Bsp1286I G_dGCh'C
Cuts at: 173 547 989 1012 1623 1716 1790 1980 2042
Size: 374 442 23 611 93 74 190 62
Cuts at: 2042 2772 173
Size: 730 1399
Fragments arranged by size:
1399 730 611 442 374 190 93 74
62 23
BspEI T'CCGG_A
Cuts at: 3218 3218
Size: 3998
BspHI T'CATG_A
Cuts at: 3174 3174
Size: 3998
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1048 1262 1564 1945 1048
Size: 214 302 381 3101
Fragments arranged by size:
3101 381 302 214
BsrBI CCG'CTC
Cuts at: 50 129 2290 2344 3828 3993 50
Size: 79 2161 54 1484 165 55
Fragments arranged by size:
2161 1484 165 79 55 54
BsrDI GCAATG_nn'
Cuts at: 1910 1910
Size: 3998
BsrFI r'CCGG_y
Cuts at: 1996 2177 2386 1996
Size: 181 209 3608
Fragments arranged by size:
3608 209 181
BssSI C'ACGA_G
Cuts at: 2269 2627 2269
Size: 358 3640
BstAPI GCAn_nnn'nTGC
Cuts at: 652 1375 652
Size: 723 3275
BstBI TT'CG_AA
Cuts at: 2359 2359
Size: 3998
BstYI r'GATC_y
Cuts at: 1 648 1023 1490 1513 1848 2094 3106 3192
Size: 647 375 467 23 335 246 1012 86
Cuts at: 3192 3204 3550 1
Size: 12 346 449
Fragments arranged by size:
1012 647 467 449 375 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3998
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1948 2129 2292 1948
Size: 181 163 3654
Fragments arranged by size:
3654 181 163
BtsI GCAGTG_nn'
Cuts at: 1241 1241
Size: 3998
DraI TTT'AAA
Cuts at: 997 997
Size: 3998
DrdI GACnn_nn'nnGTC
Cuts at: 1704 2562 1704
Size: 858 3140
EaeI y'GGCC_r
Cuts at: 672 1583 1757 2148 2175 3520 672
Size: 911 174 391 27 1345 1150
Fragments arranged by size:
1345 1150 911 391 174 27
EagI C'GGCC_G
Cuts at: 1583 1583
Size: 3998
EarI CTCTTCn'nnn_
Cuts at: 2021 2231 2021
Size: 210 3788
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 898 2206 2516 2662 3808 898
Size: 1308 310 146 1146 1088
Fragments arranged by size:
1308 1146 1088 310 146
EcoICRI GAG'CTC
Cuts at: 1010 1010
Size: 3998
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 205 217 391 742 796 907 1823 2255 3002
Size: 12 174 351 54 111 916 432 747
Cuts at: 3002 3270 205
Size: 268 933
Fragments arranged by size:
933 916 747 432 351 268 174 111
54 12
EcoNI CCTnn'n_nnAGG
Cuts at: 889 910 889
Size: 21 3977
EcoO109I rG'GnC_Cy
Cuts at: 176 304 618 986 1137 176
Size: 128 314 368 151 3037
Fragments arranged by size:
3037 368 314 151 128
EcoRI G'AATT_C
Cuts at: 1002 1002
Size: 3998
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2017 2017
Size: 3998
FspI TGC'GCA
Cuts at: 1779 1779
Size: 3998
HaeII r_GCGC'y
Cuts at: 831 1432 1680 2702 3681 831
Size: 601 248 1022 979 1148
Fragments arranged by size:
1148 1022 979 601 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 103 135 367 399 1840 1870 1872 1902 103
Size: 32 232 32 1441 30 2 30 2199
Fragments arranged by size:
2199 1441 232 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1037 1037
Size: 3998
KasI G'GCGC_C
Cuts at: 1428 1676 1428
Size: 248 3750
KpnI G_GTAC'C
Cuts at: 1018 1018
Size: 3998
MluI A'CGCG_T
Cuts at: 3431 3431
Size: 3998
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 335 2669 2853 335
Size: 2334 184 1480
Fragments arranged by size:
2334 1480 184
MscI TGG'CCA
Cuts at: 674 1759 674
Size: 1085 2913
MslI CAynn'nnrTG
Cuts at: 1987 2114 1987
Size: 127 3871
MspA1I CmG'CkG
Cuts at: 195 564 1109 1423 1783 2796 3041 195
Size: 369 545 314 360 1013 245 1152
Fragments arranged by size:
1152 1013 545 369 360 314 245
NaeI GCC'GGC
Cuts at: 2179 2179
Size: 3998
NarI GG'CG_CC
Cuts at: 1429 1677 1429
Size: 248 3750
NcoI C'CATG_G
Cuts at: 107 107
Size: 3998
NgoMIV G'CCGG_C
Cuts at: 2177 2177
Size: 3998
NspI r_CATG'y
Cuts at: 1051 1551 2458 1051
Size: 500 907 2591
Fragments arranged by size:
2591 907 500
PciI A'CATG_T
Cuts at: 2454 2454
Size: 3998
PflMI CCAn_nnn'nTGG
Cuts at: 918 918
Size: 3998
PfoI T'CCnGG_A
Cuts at: 884 884
Size: 3998
PmeI GTTT'AAAC
Cuts at: 997 997
Size: 3998
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3137 3169 3137
Size: 32 3966
PpuMI rG'GwC_Cy
Cuts at: 176 618 176
Size: 442 3556
PspOMI G'GGCC_C
Cuts at: 985 985
Size: 3998
PstI C_TGCA'G
Cuts at: 449 1045 1730 449
Size: 596 685 2717
Fragments arranged by size:
2717 685 596
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3998
PvuII CAG'CTG
Cuts at: 564 1423 1783 564
Size: 859 360 2779
Fragments arranged by size:
2779 859 360
RsrII CG'GwC_CG
Cuts at: 2193 2193
Size: 3998
SacI G_AGCT'C
Cuts at: 1012 1012
Size: 3998
SalI G'TCGA_C
Cuts at: 1035 1035
Size: 3998
SapI GCTCTTCn'nnn_
Cuts at: 2021 2231 2021
Size: 210 3788
SbfI CC_TGCA'GG
Cuts at: 1045 1045
Size: 3998
ScaI AGT'ACT
Cuts at: 790 3515 790
Size: 2725 1273
SexAI A'CCwGG_T
Cuts at: 555 555
Size: 3998
SfcI C'TryA_G
Cuts at: 33 445 1041 1726 2719 2910 33
Size: 412 596 685 993 191 1121
Fragments arranged by size:
1121 993 685 596 412 191
SfoI GGC'GCC
Cuts at: 1430 1678 1430
Size: 248 3750
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3998
SmaI CCC'GGG
Cuts at: 1020 1020
Size: 3998
SmlI C'TyrA_G
Cuts at: 927 1153 2068 2560 2822 927
Size: 226 915 492 262 2103
Fragments arranged by size:
2103 915 492 262 226
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3998
SphI G_CATG'C
Cuts at: 1051 1551 1051
Size: 500 3498
StyI C'CwwG_G
Cuts at: 107 1132 1486 107
Size: 1025 354 2619
Fragments arranged by size:
2619 1025 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 462 1581 1714 2173 2382 462
Size: 1119 133 459 209 2078
Fragments arranged by size:
2078 1119 459 209 133
TatI w'GTAC_w
Cuts at: 788 3513 788
Size: 2725 1273
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3515 3547 3515
Size: 32 3966
Tth111I GACn'n_nGTC
Cuts at: 557 1795 557
Size: 1238 2760
XbaI T'CTAG_A
Cuts at: 1029 1029
Size: 3998
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1493 1493
Size: 3998
XmaI C'CCGG_G
Cuts at: 1018 1018
Size: 3998
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI
PfoI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BfrBI BmgBI BmtI BsiWI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PmlI PshAI PsiI
PspXI PsrI SacII SanDI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI ZraI