Restriction map for
pF1KB3614
(Circular) (Six-base) MAPSORT of: pF1KB3614.seq Check: 7598 from: 1 to: 4367
pF1KB3614 4367 bp
With 182 enzymes: SgfI *
April 24, 2009 13:02 ..
AccI GT'mk_AC
Cuts at: 1405 1405
Size: 4367
Acc65I G'GTAC_C
Cuts at: 1383 1383
Size: 4367
AclI AA'CG_TT
Cuts at: 4207 4207
Size: 4367
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2192 2624 3371 3639 2192
Size: 432 747 268 2920
Fragments arranged by size:
2920 747 432 268
AflIII A'CryG_T
Cuts at: 1286 1719 2823 3800 1286
Size: 433 1104 977 1853
Fragments arranged by size:
1853 1104 977 433
AgeI A'CCGG_T
Cuts at: 2755 2755
Size: 4367
AlwNI CAG_nnn'CTG
Cuts at: 406 1672 3239 406
Size: 1266 1567 1534
Fragments arranged by size:
1567 1534 1266
ApaLI G'TGCA_C
Cuts at: 3137 3137
Size: 4367
ApoI r'AATT_y
Cuts at: 710 873 1182 1371 3720 3976 710
Size: 163 309 189 2349 256 1101
Fragments arranged by size:
2349 1101 309 256 189 163
AseI AT'TA_AT
Cuts at: 20 20
Size: 4367
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4367
AvaI C'yCGr_G
Cuts at: 1387 1387
Size: 4367
AvrII C'CTAG_G
Cuts at: 781 781
Size: 4367
BamHI G'GATC_C
Cuts at: 1392 1392
Size: 4367
BanI G'GyrC_C
Cuts at: 1383 1797 2045 2080 1383
Size: 414 248 35 3670
Fragments arranged by size:
3670 414 248 35
BanII G_rGCy'C
Cuts at: 1200 1381 2411 1200
Size: 181 1030 3156
Fragments arranged by size:
3156 1030 181
BbeI G_GCGC'C
Cuts at: 1801 2049 1801
Size: 248 4119
BciVI GTATCCnnnnn_n'
Cuts at: 2265 3026 4367 2265
Size: 761 1341 2265
Fragments arranged by size:
2265 1341 761
BclI T'GATC_A
Cuts at: 1887 1887
Size: 4367
BglII A'GATC_T
Cuts at: 1882 3919 1882
Size: 2037 2330
BlpI GC'TnA_GC
Cuts at: 1479 1479
Size: 4367
Bme1580I G_kGCm'C
Cuts at: 1992 2085 3141 1992
Size: 93 1056 3218
Fragments arranged by size:
3218 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1579 1660 1995 4139 1579
Size: 81 335 2144 1807
Fragments arranged by size:
2144 1807 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2734 2734
Size: 4367
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 848 1214 848
Size: 366 4001
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 197 1543 2422 2914 3212 197
Size: 1346 879 492 298 1352
Fragments arranged by size:
1352 1346 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4071 4071
Size: 4367
BsaAI yAC'GTr
Cuts at: 1720 1720
Size: 4367
BsaBI GATnn'nnATC
Cuts at: 6 1881 3918 6
Size: 1875 2037 455
Fragments arranged by size:
2037 1875 455
BsaHI Gr'CG_yC
Cuts at: 609 1798 2046 2748 4244 609
Size: 1189 248 702 1496 732
Fragments arranged by size:
1496 1189 732 702 248
BsaWI w'CCGG_w
Cuts at: 139 818 1556 2077 2755 3029 3176 3587 139
Size: 679 738 521 678 274 147 411 919
Fragments arranged by size:
919 738 679 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1142 1172 1142
Size: 30 4337
BseYI C'CCAG_C
Cuts at: 1792 2550 3127 3623 1792
Size: 758 577 496 2536
Fragments arranged by size:
2536 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1909 1955 3163 104
Size: 1805 46 1208 1308
Fragments arranged by size:
1805 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1381 2159 2349 3141 1381
Size: 778 190 792 2607
Fragments arranged by size:
2607 792 778 190
BsmI GAATG_Cn'
Cuts at: 755 755
Size: 4367
Bsp1286I G_dGCh'C
Cuts at: 1200 1381 1992 2085 2159 2349 2411 3141 1200
Size: 181 611 93 74 190 62 730 2426
Fragments arranged by size:
2426 730 611 190 181 93 74 62
BspEI T'CCGG_A
Cuts at: 3587 3587
Size: 4367
BspHI T'CATG_A
Cuts at: 773 3543 773
Size: 2770 1597
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1417 1631 1933 2314 1417
Size: 214 302 381 3470
Fragments arranged by size:
3470 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2659 2713 4197 4362 50
Size: 2609 54 1484 165 55
Fragments arranged by size:
2609 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2279 2279
Size: 4367
BsrFI r'CCGG_y
Cuts at: 2365 2546 2755 2365
Size: 181 209 3977
Fragments arranged by size:
3977 209 181
BssSI C'ACGA_G
Cuts at: 2638 2996 2638
Size: 358 4009
BstAPI GCAn_nnn'nTGC
Cuts at: 1744 1744
Size: 4367
BstBI TT'CG_AA
Cuts at: 2728 2728
Size: 4367
BstYI r'GATC_y
Cuts at: 1 691 1241 1392 1859 1882 2217 2463 3475
Size: 690 550 151 467 23 335 246 1012
Cuts at: 3475 3561 3573 3919 1
Size: 86 12 346 449
Fragments arranged by size:
1012 690 550 467 449 346 335 246
151 86 23 12
BtgI C'CryG_G
Cuts at: 604 604
Size: 4367
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2317 2498 2661 2317
Size: 181 163 4023
Fragments arranged by size:
4023 181 163
BtsI GCAGTG_nn'
Cuts at: 1610 1610
Size: 4367
DraI TTT'AAA
Cuts at: 1314 1366 1314
Size: 52 4315
DrdI GACnn_nn'nnGTC
Cuts at: 2073 2931 2073
Size: 858 3509
EaeI y'GGCC_r
Cuts at: 1952 2126 2517 2544 3889 1952
Size: 174 391 27 1345 2430
Fragments arranged by size:
2430 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1952 1952
Size: 4367
EarI CTCTTCn'nnn_
Cuts at: 163 304 1205 2390 2600 163
Size: 141 901 1185 210 1930
Fragments arranged by size:
1930 1185 901 210 141
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2575 2885 3031 4177 2575
Size: 310 146 1146 2765
Fragments arranged by size:
2765 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1379 1379
Size: 4367
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 848 1214 2192 2624 3371 3639 848
Size: 366 978 432 747 268 1576
Fragments arranged by size:
1576 978 747 432 366 268
EcoO109I rG'GnC_Cy
Cuts at: 1506 1506
Size: 4367
EcoRI G'AATT_C
Cuts at: 1371 1371
Size: 4367
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 318 2386 318
Size: 2068 2299
FspI TGC'GCA
Cuts at: 2148 2148
Size: 4367
HaeII r_GCGC'y
Cuts at: 1801 2049 3071 4050 1801
Size: 248 1022 979 2118
Fragments arranged by size:
2118 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 102 134 683 715 2209 2239 2241 2271 102
Size: 32 549 32 1494 30 2 30 2198
Fragments arranged by size:
2198 1494 549 32 32 30 30 2
HincII GTy'rAC
Cuts at: 540 1406 540
Size: 866 3501
KasI G'GCGC_C
Cuts at: 1797 2045 1797
Size: 248 4119
KpnI G_GTAC'C
Cuts at: 1387 1387
Size: 4367
MfeI C'AATT_G
Cuts at: 989 989
Size: 4367
MluI A'CGCG_T
Cuts at: 3800 3800
Size: 4367
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 620 903 3038 3222 620
Size: 283 2135 184 1765
Fragments arranged by size:
2135 1765 283 184
MscI TGG'CCA
Cuts at: 2128 2128
Size: 4367
MslI CAynn'nnrTG
Cuts at: 2356 2483 2356
Size: 127 4240
MspA1I CmG'CkG
Cuts at: 591 1478 1792 2152 3165 3410 591
Size: 887 314 360 1013 245 1548
Fragments arranged by size:
1548 1013 887 360 314 245
NaeI GCC'GGC
Cuts at: 2548 2548
Size: 4367
NarI GG'CG_CC
Cuts at: 1798 2046 1798
Size: 248 4119
NcoI C'CATG_G
Cuts at: 604 604
Size: 4367
NgoMIV G'CCGG_C
Cuts at: 2546 2546
Size: 4367
NspI r_CATG'y
Cuts at: 1050 1290 1420 1920 2827 1050
Size: 240 130 500 907 2590
Fragments arranged by size:
2590 907 500 240 130
PciI A'CATG_T
Cuts at: 1286 2823 1286
Size: 1537 2830
PmeI GTTT'AAAC
Cuts at: 1366 1366
Size: 4367
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3506 3538 3506
Size: 32 4335
PstI C_TGCA'G
Cuts at: 1414 2099 1414
Size: 685 3682
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4367
PvuII CAG'CTG
Cuts at: 591 1792 2152 591
Size: 1201 360 2806
Fragments arranged by size:
2806 1201 360
RsrII CG'GwC_CG
Cuts at: 2562 2562
Size: 4367
SacI G_AGCT'C
Cuts at: 1381 1381
Size: 4367
SalI G'TCGA_C
Cuts at: 1404 1404
Size: 4367
SapI GCTCTTCn'nnn_
Cuts at: 163 304 2390 2600 163
Size: 141 2086 210 1930
Fragments arranged by size:
2086 1930 210 141
SbfI CC_TGCA'GG
Cuts at: 1414 1414
Size: 4367
ScaI AGT'ACT
Cuts at: 3884 3884
Size: 4367
SfcI C'TryA_G
Cuts at: 33 695 1410 2095 3088 3279 33
Size: 662 715 685 993 191 1121
Fragments arranged by size:
1121 993 715 685 662 191
SfoI GGC'GCC
Cuts at: 1799 2047 1799
Size: 248 4119
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4367
SmaI CCC'GGG
Cuts at: 1389 1389
Size: 4367
SmlI C'TyrA_G
Cuts at: 212 1522 2437 2929 3191 212
Size: 1310 915 492 262 1388
Fragments arranged by size:
1388 1310 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4367
SphI G_CATG'C
Cuts at: 1420 1920 1420
Size: 500 3867
StyI C'CwwG_G
Cuts at: 604 781 1115 1501 1855 604
Size: 177 334 386 354 3116
Fragments arranged by size:
3116 386 354 334 177
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 337 1095 1950 2083 2542 2751 337
Size: 758 855 133 459 209 1953
Fragments arranged by size:
1953 855 758 459 209 133
TatI w'GTAC_w
Cuts at: 626 3882 626
Size: 3256 1111
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1019 1051 1142 1174 3884 3916 1019
Size: 32 91 32 2710 32 1470
Fragments arranged by size:
2710 1470 91 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2164 2164
Size: 4367
XbaI T'CTAG_A
Cuts at: 153 1398 153
Size: 1245 3122
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1862 1862
Size: 4367
XmaI C'CCGG_G
Cuts at: 1387 1387
Size: 4367
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PmeI
PpiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI Bpu10I BseRI BsgI BsiWI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PfoI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI