Restriction map for
pF1KB3608
(Circular) (Six-base) MAPSORT of: pF1KB3608.seq Check: 3638 from: 1 to: 3743
pF1KB3608 3743 bp
With 182 enzymes: SgfI *
March 2, 2010 14:25 ..
AccI GT'mk_AC
Cuts at: 781 781
Size: 3743
Acc65I G'GTAC_C
Cuts at: 759 759
Size: 3743
AclI AA'CG_TT
Cuts at: 3583 3583
Size: 3743
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 739 1568 2000 2747 3015 739
Size: 829 432 747 268 1467
Fragments arranged by size:
1467 829 747 432 268
AfeI AGC'GCT
Cuts at: 400 400
Size: 3743
AflIII A'CryG_T
Cuts at: 1095 2199 3176 1095
Size: 1104 977 1662
Fragments arranged by size:
1662 1104 977
AgeI A'CCGG_T
Cuts at: 2131 2131
Size: 3743
AleI CACnn'nnGTG
Cuts at: 319 448 319
Size: 129 3614
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 249 281 249
Size: 32 3711
AlwNI CAG_nnn'CTG
Cuts at: 1048 2615 1048
Size: 1567 2176
ApaLI G'TGCA_C
Cuts at: 2513 2513
Size: 3743
ApoI r'AATT_y
Cuts at: 688 747 3096 3352 688
Size: 59 2349 256 1079
Fragments arranged by size:
2349 1079 256 59
AseI AT'TA_AT
Cuts at: 20 20
Size: 3743
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3743
AvaI C'yCGr_G
Cuts at: 591 632 763 591
Size: 41 131 3571
Fragments arranged by size:
3571 131 41
BamHI G'GATC_C
Cuts at: 768 768
Size: 3743
BanI G'GyrC_C
Cuts at: 338 759 1173 1421 1456 338
Size: 421 414 248 35 2625
Fragments arranged by size:
2625 421 414 248 35
BanII G_rGCy'C
Cuts at: 757 1787 757
Size: 1030 2713
BbeI G_GCGC'C
Cuts at: 342 1177 1425 342
Size: 835 248 2660
Fragments arranged by size:
2660 835 248
BbsI GAAGACnn'nnnn_
Cuts at: 539 539
Size: 3743
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3709
BciVI GTATCCnnnnn_n'
Cuts at: 1641 2402 3743 1641
Size: 761 1341 1641
Fragments arranged by size:
1641 1341 761
BclI T'GATC_A
Cuts at: 1263 1263
Size: 3743
BglII A'GATC_T
Cuts at: 329 1258 3295 329
Size: 929 2037 777
Fragments arranged by size:
2037 929 777
BlpI GC'TnA_GC
Cuts at: 281 855 281
Size: 574 3169
Bme1580I G_kGCm'C
Cuts at: 1368 1461 2517 1368
Size: 93 1056 2594
Fragments arranged by size:
2594 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 955 1036 1371 3515 955
Size: 81 335 2144 1183
Fragments arranged by size:
2144 1183 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 260 2110 260
Size: 1850 1893
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 242 253 242
Size: 11 3732
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 919 1798 2290 2588 919
Size: 879 492 298 2074
Fragments arranged by size:
2074 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 158 718 3447 158
Size: 560 2729 454
Fragments arranged by size:
2729 560 454
BsaAI yAC'GTr
Cuts at: 1096 1096
Size: 3743
BsaBI GATnn'nnATC
Cuts at: 6 1257 3294 6
Size: 1251 2037 455
Fragments arranged by size:
2037 1251 455
BsaHI Gr'CG_yC
Cuts at: 339 1174 1422 2124 3620 339
Size: 835 248 702 1496 462
Fragments arranged by size:
1496 835 702 462 248
BsaWI w'CCGG_w
Cuts at: 683 932 1453 2131 2405 2552 2963 683
Size: 249 521 678 274 147 411 1463
Fragments arranged by size:
1463 678 521 411 274 249 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 249 279 249
Size: 30 3713
BseYI C'CCAG_C
Cuts at: 1168 1926 2503 2999 1168
Size: 758 577 496 1912
Fragments arranged by size:
1912 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 142 415 451 472 554 142
Size: 273 36 21 82 3331
Fragments arranged by size:
3331 273 82 36 21
BsiEI CG_ry'CG
Cuts at: 104 1285 1331 2539 104
Size: 1181 46 1208 1308
Fragments arranged by size:
1308 1208 1181 46
BsiHKAI G_wGCw'C
Cuts at: 757 1535 1725 2517 757
Size: 778 190 792 1983
Fragments arranged by size:
1983 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 757 1368 1461 1535 1725 1787 2517 757
Size: 611 93 74 190 62 730 1983
Fragments arranged by size:
1983 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2963 2963
Size: 3743
BspHI T'CATG_A
Cuts at: 2919 2919
Size: 3743
BspMI ACCTGCnnnn'nnnn_
Cuts at: 793 1007 1309 1690 793
Size: 214 302 381 2846
Fragments arranged by size:
2846 381 302 214
BsrBI CCG'CTC
Cuts at: 50 131 212 2035 2089 3573 3738 50
Size: 81 81 1823 54 1484 165 55
Fragments arranged by size:
1823 1484 165 81 81 55 54
BsrDI GCAATG_nn'
Cuts at: 275 1655 275
Size: 1380 2363
BsrFI r'CCGG_y
Cuts at: 285 662 1741 1922 2131 285
Size: 377 1079 181 209 1897
Fragments arranged by size:
1897 1079 377 209 181
BssSI C'ACGA_G
Cuts at: 245 2014 2372 245
Size: 1769 358 1616
Fragments arranged by size:
1769 1616 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1120 1120
Size: 3743
BstBI TT'CG_AA
Cuts at: 2104 2104
Size: 3743
BstEII G'GTnAC_C
Cuts at: 636 636
Size: 3743
BstXI CCAn_nnnn'nTGG
Cuts at: 369 369
Size: 3743
BstYI r'GATC_y
Cuts at: 1 329 768 1235 1258 1593 1839 2851 2937
Size: 328 439 467 23 335 246 1012 86
Cuts at: 2937 2949 3295 1
Size: 12 346 449
Fragments arranged by size:
1012 467 449 439 346 335 328 246
86 23 12
BtgI C'CryG_G
Cuts at: 518 518
Size: 3743
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1693 1874 2037 1693
Size: 181 163 3399
Fragments arranged by size:
3399 181 163
BtsI GCAGTG_nn'
Cuts at: 439 986 439
Size: 547 3196
DraI TTT'AAA
Cuts at: 742 742
Size: 3743
DrdI GACnn_nn'nnGTC
Cuts at: 1449 2307 1449
Size: 858 2885
EaeI y'GGCC_r
Cuts at: 287 376 1328 1502 1893 1920 3265 287
Size: 89 952 174 391 27 1345 765
Fragments arranged by size:
1345 952 765 391 174 89 27
EagI C'GGCC_G
Cuts at: 1328 1328
Size: 3743
EarI CTCTTCn'nnn_
Cuts at: 1766 1976 1766
Size: 210 3533
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 649 1951 2261 2407 3553 649
Size: 1302 310 146 1146 839
Fragments arranged by size:
1302 1146 839 310 146
EcoICRI GAG'CTC
Cuts at: 755 755
Size: 3743
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 242 253 739 1568 2000 2747 3015 242
Size: 11 486 829 432 747 268 970
Fragments arranged by size:
970 829 747 486 432 268 11
EcoO109I rG'GnC_Cy
Cuts at: 882 882
Size: 3743
EcoRI G'AATT_C
Cuts at: 747 747
Size: 3743
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 522 1762 522
Size: 1240 2503
FspI TGC'GCA
Cuts at: 1524 1524
Size: 3743
HaeII r_GCGC'y
Cuts at: 342 402 1177 1425 2447 3426 342
Size: 60 775 248 1022 979 659
Fragments arranged by size:
1022 979 775 659 248 60
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1585 1615 1617 1647 1585
Size: 30 2 30 3681
Fragments arranged by size:
3681 30 30 2
HincII GTy'rAC
Cuts at: 782 782
Size: 3743
KasI G'GCGC_C
Cuts at: 338 1173 1421 338
Size: 835 248 2660
Fragments arranged by size:
2660 835 248
KpnI G_GTAC'C
Cuts at: 763 763
Size: 3743
MluI A'CGCG_T
Cuts at: 3176 3176
Size: 3743
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 171 173 2414 2598 171
Size: 2 2241 184 1316
Fragments arranged by size:
2241 1316 184 2
MscI TGG'CCA
Cuts at: 1504 1504
Size: 3743
MslI CAynn'nnrTG
Cuts at: 319 448 549 701 1732 1859 319
Size: 129 101 152 1031 127 2203
Fragments arranged by size:
2203 1031 152 129 127 101
MspA1I CmG'CkG
Cuts at: 456 568 854 1168 1528 2541 2786 456
Size: 112 286 314 360 1013 245 1413
Fragments arranged by size:
1413 1013 360 314 286 245 112
NaeI GCC'GGC
Cuts at: 287 664 1924 287
Size: 377 1260 2106
Fragments arranged by size:
2106 1260 377
NarI GG'CG_CC
Cuts at: 339 1174 1422 339
Size: 835 248 2660
Fragments arranged by size:
2660 835 248
NgoMIV G'CCGG_C
Cuts at: 285 662 1922 285
Size: 377 1260 2106
Fragments arranged by size:
2106 1260 377
NspI r_CATG'y
Cuts at: 548 796 1296 2203 548
Size: 248 500 907 2088
Fragments arranged by size:
2088 907 500 248
PciI A'CATG_T
Cuts at: 2199 2199
Size: 3743
PmeI GTTT'AAAC
Cuts at: 742 742
Size: 3743
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 249 281 2882 2914 249
Size: 32 2601 32 1078
Fragments arranged by size:
2601 1078 32 32
PstI C_TGCA'G
Cuts at: 790 1475 790
Size: 685 3058
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3743
PvuII CAG'CTG
Cuts at: 456 568 1168 1528 456
Size: 112 600 360 2671
Fragments arranged by size:
2671 600 360 112
RsrII CG'GwC_CG
Cuts at: 1938 1938
Size: 3743
SacI G_AGCT'C
Cuts at: 757 757
Size: 3743
SalI G'TCGA_C
Cuts at: 780 780
Size: 3743
SapI GCTCTTCn'nnn_
Cuts at: 1766 1976 1766
Size: 210 3533
SbfI CC_TGCA'GG
Cuts at: 790 790
Size: 3743
ScaI AGT'ACT
Cuts at: 3260 3260
Size: 3743
SfcI C'TryA_G
Cuts at: 33 786 1471 2464 2655 33
Size: 753 685 993 191 1121
Fragments arranged by size:
1121 993 753 685 191
SfoI GGC'GCC
Cuts at: 340 1175 1423 340
Size: 835 248 2660
Fragments arranged by size:
2660 835 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3743
SmaI CCC'GGG
Cuts at: 593 765 593
Size: 172 3571
SmlI C'TyrA_G
Cuts at: 898 1813 2305 2567 898
Size: 915 492 262 2074
Fragments arranged by size:
2074 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3743
SphI G_CATG'C
Cuts at: 548 796 1296 548
Size: 248 500 2995
Fragments arranged by size:
2995 500 248
StyI C'CwwG_G
Cuts at: 671 729 877 1231 671
Size: 58 148 354 3183
Fragments arranged by size:
3183 354 148 58
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 460 1326 1459 1918 2127 460
Size: 866 133 459 209 2076
Fragments arranged by size:
2076 866 459 209 133
TatI w'GTAC_w
Cuts at: 3258 3258
Size: 3743
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3260 3292 3260
Size: 32 3711
Tth111I GACn'n_nGTC
Cuts at: 1540 1540
Size: 3743
XbaI T'CTAG_A
Cuts at: 774 774
Size: 3743
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 525 1238 525
Size: 713 3030
XmaI C'CCGG_G
Cuts at: 591 763 591
Size: 172 3571
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PciI PmeI PpiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AlfI ApaI AscI AvrII
BaeI BbvCI BfrBI BglI BmgBI BmtI Bpu10I BseRI
BsiWI BsmI BsmBI BsrGI BssHII BstZ17I Bsu36I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NcoI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI