Restriction map for
pF1KB3604
(Circular) (Six-base) MAPSORT of: pF1KB3604.seq Check: 2783 from: 1 to: 4613
pF1KB3604 4613 bp
With 182 enzymes: SgfI *
April 24, 2009 13:02 ..
AccI GT'mk_AC
Cuts at: 212 1651 212
Size: 1439 3174
Acc65I G'GTAC_C
Cuts at: 641 1382 1629 641
Size: 741 247 3625
Fragments arranged by size:
3625 741 247
AclI AA'CG_TT
Cuts at: 4453 4453
Size: 4613
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1155 1530 2438 2870 3617 3885 1155
Size: 375 908 432 747 268 1883
Fragments arranged by size:
1883 908 747 432 375 268
AflIII A'CryG_T
Cuts at: 1965 3069 4046 1965
Size: 1104 977 2532
Fragments arranged by size:
2532 1104 977
AgeI A'CCGG_T
Cuts at: 3001 3001
Size: 4613
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 400 400
Size: 4613
AlwNI CAG_nnn'CTG
Cuts at: 135 316 1918 3485 135
Size: 181 1602 1567 1263
Fragments arranged by size:
1602 1567 1263 181
ApaI G_GGCC'C
Cuts at: 1128 1128
Size: 4613
ApaLI G'TGCA_C
Cuts at: 3383 3383
Size: 4613
ApoI r'AATT_y
Cuts at: 220 1617 3966 4222 220
Size: 1397 2349 256 611
Fragments arranged by size:
2349 1397 611 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4613
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4613
AvaI C'yCGr_G
Cuts at: 1633 1633
Size: 4613
BamHI G'GATC_C
Cuts at: 178 1638 178
Size: 1460 3153
BanI G'GyrC_C
Cuts at: 171 641 1382 1417 1629 2043 2291 2326 171
Size: 470 741 35 212 414 248 35 2458
Fragments arranged by size:
2458 741 470 414 248 212 35 35
BanII G_rGCy'C
Cuts at: 989 1128 1627 2657 989
Size: 139 499 1030 2945
Fragments arranged by size:
2945 1030 499 139
BbeI G_GCGC'C
Cuts at: 2047 2295 2047
Size: 248 4365
BbsI GAAGACnn'nnnn_
Cuts at: 253 967 1536 253
Size: 714 569 3330
Fragments arranged by size:
3330 714 569
BbvCI CC'TCA_GC
Cuts at: 1286 1286
Size: 4613
BciVI GTATCCnnnnn_n'
Cuts at: 2511 3272 4613 2511
Size: 761 1341 2511
Fragments arranged by size:
2511 1341 761
BclI T'GATC_A
Cuts at: 2133 2133
Size: 4613
BglI GCCn_nnn'nGGC
Cuts at: 1345 1345
Size: 4613
BglII A'GATC_T
Cuts at: 268 404 2128 4165 268
Size: 136 1724 2037 716
Fragments arranged by size:
2037 1724 716 136
BlpI GC'TnA_GC
Cuts at: 780 1725 780
Size: 945 3668
Bme1580I G_kGCm'C
Cuts at: 1128 2238 2331 3387 1128
Size: 1110 93 1056 2354
Fragments arranged by size:
2354 1110 1056 93
BmgBI CAC'GTC
Cuts at: 695 1161 695
Size: 466 4147
BmrI ACTGGGnnnn_n'
Cuts at: 1146 1199 1383 1825 1906 2241 4385 1146
Size: 53 184 442 81 335 2144 1374
Fragments arranged by size:
2144 1374 442 335 184 81 53
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2980 2980
Size: 4613
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 709 1357 709
Size: 648 3965
Bpu10I CC'TnA_GC
Cuts at: 1286 1286
Size: 4613
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1789 2668 3160 3458 1789
Size: 879 492 298 2944
Fragments arranged by size:
2944 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4317 4317
Size: 4613
BsaAI yAC'GTr
Cuts at: 1966 1966
Size: 4613
BsaBI GATnn'nnATC
Cuts at: 6 817 2127 4164 6
Size: 811 1310 2037 455
Fragments arranged by size:
2037 1310 811 455
BsaHI Gr'CG_yC
Cuts at: 2044 2292 2994 4490 2044
Size: 248 702 1496 2167
Fragments arranged by size:
2167 1496 702 248
BsaWI w'CCGG_w
Cuts at: 513 644 1096 1235 1802 2323 3001 3275 3422
Size: 131 452 139 567 521 678 274 147
Cuts at: 3422 3833 513
Size: 411 1293
Fragments arranged by size:
1293 678 567 521 452 411 274 147
139 131
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 399 697 916 1115 1225 399
Size: 298 219 199 110 3787
Fragments arranged by size:
3787 298 219 199 110
BseYI C'CCAG_C
Cuts at: 128 575 1152 1254 2038 2796 3373 3869 128
Size: 447 577 102 784 758 577 496 872
Fragments arranged by size:
872 784 758 577 577 496 447 102
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1092 1092
Size: 4613
BsiEI CG_ry'CG
Cuts at: 104 2155 2201 3409 104
Size: 2051 46 1208 1308
Fragments arranged by size:
2051 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1136 1627 2405 2595 3387 1136
Size: 491 778 190 792 2362
Fragments arranged by size:
2362 792 778 491 190
BsmI GAATG_Cn'
Cuts at: 312 389 312
Size: 77 4536
BsmBI CGTCTCn'nnnn_
Cuts at: 685 685
Size: 4613
Bsp1286I G_dGCh'C
Cuts at: 989 1128 1136 1627 2238 2331 2405 2595 2657
Size: 139 8 491 611 93 74 190 62
Cuts at: 2657 3387 989
Size: 730 2215
Fragments arranged by size:
2215 730 611 491 190 139 93 74
62 8
BspEI T'CCGG_A
Cuts at: 513 3833 513
Size: 3320 1293
BspHI T'CATG_A
Cuts at: 3789 3789
Size: 4613
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1325 1663 1877 2179 2560 1325
Size: 338 214 302 381 3378
Fragments arranged by size:
3378 381 338 302 214
BsrBI CCG'CTC
Cuts at: 50 2905 2959 4443 4608 50
Size: 2855 54 1484 165 55
Fragments arranged by size:
2855 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2525 2525
Size: 4613
BsrFI r'CCGG_y
Cuts at: 2611 2792 3001 2611
Size: 181 209 4223
Fragments arranged by size:
4223 209 181
BssSI C'ACGA_G
Cuts at: 2884 3242 2884
Size: 358 4255
BstAPI GCAn_nnn'nTGC
Cuts at: 546 1336 1424 1990 546
Size: 790 88 566 3169
Fragments arranged by size:
3169 790 566 88
BstBI TT'CG_AA
Cuts at: 2974 2974
Size: 4613
BstXI CCAn_nnnn'nTGG
Cuts at: 715 715
Size: 4613
BstYI r'GATC_y
Cuts at: 1 178 268 390 404 930 1238 1638 2105
Size: 177 90 122 14 526 308 400 467
Cuts at: 2105 2128 2463 2709 3721 3807 3819 4165 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 526 467 449 400 346 335 308
246 177 122 90 86 23 14 12
BtgI C'CryG_G
Cuts at: 107 587 759 770 853 107
Size: 480 172 11 83 3867
Fragments arranged by size:
3867 480 172 83 11
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 825 1035 2563 2744 2907 825
Size: 210 1528 181 163 2531
Fragments arranged by size:
2531 1528 210 181 163
BtsI GCAGTG_nn'
Cuts at: 1856 1856
Size: 4613
DraI TTT'AAA
Cuts at: 1612 1612
Size: 4613
DrdI GACnn_nn'nnGTC
Cuts at: 191 2319 3177 191
Size: 2128 858 1627
Fragments arranged by size:
2128 1627 858
EaeI y'GGCC_r
Cuts at: 968 2198 2372 2763 2790 4135 968
Size: 1230 174 391 27 1345 1446
Fragments arranged by size:
1446 1345 1230 391 174 27
EagI C'GGCC_G
Cuts at: 2198 2198
Size: 4613
EarI CTCTTCn'nnn_
Cuts at: 335 869 914 2636 2846 335
Size: 534 45 1722 210 2102
Fragments arranged by size:
2102 1722 534 210 45
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2821 3131 3277 4423 2821
Size: 310 146 1146 3011
Fragments arranged by size:
3011 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1625 1625
Size: 4613
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 709 1155 1357 1530 2438 2870 3617 3885 709
Size: 446 202 173 908 432 747 268 1437
Fragments arranged by size:
1437 908 747 446 432 268 202 173
EcoO109I rG'GnC_Cy
Cuts at: 262 711 1140 1260 1314 1347 1752 262
Size: 449 429 120 54 33 405 3123
Fragments arranged by size:
3123 449 429 405 120 54 33
EcoRI G'AATT_C
Cuts at: 1617 1617
Size: 4613
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 648 2632 648
Size: 1984 2629
FspI TGC'GCA
Cuts at: 2394 2394
Size: 4613
HaeII r_GCGC'y
Cuts at: 135 2047 2295 3317 4296 135
Size: 1912 248 1022 979 452
Fragments arranged by size:
1912 1022 979 452 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 482 514 2455 2485 2487 2517 482
Size: 32 1941 30 2 30 2578
Fragments arranged by size:
2578 1941 32 30 30 2
HincII GTy'rAC
Cuts at: 213 486 1652 213
Size: 273 1166 3174
Fragments arranged by size:
3174 1166 273
KasI G'GCGC_C
Cuts at: 2043 2291 2043
Size: 248 4365
KpnI G_GTAC'C
Cuts at: 645 1386 1633 645
Size: 741 247 3625
Fragments arranged by size:
3625 741 247
MluI A'CGCG_T
Cuts at: 4046 4046
Size: 4613
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 480 3284 3468 480
Size: 2804 184 1625
Fragments arranged by size:
2804 1625 184
MscI TGG'CCA
Cuts at: 2374 2374
Size: 4613
MslI CAynn'nnrTG
Cuts at: 586 634 2602 2729 586
Size: 48 1968 127 2470
Fragments arranged by size:
2470 1968 127 48
MspA1I CmG'CkG
Cuts at: 291 911 1724 2038 2398 3411 3656 291
Size: 620 813 314 360 1013 245 1248
Fragments arranged by size:
1248 1013 813 620 360 314 245
NaeI GCC'GGC
Cuts at: 2794 2794
Size: 4613
NarI GG'CG_CC
Cuts at: 2044 2292 2044
Size: 248 4365
NcoI C'CATG_G
Cuts at: 107 587 759 770 107
Size: 480 172 11 3950
Fragments arranged by size:
3950 480 172 11
NgoMIV G'CCGG_C
Cuts at: 2792 2792
Size: 4613
NspI r_CATG'y
Cuts at: 480 1666 2166 3073 480
Size: 1186 500 907 2020
Fragments arranged by size:
2020 1186 907 500
PasI CC'CwG_GG
Cuts at: 259 259
Size: 4613
PciI A'CATG_T
Cuts at: 3069 3069
Size: 4613
PflMI CCAn_nnn'nTGG
Cuts at: 135 135
Size: 4613
PmeI GTTT'AAAC
Cuts at: 1612 1612
Size: 4613
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3752 3784 3752
Size: 32 4581
PpuMI rG'GwC_Cy
Cuts at: 1260 1314 1260
Size: 54 4559
PshAI GACnn'nnGTC
Cuts at: 253 253
Size: 4613
PspOMI G'GGCC_C
Cuts at: 1124 1124
Size: 4613
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1375 1407 1375
Size: 32 4581
PstI C_TGCA'G
Cuts at: 145 204 1660 2345 145
Size: 59 1456 685 2413
Fragments arranged by size:
2413 1456 685 59
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4613
PvuII CAG'CTG
Cuts at: 291 2038 2398 291
Size: 1747 360 2506
Fragments arranged by size:
2506 1747 360
RsrII CG'GwC_CG
Cuts at: 2808 2808
Size: 4613
SacI G_AGCT'C
Cuts at: 1627 1627
Size: 4613
SalI G'TCGA_C
Cuts at: 211 1650 211
Size: 1439 3174
SanDI GG'GwC_CC
Cuts at: 1260 1260
Size: 4613
SapI GCTCTTCn'nnn_
Cuts at: 2636 2846 2636
Size: 210 4403
SbfI CC_TGCA'GG
Cuts at: 1660 1660
Size: 4613
ScaI AGT'ACT
Cuts at: 4130 4130
Size: 4613
SexAI A'CCwGG_T
Cuts at: 359 359
Size: 4613
SfcI C'TryA_G
Cuts at: 33 141 200 729 1656 2341 3334 3525 33
Size: 108 59 529 927 685 993 191 1121
Fragments arranged by size:
1121 993 927 685 529 191 108 59
SfoI GGC'GCC
Cuts at: 2045 2293 2045
Size: 248 4365
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4613
SmaI CCC'GGG
Cuts at: 1635 1635
Size: 4613
SmlI C'TyrA_G
Cuts at: 1768 2683 3175 3437 1768
Size: 915 492 262 2944
Fragments arranged by size:
2944 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4613
SphI G_CATG'C
Cuts at: 1666 2166 1666
Size: 500 4113
SspI AAT'ATT
Cuts at: 1578 1578
Size: 4613
StuI AGG'CCT
Cuts at: 1221 1221
Size: 4613
StyI C'CwwG_G
Cuts at: 107 587 759 770 1747 2101 107
Size: 480 172 11 977 354 2619
Fragments arranged by size:
2619 977 480 354 172 11
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1489 2196 2329 2788 2997 1489
Size: 707 133 459 209 3105
Fragments arranged by size:
3105 707 459 209 133
TatI w'GTAC_w
Cuts at: 4128 4128
Size: 4613
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4130 4162 4130
Size: 32 4581
Tth111I GACn'n_nGTC
Cuts at: 2410 2410
Size: 4613
XbaI T'CTAG_A
Cuts at: 1644 1644
Size: 4613
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1000 2108 1000
Size: 1108 3505
XmaI C'CCGG_G
Cuts at: 1633 1633
Size: 4613
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PspOMI PsrI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI AscI
AvrII BaeI BcgI BfrBI BmtI BsaXI BsiWI BsrGI
BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PfoI PmlI PsiI PspXI
SacII SfiI SgrAI SnaBI SrfI SwaI XhoI XmnI
ZraI