Restriction map for
pF1KB3590
(Circular) (Six-base) MAPSORT of: pF1KB3590.seq Check: 2562 from: 1 to: 3506
pF1KB3590 3506 bp
With 182 enzymes: SgfI *
April 24, 2009 13:02 ..
AccI GT'mk_AC
Cuts at: 544 544
Size: 3506
Acc65I G'GTAC_C
Cuts at: 522 522
Size: 3506
AclI AA'CG_TT
Cuts at: 3346 3346
Size: 3506
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 235 1331 1763 2510 2778 235
Size: 1096 432 747 268 963
Fragments arranged by size:
1096 963 747 432 268
AflIII A'CryG_T
Cuts at: 858 1962 2939 858
Size: 1104 977 1425
Fragments arranged by size:
1425 1104 977
AgeI A'CCGG_T
Cuts at: 1894 1894
Size: 3506
AlwNI CAG_nnn'CTG
Cuts at: 383 811 2378 383
Size: 428 1567 1511
Fragments arranged by size:
1567 1511 428
ApaLI G'TGCA_C
Cuts at: 2276 2276
Size: 3506
ApoI r'AATT_y
Cuts at: 510 2859 3115 510
Size: 2349 256 901
Fragments arranged by size:
2349 901 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3506
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3506
AvaI C'yCGr_G
Cuts at: 526 526
Size: 3506
BamHI G'GATC_C
Cuts at: 531 531
Size: 3506
BanI G'GyrC_C
Cuts at: 451 522 936 1184 1219 451
Size: 71 414 248 35 2738
Fragments arranged by size:
2738 414 248 71 35
BanII G_rGCy'C
Cuts at: 520 1550 520
Size: 1030 2476
BbeI G_GCGC'C
Cuts at: 940 1188 940
Size: 248 3258
BciVI GTATCCnnnnn_n'
Cuts at: 1404 2165 3506 1404
Size: 761 1341 1404
Fragments arranged by size:
1404 1341 761
BclI T'GATC_A
Cuts at: 1026 1026
Size: 3506
BglII A'GATC_T
Cuts at: 113 1021 3058 113
Size: 908 2037 561
Fragments arranged by size:
2037 908 561
BlpI GC'TnA_GC
Cuts at: 466 618 466
Size: 152 3354
Bme1580I G_kGCm'C
Cuts at: 1131 1224 2280 1131
Size: 93 1056 2357
Fragments arranged by size:
2357 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 718 799 1134 3278 718
Size: 81 335 2144 946
Fragments arranged by size:
2144 946 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1873 1873
Size: 3506
Bpu10I CC'TnA_GC
Cuts at: 307 307
Size: 3506
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 682 1561 2053 2351 682
Size: 879 492 298 1837
Fragments arranged by size:
1837 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3210 3210
Size: 3506
BsaAI yAC'GTr
Cuts at: 859 859
Size: 3506
BsaBI GATnn'nnATC
Cuts at: 6 1020 3057 6
Size: 1014 2037 455
Fragments arranged by size:
2037 1014 455
BsaHI Gr'CG_yC
Cuts at: 347 937 1185 1887 3383 347
Size: 590 248 702 1496 470
Fragments arranged by size:
1496 702 590 470 248
BsaWI w'CCGG_w
Cuts at: 695 1216 1894 2168 2315 2726 695
Size: 521 678 274 147 411 1475
Fragments arranged by size:
1475 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 447 931 1689 2266 2762 447
Size: 484 758 577 496 1191
Fragments arranged by size:
1191 758 577 496 484
BsiEI CG_ry'CG
Cuts at: 104 416 1048 1094 2302 104
Size: 312 632 46 1208 1308
Fragments arranged by size:
1308 1208 632 312 46
BsiHKAI G_wGCw'C
Cuts at: 520 1298 1488 2280 520
Size: 778 190 792 1746
Fragments arranged by size:
1746 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 520 1131 1224 1298 1488 1550 2280 520
Size: 611 93 74 190 62 730 1746
Fragments arranged by size:
1746 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2726 2726
Size: 3506
BspHI T'CATG_A
Cuts at: 2682 2682
Size: 3506
BspMI ACCTGCnnnn'nnnn_
Cuts at: 556 770 1072 1453 556
Size: 214 302 381 2609
Fragments arranged by size:
2609 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1798 1852 3336 3501 50
Size: 1748 54 1484 165 55
Fragments arranged by size:
1748 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1418 1418
Size: 3506
BsrFI r'CCGG_y
Cuts at: 271 477 1504 1685 1894 271
Size: 206 1027 181 209 1883
Fragments arranged by size:
1883 1027 209 206 181
BssSI C'ACGA_G
Cuts at: 286 1777 2135 286
Size: 1491 358 1657
Fragments arranged by size:
1657 1491 358
BstAPI GCAn_nnn'nTGC
Cuts at: 380 883 380
Size: 503 3003
BstBI TT'CG_AA
Cuts at: 1867 1867
Size: 3506
BstYI r'GATC_y
Cuts at: 1 113 531 998 1021 1356 1602 2614 2700
Size: 112 418 467 23 335 246 1012 86
Cuts at: 2700 2712 3058 1
Size: 12 346 449
Fragments arranged by size:
1012 467 449 418 346 335 246 112
86 23 12
BtgI C'CryG_G
Cuts at: 368 368
Size: 3506
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1456 1637 1800 1456
Size: 181 163 3162
Fragments arranged by size:
3162 181 163
BtsI GCAGTG_nn'
Cuts at: 749 749
Size: 3506
DraI TTT'AAA
Cuts at: 505 505
Size: 3506
DrdI GACnn_nn'nnGTC
Cuts at: 1212 2070 1212
Size: 858 2648
EaeI y'GGCC_r
Cuts at: 297 413 1091 1265 1656 1683 3028 297
Size: 116 678 174 391 27 1345 775
Fragments arranged by size:
1345 775 678 391 174 116 27
EagI C'GGCC_G
Cuts at: 413 1091 413
Size: 678 2828
EarI CTCTTCn'nnn_
Cuts at: 190 1529 1739 190
Size: 1339 210 1957
Fragments arranged by size:
1957 1339 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1714 2024 2170 3316 1714
Size: 310 146 1146 1904
Fragments arranged by size:
1904 1146 310 146
EcoICRI GAG'CTC
Cuts at: 518 518
Size: 3506
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 235 1331 1763 2510 2778 235
Size: 1096 432 747 268 963
Fragments arranged by size:
1096 963 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 645 645
Size: 3506
EcoRI G'AATT_C
Cuts at: 510 510
Size: 3506
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 135 1525 135
Size: 1390 2116
FspI TGC'GCA
Cuts at: 1287 1287
Size: 3506
HaeII r_GCGC'y
Cuts at: 940 1188 2210 3189 940
Size: 248 1022 979 1257
Fragments arranged by size:
1257 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1348 1378 1380 1410 1348
Size: 30 2 30 3444
Fragments arranged by size:
3444 30 30 2
HincII GTy'rAC
Cuts at: 545 545
Size: 3506
KasI G'GCGC_C
Cuts at: 936 1184 936
Size: 248 3258
KpnI G_GTAC'C
Cuts at: 526 526
Size: 3506
MluI A'CGCG_T
Cuts at: 2939 2939
Size: 3506
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 257 2177 2361 257
Size: 1920 184 1402
Fragments arranged by size:
1920 1402 184
MscI TGG'CCA
Cuts at: 299 1267 299
Size: 968 2538
MslI CAynn'nnrTG
Cuts at: 1495 1622 1495
Size: 127 3379
MspA1I CmG'CkG
Cuts at: 380 617 931 1291 2304 2549 380
Size: 237 314 360 1013 245 1337
Fragments arranged by size:
1337 1013 360 314 245 237
NaeI GCC'GGC
Cuts at: 273 479 1687 273
Size: 206 1208 2092
Fragments arranged by size:
2092 1208 206
NarI GG'CG_CC
Cuts at: 937 1185 937
Size: 248 3258
NcoI C'CATG_G
Cuts at: 368 368
Size: 3506
NgoMIV G'CCGG_C
Cuts at: 271 477 1685 271
Size: 206 1208 2092
Fragments arranged by size:
2092 1208 206
NspI r_CATG'y
Cuts at: 559 1059 1966 559
Size: 500 907 2099
Fragments arranged by size:
2099 907 500
PciI A'CATG_T
Cuts at: 1962 1962
Size: 3506
PmeI GTTT'AAAC
Cuts at: 505 505
Size: 3506
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2645 2677 2645
Size: 32 3474
PstI C_TGCA'G
Cuts at: 553 1238 553
Size: 685 2821
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3506
PvuII CAG'CTG
Cuts at: 380 931 1291 380
Size: 551 360 2595
Fragments arranged by size:
2595 551 360
RsrII CG'GwC_CG
Cuts at: 1701 1701
Size: 3506
SacI G_AGCT'C
Cuts at: 520 520
Size: 3506
SalI G'TCGA_C
Cuts at: 543 543
Size: 3506
SapI GCTCTTCn'nnn_
Cuts at: 190 1529 1739 190
Size: 1339 210 1957
Fragments arranged by size:
1957 1339 210
SbfI CC_TGCA'GG
Cuts at: 553 553
Size: 3506
ScaI AGT'ACT
Cuts at: 3023 3023
Size: 3506
SfcI C'TryA_G
Cuts at: 33 549 1234 2227 2418 33
Size: 516 685 993 191 1121
Fragments arranged by size:
1121 993 685 516 191
SfoI GGC'GCC
Cuts at: 938 1186 938
Size: 248 3258
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3506
SmaI CCC'GGG
Cuts at: 528 528
Size: 3506
SmlI C'TyrA_G
Cuts at: 661 1576 2068 2330 661
Size: 915 492 262 1837
Fragments arranged by size:
1837 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3506
SphI G_CATG'C
Cuts at: 559 1059 559
Size: 500 3006
StyI C'CwwG_G
Cuts at: 368 640 994 368
Size: 272 354 2880
Fragments arranged by size:
2880 354 272
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 136 439 1089 1222 1681 1890 136
Size: 303 650 133 459 209 1752
Fragments arranged by size:
1752 650 459 303 209 133
TatI w'GTAC_w
Cuts at: 206 3021 206
Size: 2815 691
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3023 3055 3023
Size: 32 3474
Tth111I GACn'n_nGTC
Cuts at: 258 1303 258
Size: 1045 2461
XbaI T'CTAG_A
Cuts at: 537 537
Size: 3506
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1001 1001
Size: 3506
XmaI C'CCGG_G
Cuts at: 526 526
Size: 3506
XmnI GAAnn'nnTTC
Cuts at: 115 115
Size: 3506
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglII BlpI Bme1580I BmrI BplI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PmeI PpiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BglI BmgBI BmtI BpmI BsaXI BseRI BsgI BsiWI
BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI ZraI