Restriction map for
pF1KB3552
(Circular) (Six-base) MAPSORT of: pF1KB3552.seq Check: 6589 from: 1 to: 4232
pF1KB3552 4232 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 202 331 202
Size: 129 4103
AccI GT'mk_AC
Cuts at: 1270 1270
Size: 4232
Acc65I G'GTAC_C
Cuts at: 1248 1248
Size: 4232
AclI AA'CG_TT
Cuts at: 4072 4072
Size: 4232
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 157 2057 2489 3236 3504 157
Size: 1900 432 747 268 885
Fragments arranged by size:
1900 885 747 432 268
AflIII A'CryG_T
Cuts at: 1584 2688 3665 1584
Size: 1104 977 2151
Fragments arranged by size:
2151 1104 977
AgeI A'CCGG_T
Cuts at: 2620 2620
Size: 4232
AleI CACnn'nnGTG
Cuts at: 297 297
Size: 4232
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 586 617 586
Size: 31 4201
AlwNI CAG_nnn'CTG
Cuts at: 288 735 840 945 1183 1224 1537 3104 288
Size: 447 105 105 238 41 313 1567 1416
Fragments arranged by size:
1567 1416 447 313 238 105 105 41
ApaI G_GGCC'C
Cuts at: 374 797 903 1045 1063 374
Size: 423 106 142 18 3543
Fragments arranged by size:
3543 423 142 106 18
ApaLI G'TGCA_C
Cuts at: 3002 3002
Size: 4232
ApoI r'AATT_y
Cuts at: 1236 3585 3841 1236
Size: 2349 256 1627
Fragments arranged by size:
2349 1627 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4232
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4232
AvaI C'yCGr_G
Cuts at: 496 659 1136 1252 496
Size: 163 477 116 3476
Fragments arranged by size:
3476 477 163 116
AvrII C'CTAG_G
Cuts at: 1129 1129
Size: 4232
BamHI G'GATC_C
Cuts at: 1257 1257
Size: 4232
BanI G'GyrC_C
Cuts at: 310 1120 1248 1662 1910 1945 310
Size: 810 128 414 248 35 2597
Fragments arranged by size:
2597 810 414 248 128 35
BanII G_rGCy'C
Cuts at: 374 479 587 797 903 968 1045 1063 1246
Size: 105 108 210 106 65 77 18 183
Cuts at: 1246 2276 374
Size: 1030 2330
Fragments arranged by size:
2330 1030 210 183 108 106 105 77
65 18
BbeI G_GCGC'C
Cuts at: 314 1666 1914 314
Size: 1352 248 2632
Fragments arranged by size:
2632 1352 248
BbvCI CC'TCA_GC
Cuts at: 605 605
Size: 4232
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 247 281 247
Size: 34 4198
BciVI GTATCCnnnnn_n'
Cuts at: 2130 2891 4232 2130
Size: 761 1341 2130
Fragments arranged by size:
2130 1341 761
BclI T'GATC_A
Cuts at: 1752 1752
Size: 4232
BglI GCCn_nnn'nGGC
Cuts at: 239 760 907 928 1029 1049 239
Size: 521 147 21 101 20 3422
Fragments arranged by size:
3422 521 147 101 21 20
BglII A'GATC_T
Cuts at: 1747 3784 1747
Size: 2037 2195
BlpI GC'TnA_GC
Cuts at: 710 764 1344 710
Size: 54 580 3598
Fragments arranged by size:
3598 580 54
Bme1580I G_kGCm'C
Cuts at: 374 756 797 903 1045 1063 1125 1857 1950
Size: 382 41 106 142 18 62 732 93
Cuts at: 1950 3006 374
Size: 1056 1600
Fragments arranged by size:
1600 1056 732 382 142 106 93 62
41 18
BmrI ACTGGGnnnn_n'
Cuts at: 1444 1525 1860 4004 1444
Size: 81 335 2144 1672
Fragments arranged by size:
2144 1672 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2599 2599
Size: 4232
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 601 778 925 967 1055 1186 601
Size: 177 147 42 88 131 3647
Fragments arranged by size:
3647 177 147 131 88 42
Bpu10I CC'TnA_GC
Cuts at: 586 605 743 932 938 586
Size: 19 138 189 6 3880
Fragments arranged by size:
3880 189 138 19 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1408 2287 2779 3077 1408
Size: 879 492 298 2563
Fragments arranged by size:
2563 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 183 3936 183
Size: 3753 479
BsaAI yAC'GTr
Cuts at: 1585 1585
Size: 4232
BsaBI GATnn'nnATC
Cuts at: 6 1746 3783 6
Size: 1740 2037 455
Fragments arranged by size:
2037 1740 455
BsaHI Gr'CG_yC
Cuts at: 311 1663 1911 2613 4109 311
Size: 1352 248 702 1496 434
Fragments arranged by size:
1496 1352 702 434 248
BsaWI w'CCGG_w
Cuts at: 1421 1942 2620 2894 3041 3452 1421
Size: 521 678 274 147 411 2201
Fragments arranged by size:
2201 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 268 298 268
Size: 30 4202
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 384 622 964 1096 384
Size: 238 342 132 3520
Fragments arranged by size:
3520 342 238 132
BseYI C'CCAG_C
Cuts at: 373 382 465 508 612 1124 1211 1657 2415
Size: 9 83 43 104 512 87 446 758
Cuts at: 2415 2992 3488 373
Size: 577 496 1117
Fragments arranged by size:
1117 758 577 512 496 446 104 87
83 43 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 277 546 613 814 892 277
Size: 269 67 201 78 3617
Fragments arranged by size:
3617 269 201 78 67
BsiEI CG_ry'CG
Cuts at: 104 1774 1820 3028 104
Size: 1670 46 1208 1308
Fragments arranged by size:
1670 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 587 968 1246 2024 2214 3006 587
Size: 381 278 778 190 792 1813
Fragments arranged by size:
1813 792 778 381 278 190
BsmI GAATG_Cn'
Cuts at: 606 606
Size: 4232
Bsp1286I G_dGCh'C
Cuts at: 374 479 587 756 797 903 968 1045 1063
Size: 105 108 169 41 106 65 77 18
Cuts at: 1063 1125 1246 1857 1950 2024 2214 2276 3006
Size: 62 121 611 93 74 190 62 730
Cuts at: 3006 374
Size: 1600
Fragments arranged by size:
1600 730 611 190 169 121 108 106
105 93 77 74 65 62 62 41
18
BspEI T'CCGG_A
Cuts at: 3452 3452
Size: 4232
BspHI T'CATG_A
Cuts at: 3408 3408
Size: 4232
BspMI ACCTGCnnnn'nnnn_
Cuts at: 202 331 578 989 1282 1496 1798 2179 202
Size: 129 247 411 293 214 302 381 2255
Fragments arranged by size:
2255 411 381 302 293 247 214 129
BsrBI CCG'CTC
Cuts at: 50 279 855 2524 2578 4062 4227 50
Size: 229 576 1669 54 1484 165 55
Fragments arranged by size:
1669 1484 576 229 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2144 2144
Size: 4232
BsrFI r'CCGG_y
Cuts at: 2230 2411 2620 2230
Size: 181 209 3842
Fragments arranged by size:
3842 209 181
BssSI C'ACGA_G
Cuts at: 2503 2861 2503
Size: 358 3874
BstAPI GCAn_nnn'nTGC
Cuts at: 288 642 697 1183 1609 288
Size: 354 55 486 426 2911
Fragments arranged by size:
2911 486 426 354 55
BstBI TT'CG_AA
Cuts at: 2593 2593
Size: 4232
BstXI CCAn_nnnn'nTGG
Cuts at: 114 114
Size: 4232
BstYI r'GATC_y
Cuts at: 1 1257 1724 1747 2082 2328 3340 3426 3438
Size: 1256 467 23 335 246 1012 86 12
Cuts at: 3438 3784 1
Size: 346 449
Fragments arranged by size:
1256 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 358 358
Size: 4232
BtgI C'CryG_G
Cuts at: 128 454 1027 128
Size: 326 573 3333
Fragments arranged by size:
3333 573 326
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 195 356 2182 2363 2526 195
Size: 161 1826 181 163 1901
Fragments arranged by size:
1901 1826 181 163 161
BtsI GCAGTG_nn'
Cuts at: 704 1475 704
Size: 771 3461
DraI TTT'AAA
Cuts at: 1231 1231
Size: 4232
DraIII CAC_nnn'GTG
Cuts at: 697 697
Size: 4232
DrdI GACnn_nn'nnGTC
Cuts at: 1938 2796 1938
Size: 858 3374
EaeI y'GGCC_r
Cuts at: 125 240 701 1817 1991 2382 2409 3754 125
Size: 115 461 1116 174 391 27 1345 603
Fragments arranged by size:
1345 1116 603 461 391 174 115 27
EagI C'GGCC_G
Cuts at: 1817 1817
Size: 4232
EarI CTCTTCn'nnn_
Cuts at: 2255 2465 2255
Size: 210 4022
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2440 2750 2896 4042 2440
Size: 310 146 1146 2630
Fragments arranged by size:
2630 1146 310 146
EcoICRI GAG'CTC
Cuts at: 585 966 1244 585
Size: 381 278 3573
Fragments arranged by size:
3573 381 278
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 157 601 778 925 967 1055 1186 2057 2489
Size: 444 177 147 42 88 131 871 432
Cuts at: 2489 3236 3504 157
Size: 747 268 885
Fragments arranged by size:
885 871 747 444 432 268 177 147
131 88 42
EcoNI CCTnn'n_nnAGG
Cuts at: 832 832
Size: 4232
EcoO109I rG'GnC_Cy
Cuts at: 370 389 576 732 786 794 837 900 1041
Size: 19 187 156 54 8 43 63 141
Cuts at: 1041 1042 1059 1060 1133 1371 370
Size: 1 17 1 73 238 3231
Fragments arranged by size:
3231 238 187 156 141 73 63 54
43 19 17 8 1 1
EcoRI G'AATT_C
Cuts at: 1236 1236
Size: 4232
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2251 2251
Size: 4232
FspI TGC'GCA
Cuts at: 2013 2013
Size: 4232
HaeII r_GCGC'y
Cuts at: 314 828 1100 1666 1914 2936 3915 314
Size: 514 272 566 248 1022 979 631
Fragments arranged by size:
1022 979 631 566 514 272 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2074 2104 2106 2136 2074
Size: 30 2 30 4170
Fragments arranged by size:
4170 30 30 2
HincII GTy'rAC
Cuts at: 1271 1271
Size: 4232
KasI G'GCGC_C
Cuts at: 310 1662 1910 310
Size: 1352 248 2632
Fragments arranged by size:
2632 1352 248
KpnI G_GTAC'C
Cuts at: 1252 1252
Size: 4232
MluI A'CGCG_T
Cuts at: 3665 3665
Size: 4232
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2903 3087 2903
Size: 184 4048
MscI TGG'CCA
Cuts at: 1993 1993
Size: 4232
MslI CAynn'nnrTG
Cuts at: 297 1118 2221 2348 297
Size: 821 1103 127 2181
Fragments arranged by size:
2181 1103 821 127
MspA1I CmG'CkG
Cuts at: 261 404 774 781 1343 1657 2017 3030 3275
Size: 143 370 7 562 314 360 1013 245
Cuts at: 3275 261
Size: 1218
Fragments arranged by size:
1218 1013 562 370 360 314 245 143
7
NaeI GCC'GGC
Cuts at: 2413 2413
Size: 4232
NarI GG'CG_CC
Cuts at: 311 1663 1911 311
Size: 1352 248 2632
Fragments arranged by size:
2632 1352 248
NcoI C'CATG_G
Cuts at: 128 128
Size: 4232
NgoMIV G'CCGG_C
Cuts at: 2411 2411
Size: 4232
NspI r_CATG'y
Cuts at: 718 1285 1785 2692 718
Size: 567 500 907 2258
Fragments arranged by size:
2258 907 567 500
PasI CC'CwG_GG
Cuts at: 523 790 523
Size: 267 3965
PciI A'CATG_T
Cuts at: 2688 2688
Size: 4232
PflMI CCAn_nnn'nTGG
Cuts at: 165 204 840 1224 165
Size: 39 636 384 3173
Fragments arranged by size:
3173 636 384 39
PfoI T'CCnGG_A
Cuts at: 1163 1163
Size: 4232
PmeI GTTT'AAAC
Cuts at: 1231 1231
Size: 4232
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3371 3403 3371
Size: 32 4200
PpuMI rG'GwC_Cy
Cuts at: 837 837
Size: 4232
PshAI GACnn'nnGTC
Cuts at: 404 404
Size: 4232
PspOMI G'GGCC_C
Cuts at: 370 793 899 1041 1059 370
Size: 423 106 142 18 3543
Fragments arranged by size:
3543 423 142 106 18
PstI C_TGCA'G
Cuts at: 689 731 773 986 1279 1964 689
Size: 42 42 213 293 685 2957
Fragments arranged by size:
2957 685 293 213 42 42
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4232
PvuII CAG'CTG
Cuts at: 404 774 1657 2017 404
Size: 370 883 360 2619
Fragments arranged by size:
2619 883 370 360
RsrII CG'GwC_CG
Cuts at: 2427 2427
Size: 4232
SacI G_AGCT'C
Cuts at: 587 968 1246 587
Size: 381 278 3573
Fragments arranged by size:
3573 381 278
SalI G'TCGA_C
Cuts at: 1269 1269
Size: 4232
SapI GCTCTTCn'nnn_
Cuts at: 2255 2465 2255
Size: 210 4022
SbfI CC_TGCA'GG
Cuts at: 689 1279 689
Size: 590 3642
ScaI AGT'ACT
Cuts at: 3749 3749
Size: 4232
SfcI C'TryA_G
Cuts at: 33 685 727 769 982 1275 1960 2953 3144
Size: 652 42 42 213 293 685 993 191
Cuts at: 3144 33
Size: 1121
Fragments arranged by size:
1121 993 685 652 293 213 191 42
42
SfiI GGCCn_nnn'nGGCC
Cuts at: 239 239
Size: 4232
SfoI GGC'GCC
Cuts at: 312 1664 1912 312
Size: 1352 248 2632
Fragments arranged by size:
2632 1352 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4232
SmaI CCC'GGG
Cuts at: 661 1254 661
Size: 593 3639
SmlI C'TyrA_G
Cuts at: 1387 2302 2794 3056 1387
Size: 915 492 262 2563
Fragments arranged by size:
2563 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4232
SphI G_CATG'C
Cuts at: 718 1285 1785 718
Size: 567 500 3165
Fragments arranged by size:
3165 567 500
StuI AGG'CCT
Cuts at: 625 625
Size: 4232
StyI C'CwwG_G
Cuts at: 128 1129 1366 1720 128
Size: 1001 237 354 2640
Fragments arranged by size:
2640 1001 354 237
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 135 561 1815 1948 2407 2616 135
Size: 426 1254 133 459 209 1751
Fragments arranged by size:
1751 1254 459 426 209 133
TatI w'GTAC_w
Cuts at: 3747 3747
Size: 4232
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3749 3781 3749
Size: 32 4200
Tth111I GACn'n_nGTC
Cuts at: 2029 2029
Size: 4232
XbaI T'CTAG_A
Cuts at: 1263 1263
Size: 4232
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1727 1727
Size: 4232
XmaI C'CCGG_G
Cuts at: 659 1252 659
Size: 593 3639
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
AvrII BamHI BanI BanII BbeI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfiI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AloI AscI BaeI BbsI
BfrBI BmgBI BmtI BsiWI BsmBI BsrGI BssHII BstEII
BstZ17I ClaI CspCI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PmlI
PsiI PspXI PsrI SacII SanDI SexAI SgrAI SnaBI
SrfI SspI SwaI XhoI XmnI ZraI