Restriction map for
pF1KB3540
(Circular) (Six-base) MAPSORT of: pF1KB3540.seq Check: 5192 from: 1 to: 6638
pF1KB3540 6638 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AccI GT'mk_AC
Cuts at: 824 3025 3676 824
Size: 2201 651 3786
Fragments arranged by size:
3786 2201 651
Acc65I G'GTAC_C
Cuts at: 2123 3654 2123
Size: 1531 5107
AclI AA'CG_TT
Cuts at: 6478 6478
Size: 6638
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 498 1529 4463 4895 5248 5910 498
Size: 1031 2934 432 353 662 1226
Fragments arranged by size:
2934 1226 1031 662 432 353
AflIII A'CryG_T
Cuts at: 1880 3990 5790 6071 1880
Size: 2110 1800 281 2447
Fragments arranged by size:
2447 2110 1800 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1465 2229 3349 1465
Size: 764 1120 4754
Fragments arranged by size:
4754 1120 764
AlwNI CAG_nnn'CTG
Cuts at: 3120 3300 3943 5381 3120
Size: 180 643 1438 4377
Fragments arranged by size:
4377 1438 643 180
ApaLI G'TGCA_C
Cuts at: 5476 5476
Size: 6638
ApoI r'AATT_y
Cuts at: 124 596 878 1645 1651 1936 2098 2317 2626
Size: 472 282 767 6 285 162 219 309
Cuts at: 2626 2925 3110 3541 3642 5991 6247 124
Size: 299 185 431 101 2349 256 515
Fragments arranged by size:
2349 767 515 472 431 309 299 285
282 256 219 185 162 101 6
AseI AT'TA_AT
Cuts at: 20 20
Size: 6638
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6638
AvaI C'yCGr_G
Cuts at: 3658 3658
Size: 6638
BamHI G'GATC_C
Cuts at: 333 2462 3663 333
Size: 2129 1201 3308
Fragments arranged by size:
3308 2129 1201
BanI G'GyrC_C
Cuts at: 2123 2263 3654 4068 4316 4351 2123
Size: 140 1391 414 248 35 4410
Fragments arranged by size:
4410 1391 414 248 140 35
BanII G_rGCy'C
Cuts at: 710 2786 3652 4682 710
Size: 2076 866 1030 2666
Fragments arranged by size:
2666 2076 1030 866
BbeI G_GCGC'C
Cuts at: 4072 4320 4072
Size: 248 6390
BbsI GAAGACnn'nnnn_
Cuts at: 670 1011 1121 1214 1877 1927 2873 670
Size: 341 110 93 663 50 946 4435
Fragments arranged by size:
4435 946 663 341 110 93 50
BbvCI CC'TCA_GC
Cuts at: 3206 3235 3206
Size: 29 6609
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1098 1132 1948 1982 3558 3592 1098
Size: 34 816 34 1576 34 4144
Fragments arranged by size:
4144 1576 816 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4536 5592 6638 4536
Size: 1056 1046 4536
Fragments arranged by size:
4536 1056 1046
BclI T'GATC_A
Cuts at: 1811 3188 4158 1811
Size: 1377 970 4291
Fragments arranged by size:
4291 1377 970
BglI GCCn_nnn'nGGC
Cuts at: 907 907
Size: 6638
BglII A'GATC_T
Cuts at: 420 777 1383 4153 6190 420
Size: 357 606 2770 2037 868
Fragments arranged by size:
2770 2037 868 606 357
BlpI GC'TnA_GC
Cuts at: 3750 3750
Size: 6638
Bme1580I G_kGCm'C
Cuts at: 2705 4263 4356 5480 2705
Size: 1558 93 1124 3863
Fragments arranged by size:
3863 1558 1124 93
BmgBI CAC'GTC
Cuts at: 2700 2700
Size: 6638
BmrI ACTGGGnnnn_n'
Cuts at: 677 1009 2699 3850 3931 4266 6410 677
Size: 332 1690 1151 81 335 2144 905
Fragments arranged by size:
2144 1690 1151 905 335 332 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5005 5005
Size: 6638
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1577 1577
Size: 6638
Bpu10I CC'TnA_GC
Cuts at: 3206 3235 3206
Size: 29 6609
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 532 649 2436 2837 3814 4693 5407 5705 532
Size: 117 1787 401 977 879 714 298 1465
Fragments arranged by size:
1787 1465 977 879 714 401 298 117
BsaI GGTCTCn'nnnn_
Cuts at: 3218 6342 3218
Size: 3124 3514
BsaAI yAC'GTr
Cuts at: 2271 2311 3991 2271
Size: 40 1680 4918
Fragments arranged by size:
4918 1680 40
BsaBI GATnn'nnATC
Cuts at: 6 4152 6189 6
Size: 4146 2037 455
Fragments arranged by size:
4146 2037 455
BsaHI Gr'CG_yC
Cuts at: 4069 4317 5019 6515 4069
Size: 248 702 1496 4192
Fragments arranged by size:
4192 1496 702 248
BsaWI w'CCGG_w
Cuts at: 3827 4348 5026 5437 5584 5858 3827
Size: 521 678 411 147 274 4607
Fragments arranged by size:
4607 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1139 1169 1976 2006 2585 2615 1139
Size: 30 807 30 579 30 5162
Fragments arranged by size:
5162 807 579 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 202 1618 1642 3223 202
Size: 1416 24 1581 3617
Fragments arranged by size:
3617 1581 1416 24
BseYI C'CCAG_C
Cuts at: 1190 1572 1823 3293 4063 4821 5486 5894 1190
Size: 382 251 1470 770 758 665 408 1934
Fragments arranged by size:
1934 1470 770 758 665 408 382 251
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2242 2964 3214 2242
Size: 722 250 5666
Fragments arranged by size:
5666 722 250
BsiEI CG_ry'CG
Cuts at: 104 316 4180 4226 5456 104
Size: 212 3864 46 1230 1286
Fragments arranged by size:
3864 1286 1230 212 46
BsiHKAI G_wGCw'C
Cuts at: 2786 3342 3652 4430 4620 5480 2786
Size: 556 310 778 190 860 3944
Fragments arranged by size:
3944 860 778 556 310 190
BsmI GAATG_Cn'
Cuts at: 295 295
Size: 6638
Bsp1286I G_dGCh'C
Cuts at: 710 2705 2786 3342 3652 4263 4356 4430 4620
Size: 1995 81 556 310 611 93 74 190
Cuts at: 4620 4682 5480 710
Size: 62 798 1868
Fragments arranged by size:
1995 1868 798 611 556 310 190 93
81 74 62
BspHI T'CATG_A
Cuts at: 2144 3030 5070 2144
Size: 886 2040 3712
Fragments arranged by size:
3712 2040 886
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2214 3236 3688 3902 4204 4585 2214
Size: 1022 452 214 302 381 4267
Fragments arranged by size:
4267 1022 452 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4930 4984 6468 6633 50
Size: 4880 54 1484 165 55
Fragments arranged by size:
4880 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 484 917 1247 3059 4550 484
Size: 433 330 1812 1491 2572
Fragments arranged by size:
2572 1812 1491 433 330
BsrFI r'CCGG_y
Cuts at: 4636 4817 4636
Size: 181 6457
BsrGI T'GTAC_A
Cuts at: 1142 1246 1142
Size: 104 6534
BssSI C'ACGA_G
Cuts at: 3280 4909 5617 3280
Size: 1629 708 4301
Fragments arranged by size:
4301 1629 708
BstAPI GCAn_nnn'nTGC
Cuts at: 4015 4015
Size: 6638
BstBI TT'CG_AA
Cuts at: 2511 4999 2511
Size: 2488 4150
BstXI CCAn_nnnn'nTGG
Cuts at: 345 3504 345
Size: 3159 3479
BstYI r'GATC_y
Cuts at: 1 333 420 777 861 1383 1395 2462 2616
Size: 332 87 357 84 522 12 1067 154
Cuts at: 2616 3663 4130 4153 4488 4734 5040 5052 5138
Size: 1047 467 23 335 246 306 12 86
Cuts at: 5138 6190 1
Size: 1052 449
Fragments arranged by size:
1067 1052 1047 522 467 449 357 335
332 306 246 154 87 86 84 23
12 12
BtgI C'CryG_G
Cuts at: 107 2798 107
Size: 2691 3947
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2092 4588 4769 4932 2092
Size: 2496 181 163 3798
Fragments arranged by size:
3798 2496 181 163
BtsI GCAGTG_nn'
Cuts at: 417 1338 2976 3250 3881 417
Size: 921 1638 274 631 3174
Fragments arranged by size:
3174 1638 921 631 274
DraI TTT'AAA
Cuts at: 2903 2933 3637 2903
Size: 30 704 5904
Fragments arranged by size:
5904 704 30
DrdI GACnn_nn'nnGTC
Cuts at: 2410 4344 5688 2410
Size: 1934 1344 3360
Fragments arranged by size:
3360 1934 1344
EaeI y'GGCC_r
Cuts at: 110 2454 4223 4397 4788 4815 6160 110
Size: 2344 1769 174 391 27 1345 588
Fragments arranged by size:
2344 1769 1345 588 391 174 27
EagI C'GGCC_G
Cuts at: 4223 4223
Size: 6638
EarI CTCTTCn'nnn_
Cuts at: 226 1595 2363 4661 4871 226
Size: 1369 768 2298 210 1993
Fragments arranged by size:
2298 1993 1369 768 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 408 4846 5588 5734 6448 408
Size: 4438 742 146 714 598
Fragments arranged by size:
4438 742 714 598 146
EcoICRI GAG'CTC
Cuts at: 2784 3650 2784
Size: 866 5772
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 498 1529 1577 4463 4895 5248 5910 498
Size: 1031 48 2886 432 353 662 1226
Fragments arranged by size:
2886 1226 1031 662 432 353 48
EcoNI CCTnn'n_nnAGG
Cuts at: 1261 3046 1261
Size: 1785 4853
EcoO109I rG'GnC_Cy
Cuts at: 1719 3777 1719
Size: 2058 4580
EcoRI G'AATT_C
Cuts at: 1645 2317 3541 3642 1645
Size: 672 1224 101 4641
Fragments arranged by size:
4641 1224 672 101
EcoRV GAT'ATC
Cuts at: 991 1254 2494 2598 991
Size: 263 1240 104 5031
Fragments arranged by size:
5031 1240 263 104
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 669 2907 3544 4657 669
Size: 2238 637 1113 2650
Fragments arranged by size:
2650 2238 1113 637
FspI TGC'GCA
Cuts at: 4419 4419
Size: 6638
HaeII r_GCGC'y
Cuts at: 4072 4320 5550 6321 4072
Size: 248 1230 771 4389
Fragments arranged by size:
4389 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 178 210 744 776 1139 1171 1976 2008 2161
Size: 32 534 32 363 32 805 32 153
Cuts at: 2161 2193 2596 2628 3114 3121 3146 3153 4480
Size: 32 403 32 486 7 25 7 1327
Cuts at: 4480 4510 4512 4542 178
Size: 30 2 30 2274
Fragments arranged by size:
2274 1327 805 534 486 403 363 153
32 32 32 32 32 32 30 30
25 7 7 2
HincII GTy'rAC
Cuts at: 2991 3026 3677 2991
Size: 35 651 5952
Fragments arranged by size:
5952 651 35
HindIII A'AGCT_T
Cuts at: 1532 1532
Size: 6638
KasI G'GCGC_C
Cuts at: 4068 4316 4068
Size: 248 6390
KpnI G_GTAC'C
Cuts at: 2127 3658 2127
Size: 1531 5107
MfeI C'AATT_G
Cuts at: 1931 2083 1931
Size: 152 6486
MluI A'CGCG_T
Cuts at: 6071 6071
Size: 6638
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 345 5397 5581 345
Size: 5052 184 1402
Fragments arranged by size:
5052 1402 184
MscI TGG'CCA
Cuts at: 112 2456 4399 112
Size: 2344 1943 2351
Fragments arranged by size:
2351 2344 1943
MslI CAynn'nnrTG
Cuts at: 925 2335 4627 4754 925
Size: 1410 2292 127 2809
Fragments arranged by size:
2809 2292 1410 127
MspA1I CmG'CkG
Cuts at: 1527 3300 3749 4063 4423 5207 5452 1527
Size: 1773 449 314 360 784 245 2713
Fragments arranged by size:
2713 1773 784 449 360 314 245
NaeI GCC'GGC
Cuts at: 4819 4819
Size: 6638
NarI GG'CG_CC
Cuts at: 4069 4317 4069
Size: 248 6390
NcoI C'CATG_G
Cuts at: 107 107
Size: 6638
NdeI CA'TA_TG
Cuts at: 2574 2574
Size: 6638
NgoMIV G'CCGG_C
Cuts at: 4817 4817
Size: 6638
NspI r_CATG'y
Cuts at: 545 1884 1930 2013 2947 3691 4191 5794 545
Size: 1339 46 83 934 744 500 1603 1389
Fragments arranged by size:
1603 1389 1339 934 744 500 83 46
PciI A'CATG_T
Cuts at: 1880 5790 1880
Size: 3910 2728
PflMI CCAn_nnn'nTGG
Cuts at: 1050 1062 1050
Size: 12 6626
PmeI GTTT'AAAC
Cuts at: 3637 3637
Size: 6638
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5084 5116 5084
Size: 32 6606
PshAI GACnn'nnGTC
Cuts at: 1682 1682
Size: 6638
PsiI TTA'TAA
Cuts at: 1763 1877 1949 1763
Size: 114 72 6452
Fragments arranged by size:
6452 114 72
PstI C_TGCA'G
Cuts at: 412 1345 3685 4370 412
Size: 933 2340 685 2680
Fragments arranged by size:
2680 2340 933 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6638
PvuII CAG'CTG
Cuts at: 1527 3300 4063 4423 1527
Size: 1773 763 360 3742
Fragments arranged by size:
3742 1773 763 360
RsrII CG'GwC_CG
Cuts at: 4833 4833
Size: 6638
SacI G_AGCT'C
Cuts at: 2786 3652 2786
Size: 866 5772
SalI G'TCGA_C
Cuts at: 3024 3675 3024
Size: 651 5987
SapI GCTCTTCn'nnn_
Cuts at: 4661 4871 4661
Size: 210 6428
SbfI CC_TGCA'GG
Cuts at: 3685 3685
Size: 6638
ScaI AGT'ACT
Cuts at: 6155 6155
Size: 6638
SfcI C'TryA_G
Cuts at: 33 408 1341 1730 3681 4366 5334 5525 33
Size: 375 933 389 1951 685 968 191 1146
Fragments arranged by size:
1951 1146 968 933 685 389 375 191
SfoI GGC'GCC
Cuts at: 4070 4318 4070
Size: 248 6390
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6638
SmaI CCC'GGG
Cuts at: 3660 3660
Size: 6638
SmlI C'TyrA_G
Cuts at: 547 664 2415 2852 3793 4708 5422 5684 547
Size: 117 1751 437 941 915 714 262 1501
Fragments arranged by size:
1751 1501 941 915 714 437 262 117
SnaBI TAC'GTA
Cuts at: 2271 2311 2271
Size: 40 6598
SpeI A'CTAG_T
Cuts at: 67 2683 67
Size: 2616 4022
SphI G_CATG'C
Cuts at: 3691 4191 3691
Size: 500 6138
SspI AAT'ATT
Cuts at: 1375 1869 2559 1375
Size: 494 690 5454
Fragments arranged by size:
5454 690 494
StuI AGG'CCT
Cuts at: 491 962 491
Size: 471 6167
StyI C'CwwG_G
Cuts at: 107 1979 3772 4126 107
Size: 1872 1793 354 2619
Fragments arranged by size:
2619 1872 1793 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 302 1894 4221 4354 4813 5022 302
Size: 1592 2327 133 459 209 1918
Fragments arranged by size:
2327 1918 1592 459 209 133
TatI w'GTAC_w
Cuts at: 1142 1246 1358 6153 1142
Size: 104 112 4795 1627
Fragments arranged by size:
4795 1627 112 104
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 387 419 2585 2617 6155 6187 387
Size: 32 2166 32 3538 32 838
Fragments arranged by size:
3538 2166 838 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 4435 4435
Size: 6638
XbaI T'CTAG_A
Cuts at: 3537 3669 3537
Size: 132 6506
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2805 4133 2805
Size: 1328 5310
XmaI C'CCGG_G
Cuts at: 3658 3658
Size: 6638
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlfI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NspI PciI PflMI PmeI PpiI PshAI PsiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AloI
ApaI AscI AvrII BaeI BfrBI BmtI BsiWI BsmBI
BspEI BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII
FseI FspAI HpaI NheI NotI NruI NsiI PacI
PasI PfoI PmlI PpuMI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SrfI SwaI XhoI XmnI
ZraI