Restriction map for
pF1KB3512
(Circular) (Six-base) MAPSORT of: pF1KB3512.seq Check: 6259 from: 1 to: 4355
pF1KB3512 4355 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AccI GT'mk_AC
Cuts at: 1393 1393
Size: 4355
Acc65I G'GTAC_C
Cuts at: 1371 1371
Size: 4355
AclI AA'CG_TT
Cuts at: 4195 4195
Size: 4355
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2180 2612 2965 3627 2180
Size: 432 353 662 2908
Fragments arranged by size:
2908 662 432 353
AflII C'TTAA_G
Cuts at: 523 523
Size: 4355
AflIII A'CryG_T
Cuts at: 1707 3507 3788 1707
Size: 1800 281 2274
Fragments arranged by size:
2274 1800 281
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 854 886 854
Size: 32 4323
AlwNI CAG_nnn'CTG
Cuts at: 304 465 1660 3098 304
Size: 161 1195 1438 1561
Fragments arranged by size:
1561 1438 1195 161
ApaLI G'TGCA_C
Cuts at: 3193 3193
Size: 4355
ApoI r'AATT_y
Cuts at: 284 1359 3708 3964 284
Size: 1075 2349 256 675
Fragments arranged by size:
2349 1075 675 256
AseI AT'TA_AT
Cuts at: 20 1061 20
Size: 1041 3314
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4355
AvaI C'yCGr_G
Cuts at: 1375 1375
Size: 4355
BamHI G'GATC_C
Cuts at: 1380 1380
Size: 4355
BanI G'GyrC_C
Cuts at: 1371 1785 2033 2068 1371
Size: 414 248 35 3658
Fragments arranged by size:
3658 414 248 35
BanII G_rGCy'C
Cuts at: 152 1369 2399 152
Size: 1217 1030 2108
Fragments arranged by size:
2108 1217 1030
BbeI G_GCGC'C
Cuts at: 1789 2037 1789
Size: 248 4107
BbsI GAAGACnn'nnnn_
Cuts at: 176 759 1325 176
Size: 583 566 3206
Fragments arranged by size:
3206 583 566
BciVI GTATCCnnnnn_n'
Cuts at: 2253 3309 4355 2253
Size: 1056 1046 2253
Fragments arranged by size:
2253 1056 1046
BclI T'GATC_A
Cuts at: 816 851 1875 816
Size: 35 1024 3296
Fragments arranged by size:
3296 1024 35
BglI GCCn_nnn'nGGC
Cuts at: 1075 1087 1075
Size: 12 4343
BglII A'GATC_T
Cuts at: 519 1870 3907 519
Size: 1351 2037 967
Fragments arranged by size:
2037 1351 967
BlpI GC'TnA_GC
Cuts at: 1467 1467
Size: 4355
Bme1580I G_kGCm'C
Cuts at: 1980 2073 3197 1980
Size: 93 1124 3138
Fragments arranged by size:
3138 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 201 1567 1648 1983 4127 201
Size: 1366 81 335 2144 429
Fragments arranged by size:
2144 1366 429 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 756 2722 756
Size: 1966 2389
Bpu10I CC'TnA_GC
Cuts at: 893 893
Size: 4355
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1299 1531 2410 3124 3422 1299
Size: 232 879 714 298 2232
Fragments arranged by size:
2232 879 714 298 232
BsaI GGTCTCn'nnnn_
Cuts at: 442 4059 442
Size: 3617 738
BsaAI yAC'GTr
Cuts at: 1708 1708
Size: 4355
BsaBI GATnn'nnATC
Cuts at: 6 1269 1869 3906 6
Size: 1263 600 2037 455
Fragments arranged by size:
2037 1263 600 455
BsaHI Gr'CG_yC
Cuts at: 1786 2034 2736 4232 1786
Size: 248 702 1496 1909
Fragments arranged by size:
1909 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1544 2065 2743 3154 3301 3575 1544
Size: 521 678 411 147 274 2324
Fragments arranged by size:
2324 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 308 1340 1780 2538 3203 3611 308
Size: 1032 440 758 665 408 1052
Fragments arranged by size:
1052 1032 758 665 440 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 526 1268 526
Size: 742 3613
BsiEI CG_ry'CG
Cuts at: 104 1897 1943 3173 104
Size: 1793 46 1230 1286
Fragments arranged by size:
1793 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1369 2147 2337 3197 1369
Size: 778 190 860 2527
Fragments arranged by size:
2527 860 778 190
Bsp1286I G_dGCh'C
Cuts at: 152 1369 1980 2073 2147 2337 2399 3197 152
Size: 1217 611 93 74 190 62 798 1310
Fragments arranged by size:
1310 1217 798 611 190 93 74 62
BspHI T'CATG_A
Cuts at: 2787 2787
Size: 4355
BspMI ACCTGCnnnn'nnnn_
Cuts at: 498 1405 1619 1921 2302 498
Size: 907 214 302 381 2551
Fragments arranged by size:
2551 907 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2647 2701 4185 4350 50
Size: 2597 54 1484 165 55
Fragments arranged by size:
2597 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2267 2267
Size: 4355
BsrFI r'CCGG_y
Cuts at: 2353 2534 2353
Size: 181 4174
BsrGI T'GTAC_A
Cuts at: 700 700
Size: 4355
BssSI C'ACGA_G
Cuts at: 2626 3334 2626
Size: 708 3647
BstAPI GCAn_nnn'nTGC
Cuts at: 1276 1732 1276
Size: 456 3899
BstBI TT'CG_AA
Cuts at: 2716 2716
Size: 4355
BstEII G'GTnAC_C
Cuts at: 126 126
Size: 4355
BstYI r'GATC_y
Cuts at: 1 519 1380 1847 1870 2205 2451 2757 2769
Size: 518 861 467 23 335 246 306 12
Cuts at: 2769 2855 3907 1
Size: 86 1052 449
Fragments arranged by size:
1052 861 518 467 449 335 306 246
86 23 12
BtgI C'CryG_G
Cuts at: 194 442 194
Size: 248 4107
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2305 2486 2649 2305
Size: 181 163 4011
Fragments arranged by size:
4011 181 163
BtsI GCAGTG_nn'
Cuts at: 392 1296 1598 392
Size: 904 302 3149
Fragments arranged by size:
3149 904 302
DraI TTT'AAA
Cuts at: 1354 1354
Size: 4355
DrdI GACnn_nn'nnGTC
Cuts at: 2061 3405 2061
Size: 1344 3011
EaeI y'GGCC_r
Cuts at: 1940 2114 2505 2532 3877 1940
Size: 174 391 27 1345 2418
Fragments arranged by size:
2418 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1940 1940
Size: 4355
EarI CTCTTCn'nnn_
Cuts at: 2378 2588 2378
Size: 210 4145
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2563 3305 3451 4165 2563
Size: 742 146 714 2753
Fragments arranged by size:
2753 742 714 146
EcoICRI GAG'CTC
Cuts at: 1367 1367
Size: 4355
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 2180 2612 2965 3627 2180
Size: 432 353 662 2908
Fragments arranged by size:
2908 662 432 353
EcoO109I rG'GnC_Cy
Cuts at: 462 788 1494 462
Size: 326 706 3323
Fragments arranged by size:
3323 706 326
EcoRI G'AATT_C
Cuts at: 284 1359 284
Size: 1075 3280
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2374 2374
Size: 4355
FspI TGC'GCA
Cuts at: 827 2136 827
Size: 1309 3046
HaeII r_GCGC'y
Cuts at: 699 1789 2037 3267 4038 699
Size: 1090 248 1230 771 1016
Fragments arranged by size:
1230 1090 1016 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 364 396 1315 1347 2197 2227 2229 2259 364
Size: 32 919 32 850 30 2 30 2460
Fragments arranged by size:
2460 919 850 32 32 30 30 2
HincII GTy'rAC
Cuts at: 333 1394 333
Size: 1061 3294
KasI G'GCGC_C
Cuts at: 1785 2033 1785
Size: 248 4107
KpnI G_GTAC'C
Cuts at: 1375 1375
Size: 4355
MfeI C'AATT_G
Cuts at: 1253 1253
Size: 4355
MluI A'CGCG_T
Cuts at: 3788 3788
Size: 4355
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3114 3298 3114
Size: 184 4171
MscI TGG'CCA
Cuts at: 2116 2116
Size: 4355
MslI CAynn'nnrTG
Cuts at: 2344 2471 2344
Size: 127 4228
MspA1I CmG'CkG
Cuts at: 1466 1780 2140 2924 3169 1466
Size: 314 360 784 245 2652
Fragments arranged by size:
2652 784 360 314 245
NaeI GCC'GGC
Cuts at: 2536 2536
Size: 4355
NarI GG'CG_CC
Cuts at: 1786 2034 1786
Size: 248 4107
NcoI C'CATG_G
Cuts at: 442 442
Size: 4355
NdeI CA'TA_TG
Cuts at: 605 605
Size: 4355
NgoMIV G'CCGG_C
Cuts at: 2534 2534
Size: 4355
NspI r_CATG'y
Cuts at: 295 1125 1185 1408 1908 3511 295
Size: 830 60 223 500 1603 1139
Fragments arranged by size:
1603 1139 830 500 223 60
PciI A'CATG_T
Cuts at: 3507 3507
Size: 4355
PflMI CCAn_nnn'nTGG
Cuts at: 304 304
Size: 4355
PmeI GTTT'AAAC
Cuts at: 1354 1354
Size: 4355
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2801 2833 2801
Size: 32 4323
PpuMI rG'GwC_Cy
Cuts at: 462 462
Size: 4355
PstI C_TGCA'G
Cuts at: 1402 2087 1402
Size: 685 3670
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4355
PvuII CAG'CTG
Cuts at: 1780 2140 1780
Size: 360 3995
RsrII CG'GwC_CG
Cuts at: 2550 2550
Size: 4355
SacI G_AGCT'C
Cuts at: 1369 1369
Size: 4355
SalI G'TCGA_C
Cuts at: 1392 1392
Size: 4355
SapI GCTCTTCn'nnn_
Cuts at: 2378 2588 2378
Size: 210 4145
SbfI CC_TGCA'GG
Cuts at: 1402 1402
Size: 4355
ScaI AGT'ACT
Cuts at: 3872 3872
Size: 4355
SfcI C'TryA_G
Cuts at: 33 234 1096 1398 2083 3051 3242 33
Size: 201 862 302 685 968 191 1146
Fragments arranged by size:
1146 968 862 685 302 201 191
SfoI GGC'GCC
Cuts at: 1787 2035 1787
Size: 248 4107
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4355
SmaI CCC'GGG
Cuts at: 1377 1377
Size: 4355
SmlI C'TyrA_G
Cuts at: 523 1314 1510 2425 3139 3401 523
Size: 791 196 915 714 262 1477
Fragments arranged by size:
1477 915 791 714 262 196
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4355
SphI G_CATG'C
Cuts at: 295 1408 1908 295
Size: 1113 500 2742
Fragments arranged by size:
2742 1113 500
StuI AGG'CCT
Cuts at: 1204 1204
Size: 4355
StyI C'CwwG_G
Cuts at: 442 1173 1489 1843 442
Size: 731 316 354 2954
Fragments arranged by size:
2954 731 354 316
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1018 1938 2071 2530 2739 1018
Size: 920 133 459 209 2634
Fragments arranged by size:
2634 920 459 209 133
TatI w'GTAC_w
Cuts at: 700 3870 700
Size: 3170 1185
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 305 337 951 983 3872 3904 305
Size: 32 614 32 2889 32 756
Fragments arranged by size:
2889 756 614 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2152 2152
Size: 4355
XbaI T'CTAG_A
Cuts at: 1386 1386
Size: 4355
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 305 1850 305
Size: 1545 2810
XmaI C'CCGG_G
Cuts at: 1375 1375
Size: 4355
XmnI GAAnn'nnTTC
Cuts at: 119 284 119
Size: 165 4190
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AloI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NspI PciI PflMI PmeI PpiI PpuMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AgeI AhdI AleI AlfI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI
BpmI BsaXI BseRI BsiWI BsmI BsmBI BspEI BssHII
BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI NheI NotI NruI NsiI
PacI PasI PfoI PmlI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI SwaI XhoI ZraI