Restriction map for
pF1KB3509
(Circular) (Six-base) MAPSORT of: pF1KB3509.seq Check: 379 from: 1 to: 4211
pF1KB3509 4211 bp
With 182 enzymes: SgfI *
October 7, 2009 14:15 ..
AccI GT'mk_AC
Cuts at: 1249 1249
Size: 4211
Acc65I G'GTAC_C
Cuts at: 1227 1227
Size: 4211
AclI AA'CG_TT
Cuts at: 4051 4051
Size: 4211
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 335 2036 2468 3215 3483 335
Size: 1701 432 747 268 1063
Fragments arranged by size:
1701 1063 747 432 268
AflIII A'CryG_T
Cuts at: 1563 2667 3644 1563
Size: 1104 977 2130
Fragments arranged by size:
2130 1104 977
AgeI A'CCGG_T
Cuts at: 2599 2599
Size: 4211
AhdI GACnn_n'nnGTC
Cuts at: 487 487
Size: 4211
AleI CACnn'nnGTG
Cuts at: 697 697
Size: 4211
AlwNI CAG_nnn'CTG
Cuts at: 1516 3083 1516
Size: 1567 2644
ApaI G_GGCC'C
Cuts at: 119 119
Size: 4211
ApaLI G'TGCA_C
Cuts at: 700 2981 700
Size: 2281 1930
ApoI r'AATT_y
Cuts at: 242 438 784 864 1215 3564 3820 242
Size: 196 346 80 351 2349 256 633
Fragments arranged by size:
2349 633 351 346 256 196 80
AseI AT'TA_AT
Cuts at: 20 20
Size: 4211
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4211
AvaI C'yCGr_G
Cuts at: 1231 1231
Size: 4211
BamHI G'GATC_C
Cuts at: 1236 1236
Size: 4211
BanI G'GyrC_C
Cuts at: 1227 1641 1889 1924 1227
Size: 414 248 35 3514
Fragments arranged by size:
3514 414 248 35
BanII G_rGCy'C
Cuts at: 119 1225 2255 119
Size: 1106 1030 2075
Fragments arranged by size:
2075 1106 1030
BbeI G_GCGC'C
Cuts at: 1645 1893 1645
Size: 248 3963
BbsI GAAGACnn'nnnn_
Cuts at: 412 412
Size: 4211
BciVI GTATCCnnnnn_n'
Cuts at: 2109 2870 4211 2109
Size: 761 1341 2109
Fragments arranged by size:
2109 1341 761
BclI T'GATC_A
Cuts at: 1731 1731
Size: 4211
BglII A'GATC_T
Cuts at: 1726 3763 1726
Size: 2037 2174
BlpI GC'TnA_GC
Cuts at: 1323 1323
Size: 4211
Bme1580I G_kGCm'C
Cuts at: 119 704 1836 1929 2985 119
Size: 585 1132 93 1056 1345
Fragments arranged by size:
1345 1132 1056 585 93
BmrI ACTGGGnnnn_n'
Cuts at: 1423 1504 1839 3983 1423
Size: 81 335 2144 1651
Fragments arranged by size:
2144 1651 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2578 2578
Size: 4211
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 350 971 350
Size: 621 3590
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1387 2266 2758 3056 1387
Size: 879 492 298 2542
Fragments arranged by size:
2542 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 141 919 3915 141
Size: 778 2996 437
Fragments arranged by size:
2996 778 437
BsaAI yAC'GTr
Cuts at: 1564 1564
Size: 4211
BsaBI GATnn'nnATC
Cuts at: 6 1725 3762 6
Size: 1719 2037 455
Fragments arranged by size:
2037 1719 455
BsaHI Gr'CG_yC
Cuts at: 1642 1890 2592 4088 1642
Size: 248 702 1496 1765
Fragments arranged by size:
1765 1496 702 248
BsaWI w'CCGG_w
Cuts at: 680 1400 1921 2599 2873 3020 3431 680
Size: 720 521 678 274 147 411 1460
Fragments arranged by size:
1460 720 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 313 313
Size: 4211
BseYI C'CCAG_C
Cuts at: 112 147 1636 2394 2971 3467 112
Size: 35 1489 758 577 496 856
Fragments arranged by size:
1489 856 758 577 496 35
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 622 917 622
Size: 295 3916
BsiEI CG_ry'CG
Cuts at: 104 1753 1799 3007 104
Size: 1649 46 1208 1308
Fragments arranged by size:
1649 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 704 1225 2003 2193 2985 704
Size: 521 778 190 792 1930
Fragments arranged by size:
1930 792 778 521 190
BsmI GAATG_Cn'
Cuts at: 154 154
Size: 4211
Bsp1286I G_dGCh'C
Cuts at: 119 704 1225 1836 1929 2003 2193 2255 2985
Size: 585 521 611 93 74 190 62 730
Cuts at: 2985 119
Size: 1345
Fragments arranged by size:
1345 730 611 585 521 190 93 74
62
BspEI T'CCGG_A
Cuts at: 3431 3431
Size: 4211
BspHI T'CATG_A
Cuts at: 3387 3387
Size: 4211
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1261 1475 1777 2158 1261
Size: 214 302 381 3314
Fragments arranged by size:
3314 381 302 214
BsrBI CCG'CTC
Cuts at: 50 303 2503 2557 4041 4206 50
Size: 253 2200 54 1484 165 55
Fragments arranged by size:
2200 1484 253 165 55 54
BsrDI GCAATG_nn'
Cuts at: 641 992 1155 2123 641
Size: 351 163 968 2729
Fragments arranged by size:
2729 968 351 163
BsrFI r'CCGG_y
Cuts at: 421 2209 2390 2599 421
Size: 1788 181 209 2033
Fragments arranged by size:
2033 1788 209 181
BssSI C'ACGA_G
Cuts at: 2482 2840 2482
Size: 358 3853
BstAPI GCAn_nnn'nTGC
Cuts at: 1588 1588
Size: 4211
BstBI TT'CG_AA
Cuts at: 2572 2572
Size: 4211
BstEII G'GTnAC_C
Cuts at: 174 424 174
Size: 250 3961
BstYI r'GATC_y
Cuts at: 1 125 831 1236 1703 1726 2061 2307 3319
Size: 124 706 405 467 23 335 246 1012
Cuts at: 3319 3405 3417 3763 1
Size: 86 12 346 449
Fragments arranged by size:
1012 706 467 449 405 346 335 246
124 86 23 12
BtgI C'CryG_G
Cuts at: 107 368 107
Size: 261 3950
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2161 2342 2505 2161
Size: 181 163 3867
Fragments arranged by size:
3867 181 163
BtsI GCAGTG_nn'
Cuts at: 311 1454 311
Size: 1143 3068
DraI TTT'AAA
Cuts at: 1210 1210
Size: 4211
DraIII CAC_nnn'GTG
Cuts at: 931 931
Size: 4211
DrdI GACnn_nn'nnGTC
Cuts at: 1917 2775 1917
Size: 858 3353
EaeI y'GGCC_r
Cuts at: 1796 1970 2361 2388 3733 1796
Size: 174 391 27 1345 2274
Fragments arranged by size:
2274 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1796 1796
Size: 4211
EarI CTCTTCn'nnn_
Cuts at: 2234 2444 2234
Size: 210 4001
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2419 2729 2875 4021 2419
Size: 310 146 1146 2609
Fragments arranged by size:
2609 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1223 1223
Size: 4211
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 335 350 971 2036 2468 3215 3483 335
Size: 15 621 1065 432 747 268 1063
Fragments arranged by size:
1065 1063 747 621 432 268 15
EcoO109I rG'GnC_Cy
Cuts at: 325 397 1350 325
Size: 72 953 3186
Fragments arranged by size:
3186 953 72
EcoRI G'AATT_C
Cuts at: 1215 1215
Size: 4211
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 405 2230 405
Size: 1825 2386
FspI TGC'GCA
Cuts at: 451 1992 451
Size: 1541 2670
HaeII r_GCGC'y
Cuts at: 1645 1893 2915 3894 1645
Size: 248 1022 979 1962
Fragments arranged by size:
1962 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 473 505 897 929 2053 2083 2085 2115 473
Size: 32 392 32 1124 30 2 30 2569
Fragments arranged by size:
2569 1124 392 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1250 1250
Size: 4211
KasI G'GCGC_C
Cuts at: 1641 1889 1641
Size: 248 3963
KpnI G_GTAC'C
Cuts at: 1231 1231
Size: 4211
MluI A'CGCG_T
Cuts at: 3644 3644
Size: 4211
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 504 2882 3066 504
Size: 2378 184 1649
Fragments arranged by size:
2378 1649 184
MscI TGG'CCA
Cuts at: 1972 1972
Size: 4211
MslI CAynn'nnrTG
Cuts at: 697 721 2200 2327 697
Size: 24 1479 127 2581
Fragments arranged by size:
2581 1479 127 24
MspA1I CmG'CkG
Cuts at: 250 1322 1636 1996 3009 3254 250
Size: 1072 314 360 1013 245 1207
Fragments arranged by size:
1207 1072 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2392 2392
Size: 4211
NarI GG'CG_CC
Cuts at: 1642 1890 1642
Size: 248 3963
NcoI C'CATG_G
Cuts at: 107 368 107
Size: 261 3950
NgoMIV G'CCGG_C
Cuts at: 2390 2390
Size: 4211
NspI r_CATG'y
Cuts at: 583 1264 1764 2671 583
Size: 681 500 907 2123
Fragments arranged by size:
2123 907 681 500
PciI A'CATG_T
Cuts at: 2667 2667
Size: 4211
PflMI CCAn_nnn'nTGG
Cuts at: 226 226
Size: 4211
PfoI T'CCnGG_A
Cuts at: 219 219
Size: 4211
PmeI GTTT'AAAC
Cuts at: 1210 1210
Size: 4211
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3350 3382 3350
Size: 32 4179
PpuMI rG'GwC_Cy
Cuts at: 325 325
Size: 4211
PspOMI G'GGCC_C
Cuts at: 115 115
Size: 4211
PstI C_TGCA'G
Cuts at: 255 1258 1943 255
Size: 1003 685 2523
Fragments arranged by size:
2523 1003 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4211
PvuII CAG'CTG
Cuts at: 250 1636 1996 250
Size: 1386 360 2465
Fragments arranged by size:
2465 1386 360
RsrII CG'GwC_CG
Cuts at: 2406 2406
Size: 4211
SacI G_AGCT'C
Cuts at: 1225 1225
Size: 4211
SalI G'TCGA_C
Cuts at: 1248 1248
Size: 4211
SapI GCTCTTCn'nnn_
Cuts at: 2234 2444 2234
Size: 210 4001
SbfI CC_TGCA'GG
Cuts at: 1258 1258
Size: 4211
ScaI AGT'ACT
Cuts at: 3728 3728
Size: 4211
SfcI C'TryA_G
Cuts at: 33 251 1254 1939 2932 3123 33
Size: 218 1003 685 993 191 1121
Fragments arranged by size:
1121 1003 993 685 218 191
SfoI GGC'GCC
Cuts at: 1643 1891 1643
Size: 248 3963
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4211
SmaI CCC'GGG
Cuts at: 1233 1233
Size: 4211
SmlI C'TyrA_G
Cuts at: 1366 2281 2773 3035 1366
Size: 915 492 262 2542
Fragments arranged by size:
2542 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4211
SphI G_CATG'C
Cuts at: 1264 1764 1264
Size: 500 3711
SspI AAT'ATT
Cuts at: 531 531
Size: 4211
StyI C'CwwG_G
Cuts at: 107 368 1345 1699 107
Size: 261 977 354 2619
Fragments arranged by size:
2619 977 354 261
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 443 1794 1927 2386 2595 443
Size: 1351 133 459 209 2059
Fragments arranged by size:
2059 1351 459 209 133
TatI w'GTAC_w
Cuts at: 3726 3726
Size: 4211
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3728 3760 3728
Size: 32 4179
Tth111I GACn'n_nGTC
Cuts at: 2008 2008
Size: 4211
XbaI T'CTAG_A
Cuts at: 1242 1242
Size: 4211
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1706 1706
Size: 4211
XmaI C'CCGG_G
Cuts at: 1231 1231
Size: 4211
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PflMI PfoI PmeI PpiI
PpuMI PspOMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AlfI AloI AscI AvrII
BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI Bpu10I
BsaXI BsiWI BsmBI BsrGI BssHII BstXI BstZ17I Bsu36I
ClaI CspCI EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PmlI PshAI PsiI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI StuI SwaI XhoI XmnI
ZraI