Restriction map for
pF1KB3506
(Circular) (Six-base) MAPSORT of: pF1KB3506.seq Check: 4301 from: 1 to: 3920
pF1KB3506 3920 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AccI GT'mk_AC
Cuts at: 958 958
Size: 3920
Acc65I G'GTAC_C
Cuts at: 936 936
Size: 3920
AclI AA'CG_TT
Cuts at: 3760 3760
Size: 3920
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 708 1745 2177 2924 3192 708
Size: 1037 432 747 268 1436
Fragments arranged by size:
1436 1037 747 432 268
AflIII A'CryG_T
Cuts at: 1272 2376 3353 1272
Size: 1104 977 1839
Fragments arranged by size:
1839 1104 977
AgeI A'CCGG_T
Cuts at: 2308 2308
Size: 3920
AlwNI CAG_nnn'CTG
Cuts at: 522 1225 2792 522
Size: 703 1567 1650
Fragments arranged by size:
1650 1567 703
ApaLI G'TGCA_C
Cuts at: 2690 2690
Size: 3920
ApoI r'AATT_y
Cuts at: 924 3273 3529 924
Size: 2349 256 1315
Fragments arranged by size:
2349 1315 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3920
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3920
AvaI C'yCGr_G
Cuts at: 510 940 510
Size: 430 3490
BamHI G'GATC_C
Cuts at: 945 945
Size: 3920
BanI G'GyrC_C
Cuts at: 322 587 936 1350 1598 1633 322
Size: 265 349 414 248 35 2609
Fragments arranged by size:
2609 414 349 265 248 35
BanII G_rGCy'C
Cuts at: 771 934 1964 771
Size: 163 1030 2727
Fragments arranged by size:
2727 1030 163
BbeI G_GCGC'C
Cuts at: 326 591 1354 1602 326
Size: 265 763 248 2644
Fragments arranged by size:
2644 763 265 248
BbsI GAAGACnn'nnnn_
Cuts at: 174 174
Size: 3920
BciVI GTATCCnnnnn_n'
Cuts at: 1818 2579 3920 1818
Size: 761 1341 1818
Fragments arranged by size:
1818 1341 761
BclI T'GATC_A
Cuts at: 710 1440 710
Size: 730 3190
BglI GCCn_nnn'nGGC
Cuts at: 295 295
Size: 3920
BglII A'GATC_T
Cuts at: 1435 3472 1435
Size: 2037 1883
BlpI GC'TnA_GC
Cuts at: 280 361 606 1032 280
Size: 81 245 426 3168
Fragments arranged by size:
3168 426 245 81
Bme1580I G_kGCm'C
Cuts at: 390 1545 1638 2694 390
Size: 1155 93 1056 1616
Fragments arranged by size:
1616 1155 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 358 402 476 1132 1213 1548 3692 358
Size: 44 74 656 81 335 2144 586
Fragments arranged by size:
2144 656 586 335 81 74 44
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2287 2287
Size: 3920
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 159 532 876 159
Size: 373 344 3203
Fragments arranged by size:
3203 373 344
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1096 1975 2467 2765 1096
Size: 879 492 298 2251
Fragments arranged by size:
2251 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3624 3624
Size: 3920
BsaAI yAC'GTr
Cuts at: 1273 1273
Size: 3920
BsaBI GATnn'nnATC
Cuts at: 6 376 1434 3471 6
Size: 370 1058 2037 455
Fragments arranged by size:
2037 1058 455 370
BsaHI Gr'CG_yC
Cuts at: 323 588 1351 1599 2301 3797 323
Size: 265 763 248 702 1496 446
Fragments arranged by size:
1496 763 702 446 265 248
BsaWI w'CCGG_w
Cuts at: 581 1109 1630 2308 2582 2729 3140 581
Size: 528 521 678 274 147 411 1361
Fragments arranged by size:
1361 678 528 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 576 606 874 904 576
Size: 30 268 30 3592
Fragments arranged by size:
3592 268 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 447 874 447
Size: 427 3493
BseYI C'CCAG_C
Cuts at: 1345 2103 2680 3176 1345
Size: 758 577 496 2089
Fragments arranged by size:
2089 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 582 1462 1508 2716 104
Size: 478 880 46 1208 1308
Fragments arranged by size:
1308 1208 880 478 46
BsiHKAI G_wGCw'C
Cuts at: 934 1712 1902 2694 934
Size: 778 190 792 2160
Fragments arranged by size:
2160 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 390 771 934 1545 1638 1712 1902 1964 2694
Size: 381 163 611 93 74 190 62 730
Cuts at: 2694 390
Size: 1616
Fragments arranged by size:
1616 730 611 381 190 163 93 74
62
BspEI T'CCGG_A
Cuts at: 3140 3140
Size: 3920
BspHI T'CATG_A
Cuts at: 3096 3096
Size: 3920
BspMI ACCTGCnnnn'nnnn_
Cuts at: 970 1184 1486 1867 970
Size: 214 302 381 3023
Fragments arranged by size:
3023 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2212 2266 3750 3915 50
Size: 2162 54 1484 165 55
Fragments arranged by size:
2162 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 492 1832 492
Size: 1340 2580
BsrFI r'CCGG_y
Cuts at: 1918 2099 2308 1918
Size: 181 209 3530
Fragments arranged by size:
3530 209 181
BsrGI T'GTAC_A
Cuts at: 395 395
Size: 3920
BssSI C'ACGA_G
Cuts at: 2191 2549 2191
Size: 358 3562
BstAPI GCAn_nnn'nTGC
Cuts at: 1297 1297
Size: 3920
BstBI TT'CG_AA
Cuts at: 2281 2281
Size: 3920
BstYI r'GATC_y
Cuts at: 1 945 1412 1435 1770 2016 3028 3114 3126
Size: 944 467 23 335 246 1012 86 12
Cuts at: 3126 3472 1
Size: 346 449
Fragments arranged by size:
1012 944 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 704 107
Size: 597 3323
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 270 387 1870 2051 2214 270
Size: 117 1483 181 163 1976
Fragments arranged by size:
1976 1483 181 163 117
BtsI GCAGTG_nn'
Cuts at: 152 819 1163 152
Size: 667 344 2909
Fragments arranged by size:
2909 667 344
DraI TTT'AAA
Cuts at: 919 919
Size: 3920
DrdI GACnn_nn'nnGTC
Cuts at: 1626 2484 1626
Size: 858 3062
EaeI y'GGCC_r
Cuts at: 110 152 164 480 849 1505 1679 2070 2097
Size: 42 12 316 369 656 174 391 27
Cuts at: 2097 3442 110
Size: 1345 588
Fragments arranged by size:
1345 656 588 391 369 316 174 42
27 12
EagI C'GGCC_G
Cuts at: 1505 1505
Size: 3920
EarI CTCTTCn'nnn_
Cuts at: 139 1943 2153 139
Size: 1804 210 1906
Fragments arranged by size:
1906 1804 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2128 2438 2584 3730 2128
Size: 310 146 1146 2318
Fragments arranged by size:
2318 1146 310 146
EcoICRI GAG'CTC
Cuts at: 932 932
Size: 3920
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 159 532 708 876 1745 2177 2924 3192 159
Size: 373 176 168 869 432 747 268 887
Fragments arranged by size:
887 869 747 432 373 268 176 168
EcoO109I rG'GnC_Cy
Cuts at: 519 542 1059 519
Size: 23 517 3380
Fragments arranged by size:
3380 517 23
EcoRI G'AATT_C
Cuts at: 924 924
Size: 3920
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 150 1939 150
Size: 1789 2131
FspI TGC'GCA
Cuts at: 1701 1701
Size: 3920
HaeII r_GCGC'y
Cuts at: 326 591 1354 1602 2624 3603 326
Size: 265 763 248 1022 979 643
Fragments arranged by size:
1022 979 763 643 265 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 874 906 1762 1792 1794 1824 874
Size: 32 856 30 2 30 2970
Fragments arranged by size:
2970 856 32 30 30 2
HincII GTy'rAC
Cuts at: 959 959
Size: 3920
KasI G'GCGC_C
Cuts at: 322 587 1350 1598 322
Size: 265 763 248 2644
Fragments arranged by size:
2644 763 265 248
KpnI G_GTAC'C
Cuts at: 940 940
Size: 3920
MluI A'CGCG_T
Cuts at: 3353 3353
Size: 3920
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2591 2775 2591
Size: 184 3736
MscI TGG'CCA
Cuts at: 112 166 1681 112
Size: 54 1515 2351
Fragments arranged by size:
2351 1515 54
MslI CAynn'nnrTG
Cuts at: 1909 2036 1909
Size: 127 3793
MspA1I CmG'CkG
Cuts at: 320 365 552 706 848 1031 1345 1705 2718
Size: 45 187 154 142 183 314 360 1013
Cuts at: 2718 2963 320
Size: 245 1277
Fragments arranged by size:
1277 1013 360 314 245 187 183 154
142 45
NaeI GCC'GGC
Cuts at: 2101 2101
Size: 3920
NarI GG'CG_CC
Cuts at: 323 588 1351 1599 323
Size: 265 763 248 2644
Fragments arranged by size:
2644 763 265 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 3920
NgoMIV G'CCGG_C
Cuts at: 2099 2099
Size: 3920
NspI r_CATG'y
Cuts at: 973 1473 2380 973
Size: 500 907 2513
Fragments arranged by size:
2513 907 500
PasI CC'CwG_GG
Cuts at: 204 204
Size: 3920
PciI A'CATG_T
Cuts at: 2376 2376
Size: 3920
PflMI CCAn_nnn'nTGG
Cuts at: 414 522 414
Size: 108 3812
PfoI T'CCnGG_A
Cuts at: 245 245
Size: 3920
PmeI GTTT'AAAC
Cuts at: 919 919
Size: 3920
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3059 3091 3059
Size: 32 3888
PpuMI rG'GwC_Cy
Cuts at: 542 542
Size: 3920
PshAI GACnn'nnGTC
Cuts at: 241 241
Size: 3920
PstI C_TGCA'G
Cuts at: 967 1652 967
Size: 685 3235
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3920
PvuII CAG'CTG
Cuts at: 365 552 1345 1705 365
Size: 187 793 360 2580
Fragments arranged by size:
2580 793 360 187
RsrII CG'GwC_CG
Cuts at: 2115 2115
Size: 3920
SacI G_AGCT'C
Cuts at: 934 934
Size: 3920
SacII CC_GC'GG
Cuts at: 707 707
Size: 3920
SalI G'TCGA_C
Cuts at: 957 957
Size: 3920
SanDI GG'GwC_CC
Cuts at: 542 542
Size: 3920
SapI GCTCTTCn'nnn_
Cuts at: 139 1943 2153 139
Size: 1804 210 1906
Fragments arranged by size:
1906 1804 210
SbfI CC_TGCA'GG
Cuts at: 967 967
Size: 3920
ScaI AGT'ACT
Cuts at: 3437 3437
Size: 3920
SfcI C'TryA_G
Cuts at: 33 632 963 1648 2641 2832 33
Size: 599 331 685 993 191 1121
Fragments arranged by size:
1121 993 685 599 331 191
SfoI GGC'GCC
Cuts at: 324 589 1352 1600 324
Size: 265 763 248 2644
Fragments arranged by size:
2644 763 265 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3920
SmaI CCC'GGG
Cuts at: 942 942
Size: 3920
SmlI C'TyrA_G
Cuts at: 1075 1990 2482 2744 1075
Size: 915 492 262 2251
Fragments arranged by size:
2251 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3920
SphI G_CATG'C
Cuts at: 973 1473 973
Size: 500 3420
StyI C'CwwG_G
Cuts at: 107 597 639 1054 1408 107
Size: 490 42 415 354 2619
Fragments arranged by size:
2619 490 415 354 42
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 680 735 1503 1636 2095 2304 680
Size: 55 768 133 459 209 2296
Fragments arranged by size:
2296 768 459 209 133 55
TatI w'GTAC_w
Cuts at: 395 777 3435 395
Size: 382 2658 880
Fragments arranged by size:
2658 880 382
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 576 608 3437 3469 576
Size: 32 2829 32 1027
Fragments arranged by size:
2829 1027 32 32
Tth111I GACn'n_nGTC
Cuts at: 1717 1717
Size: 3920
XbaI T'CTAG_A
Cuts at: 951 951
Size: 3920
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 852 1415 852
Size: 563 3357
XmaI C'CCGG_G
Cuts at: 940 940
Size: 3920
XmnI GAAnn'nnTTC
Cuts at: 133 133
Size: 3920
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PstI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BmgBI
BmtI Bpu10I BsgI BsiWI BsmI BsmBI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PmlI PsiI PspOMI PspXI PsrI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI ZraI