Restriction map for
pF1KB3505
(Circular) (Six-base) MAPSORT of: pF1KB3505.seq Check: 9219 from: 1 to: 4175
pF1KB3505 4175 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AccI GT'mk_AC
Cuts at: 326 1213 326
Size: 887 3288
Acc65I G'GTAC_C
Cuts at: 1191 1191
Size: 4175
AclI AA'CG_TT
Cuts at: 4015 4015
Size: 4175
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 470 833 2000 2432 2785 3447 470
Size: 363 1167 432 353 662 1198
Fragments arranged by size:
1198 1167 662 432 363 353
AflIII A'CryG_T
Cuts at: 1078 1527 3327 3608 1078
Size: 449 1800 281 1645
Fragments arranged by size:
1800 1645 449 281
AlwNI CAG_nnn'CTG
Cuts at: 1480 2918 1480
Size: 1438 2737
ApaLI G'TGCA_C
Cuts at: 3013 3013
Size: 4175
ApoI r'AATT_y
Cuts at: 1179 3528 3784 1179
Size: 2349 256 1570
Fragments arranged by size:
2349 1570 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4175
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4175
AvaI C'yCGr_G
Cuts at: 404 536 1195 404
Size: 132 659 3384
Fragments arranged by size:
3384 659 132
BamHI G'GATC_C
Cuts at: 1200 1200
Size: 4175
BanI G'GyrC_C
Cuts at: 1191 1605 1853 1888 1191
Size: 414 248 35 3478
Fragments arranged by size:
3478 414 248 35
BanII G_rGCy'C
Cuts at: 952 1189 2219 952
Size: 237 1030 2908
Fragments arranged by size:
2908 1030 237
BbeI G_GCGC'C
Cuts at: 1609 1857 1609
Size: 248 3927
BbsI GAAGACnn'nnnn_
Cuts at: 1037 1037
Size: 4175
BciVI GTATCCnnnnn_n'
Cuts at: 2073 3129 4175 2073
Size: 1056 1046 2073
Fragments arranged by size:
2073 1056 1046
BclI T'GATC_A
Cuts at: 1695 1695
Size: 4175
BfrBI ATG'CAT
Cuts at: 837 837
Size: 4175
BglII A'GATC_T
Cuts at: 684 721 1690 3727 684
Size: 37 969 2037 1132
Fragments arranged by size:
2037 1132 969 37
BlpI GC'TnA_GC
Cuts at: 121 1287 121
Size: 1166 3009
Bme1580I G_kGCm'C
Cuts at: 1800 1893 3017 1800
Size: 93 1124 2958
Fragments arranged by size:
2958 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 1387 1468 1803 3947 1387
Size: 81 335 2144 1615
Fragments arranged by size:
2144 1615 335 81
BmtI G_CTAG'C
Cuts at: 439 439
Size: 4175
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2542 2542
Size: 4175
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 461 500 461
Size: 39 4136
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1351 2230 2944 3242 1351
Size: 879 714 298 2284
Fragments arranged by size:
2284 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 1168 3879 1168
Size: 2711 1464
BsaAI yAC'GTr
Cuts at: 1528 1528
Size: 4175
BsaBI GATnn'nnATC
Cuts at: 6 1689 3726 6
Size: 1683 2037 455
Fragments arranged by size:
2037 1683 455
BsaHI Gr'CG_yC
Cuts at: 1606 1854 2556 4052 1606
Size: 248 702 1496 1729
Fragments arranged by size:
1729 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1364 1885 2563 2974 3121 3395 1364
Size: 521 678 411 147 274 2144
Fragments arranged by size:
2144 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 313 313
Size: 4175
BseYI C'CCAG_C
Cuts at: 1600 2358 3023 3431 1600
Size: 758 665 408 2344
Fragments arranged by size:
2344 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 99 1123 99
Size: 1024 3151
BsiEI CG_ry'CG
Cuts at: 104 168 1717 1763 2993 104
Size: 64 1549 46 1230 1286
Fragments arranged by size:
1549 1286 1230 64 46
BsiHKAI G_wGCw'C
Cuts at: 1189 1967 2157 3017 1189
Size: 778 190 860 2347
Fragments arranged by size:
2347 860 778 190
BsmI GAATG_Cn'
Cuts at: 839 839
Size: 4175
Bsp1286I G_dGCh'C
Cuts at: 952 1189 1800 1893 1967 2157 2219 3017 952
Size: 237 611 93 74 190 62 798 2110
Fragments arranged by size:
2110 798 611 237 190 93 74 62
BspHI T'CATG_A
Cuts at: 2607 2607
Size: 4175
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1225 1439 1741 2122 1225
Size: 214 302 381 3278
Fragments arranged by size:
3278 381 302 214
BsrBI CCG'CTC
Cuts at: 50 150 729 2467 2521 4005 4170 50
Size: 100 579 1738 54 1484 165 55
Fragments arranged by size:
1738 1484 579 165 100 55 54
BsrDI GCAATG_nn'
Cuts at: 744 2087 744
Size: 1343 2832
BsrFI r'CCGG_y
Cuts at: 2173 2354 2173
Size: 181 3994
BsrGI T'GTAC_A
Cuts at: 513 513
Size: 4175
BssHII G'CGCG_C
Cuts at: 199 199
Size: 4175
BssSI C'ACGA_G
Cuts at: 2446 3154 2446
Size: 708 3467
BstAPI GCAn_nnn'nTGC
Cuts at: 475 1552 475
Size: 1077 3098
BstBI TT'CG_AA
Cuts at: 752 2536 752
Size: 1784 2391
BstXI CCAn_nnnn'nTGG
Cuts at: 283 283
Size: 4175
BstYI r'GATC_y
Cuts at: 1 532 684 721 921 1200 1667 1690 2025
Size: 531 152 37 200 279 467 23 335
Cuts at: 2025 2271 2577 2589 2675 3727 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
1052 531 467 449 335 306 279 246
200 152 86 37 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4175
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2125 2306 2469 2125
Size: 181 163 3831
Fragments arranged by size:
3831 181 163
BtsI GCAGTG_nn'
Cuts at: 326 1418 326
Size: 1092 3083
DraI TTT'AAA
Cuts at: 696 1174 696
Size: 478 3697
DraIII CAC_nnn'GTG
Cuts at: 790 790
Size: 4175
DrdI GACnn_nn'nnGTC
Cuts at: 1881 3225 1881
Size: 1344 2831
EaeI y'GGCC_r
Cuts at: 1760 1934 2325 2352 3697 1760
Size: 174 391 27 1345 2238
Fragments arranged by size:
2238 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1760 1760
Size: 4175
EarI CTCTTCn'nnn_
Cuts at: 290 992 2198 2408 290
Size: 702 1206 210 2057
Fragments arranged by size:
2057 1206 702 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 424 2383 3125 3271 3985 424
Size: 1959 742 146 714 614
Fragments arranged by size:
1959 742 714 614 146
EcoICRI GAG'CTC
Cuts at: 1187 1187
Size: 4175
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 461 470 500 833 2000 2432 2785 3447 461
Size: 9 30 333 1167 432 353 662 1189
Fragments arranged by size:
1189 1167 662 432 353 333 30 9
EcoNI CCTnn'n_nnAGG
Cuts at: 178 178
Size: 4175
EcoO109I rG'GnC_Cy
Cuts at: 1314 1314
Size: 4175
EcoRI G'AATT_C
Cuts at: 1179 1179
Size: 4175
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 405 942 2194 405
Size: 537 1252 2386
Fragments arranged by size:
2386 1252 537
FspI TGC'GCA
Cuts at: 1956 1956
Size: 4175
HaeII r_GCGC'y
Cuts at: 128 1609 1857 3087 3858 128
Size: 1481 248 1230 771 445
Fragments arranged by size:
1481 1230 771 445 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2017 2047 2049 2079 2017
Size: 30 2 30 4113
Fragments arranged by size:
4113 30 30 2
HincII GTy'rAC
Cuts at: 1214 1214
Size: 4175
KasI G'GCGC_C
Cuts at: 1605 1853 1605
Size: 248 3927
KpnI G_GTAC'C
Cuts at: 1195 1195
Size: 4175
MfeI C'AATT_G
Cuts at: 251 251
Size: 4175
MluI A'CGCG_T
Cuts at: 3608 3608
Size: 4175
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2934 3118 2934
Size: 184 3991
MscI TGG'CCA
Cuts at: 1936 1936
Size: 4175
MslI CAynn'nnrTG
Cuts at: 2164 2291 2164
Size: 127 4048
MspA1I CmG'CkG
Cuts at: 1006 1286 1600 1960 2744 2989 1006
Size: 280 314 360 784 245 2192
Fragments arranged by size:
2192 784 360 314 280 245
NaeI GCC'GGC
Cuts at: 2356 2356
Size: 4175
NarI GG'CG_CC
Cuts at: 1606 1854 1606
Size: 248 3927
NcoI C'CATG_G
Cuts at: 107 107
Size: 4175
NdeI CA'TA_TG
Cuts at: 865 993 865
Size: 128 4047
NgoMIV G'CCGG_C
Cuts at: 2354 2354
Size: 4175
NheI G'CTAG_C
Cuts at: 435 435
Size: 4175
NsiI A_TGCA'T
Cuts at: 839 839
Size: 4175
NspI r_CATG'y
Cuts at: 1082 1228 1728 3331 1082
Size: 146 500 1603 1926
Fragments arranged by size:
1926 1603 500 146
PciI A'CATG_T
Cuts at: 1078 3327 1078
Size: 2249 1926
PmeI GTTT'AAAC
Cuts at: 1174 1174
Size: 4175
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2621 2653 2621
Size: 32 4143
PsiI TTA'TAA
Cuts at: 487 487
Size: 4175
PstI C_TGCA'G
Cuts at: 471 564 1222 1907 471
Size: 93 658 685 2739
Fragments arranged by size:
2739 685 658 93
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4175
PvuII CAG'CTG
Cuts at: 1006 1600 1960 1006
Size: 594 360 3221
Fragments arranged by size:
3221 594 360
RsrII CG'GwC_CG
Cuts at: 2370 2370
Size: 4175
SacI G_AGCT'C
Cuts at: 1189 1189
Size: 4175
SalI G'TCGA_C
Cuts at: 1212 1212
Size: 4175
SapI GCTCTTCn'nnn_
Cuts at: 2198 2408 2198
Size: 210 3965
SbfI CC_TGCA'GG
Cuts at: 1222 1222
Size: 4175
ScaI AGT'ACT
Cuts at: 3692 3692
Size: 4175
SexAI A'CCwGG_T
Cuts at: 800 800
Size: 4175
SfcI C'TryA_G
Cuts at: 33 327 467 560 650 1218 1903 2871 3062
Size: 294 140 93 90 568 685 968 191
Cuts at: 3062 33
Size: 1146
Fragments arranged by size:
1146 968 685 568 294 191 140 93
90
SfoI GGC'GCC
Cuts at: 1607 1855 1607
Size: 248 3927
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4175
SmaI CCC'GGG
Cuts at: 406 1197 406
Size: 791 3384
SmlI C'TyrA_G
Cuts at: 1330 2245 2959 3221 1330
Size: 915 714 262 2284
Fragments arranged by size:
2284 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4175
SphI G_CATG'C
Cuts at: 1228 1728 1228
Size: 500 3675
SrfI GCCC'GGGC
Cuts at: 406 406
Size: 4175
StyI C'CwwG_G
Cuts at: 107 1309 1663 107
Size: 1202 354 2619
Fragments arranged by size:
2619 1202 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1084 1758 1891 2350 2559 1084
Size: 674 133 459 209 2700
Fragments arranged by size:
2700 674 459 209 133
TatI w'GTAC_w
Cuts at: 247 513 3690 247
Size: 266 3177 732
Fragments arranged by size:
3177 732 266
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 492 524 3692 3724 492
Size: 32 3168 32 943
Fragments arranged by size:
3168 943 32 32
Tth111I GACn'n_nGTC
Cuts at: 1972 1972
Size: 4175
XbaI T'CTAG_A
Cuts at: 1206 1206
Size: 4175
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1670 1670
Size: 4175
XmaI C'CCGG_G
Cuts at: 404 1195 404
Size: 791 3384
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BciVI BclI BfrBI BglII BlpI Bme1580I BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstXI BstYI BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NsiI NspI PciI
PmeI PpiI PsiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SrfI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AlfI
AloI ApaI AscI AvrII BaeI BbvCI BcgI BglI
BmgBI Bpu10I BsaXI BsiWI BsmBI BspEI BstEII BstZ17I
Bsu36I ClaI CspCI EcoRV FseI FspAI HindIII HpaI
NotI NruI PacI PasI PflMI PfoI PmlI PpuMI
PshAI PspOMI PspXI PsrI SacII SanDI SfiI SgrAI
SnaBI SspI StuI SwaI XhoI XmnI ZraI