Restriction map for
pF1KB3502
(Circular) (Six-base) MAPSORT of: pF1KB3502.seq Check: 6282 from: 1 to: 3842
pF1KB3502 3842 bp
With 182 enzymes: SgfI *
April 24, 2009 13:02 ..
AccI GT'mk_AC
Cuts at: 880 880
Size: 3842
Acc65I G'GTAC_C
Cuts at: 858 858
Size: 3842
AclI AA'CG_TT
Cuts at: 3682 3682
Size: 3842
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 389 1667 2099 2846 3114 389
Size: 1278 432 747 268 1117
Fragments arranged by size:
1278 1117 747 432 268
AflIII A'CryG_T
Cuts at: 1194 2298 3275 1194
Size: 1104 977 1761
Fragments arranged by size:
1761 1104 977
AgeI A'CCGG_T
Cuts at: 2230 2230
Size: 3842
AlwNI CAG_nnn'CTG
Cuts at: 129 1147 2714 129
Size: 1018 1567 1257
Fragments arranged by size:
1567 1257 1018
ApaLI G'TGCA_C
Cuts at: 2612 2612
Size: 3842
ApoI r'AATT_y
Cuts at: 157 846 3195 3451 157
Size: 689 2349 256 548
Fragments arranged by size:
2349 689 548 256
AseI AT'TA_AT
Cuts at: 20 308 20
Size: 288 3554
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3842
AvaI C'yCGr_G
Cuts at: 136 862 136
Size: 726 3116
BamHI G'GATC_C
Cuts at: 867 867
Size: 3842
BanI G'GyrC_C
Cuts at: 858 1272 1520 1555 858
Size: 414 248 35 3145
Fragments arranged by size:
3145 414 248 35
BanII G_rGCy'C
Cuts at: 155 856 1886 155
Size: 701 1030 2111
Fragments arranged by size:
2111 1030 701
BbeI G_GCGC'C
Cuts at: 1276 1524 1276
Size: 248 3594
BbsI GAAGACnn'nnnn_
Cuts at: 247 449 712 247
Size: 202 263 3377
Fragments arranged by size:
3377 263 202
BciVI GTATCCnnnnn_n'
Cuts at: 1740 2501 3842 1740
Size: 761 1341 1740
Fragments arranged by size:
1740 1341 761
BclI T'GATC_A
Cuts at: 1362 1362
Size: 3842
BglII A'GATC_T
Cuts at: 1357 3394 1357
Size: 2037 1805
BlpI GC'TnA_GC
Cuts at: 954 954
Size: 3842
Bme1580I G_kGCm'C
Cuts at: 1467 1560 2616 1467
Size: 93 1056 2693
Fragments arranged by size:
2693 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 758 1054 1135 1470 3614 758
Size: 296 81 335 2144 986
Fragments arranged by size:
2144 986 335 296 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2209 2209
Size: 3842
Bpu10I CC'TnA_GC
Cuts at: 130 766 130
Size: 636 3206
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1018 1897 2389 2687 1018
Size: 879 492 298 2173
Fragments arranged by size:
2173 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3546 3546
Size: 3842
BsaAI yAC'GTr
Cuts at: 256 1195 256
Size: 939 2903
BsaBI GATnn'nnATC
Cuts at: 6 341 1356 3393 6
Size: 335 1015 2037 455
Fragments arranged by size:
2037 1015 455 335
BsaHI Gr'CG_yC
Cuts at: 1273 1521 2223 3719 1273
Size: 248 702 1496 1396
Fragments arranged by size:
1496 1396 702 248
BsaWI w'CCGG_w
Cuts at: 438 1031 1552 2230 2504 2651 3062 438
Size: 593 521 678 274 147 411 1218
Fragments arranged by size:
1218 678 593 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 676 676
Size: 3842
BseYI C'CCAG_C
Cuts at: 1267 2025 2602 3098 1267
Size: 758 577 496 2011
Fragments arranged by size:
2011 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 733 733
Size: 3842
BsiEI CG_ry'CG
Cuts at: 104 1384 1430 2638 104
Size: 1280 46 1208 1308
Fragments arranged by size:
1308 1280 1208 46
BsiHKAI G_wGCw'C
Cuts at: 155 856 1634 1824 2616 155
Size: 701 778 190 792 1381
Fragments arranged by size:
1381 792 778 701 190
Bsp1286I G_dGCh'C
Cuts at: 155 856 1467 1560 1634 1824 1886 2616 155
Size: 701 611 93 74 190 62 730 1381
Fragments arranged by size:
1381 730 701 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3062 3062
Size: 3842
BspHI T'CATG_A
Cuts at: 3018 3018
Size: 3842
BspMI ACCTGCnnnn'nnnn_
Cuts at: 114 892 1106 1408 1789 114
Size: 778 214 302 381 2167
Fragments arranged by size:
2167 778 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2134 2188 3672 3837 50
Size: 2084 54 1484 165 55
Fragments arranged by size:
2084 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1754 1754
Size: 3842
BsrFI r'CCGG_y
Cuts at: 1840 2021 2230 1840
Size: 181 209 3452
Fragments arranged by size:
3452 209 181
BssSI C'ACGA_G
Cuts at: 148 2113 2471 148
Size: 1965 358 1519
Fragments arranged by size:
1965 1519 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1219 1219
Size: 3842
BstBI TT'CG_AA
Cuts at: 2203 2203
Size: 3842
BstYI r'GATC_y
Cuts at: 1 867 1334 1357 1692 1938 2950 3036 3048
Size: 866 467 23 335 246 1012 86 12
Cuts at: 3048 3394 1
Size: 346 449
Fragments arranged by size:
1012 866 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3842
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1792 1973 2136 1792
Size: 181 163 3498
Fragments arranged by size:
3498 181 163
BtsI GCAGTG_nn'
Cuts at: 1085 1085
Size: 3842
ClaI AT'CG_AT
Cuts at: 290 290
Size: 3842
DraI TTT'AAA
Cuts at: 841 841
Size: 3842
DrdI GACnn_nn'nnGTC
Cuts at: 1548 2406 1548
Size: 858 2984
EaeI y'GGCC_r
Cuts at: 1427 1601 1992 2019 3364 1427
Size: 174 391 27 1345 1905
Fragments arranged by size:
1905 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1427 1427
Size: 3842
EarI CTCTTCn'nnn_
Cuts at: 1865 2075 1865
Size: 210 3632
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2050 2360 2506 3652 2050
Size: 310 146 1146 2240
Fragments arranged by size:
2240 1146 310 146
EcoICRI GAG'CTC
Cuts at: 153 854 153
Size: 701 3141
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 389 1667 2099 2846 3114 389
Size: 1278 432 747 268 1117
Fragments arranged by size:
1278 1117 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 126 166 299 981 126
Size: 40 133 682 2987
Fragments arranged by size:
2987 682 133 40
EcoRI G'AATT_C
Cuts at: 846 846
Size: 3842
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 781 849 1861 781
Size: 68 1012 2762
Fragments arranged by size:
2762 1012 68
FspI TGC'GCA
Cuts at: 1623 1623
Size: 3842
HaeII r_GCGC'y
Cuts at: 1276 1524 2546 3525 1276
Size: 248 1022 979 1593
Fragments arranged by size:
1593 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1684 1714 1716 1746 1684
Size: 30 2 30 3780
Fragments arranged by size:
3780 30 30 2
HincII GTy'rAC
Cuts at: 881 881
Size: 3842
HindIII A'AGCT_T
Cuts at: 199 199
Size: 3842
KasI G'GCGC_C
Cuts at: 1272 1520 1272
Size: 248 3594
KpnI G_GTAC'C
Cuts at: 862 862
Size: 3842
MluI A'CGCG_T
Cuts at: 3275 3275
Size: 3842
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 418 2513 2697 418
Size: 2095 184 1563
Fragments arranged by size:
2095 1563 184
MscI TGG'CCA
Cuts at: 1603 1603
Size: 3842
MslI CAynn'nnrTG
Cuts at: 1831 1958 1831
Size: 127 3715
MspA1I CmG'CkG
Cuts at: 281 953 1267 1627 2640 2885 281
Size: 672 314 360 1013 245 1238
Fragments arranged by size:
1238 1013 672 360 314 245
NaeI GCC'GGC
Cuts at: 2023 2023
Size: 3842
NarI GG'CG_CC
Cuts at: 1273 1521 1273
Size: 248 3594
NcoI C'CATG_G
Cuts at: 107 107
Size: 3842
NgoMIV G'CCGG_C
Cuts at: 2021 2021
Size: 3842
NspI r_CATG'y
Cuts at: 895 1395 2302 895
Size: 500 907 2435
Fragments arranged by size:
2435 907 500
PciI A'CATG_T
Cuts at: 2298 2298
Size: 3842
PmeI GTTT'AAAC
Cuts at: 841 841
Size: 3842
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2981 3013 2981
Size: 32 3810
PpuMI rG'GwC_Cy
Cuts at: 126 166 126
Size: 40 3802
PspXI vC'TCGA_Gb
Cuts at: 136 136
Size: 3842
PstI C_TGCA'G
Cuts at: 889 1574 889
Size: 685 3157
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3842
PvuII CAG'CTG
Cuts at: 1267 1627 1267
Size: 360 3482
RsrII CG'GwC_CG
Cuts at: 2037 2037
Size: 3842
SacI G_AGCT'C
Cuts at: 155 856 155
Size: 701 3141
SalI G'TCGA_C
Cuts at: 879 879
Size: 3842
SapI GCTCTTCn'nnn_
Cuts at: 1865 2075 1865
Size: 210 3632
SbfI CC_TGCA'GG
Cuts at: 889 889
Size: 3842
ScaI AGT'ACT
Cuts at: 3359 3359
Size: 3842
SfcI C'TryA_G
Cuts at: 33 245 885 1570 2563 2754 33
Size: 212 640 685 993 191 1121
Fragments arranged by size:
1121 993 685 640 212 191
SfoI GGC'GCC
Cuts at: 1274 1522 1274
Size: 248 3594
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3842
SmaI CCC'GGG
Cuts at: 864 864
Size: 3842
SmlI C'TyrA_G
Cuts at: 136 997 1912 2404 2666 136
Size: 861 915 492 262 1312
Fragments arranged by size:
1312 915 861 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3842
SphI G_CATG'C
Cuts at: 895 1395 895
Size: 500 3342
StuI AGG'CCT
Cuts at: 764 764
Size: 3842
StyI C'CwwG_G
Cuts at: 107 833 976 1330 107
Size: 726 143 354 2619
Fragments arranged by size:
2619 726 354 143
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 233 1425 1558 2017 2226 233
Size: 1192 133 459 209 1849
Fragments arranged by size:
1849 1192 459 209 133
TatI w'GTAC_w
Cuts at: 3357 3357
Size: 3842
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3359 3391 3359
Size: 32 3810
Tth111I GACn'n_nGTC
Cuts at: 1639 1639
Size: 3842
XbaI T'CTAG_A
Cuts at: 873 873
Size: 3842
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 1337 114
Size: 1223 2619
XhoI C'TCGA_G
Cuts at: 136 136
Size: 3842
XmaI C'CCGG_G
Cuts at: 862 862
Size: 3842
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglII BlpI Bme1580I BmrI BplI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI ClaI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PmeI PpiI PpuMI PspXI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI
BmgBI BmtI BpmI BsaXI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I CspCI DraIII EcoNI
EcoRV FseI FspAI HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PfoI PmlI PshAI
PsiI PspOMI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI SwaI XmnI ZraI