Restriction map for
pF1KB3501
(Circular) (Six-base) MAPSORT of: pF1KB3501.seq Check: 735 from: 1 to: 4253
pF1KB3501 4253 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AccI GT'mk_AC
Cuts at: 1291 1291
Size: 4253
Acc65I G'GTAC_C
Cuts at: 1269 1269
Size: 4253
AclI AA'CG_TT
Cuts at: 4093 4093
Size: 4253
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 322 406 848 2078 2510 2863 3525 322
Size: 84 442 1230 432 353 662 1050
Fragments arranged by size:
1230 1050 662 442 432 353 84
AflIII A'CryG_T
Cuts at: 197 266 957 1605 3405 3686 197
Size: 69 691 648 1800 281 764
Fragments arranged by size:
1800 764 691 648 281 69
AlwNI CAG_nnn'CTG
Cuts at: 871 971 1194 1558 2996 871
Size: 100 223 364 1438 2128
Fragments arranged by size:
2128 1438 364 223 100
ApaLI G'TGCA_C
Cuts at: 3091 3091
Size: 4253
ApoI r'AATT_y
Cuts at: 336 379 416 444 1141 1257 3606 3862 336
Size: 43 37 28 697 116 2349 256 727
Fragments arranged by size:
2349 727 697 256 116 43 37 28
AseI AT'TA_AT
Cuts at: 20 20
Size: 4253
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4253
AvaI C'yCGr_G
Cuts at: 128 246 1273 128
Size: 118 1027 3108
Fragments arranged by size:
3108 1027 118
BamHI G'GATC_C
Cuts at: 614 839 1278 614
Size: 225 439 3589
Fragments arranged by size:
3589 439 225
BanI G'GyrC_C
Cuts at: 252 1269 1683 1931 1966 252
Size: 1017 414 248 35 2539
Fragments arranged by size:
2539 1017 414 248 35
BanII G_rGCy'C
Cuts at: 1220 1267 2297 1220
Size: 47 1030 3176
Fragments arranged by size:
3176 1030 47
BbeI G_GCGC'C
Cuts at: 256 1687 1935 256
Size: 1431 248 2574
Fragments arranged by size:
2574 1431 248
BciVI GTATCCnnnnn_n'
Cuts at: 2151 3207 4253 2151
Size: 1056 1046 2151
Fragments arranged by size:
2151 1056 1046
BclI T'GATC_A
Cuts at: 1773 1773
Size: 4253
BfrBI ATG'CAT
Cuts at: 1206 1206
Size: 4253
BglII A'GATC_T
Cuts at: 1104 1768 3805 1104
Size: 664 2037 1552
Fragments arranged by size:
2037 1552 664
BlpI GC'TnA_GC
Cuts at: 160 1003 1365 160
Size: 843 362 3048
Fragments arranged by size:
3048 843 362
Bme1580I G_kGCm'C
Cuts at: 329 1878 1971 3095 329
Size: 1549 93 1124 1487
Fragments arranged by size:
1549 1487 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 1465 1546 1881 4025 1465
Size: 81 335 2144 1693
Fragments arranged by size:
2144 1693 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2620 2620
Size: 4253
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1429 2308 3022 3320 1429
Size: 879 714 298 2362
Fragments arranged by size:
2362 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 3957 3957
Size: 4253
BsaAI yAC'GTr
Cuts at: 198 1606 198
Size: 1408 2845
BsaBI GATnn'nnATC
Cuts at: 6 1767 3804 6
Size: 1761 2037 455
Fragments arranged by size:
2037 1761 455
BsaHI Gr'CG_yC
Cuts at: 253 1684 1932 2634 4130 253
Size: 1431 248 702 1496 376
Fragments arranged by size:
1496 1431 702 376 248
BsaWI w'CCGG_w
Cuts at: 284 539 1442 1963 2641 3052 3199 3473 284
Size: 255 903 521 678 411 147 274 1064
Fragments arranged by size:
1064 903 678 521 411 274 255 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 618 648 618
Size: 30 4223
BseYI C'CCAG_C
Cuts at: 144 742 1678 2436 3101 3509 144
Size: 598 936 758 665 408 888
Fragments arranged by size:
936 888 758 665 598 408
BsiEI CG_ry'CG
Cuts at: 104 1795 1841 3071 104
Size: 1691 46 1230 1286
Fragments arranged by size:
1691 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1009 1267 2045 2235 3095 1009
Size: 258 778 190 860 2167
Fragments arranged by size:
2167 860 778 258 190
BsmI GAATG_Cn'
Cuts at: 1208 1208
Size: 4253
Bsp1286I G_dGCh'C
Cuts at: 329 1009 1220 1267 1878 1971 2045 2235 2297
Size: 680 211 47 611 93 74 190 62
Cuts at: 2297 3095 329
Size: 798 1487
Fragments arranged by size:
1487 798 680 611 211 190 93 74
62 47
BspHI T'CATG_A
Cuts at: 2685 2685
Size: 4253
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1303 1517 1819 2200 1303
Size: 214 302 381 3356
Fragments arranged by size:
3356 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2545 2599 4083 4248 50
Size: 2495 54 1484 165 55
Fragments arranged by size:
2495 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2165 2165
Size: 4253
BsrFI r'CCGG_y
Cuts at: 2251 2432 2251
Size: 181 4072
BsrGI T'GTAC_A
Cuts at: 263 1022 263
Size: 759 3494
BssSI C'ACGA_G
Cuts at: 2524 3232 2524
Size: 708 3545
BstAPI GCAn_nnn'nTGC
Cuts at: 1630 1630
Size: 4253
BstBI TT'CG_AA
Cuts at: 2614 2614
Size: 4253
BstXI CCAn_nnnn'nTGG
Cuts at: 144 750 1063 144
Size: 606 313 3334
Fragments arranged by size:
3334 606 313
BstYI r'GATC_y
Cuts at: 1 614 839 1052 1104 1278 1745 1768 2103
Size: 613 225 213 52 174 467 23 335
Cuts at: 2103 2349 2655 2667 2753 3805 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
1052 613 467 449 335 306 246 225
213 174 86 52 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2203 2384 2547 2203
Size: 181 163 3909
Fragments arranged by size:
3909 181 163
BtsI GCAGTG_nn'
Cuts at: 1496 1496
Size: 4253
DraI TTT'AAA
Cuts at: 812 1252 812
Size: 440 3813
DraIII CAC_nnn'GTG
Cuts at: 1154 1243 1154
Size: 89 4164
DrdI GACnn_nn'nnGTC
Cuts at: 1959 3303 1959
Size: 1344 2909
EaeI y'GGCC_r
Cuts at: 937 1072 1838 2012 2403 2430 3775 937
Size: 135 766 174 391 27 1345 1415
Fragments arranged by size:
1415 1345 766 391 174 135 27
EagI C'GGCC_G
Cuts at: 1838 1838
Size: 4253
EarI CTCTTCn'nnn_
Cuts at: 1043 2276 2486 1043
Size: 1233 210 2810
Fragments arranged by size:
2810 1233 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2461 3203 3349 4063 2461
Size: 742 146 714 2651
Fragments arranged by size:
2651 742 714 146
EcoICRI GAG'CTC
Cuts at: 1265 1265
Size: 4253
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 322 406 848 2078 2510 2863 3525 322
Size: 84 442 1230 432 353 662 1050
Fragments arranged by size:
1230 1050 662 442 432 353 84
EcoNI CCTnn'n_nnAGG
Cuts at: 208 208
Size: 4253
EcoO109I rG'GnC_Cy
Cuts at: 1392 1392
Size: 4253
EcoRI G'AATT_C
Cuts at: 336 1257 336
Size: 921 3332
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 436 2272 436
Size: 1836 2417
FspI TGC'GCA
Cuts at: 2034 2034
Size: 4253
HaeII r_GCGC'y
Cuts at: 256 1687 1935 3165 3936 256
Size: 1431 248 1230 771 573
Fragments arranged by size:
1431 1230 771 573 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2095 2125 2127 2157 2095
Size: 30 2 30 4191
Fragments arranged by size:
4191 30 30 2
HincII GTy'rAC
Cuts at: 891 963 1292 891
Size: 72 329 3852
Fragments arranged by size:
3852 329 72
HindIII A'AGCT_T
Cuts at: 817 817
Size: 4253
KasI G'GCGC_C
Cuts at: 252 1683 1931 252
Size: 1431 248 2574
Fragments arranged by size:
2574 1431 248
KpnI G_GTAC'C
Cuts at: 1273 1273
Size: 4253
MluI A'CGCG_T
Cuts at: 3686 3686
Size: 4253
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 582 690 951 3012 3196 582
Size: 108 261 2061 184 1639
Fragments arranged by size:
2061 1639 261 184 108
MscI TGG'CCA
Cuts at: 2014 2014
Size: 4253
MslI CAynn'nnrTG
Cuts at: 431 512 1061 1121 2242 2369 431
Size: 81 549 60 1121 127 2315
Fragments arranged by size:
2315 1121 549 127 81 60
MspA1I CmG'CkG
Cuts at: 1173 1191 1364 1678 2038 2822 3067 1173
Size: 18 173 314 360 784 245 2359
Fragments arranged by size:
2359 784 360 314 245 173 18
NaeI GCC'GGC
Cuts at: 2434 2434
Size: 4253
NarI GG'CG_CC
Cuts at: 253 1684 1932 253
Size: 1431 248 2574
Fragments arranged by size:
2574 1431 248
NgoMIV G'CCGG_C
Cuts at: 2432 2432
Size: 4253
NsiI A_TGCA'T
Cuts at: 1208 1208
Size: 4253
NspI r_CATG'y
Cuts at: 270 430 961 1306 1806 3409 270
Size: 160 531 345 500 1603 1114
Fragments arranged by size:
1603 1114 531 500 345 160
PciI A'CATG_T
Cuts at: 266 957 3405 266
Size: 691 2448 1114
Fragments arranged by size:
2448 1114 691
PflMI CCAn_nnn'nTGG
Cuts at: 836 1154 836
Size: 318 3935
PfoI T'CCnGG_A
Cuts at: 749 749
Size: 4253
PmeI GTTT'AAAC
Cuts at: 1252 1252
Size: 4253
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2699 2731 2699
Size: 32 4221
PsiI TTA'TAA
Cuts at: 584 584
Size: 4253
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1128 1160 1128
Size: 32 4221
PstI C_TGCA'G
Cuts at: 1300 1985 1300
Size: 685 3568
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4253
PvuII CAG'CTG
Cuts at: 1173 1191 1678 2038 1173
Size: 18 487 360 3388
Fragments arranged by size:
3388 487 360 18
RsrII CG'GwC_CG
Cuts at: 2448 2448
Size: 4253
SacI G_AGCT'C
Cuts at: 1267 1267
Size: 4253
SalI G'TCGA_C
Cuts at: 1290 1290
Size: 4253
SapI GCTCTTCn'nnn_
Cuts at: 2276 2486 2276
Size: 210 4043
SbfI CC_TGCA'GG
Cuts at: 1300 1300
Size: 4253
ScaI AGT'ACT
Cuts at: 3770 3770
Size: 4253
SfcI C'TryA_G
Cuts at: 33 1296 1981 2949 3140 33
Size: 1263 685 968 191 1146
Fragments arranged by size:
1263 1146 968 685 191
SfoI GGC'GCC
Cuts at: 254 1685 1933 254
Size: 1431 248 2574
Fragments arranged by size:
2574 1431 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4253
SmaI CCC'GGG
Cuts at: 130 248 1275 130
Size: 118 1027 3108
Fragments arranged by size:
3108 1027 118
SmlI C'TyrA_G
Cuts at: 1408 2323 3037 3299 1408
Size: 915 714 262 2362
Fragments arranged by size:
2362 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4253
SphI G_CATG'C
Cuts at: 1306 1806 1306
Size: 500 3753
SspI AAT'ATT
Cuts at: 588 760 588
Size: 172 4081
StyI C'CwwG_G
Cuts at: 1387 1741 1387
Size: 354 3899
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 361 579 1836 1969 2428 2637 361
Size: 218 1257 133 459 209 1977
Fragments arranged by size:
1977 1257 459 218 209 133
TatI w'GTAC_w
Cuts at: 263 1022 3768 263
Size: 759 2746 748
Fragments arranged by size:
2746 759 748
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 618 650 3770 3802 618
Size: 32 3120 32 1069
Fragments arranged by size:
3120 1069 32 32
Tth111I GACn'n_nGTC
Cuts at: 1233 2050 1233
Size: 817 3436
XbaI T'CTAG_A
Cuts at: 309 1284 309
Size: 975 3278
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 706 1748 706
Size: 1042 3211
XmaI C'CCGG_G
Cuts at: 128 246 1273 128
Size: 118 1027 3108
Fragments arranged by size:
3108 1027 118
XmnI GAAnn'nnTTC
Cuts at: 220 419 1106 220
Size: 199 687 3367
Fragments arranged by size:
3367 687 199
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BciVI
BclI BfrBI BglII BlpI Bme1580I BmrI BplI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsiEI
BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstXI BstYI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NsiI NspI PciI PflMI
PfoI PmeI PpiI PsiI PsrI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AlfI
AloI ApaI AscI AvrII BaeI BbsI BbvCI BcgI
BglI BmgBI BmtI BpmI Bpu10I BseRI BsgI BsiWI
BsmBI BspEI BssHII BstEII BstZ17I Bsu36I BtgI ClaI
CspCI EcoRV FseI FspAI HpaI MfeI NcoI NdeI
NheI NotI NruI PacI PasI PmlI PpuMI PshAI
PspOMI PspXI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI StuI SwaI XhoI ZraI