Restriction map for
pF1KB3492
(Circular) (Six-base) MAPSORT of: pF1KB3492.seq Check: 3705 from: 1 to: 4304
pF1KB3492 4304 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AccI GT'mk_AC
Cuts at: 543 1342 543
Size: 799 3505
Acc65I G'GTAC_C
Cuts at: 1320 1320
Size: 4304
AclI AA'CG_TT
Cuts at: 4144 4144
Size: 4304
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 845 2129 2561 2914 3576 845
Size: 1284 432 353 662 1573
Fragments arranged by size:
1573 1284 662 432 353
AflIII A'CryG_T
Cuts at: 282 1656 3456 3737 282
Size: 1374 1800 281 849
Fragments arranged by size:
1800 1374 849 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 384 1269 384
Size: 885 3419
AlwNI CAG_nnn'CTG
Cuts at: 127 204 913 1609 3047 127
Size: 77 709 696 1438 1384
Fragments arranged by size:
1438 1384 709 696 77
ApaLI G'TGCA_C
Cuts at: 3142 3142
Size: 4304
ApoI r'AATT_y
Cuts at: 341 1190 1308 3657 3913 341
Size: 849 118 2349 256 732
Fragments arranged by size:
2349 849 732 256 118
AseI AT'TA_AT
Cuts at: 20 20
Size: 4304
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4304
AvaI C'yCGr_G
Cuts at: 1324 1324
Size: 4304
BamHI G'GATC_C
Cuts at: 1329 1329
Size: 4304
BanI G'GyrC_C
Cuts at: 175 1320 1734 1982 2017 175
Size: 1145 414 248 35 2462
Fragments arranged by size:
2462 1145 414 248 35
BanII G_rGCy'C
Cuts at: 211 1318 2348 211
Size: 1107 1030 2167
Fragments arranged by size:
2167 1107 1030
BbeI G_GCGC'C
Cuts at: 1738 1986 1738
Size: 248 4056
BciVI GTATCCnnnnn_n'
Cuts at: 2202 3258 4304 2202
Size: 1056 1046 2202
Fragments arranged by size:
2202 1056 1046
BclI T'GATC_A
Cuts at: 1824 1824
Size: 4304
BfrBI ATG'CAT
Cuts at: 568 568
Size: 4304
BglI GCCn_nnn'nGGC
Cuts at: 247 247
Size: 4304
BglII A'GATC_T
Cuts at: 1819 3856 1819
Size: 2037 2267
BlpI GC'TnA_GC
Cuts at: 1416 1416
Size: 4304
Bme1580I G_kGCm'C
Cuts at: 1929 2022 3146 1929
Size: 93 1124 3087
Fragments arranged by size:
3087 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 1516 1597 1932 4076 1516
Size: 81 335 2144 1744
Fragments arranged by size:
2144 1744 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 497 2671 497
Size: 2174 2130
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 423 1237 423
Size: 814 3490
Bpu10I CC'TnA_GC
Cuts at: 180 180
Size: 4304
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 519 1480 2359 3073 3371 519
Size: 961 879 714 298 1452
Fragments arranged by size:
1452 961 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 4008 4008
Size: 4304
BsaAI yAC'GTr
Cuts at: 1657 1657
Size: 4304
BsaBI GATnn'nnATC
Cuts at: 6 1818 3855 6
Size: 1812 2037 455
Fragments arranged by size:
2037 1812 455
BsaHI Gr'CG_yC
Cuts at: 1735 1983 2685 4181 1735
Size: 248 702 1496 1858
Fragments arranged by size:
1858 1496 702 248
BsaWI w'CCGG_w
Cuts at: 252 1493 2014 2692 3103 3250 3524 252
Size: 1241 521 678 411 147 274 1032
Fragments arranged by size:
1241 1032 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 193 223 234 264 193
Size: 30 11 30 4233
Fragments arranged by size:
4233 30 30 11
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 473 473
Size: 4304
BseYI C'CCAG_C
Cuts at: 849 1729 2487 3152 3560 849
Size: 880 758 665 408 1593
Fragments arranged by size:
1593 880 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 356 356
Size: 4304
BsiEI CG_ry'CG
Cuts at: 104 1846 1892 3122 104
Size: 1742 46 1230 1286
Fragments arranged by size:
1742 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1318 2096 2286 3146 1318
Size: 778 190 860 2476
Fragments arranged by size:
2476 860 778 190
BsmI GAATG_Cn'
Cuts at: 117 684 117
Size: 567 3737
BsmBI CGTCTCn'nnnn_
Cuts at: 144 144
Size: 4304
Bsp1286I G_dGCh'C
Cuts at: 211 1318 1929 2022 2096 2286 2348 3146 211
Size: 1107 611 93 74 190 62 798 1369
Fragments arranged by size:
1369 1107 798 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 252 252
Size: 4304
BspHI T'CATG_A
Cuts at: 2736 2736
Size: 4304
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1021 1354 1568 1870 2251 1021
Size: 333 214 302 381 3074
Fragments arranged by size:
3074 381 333 302 214
BsrBI CCG'CTC
Cuts at: 50 2596 2650 4134 4299 50
Size: 2546 54 1484 165 55
Fragments arranged by size:
2546 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1286 2216 1286
Size: 930 3374
BsrFI r'CCGG_y
Cuts at: 2302 2483 2302
Size: 181 4123
BssSI C'ACGA_G
Cuts at: 2575 3283 2575
Size: 708 3596
BstAPI GCAn_nnn'nTGC
Cuts at: 1681 1681
Size: 4304
BstBI TT'CG_AA
Cuts at: 2665 2665
Size: 4304
BstXI CCAn_nnnn'nTGG
Cuts at: 447 1171 447
Size: 724 3580
BstYI r'GATC_y
Cuts at: 1 123 1329 1796 1819 2154 2400 2706 2718
Size: 122 1206 467 23 335 246 306 12
Cuts at: 2718 2804 3856 1
Size: 86 1052 449
Fragments arranged by size:
1206 1052 467 449 335 306 246 122
86 23 12
BstZ17I GTA'TAC
Cuts at: 544 544
Size: 4304
BtgI C'CryG_G
Cuts at: 777 777
Size: 4304
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2254 2435 2598 2254
Size: 181 163 3960
Fragments arranged by size:
3960 181 163
BtsI GCAGTG_nn'
Cuts at: 1547 1547
Size: 4304
DraI TTT'AAA
Cuts at: 1303 1303
Size: 4304
DrdI GACnn_nn'nnGTC
Cuts at: 2010 3354 2010
Size: 1344 2960
EaeI y'GGCC_r
Cuts at: 693 1889 2063 2454 2481 3826 693
Size: 1196 174 391 27 1345 1171
Fragments arranged by size:
1345 1196 1171 391 174 27
EagI C'GGCC_G
Cuts at: 1889 1889
Size: 4304
EarI CTCTTCn'nnn_
Cuts at: 229 732 2327 2537 229
Size: 503 1595 210 1996
Fragments arranged by size:
1996 1595 503 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2512 3254 3400 4114 2512
Size: 742 146 714 2702
Fragments arranged by size:
2702 742 714 146
EcoICRI GAG'CTC
Cuts at: 1316 1316
Size: 4304
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 423 845 1237 2129 2561 2914 3576 423
Size: 422 392 892 432 353 662 1151
Fragments arranged by size:
1151 892 662 432 422 392 353
EcoO109I rG'GnC_Cy
Cuts at: 201 1443 201
Size: 1242 3062
EcoRI G'AATT_C
Cuts at: 341 1190 1308 341
Size: 849 118 3337
Fragments arranged by size:
3337 849 118
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 834 2323 834
Size: 1489 2815
FspI TGC'GCA
Cuts at: 2085 2085
Size: 4304
HaeII r_GCGC'y
Cuts at: 1738 1986 3216 3987 1738
Size: 248 1230 771 2055
Fragments arranged by size:
2055 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 193 225 2146 2176 2178 2208 193
Size: 32 1921 30 2 30 2289
Fragments arranged by size:
2289 1921 32 30 30 2
HincII GTy'rAC
Cuts at: 731 1053 1343 731
Size: 322 290 3692
Fragments arranged by size:
3692 322 290
KasI G'GCGC_C
Cuts at: 1734 1982 1734
Size: 248 4056
KpnI G_GTAC'C
Cuts at: 1324 1324
Size: 4304
MluI A'CGCG_T
Cuts at: 3737 3737
Size: 4304
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3063 3247 3063
Size: 184 4120
MscI TGG'CCA
Cuts at: 695 2065 695
Size: 1370 2934
MslI CAynn'nnrTG
Cuts at: 2293 2420 2293
Size: 127 4177
MspA1I CmG'CkG
Cuts at: 598 886 1084 1415 1729 2089 2873 3118 598
Size: 288 198 331 314 360 784 245 1784
Fragments arranged by size:
1784 784 360 331 314 288 245 198
NaeI GCC'GGC
Cuts at: 2485 2485
Size: 4304
NarI GG'CG_CC
Cuts at: 1735 1983 1735
Size: 248 4056
NcoI C'CATG_G
Cuts at: 777 777
Size: 4304
NdeI CA'TA_TG
Cuts at: 584 584
Size: 4304
NgoMIV G'CCGG_C
Cuts at: 2483 2483
Size: 4304
NsiI A_TGCA'T
Cuts at: 570 570
Size: 4304
NspI r_CATG'y
Cuts at: 286 1357 1857 3460 286
Size: 1071 500 1603 1130
Fragments arranged by size:
1603 1130 1071 500
PciI A'CATG_T
Cuts at: 282 3456 282
Size: 3174 1130
PflMI CCAn_nnn'nTGG
Cuts at: 204 617 204
Size: 413 3891
PfoI T'CCnGG_A
Cuts at: 197 197
Size: 4304
PmeI GTTT'AAAC
Cuts at: 1303 1303
Size: 4304
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2750 2782 2750
Size: 32 4272
PpuMI rG'GwC_Cy
Cuts at: 201 201
Size: 4304
PstI C_TGCA'G
Cuts at: 885 1351 2036 885
Size: 466 685 3153
Fragments arranged by size:
3153 685 466
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4304
PvuII CAG'CTG
Cuts at: 598 886 1084 1729 2089 598
Size: 288 198 645 360 2813
Fragments arranged by size:
2813 645 360 288 198
RsrII CG'GwC_CG
Cuts at: 2499 2499
Size: 4304
SacI G_AGCT'C
Cuts at: 1318 1318
Size: 4304
SalI G'TCGA_C
Cuts at: 1341 1341
Size: 4304
SapI GCTCTTCn'nnn_
Cuts at: 2327 2537 2327
Size: 210 4094
SbfI CC_TGCA'GG
Cuts at: 1351 1351
Size: 4304
ScaI AGT'ACT
Cuts at: 3821 3821
Size: 4304
SfcI C'TryA_G
Cuts at: 33 881 1151 1347 2032 3000 3191 33
Size: 848 270 196 685 968 191 1146
Fragments arranged by size:
1146 968 848 685 270 196 191
SfoI GGC'GCC
Cuts at: 1736 1984 1736
Size: 248 4056
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4304
SmaI CCC'GGG
Cuts at: 1326 1326
Size: 4304
SmlI C'TyrA_G
Cuts at: 498 1459 2374 3088 3350 498
Size: 961 915 714 262 1452
Fragments arranged by size:
1452 961 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4304
SphI G_CATG'C
Cuts at: 1357 1857 1357
Size: 500 3804
SspI AAT'ATT
Cuts at: 1137 1137
Size: 4304
StuI AGG'CCT
Cuts at: 392 392
Size: 4304
StyI C'CwwG_G
Cuts at: 777 1157 1438 1792 777
Size: 380 281 354 3289
Fragments arranged by size:
3289 380 354 281
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 322 955 1887 2020 2479 2688 322
Size: 633 932 133 459 209 1938
Fragments arranged by size:
1938 932 633 459 209 133
TatI w'GTAC_w
Cuts at: 3819 3819
Size: 4304
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 234 266 3821 3853 234
Size: 32 3555 32 685
Fragments arranged by size:
3555 685 32 32
Tth111I GACn'n_nGTC
Cuts at: 2101 2101
Size: 4304
XbaI T'CTAG_A
Cuts at: 1335 1335
Size: 4304
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 379 1799 379
Size: 1420 2884
XmaI C'CCGG_G
Cuts at: 1324 1324
Size: 4304
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlfI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI BstZ17I BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NsiI NspI PciI PflMI
PfoI PmeI PpiI PpuMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BmgBI
BmtI BsiWI BsrGI BssHII BstEII Bsu36I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NheI NotI NruI PacI PasI PmlI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SwaI XhoI XmnI ZraI