Restriction map for
pF1KB3489
(Circular) (Six-base) MAPSORT of: pF1KB3489.seq Check: 1616 from: 1 to: 4109
pF1KB3489 4109 bp
With 182 enzymes: SgfI *
April 24, 2009 13:02 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 975 975
Size: 4109
AccI GT'mk_AC
Cuts at: 734 1147 734
Size: 413 3696
Acc65I G'GTAC_C
Cuts at: 1125 1125
Size: 4109
AclI AA'CG_TT
Cuts at: 3949 3949
Size: 4109
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1934 2366 3113 3381 1934
Size: 432 747 268 2662
Fragments arranged by size:
2662 747 432 268
AflIII A'CryG_T
Cuts at: 1461 2565 3542 1461
Size: 1104 977 2028
Fragments arranged by size:
2028 1104 977
AgeI A'CCGG_T
Cuts at: 2497 2497
Size: 4109
AleI CACnn'nnGTG
Cuts at: 835 835
Size: 4109
AlwNI CAG_nnn'CTG
Cuts at: 1414 2981 1414
Size: 1567 2542
ApaLI G'TGCA_C
Cuts at: 2879 2879
Size: 4109
ApoI r'AATT_y
Cuts at: 493 891 1113 3462 3718 493
Size: 398 222 2349 256 884
Fragments arranged by size:
2349 884 398 256 222
AseI AT'TA_AT
Cuts at: 20 350 20
Size: 330 3779
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4109
AvaI C'yCGr_G
Cuts at: 199 1129 199
Size: 930 3179
BamHI G'GATC_C
Cuts at: 1134 1134
Size: 4109
BanI G'GyrC_C
Cuts at: 1125 1539 1787 1822 1125
Size: 414 248 35 3412
Fragments arranged by size:
3412 414 248 35
BanII G_rGCy'C
Cuts at: 980 1123 2153 980
Size: 143 1030 2936
Fragments arranged by size:
2936 1030 143
BbeI G_GCGC'C
Cuts at: 1543 1791 1543
Size: 248 3861
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 237 271 90
Size: 34 113 34 3928
Fragments arranged by size:
3928 113 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2007 2768 4109 2007
Size: 761 1341 2007
Fragments arranged by size:
2007 1341 761
BclI T'GATC_A
Cuts at: 575 1629 575
Size: 1054 3055
BglI GCCn_nnn'nGGC
Cuts at: 602 602
Size: 4109
BglII A'GATC_T
Cuts at: 501 1624 3661 501
Size: 1123 2037 949
Fragments arranged by size:
2037 1123 949
BlpI GC'TnA_GC
Cuts at: 1221 1221
Size: 4109
Bme1580I G_kGCm'C
Cuts at: 1734 1827 2883 1734
Size: 93 1056 2960
Fragments arranged by size:
2960 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 451 1321 1402 1737 3881 451
Size: 870 81 335 2144 679
Fragments arranged by size:
2144 870 679 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2476 2476
Size: 4109
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 938 964 938
Size: 26 4083
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 377 1285 2164 2656 2954 377
Size: 908 879 492 298 1532
Fragments arranged by size:
1532 908 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 820 3813 820
Size: 2993 1116
BsaAI yAC'GTr
Cuts at: 1462 1462
Size: 4109
BsaBI GATnn'nnATC
Cuts at: 6 1623 3660 6
Size: 1617 2037 455
Fragments arranged by size:
2037 1617 455
BsaHI Gr'CG_yC
Cuts at: 1540 1788 2490 3986 1540
Size: 248 702 1496 1663
Fragments arranged by size:
1663 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1298 1819 2497 2771 2918 3329 1298
Size: 521 678 274 147 411 2078
Fragments arranged by size:
2078 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 562 1534 2292 2869 3365 562
Size: 972 758 577 496 1306
Fragments arranged by size:
1306 972 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 491 491
Size: 4109
BsiEI CG_ry'CG
Cuts at: 104 1651 1697 2905 104
Size: 1547 46 1208 1308
Fragments arranged by size:
1547 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1123 1901 2091 2883 1123
Size: 778 190 792 2349
Fragments arranged by size:
2349 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 980 1123 1734 1827 1901 2091 2153 2883 980
Size: 143 611 93 74 190 62 730 2206
Fragments arranged by size:
2206 730 611 190 143 93 74 62
BspEI T'CCGG_A
Cuts at: 3329 3329
Size: 4109
BspHI T'CATG_A
Cuts at: 3285 3285
Size: 4109
BspMI ACCTGCnnnn'nnnn_
Cuts at: 975 1159 1373 1675 2056 975
Size: 184 214 302 381 3028
Fragments arranged by size:
3028 381 302 214 184
BsrBI CCG'CTC
Cuts at: 50 171 2401 2455 3939 4104 50
Size: 121 2230 54 1484 165 55
Fragments arranged by size:
2230 1484 165 121 55 54
BsrDI GCAATG_nn'
Cuts at: 248 2021 248
Size: 1773 2336
BsrFI r'CCGG_y
Cuts at: 2107 2288 2497 2107
Size: 181 209 3719
Fragments arranged by size:
3719 209 181
BsrGI T'GTAC_A
Cuts at: 155 155
Size: 4109
BssSI C'ACGA_G
Cuts at: 133 2380 2738 133
Size: 2247 358 1504
Fragments arranged by size:
2247 1504 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1486 1486
Size: 4109
BstBI TT'CG_AA
Cuts at: 2470 2470
Size: 4109
BstEII G'GTnAC_C
Cuts at: 990 990
Size: 4109
BstYI r'GATC_y
Cuts at: 1 501 1134 1601 1624 1959 2205 3217 3303
Size: 500 633 467 23 335 246 1012 86
Cuts at: 3303 3315 3661 1
Size: 12 346 449
Fragments arranged by size:
1012 633 500 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 995 995
Size: 4109
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2059 2240 2403 2059
Size: 181 163 3765
Fragments arranged by size:
3765 181 163
BtsI GCAGTG_nn'
Cuts at: 488 1352 488
Size: 864 3245
DraI TTT'AAA
Cuts at: 1108 1108
Size: 4109
DrdI GACnn_nn'nnGTC
Cuts at: 1815 2673 1815
Size: 858 3251
EaeI y'GGCC_r
Cuts at: 857 1694 1868 2259 2286 3631 857
Size: 837 174 391 27 1345 1335
Fragments arranged by size:
1345 1335 837 391 174 27
EagI C'GGCC_G
Cuts at: 1694 1694
Size: 4109
EarI CTCTTCn'nnn_
Cuts at: 2132 2342 2132
Size: 210 3899
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2317 2627 2773 3919 2317
Size: 310 146 1146 2507
Fragments arranged by size:
2507 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1121 1121
Size: 4109
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 938 964 1934 2366 3113 3381 938
Size: 26 970 432 747 268 1666
Fragments arranged by size:
1666 970 747 432 268 26
EcoNI CCTnn'n_nnAGG
Cuts at: 522 522
Size: 4109
EcoO109I rG'GnC_Cy
Cuts at: 1248 1248
Size: 4109
EcoRI G'AATT_C
Cuts at: 1113 1113
Size: 4109
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2128 2128
Size: 4109
FspI TGC'GCA
Cuts at: 1890 1890
Size: 4109
HaeII r_GCGC'y
Cuts at: 597 1543 1791 2813 3792 597
Size: 946 248 1022 979 914
Fragments arranged by size:
1022 979 946 914 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 817 849 1951 1981 1983 2013 817
Size: 32 1102 30 2 30 2913
Fragments arranged by size:
2913 1102 32 30 30 2
HincII GTy'rAC
Cuts at: 1148 1148
Size: 4109
HindIII A'AGCT_T
Cuts at: 701 701
Size: 4109
KasI G'GCGC_C
Cuts at: 1539 1787 1539
Size: 248 3861
KpnI G_GTAC'C
Cuts at: 1129 1129
Size: 4109
MluI A'CGCG_T
Cuts at: 3542 3542
Size: 4109
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2780 2964 2780
Size: 184 3925
MscI TGG'CCA
Cuts at: 859 1870 859
Size: 1011 3098
MslI CAynn'nnrTG
Cuts at: 583 835 837 2098 2225 583
Size: 252 2 1261 127 2467
Fragments arranged by size:
2467 1261 252 127 2
MspA1I CmG'CkG
Cuts at: 1220 1534 1894 2907 3152 1220
Size: 314 360 1013 245 2177
Fragments arranged by size:
2177 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2290 2290
Size: 4109
NarI GG'CG_CC
Cuts at: 1540 1788 1540
Size: 248 3861
NcoI C'CATG_G
Cuts at: 995 995
Size: 4109
NgoMIV G'CCGG_C
Cuts at: 2288 2288
Size: 4109
NspI r_CATG'y
Cuts at: 211 459 927 1162 1662 2569 211
Size: 248 468 235 500 907 1751
Fragments arranged by size:
1751 907 500 468 248 235
PciI A'CATG_T
Cuts at: 2565 2565
Size: 4109
PflMI CCAn_nnn'nTGG
Cuts at: 987 987
Size: 4109
PmeI GTTT'AAAC
Cuts at: 1108 1108
Size: 4109
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3248 3280 3248
Size: 32 4077
PsiI TTA'TAA
Cuts at: 283 283
Size: 4109
PstI C_TGCA'G
Cuts at: 320 1156 1841 320
Size: 836 685 2588
Fragments arranged by size:
2588 836 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4109
PvuII CAG'CTG
Cuts at: 1534 1894 1534
Size: 360 3749
RsrII CG'GwC_CG
Cuts at: 2304 2304
Size: 4109
SacI G_AGCT'C
Cuts at: 1123 1123
Size: 4109
SalI G'TCGA_C
Cuts at: 1146 1146
Size: 4109
SapI GCTCTTCn'nnn_
Cuts at: 2132 2342 2132
Size: 210 3899
SbfI CC_TGCA'GG
Cuts at: 1156 1156
Size: 4109
ScaI AGT'ACT
Cuts at: 3626 3626
Size: 4109
SfcI C'TryA_G
Cuts at: 33 316 1152 1837 2830 3021 33
Size: 283 836 685 993 191 1121
Fragments arranged by size:
1121 993 836 685 283 191
SfoI GGC'GCC
Cuts at: 1541 1789 1541
Size: 248 3861
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4109
SmaI CCC'GGG
Cuts at: 201 1131 201
Size: 930 3179
SmlI C'TyrA_G
Cuts at: 392 1264 2179 2671 2933 392
Size: 872 915 492 262 1568
Fragments arranged by size:
1568 915 872 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4109
SphI G_CATG'C
Cuts at: 1162 1662 1162
Size: 500 3609
StyI C'CwwG_G
Cuts at: 906 995 1029 1243 1597 906
Size: 89 34 214 354 3418
Fragments arranged by size:
3418 354 214 89 34
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1692 1825 2284 2493 1692
Size: 133 459 209 3308
Fragments arranged by size:
3308 459 209 133
TatI w'GTAC_w
Cuts at: 155 3624 155
Size: 3469 640
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 467 499 3626 3658 467
Size: 32 3127 32 918
Fragments arranged by size:
3127 918 32 32
Tth111I GACn'n_nGTC
Cuts at: 1906 1906
Size: 4109
XbaI T'CTAG_A
Cuts at: 1140 1140
Size: 4109
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 690 1604 114
Size: 576 914 2619
Fragments arranged by size:
2619 914 576
XmaI C'CCGG_G
Cuts at: 199 1129 199
Size: 930 3179
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PflMI PmeI PpiI PsiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI ApaI AscI
AvrII BaeI BbsI BbvCI BfrBI BmgBI BmtI Bpu10I
BsaXI BseRI BsiWI BsmI BsmBI BssHII BstXI BstZ17I
Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PfoI PmlI PpuMI PshAI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI