Restriction map for
pF1KB3488
(Circular) (Six-base) MAPSORT of: pF1KB3488.seq Check: 5087 from: 1 to: 4163
pF1KB3488 4163 bp
With 182 enzymes: SgfI *
April 24, 2009 13:02 ..
AccI GT'mk_AC
Cuts at: 1201 1201
Size: 4163
Acc65I G'GTAC_C
Cuts at: 1179 1179
Size: 4163
AclI AA'CG_TT
Cuts at: 719 4003 719
Size: 3284 879
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1147 1988 2420 3167 3435 1147
Size: 841 432 747 268 1875
Fragments arranged by size:
1875 841 747 432 268
AflIII A'CryG_T
Cuts at: 1515 2619 3596 1515
Size: 1104 977 2082
Fragments arranged by size:
2082 1104 977
AgeI A'CCGG_T
Cuts at: 2551 2551
Size: 4163
AleI CACnn'nnGTG
Cuts at: 317 317
Size: 4163
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 657 689 657
Size: 32 4131
AlwNI CAG_nnn'CTG
Cuts at: 1033 1468 3035 1033
Size: 435 1567 2161
Fragments arranged by size:
2161 1567 435
ApaLI G'TGCA_C
Cuts at: 2933 2933
Size: 4163
ApoI r'AATT_y
Cuts at: 450 1167 3516 3772 450
Size: 717 2349 256 841
Fragments arranged by size:
2349 841 717 256
AseI AT'TA_AT
Cuts at: 20 587 20
Size: 567 3596
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4163
AvaI C'yCGr_G
Cuts at: 1183 1183
Size: 4163
AvrII C'CTAG_G
Cuts at: 681 681
Size: 4163
BamHI G'GATC_C
Cuts at: 1188 1188
Size: 4163
BanI G'GyrC_C
Cuts at: 1179 1593 1841 1876 1179
Size: 414 248 35 3466
Fragments arranged by size:
3466 414 248 35
BanII G_rGCy'C
Cuts at: 1177 2207 1177
Size: 1030 3133
BbeI G_GCGC'C
Cuts at: 1597 1845 1597
Size: 248 3915
BbvCI CC'TCA_GC
Cuts at: 1102 1102
Size: 4163
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 325 359 325
Size: 34 4129
BciVI GTATCCnnnnn_n'
Cuts at: 2061 2822 4163 2061
Size: 761 1341 2061
Fragments arranged by size:
2061 1341 761
BclI T'GATC_A
Cuts at: 884 899 1683 884
Size: 15 784 3364
Fragments arranged by size:
3364 784 15
BglII A'GATC_T
Cuts at: 1678 3715 1678
Size: 2037 2126
BlpI GC'TnA_GC
Cuts at: 1275 1275
Size: 4163
Bme1580I G_kGCm'C
Cuts at: 1788 1881 2937 1788
Size: 93 1056 3014
Fragments arranged by size:
3014 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1375 1456 1791 3935 1375
Size: 81 335 2144 1603
Fragments arranged by size:
2144 1603 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2530 2530
Size: 4163
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 539 1010 1055 539
Size: 471 45 3647
Fragments arranged by size:
3647 471 45
Bpu10I CC'TnA_GC
Cuts at: 1102 1102
Size: 4163
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1339 2218 2710 3008 1339
Size: 879 492 298 2494
Fragments arranged by size:
2494 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3867 3867
Size: 4163
BsaAI yAC'GTr
Cuts at: 1516 1516
Size: 4163
BsaBI GATnn'nnATC
Cuts at: 6 1677 3714 6
Size: 1671 2037 455
Fragments arranged by size:
2037 1671 455
BsaHI Gr'CG_yC
Cuts at: 1594 1842 2544 4040 1594
Size: 248 702 1496 1717
Fragments arranged by size:
1717 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1352 1873 2551 2825 2972 3383 1352
Size: 521 678 274 147 411 2132
Fragments arranged by size:
2132 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 154 184 902 932 154
Size: 30 718 30 3385
Fragments arranged by size:
3385 718 30 30
BseYI C'CCAG_C
Cuts at: 245 1012 1588 2346 2923 3419 245
Size: 767 576 758 577 496 989
Fragments arranged by size:
989 767 758 577 576 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 730 730
Size: 4163
BsiEI CG_ry'CG
Cuts at: 104 1705 1751 2959 104
Size: 1601 46 1208 1308
Fragments arranged by size:
1601 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1177 1955 2145 2937 1177
Size: 778 190 792 2403
Fragments arranged by size:
2403 792 778 190
BsmI GAATG_Cn'
Cuts at: 671 731 671
Size: 60 4103
Bsp1286I G_dGCh'C
Cuts at: 1177 1788 1881 1955 2145 2207 2937 1177
Size: 611 93 74 190 62 730 2403
Fragments arranged by size:
2403 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3383 3383
Size: 4163
BspHI T'CATG_A
Cuts at: 3339 3339
Size: 4163
BspMI ACCTGCnnnn'nnnn_
Cuts at: 859 1213 1427 1729 2110 859
Size: 354 214 302 381 2912
Fragments arranged by size:
2912 381 354 302 214
BsrBI CCG'CTC
Cuts at: 50 2455 2509 3993 4158 50
Size: 2405 54 1484 165 55
Fragments arranged by size:
2405 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 549 731 743 1067 2075 549
Size: 182 12 324 1008 2637
Fragments arranged by size:
2637 1008 324 182 12
BsrFI r'CCGG_y
Cuts at: 627 2161 2342 2551 627
Size: 1534 181 209 2239
Fragments arranged by size:
2239 1534 209 181
BssSI C'ACGA_G
Cuts at: 2434 2792 2434
Size: 358 3805
BstAPI GCAn_nnn'nTGC
Cuts at: 264 1540 264
Size: 1276 2887
BstBI TT'CG_AA
Cuts at: 207 2524 207
Size: 2317 1846
BstXI CCAn_nnnn'nTGG
Cuts at: 1056 1056
Size: 4163
BstYI r'GATC_y
Cuts at: 1 536 1188 1655 1678 2013 2259 3271 3357
Size: 535 652 467 23 335 246 1012 86
Cuts at: 3357 3369 3715 1
Size: 12 346 449
Fragments arranged by size:
1012 652 535 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 294 294
Size: 4163
BtgI C'CryG_G
Cuts at: 107 637 107
Size: 530 3633
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2113 2294 2457 2113
Size: 181 163 3819
Fragments arranged by size:
3819 181 163
BtsI GCAGTG_nn'
Cuts at: 1406 1406
Size: 4163
ClaI AT'CG_AT
Cuts at: 804 804
Size: 4163
DraI TTT'AAA
Cuts at: 1162 1162
Size: 4163
DrdI GACnn_nn'nnGTC
Cuts at: 1869 2727 1869
Size: 858 3305
EaeI y'GGCC_r
Cuts at: 307 1748 1922 2313 2340 3685 307
Size: 1441 174 391 27 1345 785
Fragments arranged by size:
1441 1345 785 391 174 27
EagI C'GGCC_G
Cuts at: 1748 1748
Size: 4163
EarI CTCTTCn'nnn_
Cuts at: 275 515 1106 2186 2396 275
Size: 240 591 1080 210 2042
Fragments arranged by size:
2042 1080 591 240 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2371 2681 2827 3973 2371
Size: 310 146 1146 2561
Fragments arranged by size:
2561 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1175 1175
Size: 4163
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 539 1010 1055 1147 1988 2420 3167 3435 539
Size: 471 45 92 841 432 747 268 1267
Fragments arranged by size:
1267 841 747 471 432 268 92 45
EcoO109I rG'GnC_Cy
Cuts at: 1302 1302
Size: 4163
EcoRI G'AATT_C
Cuts at: 1167 1167
Size: 4163
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2182 2182
Size: 4163
FspI TGC'GCA
Cuts at: 1944 1944
Size: 4163
HaeII r_GCGC'y
Cuts at: 1597 1845 2867 3846 1597
Size: 248 1022 979 1914
Fragments arranged by size:
1914 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 902 934 2005 2035 2037 2067 902
Size: 32 1071 30 2 30 2998
Fragments arranged by size:
2998 1071 32 30 30 2
HincII GTy'rAC
Cuts at: 1202 1202
Size: 4163
KasI G'GCGC_C
Cuts at: 1593 1841 1593
Size: 248 3915
KpnI G_GTAC'C
Cuts at: 1183 1183
Size: 4163
MluI A'CGCG_T
Cuts at: 3596 3596
Size: 4163
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 700 702 957 2834 3018 700
Size: 2 255 1877 184 1845
Fragments arranged by size:
1877 1845 255 184 2
MscI TGG'CCA
Cuts at: 1924 1924
Size: 4163
MslI CAynn'nnrTG
Cuts at: 317 356 524 736 752 925 1054 2152 2279
Size: 39 168 212 16 173 129 1098 127
Cuts at: 2279 317
Size: 2201
Fragments arranged by size:
2201 1098 212 173 168 129 127 39
16
MspA1I CmG'CkG
Cuts at: 167 382 1274 1588 1948 2961 3206 167
Size: 215 892 314 360 1013 245 1124
Fragments arranged by size:
1124 1013 892 360 314 245 215
NaeI GCC'GGC
Cuts at: 2344 2344
Size: 4163
NarI GG'CG_CC
Cuts at: 1594 1842 1594
Size: 248 3915
NcoI C'CATG_G
Cuts at: 107 637 107
Size: 530 3633
NgoMIV G'CCGG_C
Cuts at: 2342 2342
Size: 4163
NspI r_CATG'y
Cuts at: 1216 1716 2623 1216
Size: 500 907 2756
Fragments arranged by size:
2756 907 500
PciI A'CATG_T
Cuts at: 2619 2619
Size: 4163
PflMI CCAn_nnn'nTGG
Cuts at: 1033 1033
Size: 4163
PfoI T'CCnGG_A
Cuts at: 771 1032 771
Size: 261 3902
PmeI GTTT'AAAC
Cuts at: 1162 1162
Size: 4163
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3302 3334 3302
Size: 32 4131
PstI C_TGCA'G
Cuts at: 155 387 870 1210 1895 155
Size: 232 483 340 685 2423
Fragments arranged by size:
2423 685 483 340 232
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4163
PvuII CAG'CTG
Cuts at: 167 382 1588 1948 167
Size: 215 1206 360 2382
Fragments arranged by size:
2382 1206 360 215
RsrII CG'GwC_CG
Cuts at: 2358 2358
Size: 4163
SacI G_AGCT'C
Cuts at: 1177 1177
Size: 4163
SalI G'TCGA_C
Cuts at: 1200 1200
Size: 4163
SapI GCTCTTCn'nnn_
Cuts at: 1106 2186 2396 1106
Size: 1080 210 2873
Fragments arranged by size:
2873 1080 210
SbfI CC_TGCA'GG
Cuts at: 1210 1210
Size: 4163
ScaI AGT'ACT
Cuts at: 3680 3680
Size: 4163
SfcI C'TryA_G
Cuts at: 33 151 383 866 1206 1891 2884 3075 33
Size: 118 232 483 340 685 993 191 1121
Fragments arranged by size:
1121 993 685 483 340 232 191 118
SfoI GGC'GCC
Cuts at: 1595 1843 1595
Size: 248 3915
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4163
SmaI CCC'GGG
Cuts at: 1185 1185
Size: 4163
SmlI C'TyrA_G
Cuts at: 1318 2233 2725 2987 1318
Size: 915 492 262 2494
Fragments arranged by size:
2494 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4163
SphI G_CATG'C
Cuts at: 1216 1716 1216
Size: 500 3663
StyI C'CwwG_G
Cuts at: 107 637 681 1297 1651 107
Size: 530 44 616 354 2619
Fragments arranged by size:
2619 616 530 354 44
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 276 1746 1879 2338 2547 276
Size: 1470 133 459 209 1892
Fragments arranged by size:
1892 1470 459 209 133
TatI w'GTAC_w
Cuts at: 3678 3678
Size: 4163
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 154 186 1014 1046 3680 3712 154
Size: 32 828 32 2634 32 605
Fragments arranged by size:
2634 828 605 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1960 1960
Size: 4163
XbaI T'CTAG_A
Cuts at: 1194 1194
Size: 4163
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 644 969 1658 644
Size: 325 689 3149
Fragments arranged by size:
3149 689 325
XmaI C'CCGG_G
Cuts at: 1183 1183
Size: 4163
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI
BanI BanII BbeI BbvCI BcgI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI
PfoI PmeI PpiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI ApaI AscI
BaeI BbsI BfrBI BglI BmgBI BmtI BseRI BsiWI
BsmBI BsrGI BssHII BstEII BstZ17I CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI