Restriction map for
pF1KB3487
(Circular) (Six-base) MAPSORT of: pF1KB3487.seq Check: 6319 from: 1 to: 5105
pF1KB3487 5105 bp
With 182 enzymes: SgfI *
April 24, 2009 13:02 ..
AatII G_ACGT'C
Cuts at: 1292 1292
Size: 5105
AccI GT'mk_AC
Cuts at: 1199 2143 1199
Size: 944 4161
Acc65I G'GTAC_C
Cuts at: 2121 2121
Size: 5105
AclI AA'CG_TT
Cuts at: 4945 4945
Size: 5105
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 617 882 1774 2930 3362 3715 4377 617
Size: 265 892 1156 432 353 662 1345
Fragments arranged by size:
1345 1156 892 662 432 353 265
AfeI AGC'GCT
Cuts at: 144 144
Size: 5105
AflIII A'CryG_T
Cuts at: 2457 4257 4538 2457
Size: 1800 281 3024
Fragments arranged by size:
3024 1800 281
AhdI GACnn_n'nnGTC
Cuts at: 1119 1119
Size: 5105
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1688 1688
Size: 5105
AlwNI CAG_nnn'CTG
Cuts at: 1371 1818 2410 3848 1371
Size: 447 592 1438 2628
Fragments arranged by size:
2628 1438 592 447
ApaLI G'TGCA_C
Cuts at: 3943 3943
Size: 5105
ApoI r'AATT_y
Cuts at: 289 2109 4458 4714 289
Size: 1820 2349 256 680
Fragments arranged by size:
2349 1820 680 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5105
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5105
AvaI C'yCGr_G
Cuts at: 197 1462 2125 197
Size: 1265 663 3177
Fragments arranged by size:
3177 1265 663
BamHI G'GATC_C
Cuts at: 2130 2130
Size: 5105
BanI G'GyrC_C
Cuts at: 149 1450 2121 2535 2783 2818 149
Size: 1301 671 414 248 35 2436
Fragments arranged by size:
2436 1301 671 414 248 35
BanII G_rGCy'C
Cuts at: 496 2119 3149 496
Size: 1623 1030 2452
Fragments arranged by size:
2452 1623 1030
BbeI G_GCGC'C
Cuts at: 153 2539 2787 153
Size: 2386 248 2471
Fragments arranged by size:
2471 2386 248
BbsI GAAGACnn'nnnn_
Cuts at: 1762 1898 1762
Size: 136 4969
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 649 683 649
Size: 34 5071
BciVI GTATCCnnnnn_n'
Cuts at: 281 3003 4059 5105 281
Size: 2722 1056 1046 281
Fragments arranged by size:
2722 1056 1046 281
BclI T'GATC_A
Cuts at: 944 2625 944
Size: 1681 3424
BglI GCCn_nnn'nGGC
Cuts at: 1250 1250
Size: 5105
BglII A'GATC_T
Cuts at: 2620 4657 2620
Size: 2037 3068
BlpI GC'TnA_GC
Cuts at: 139 2217 139
Size: 2078 3027
Bme1580I G_kGCm'C
Cuts at: 1406 1512 2730 2823 3947 1406
Size: 106 1218 93 1124 2564
Fragments arranged by size:
2564 1218 1124 106 93
BmgBI CAC'GTC
Cuts at: 908 1197 908
Size: 289 4816
BmrI ACTGGGnnnn_n'
Cuts at: 2018 2317 2398 2733 4877 2018
Size: 299 81 335 2144 2246
Fragments arranged by size:
2246 2144 335 299 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1075 3472 1075
Size: 2397 2708
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 335 483 1174 1208 1559 1825 1992 335
Size: 148 691 34 351 266 167 3448
Fragments arranged by size:
3448 691 351 266 167 148 34
Bpu10I CC'TnA_GC
Cuts at: 1513 1513
Size: 5105
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 625 2281 3160 3874 4172 625
Size: 1656 879 714 298 1558
Fragments arranged by size:
1656 1558 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 734 1733 1792 4809 734
Size: 999 59 3017 1030
Fragments arranged by size:
3017 1030 999 59
BsaAI yAC'GTr
Cuts at: 1380 2458 1380
Size: 1078 4027
BsaBI GATnn'nnATC
Cuts at: 6 2619 4656 6
Size: 2613 2037 455
Fragments arranged by size:
2613 2037 455
BsaHI Gr'CG_yC
Cuts at: 150 1120 1289 2536 2784 3486 4982 150
Size: 970 169 1247 248 702 1496 273
Fragments arranged by size:
1496 1247 970 702 273 248 169
BsaWI w'CCGG_w
Cuts at: 2294 2815 3493 3904 4051 4325 2294
Size: 521 678 411 147 274 3074
Fragments arranged by size:
3074 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1532 1562 1909 1939 1532
Size: 30 347 30 4698
Fragments arranged by size:
4698 347 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 466 1978 466
Size: 1512 3593
BseYI C'CCAG_C
Cuts at: 504 1570 2530 3288 3953 4361 504
Size: 1066 960 758 665 408 1248
Fragments arranged by size:
1248 1066 960 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 888 1195 888
Size: 307 4798
BsiEI CG_ry'CG
Cuts at: 104 1144 1767 2647 2693 3923 104
Size: 1040 623 880 46 1230 1286
Fragments arranged by size:
1286 1230 1040 880 623 46
BsiHKAI G_wGCw'C
Cuts at: 1970 2119 2897 3087 3947 1970
Size: 149 778 190 860 3128
Fragments arranged by size:
3128 860 778 190 149
BsmI GAATG_Cn'
Cuts at: 1055 1343 1055
Size: 288 4817
Bsp1286I G_dGCh'C
Cuts at: 496 1406 1512 1970 2119 2730 2823 2897 3087
Size: 910 106 458 149 611 93 74 190
Cuts at: 3087 3149 3947 496
Size: 62 798 1654
Fragments arranged by size:
1654 910 798 611 458 190 149 106
93 74 62
BspHI T'CATG_A
Cuts at: 285 1586 3537 285
Size: 1301 1951 1853
Fragments arranged by size:
1951 1853 1301
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1211 1356 2155 2369 2671 3052 1211
Size: 145 799 214 302 381 3264
Fragments arranged by size:
3264 799 381 302 214 145
BsrBI CCG'CTC
Cuts at: 50 3397 3451 4935 5100 50
Size: 3347 54 1484 165 55
Fragments arranged by size:
3347 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3017 3017
Size: 5105
BsrFI r'CCGG_y
Cuts at: 3103 3284 3103
Size: 181 4924
BssHII G'CGCG_C
Cuts at: 1877 1877
Size: 5105
BssSI C'ACGA_G
Cuts at: 3376 4084 3376
Size: 708 4397
BstAPI GCAn_nnn'nTGC
Cuts at: 2482 2482
Size: 5105
BstBI TT'CG_AA
Cuts at: 3466 3466
Size: 5105
BstYI r'GATC_y
Cuts at: 1 774 1157 2130 2597 2620 2955 3201 3507
Size: 773 383 973 467 23 335 246 306
Cuts at: 3507 3519 3605 4657 1
Size: 12 86 1052 449
Fragments arranged by size:
1052 973 773 467 449 383 335 306
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1372 1372
Size: 5105
BtgI C'CryG_G
Cuts at: 554 801 554
Size: 247 4858
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 795 826 3055 3236 3399 795
Size: 31 2229 181 163 2501
Fragments arranged by size:
2501 2229 181 163 31
BtsI GCAGTG_nn'
Cuts at: 305 2348 305
Size: 2043 3062
DraI TTT'AAA
Cuts at: 2104 2104
Size: 5105
DrdI GACnn_nn'nnGTC
Cuts at: 1489 2811 4155 1489
Size: 1322 1344 2439
Fragments arranged by size:
2439 1344 1322
EaeI y'GGCC_r
Cuts at: 161 172 696 938 1235 1386 2690 2864 3255
Size: 11 524 242 297 151 1304 174 391
Cuts at: 3255 3282 4627 161
Size: 27 1345 639
Fragments arranged by size:
1345 1304 639 524 391 297 242 174
151 27 11
EagI C'GGCC_G
Cuts at: 2690 2690
Size: 5105
EarI CTCTTCn'nnn_
Cuts at: 1164 1517 3128 3338 1164
Size: 353 1611 210 2931
Fragments arranged by size:
2931 1611 353 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3313 4055 4201 4915 3313
Size: 742 146 714 3503
Fragments arranged by size:
3503 742 714 146
EcoICRI GAG'CTC
Cuts at: 2117 2117
Size: 5105
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 335 483 617 882 1174 1208 1559 1774 1825
Size: 148 134 265 292 34 351 215 51
Cuts at: 1825 1992 2930 3362 3715 4377 335
Size: 167 938 432 353 662 1063
Fragments arranged by size:
1063 938 662 432 353 351 292 265
215 167 148 134 51 34
EcoNI CCTnn'n_nnAGG
Cuts at: 349 349
Size: 5105
EcoO109I rG'GnC_Cy
Cuts at: 123 543 972 1113 1368 2244 123
Size: 420 429 141 255 876 2984
Fragments arranged by size:
2984 876 429 420 255 141
EcoRI G'AATT_C
Cuts at: 2109 2109
Size: 5105
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1974 3124 1974
Size: 1150 3955
FspI TGC'GCA
Cuts at: 2886 2886
Size: 5105
HaeII r_GCGC'y
Cuts at: 146 153 160 314 2539 2787 4017 4788 146
Size: 7 7 154 2225 248 1230 771 463
Fragments arranged by size:
2225 1230 771 463 248 154 7 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 623 655 817 849 1105 1120 1137 1152 1532
Size: 32 162 32 256 15 17 15 380
Cuts at: 1532 1564 2024 2056 2947 2977 2979 3009 623
Size: 32 460 32 891 30 2 30 2719
Fragments arranged by size:
2719 891 460 380 256 162 32 32
32 32 30 30 17 15 15 2
HincII GTy'rAC
Cuts at: 2144 2144
Size: 5105
KasI G'GCGC_C
Cuts at: 149 2535 2783 149
Size: 2386 248 2471
Fragments arranged by size:
2471 2386 248
KpnI G_GTAC'C
Cuts at: 2125 2125
Size: 5105
MluI A'CGCG_T
Cuts at: 4538 4538
Size: 5105
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 521 3864 4048 521
Size: 3343 184 1578
Fragments arranged by size:
3343 1578 184
MscI TGG'CCA
Cuts at: 163 174 698 1237 1388 2866 163
Size: 11 524 539 151 1478 2402
Fragments arranged by size:
2402 1478 539 524 151 11
MslI CAynn'nnrTG
Cuts at: 940 1382 3094 3221 940
Size: 442 1712 127 2824
Fragments arranged by size:
2824 1712 442 127
MspA1I CmG'CkG
Cuts at: 902 1209 1233 2216 2530 2890 3674 3919 902
Size: 307 24 983 314 360 784 245 2088
Fragments arranged by size:
2088 983 784 360 314 307 245 24
NaeI GCC'GGC
Cuts at: 3286 3286
Size: 5105
NarI GG'CG_CC
Cuts at: 150 2536 2784 150
Size: 2386 248 2471
Fragments arranged by size:
2471 2386 248
NgoMIV G'CCGG_C
Cuts at: 3284 3284
Size: 5105
NruI TCG'CGA
Cuts at: 1108 1108
Size: 5105
NspI r_CATG'y
Cuts at: 374 582 1508 1653 1885 2158 2658 4261 374
Size: 208 926 145 232 273 500 1603 1218
Fragments arranged by size:
1603 1218 926 500 273 232 208 145
PciI A'CATG_T
Cuts at: 4257 4257
Size: 5105
PflMI CCAn_nnn'nTGG
Cuts at: 170 1570 170
Size: 1400 3705
PmeI GTTT'AAAC
Cuts at: 2104 2104
Size: 5105
PmlI CAC'GTG
Cuts at: 1380 1380
Size: 5105
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1628 1660 3551 3583 1628
Size: 32 1891 32 3150
Fragments arranged by size:
3150 1891 32 32
PpuMI rG'GwC_Cy
Cuts at: 972 1113 1368 972
Size: 141 255 4709
Fragments arranged by size:
4709 255 141
PsiI TTA'TAA
Cuts at: 1331 1331
Size: 5105
PstI C_TGCA'G
Cuts at: 1208 1814 1838 2152 2837 1208
Size: 606 24 314 685 3476
Fragments arranged by size:
3476 685 606 314 24
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5105
PvuII CAG'CTG
Cuts at: 902 1209 1233 2530 2890 902
Size: 307 24 1297 360 3117
Fragments arranged by size:
3117 1297 360 307 24
RsrII CG'GwC_CG
Cuts at: 3300 3300
Size: 5105
SacI G_AGCT'C
Cuts at: 2119 2119
Size: 5105
SalI G'TCGA_C
Cuts at: 2142 2142
Size: 5105
SapI GCTCTTCn'nnn_
Cuts at: 3128 3338 3128
Size: 210 4895
SbfI CC_TGCA'GG
Cuts at: 2152 2152
Size: 5105
ScaI AGT'ACT
Cuts at: 4622 4622
Size: 5105
SexAI A'CCwGG_T
Cuts at: 394 1484 1790 394
Size: 1090 306 3709
Fragments arranged by size:
3709 1090 306
SfcI C'TryA_G
Cuts at: 33 1204 1810 1834 2148 2833 3801 3992 33
Size: 1171 606 24 314 685 968 191 1146
Fragments arranged by size:
1171 1146 968 685 606 314 191 24
SfoI GGC'GCC
Cuts at: 151 2537 2785 151
Size: 2386 248 2471
Fragments arranged by size:
2471 2386 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5105
SmaI CCC'GGG
Cuts at: 2127 2127
Size: 5105
SmlI C'TyrA_G
Cuts at: 640 2260 3175 3889 4151 640
Size: 1620 915 714 262 1594
Fragments arranged by size:
1620 1594 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5105
SphI G_CATG'C
Cuts at: 582 1653 1885 2158 2658 582
Size: 1071 232 273 500 3029
Fragments arranged by size:
3029 1071 500 273 232
StuI AGG'CCT
Cuts at: 959 1536 959
Size: 577 4528
StyI C'CwwG_G
Cuts at: 164 770 2239 2593 164
Size: 606 1469 354 2676
Fragments arranged by size:
2676 1469 606 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1130 2688 2821 3280 3489 1130
Size: 1558 133 459 209 2746
Fragments arranged by size:
2746 1558 459 209 133
TatI w'GTAC_w
Cuts at: 4620 4620
Size: 5105
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1972 2004 4622 4654 1972
Size: 32 2618 32 2423
Fragments arranged by size:
2618 2423 32 32
Tth111I GACn'n_nGTC
Cuts at: 2902 2902
Size: 5105
XbaI T'CTAG_A
Cuts at: 1123 2136 1123
Size: 1013 4092
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 753 922 2600 753
Size: 169 1678 3258
Fragments arranged by size:
3258 1678 169
XmaI C'CCGG_G
Cuts at: 2125 2125
Size: 5105
XmnI GAAnn'nnTTC
Cuts at: 1759 1759
Size: 5105
ZraI GAC'GTC
Cuts at: 1290 1290
Size: 5105
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflIII AhdI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstYI
Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NruI NspI
PciI PflMI PmeI PmlI PpiI PpuMI PsiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AflII AgeI AleI AloI ApaI AscI AvrII
BaeI BbvCI BfrBI BmtI BsiWI BsmBI BspEI BsrGI
BstEII BstXI BstZ17I ClaI CspCI DraIII EcoRV FseI
FspAI HindIII HpaI MfeI NcoI NdeI NheI NotI
NsiI PacI PasI PfoI PshAI PspOMI PspXI PsrI
SacII SanDI SfiI SgrAI SnaBI SrfI SspI SwaI
XhoI