Restriction map for pF1KB3483
 (Circular) (Six-base) MAPSORT of: pF1KB3483.seq  Check: 7255  from: 1  to: 3872



pF1KB3483  3872 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:23  ..

AccI GT'mk_AC

Cuts at:    910     910 
   Size:       3872
 
Acc65I G'GTAC_C

Cuts at:    888     888 
   Size:       3872
 
AclI AA'CG_TT

Cuts at:   3712    3712 
   Size:       3872
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1697    2129    2876    3144    1697 
   Size:        432     747     268    2425

  Fragments arranged by size: 

                2425     747     432     268
 
AflIII A'CryG_T

Cuts at:   1224    2328    3305    1224 
   Size:       1104     977    1791

  Fragments arranged by size: 

                1791    1104     977
 
AgeI A'CCGG_T

Cuts at:   2260    2260 
   Size:       3872
 
AlwNI CAG_nnn'CTG

Cuts at:    460    1177    2744     460 
   Size:        717    1567    1588

  Fragments arranged by size: 

                1588    1567     717
 
ApaLI G'TGCA_C

Cuts at:    634    2642     634 
   Size:       2008    1864
 
ApoI r'AATT_y

Cuts at:    876    3225    3481     876 
   Size:       2349     256    1267

  Fragments arranged by size: 

                2349    1267     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3872
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3872
 
AvaI C'yCGr_G

Cuts at:    222     892     222 
   Size:        670    3202
 
BamHI G'GATC_C

Cuts at:    897     897 
   Size:       3872
 
BanI G'GyrC_C

Cuts at:    388     888    1302    1550    1585     388 
   Size:        500     414     248      35    2675

  Fragments arranged by size: 

                2675     500     414     248      35
 
BanII G_rGCy'C

Cuts at:    886    1916     886 
   Size:       1030    2842
 
BbeI G_GCGC'C

Cuts at:   1306    1554    1306 
   Size:        248    3624
 
BciVI GTATCCnnnnn_n'

Cuts at:   1770    2531    3872    1770 
   Size:        761    1341    1770

  Fragments arranged by size: 

                1770    1341     761
 
BclI T'GATC_A

Cuts at:   1392    1392 
   Size:       3872
 
BglI GCCn_nnn'nGGC

Cuts at:    242     485     242 
   Size:        243    3629
 
BglII A'GATC_T

Cuts at:   1387    3424    1387 
   Size:       2037    1835
 
BlpI GC'TnA_GC

Cuts at:    984     984 
   Size:       3872
 
Bme1580I G_kGCm'C

Cuts at:    638    1497    1590    2646     638 
   Size:        859      93    1056    1864

  Fragments arranged by size: 

                1864    1056     859      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    215     750    1084    1165    1500    3644     215 
   Size:        535     334      81     335    2144     443

  Fragments arranged by size: 

                2144     535     443     335     334      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    788    2239     788 
   Size:       1451    2421
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    212     260     212 
   Size:         48    3824
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1048    1927    2419    2717    1048 
   Size:        879     492     298    2203

  Fragments arranged by size: 

                2203     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    700    3576     700 
   Size:       2876     996
 
BsaAI yAC'GTr

Cuts at:   1225    1225 
   Size:       3872
 
BsaBI GATnn'nnATC

Cuts at:      6     748    1386    3423       6 
   Size:        742     638    2037     455

  Fragments arranged by size: 

                2037     742     638     455
 
BsaHI Gr'CG_yC

Cuts at:   1303    1551    2253    3749    1303 
   Size:        248     702    1496    1426

  Fragments arranged by size: 

                1496    1426     702     248
 
BsaWI w'CCGG_w

Cuts at:   1061    1582    2260    2534    2681    3092    1061 
   Size:        521     678     274     147     411    1841

  Fragments arranged by size: 

                1841     678     521     411     274     147
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    804     804 
   Size:       3872
 
BseYI C'CCAG_C

Cuts at:    275     299     323     347     371     460     495    1297    2055 
   Size:         24      24      24      24      89      35     802     758

Cuts at:   2055    2632    3128     275 
   Size:        577     496    1019

  Fragments arranged by size: 

                1019     802     758     577     496      89      35      24
                  24      24      24
 
BsiEI CG_ry'CG

Cuts at:    104    1414    1460    2668     104 
   Size:       1310      46    1208    1308

  Fragments arranged by size: 

                1310    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:    638     886    1664    1854    2646     638 
   Size:        248     778     190     792    1864

  Fragments arranged by size: 

                1864     792     778     248     190
 
Bsp1286I G_dGCh'C

Cuts at:    638     886    1497    1590    1664    1854    1916    2646     638 
   Size:        248     611      93      74     190      62     730    1864

  Fragments arranged by size: 

                1864     730     611     248     190      93      74      62
            
 
BspEI T'CCGG_A

Cuts at:   3092    3092 
   Size:       3872
 
BspHI T'CATG_A

Cuts at:   3048    3048 
   Size:       3872
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    922    1136    1438    1819     922 
   Size:        214     302     381    2975

  Fragments arranged by size: 

                2975     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    2164    2218    3702    3867      50 
   Size:       2114      54    1484     165      55

  Fragments arranged by size: 

                2114    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1784    1784 
   Size:       3872
 
BsrFI r'CCGG_y

Cuts at:   1870    2051    2260    1870 
   Size:        181     209    3482

  Fragments arranged by size: 

                3482     209     181
 
BssSI C'ACGA_G

Cuts at:   2143    2501    2143 
   Size:        358    3514
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1249    1249 
   Size:       3872
 
BstBI TT'CG_AA

Cuts at:   2233    2233 
   Size:       3872
 
BstEII G'GTnAC_C

Cuts at:    672     672 
   Size:       3872
 
BstXI CCAn_nnnn'nTGG

Cuts at:    343     343 
   Size:       3872
 
BstYI r'GATC_y

Cuts at:      1     897    1364    1387    1722    1968    2980    3066    3078 
   Size:        896     467      23     335     246    1012      86      12

Cuts at:   3078    3424       1 
   Size:        346     449

  Fragments arranged by size: 

                1012     896     467     449     346     335     246      86
                  23      12
 
Bsu36I CC'TnA_GG

Cuts at:    260     260 
   Size:       3872
 
BtgI C'CryG_G

Cuts at:    107     336     602     107 
   Size:        229     266    3377

  Fragments arranged by size: 

                3377     266     229
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1822    2003    2166    1822 
   Size:        181     163    3528

  Fragments arranged by size: 

                3528     181     163
 
BtsI GCAGTG_nn'

Cuts at:    473     540    1115     473 
   Size:         67     575    3230

  Fragments arranged by size: 

                3230     575      67
 
DraI TTT'AAA

Cuts at:    871     871 
   Size:       3872
 
DrdI GACnn_nn'nnGTC

Cuts at:   1578    2436    1578 
   Size:        858    3014
 
EaeI y'GGCC_r

Cuts at:    146    1457    1631    2022    2049    3394     146 
   Size:       1311     174     391      27    1345     624

  Fragments arranged by size: 

                1345    1311     624     391     174      27
 
EagI C'GGCC_G

Cuts at:   1457    1457 
   Size:       3872
 
EarI CTCTTCn'nnn_

Cuts at:    814    1895    2105     814 
   Size:       1081     210    2581

  Fragments arranged by size: 

                2581    1081     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2080    2390    2536    3682    2080 
   Size:        310     146    1146    2270

  Fragments arranged by size: 

                2270    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    884     884 
   Size:       3872
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    212     260    1697    2129    2876    3144     212 
   Size:         48    1437     432     747     268     940

  Fragments arranged by size: 

                1437     940     747     432     268      48
 
EcoO109I rG'GnC_Cy

Cuts at:    477    1011     477 
   Size:        534    3338
 
EcoRI G'AATT_C

Cuts at:    876     876 
   Size:       3872
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1891    1891 
   Size:       3872
 
FspI TGC'GCA

Cuts at:   1653    1653 
   Size:       3872
 
HaeII r_GCGC'y

Cuts at:    183    1306    1554    2576    3555     183 
   Size:       1123     248    1022     979     500

  Fragments arranged by size: 

                1123    1022     979     500     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    754     786    1714    1744    1746    1776     754 
   Size:         32     928      30       2      30    2850

  Fragments arranged by size: 

                2850     928      32      30      30       2
 
HincII GTy'rAC

Cuts at:    911     911 
   Size:       3872
 
KasI G'GCGC_C

Cuts at:   1302    1550    1302 
   Size:        248    3624
 
KpnI G_GTAC'C

Cuts at:    892     892 
   Size:       3872
 
MluI A'CGCG_T

Cuts at:   3305    3305 
   Size:       3872
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2543    2727    2543 
   Size:        184    3688
 
MscI TGG'CCA

Cuts at:    148    1633     148 
   Size:       1485    2387
 
MslI CAynn'nnrTG

Cuts at:    293     317     341     365    1861    1988     293 
   Size:         24      24      24    1496     127    2177

  Fragments arranged by size: 

                2177    1496     127      24      24      24
 
MspA1I CmG'CkG

Cuts at:    460     983    1297    1657    2670    2915     460 
   Size:        523     314     360    1013     245    1417

  Fragments arranged by size: 

                1417    1013     523     360     314     245
 
NaeI GCC'GGC

Cuts at:   2053    2053 
   Size:       3872
 
NarI GG'CG_CC

Cuts at:   1303    1551    1303 
   Size:        248    3624
 
NcoI C'CATG_G

Cuts at:    107     336     602     107 
   Size:        229     266    3377

  Fragments arranged by size: 

                3377     266     229
 
NgoMIV G'CCGG_C

Cuts at:   2051    2051 
   Size:       3872
 
NspI r_CATG'y

Cuts at:    495     570     925    1425    2332     495 
   Size:         75     355     500     907    2035

  Fragments arranged by size: 

                2035     907     500     355      75
 
PciI A'CATG_T

Cuts at:   2328    2328 
   Size:       3872
 
PmeI GTTT'AAAC

Cuts at:    871     871 
   Size:       3872
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3011    3043    3011 
   Size:         32    3840
 
PstI C_TGCA'G

Cuts at:    456     919    1604     456 
   Size:        463     685    2724

  Fragments arranged by size: 

                2724     685     463
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3872
 
PvuII CAG'CTG

Cuts at:    460    1297    1657     460 
   Size:        837     360    2675

  Fragments arranged by size: 

                2675     837     360
 
RsrII CG'GwC_CG

Cuts at:   2067    2067 
   Size:       3872
 
SacI G_AGCT'C

Cuts at:    886     886 
   Size:       3872
 
SalI G'TCGA_C

Cuts at:    909     909 
   Size:       3872
 
SapI GCTCTTCn'nnn_

Cuts at:   1895    2105    1895 
   Size:        210    3662
 
SbfI CC_TGCA'GG

Cuts at:    919     919 
   Size:       3872
 
ScaI AGT'ACT

Cuts at:   3389    3389 
   Size:       3872
 
SfcI C'TryA_G

Cuts at:     33     452     594     915    1600    2593    2784      33 
   Size:        419     142     321     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     419     321     191     142
 
SfoI GGC'GCC

Cuts at:   1304    1552    1304 
   Size:        248    3624
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3872
 
SmaI CCC'GGG

Cuts at:    224     894     224 
   Size:        670    3202
 
SmlI C'TyrA_G

Cuts at:   1027    1942    2434    2696    1027 
   Size:        915     492     262    2203

  Fragments arranged by size: 

                2203     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3872
 
SphI G_CATG'C

Cuts at:    925    1425     925 
   Size:        500    3372
 
StuI AGG'CCT

Cuts at:    778     778 
   Size:       3872
 
StyI C'CwwG_G

Cuts at:    107     117     336     480     602     686    1006    1360     107 
   Size:         10     219     144     122      84     320     354    2619

  Fragments arranged by size: 

                2619     354     320     219     144     122      84      10
            
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    406     724     769    1455    1588    2047    2256     406 
   Size:        318      45     686     133     459     209    2022

  Fragments arranged by size: 

                2022     686     459     318     209     133      45
 
TatI w'GTAC_w

Cuts at:    590     611    3387     590 
   Size:         21    2776    1075

  Fragments arranged by size: 

                2776    1075      21
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    812     844    3389    3421     812 
   Size:         32    2545      32    1263

  Fragments arranged by size: 

                2545    1263      32      32
 
Tth111I GACn'n_nGTC

Cuts at:    643    1669     643 
   Size:       1026    2846
 
XbaI T'CTAG_A

Cuts at:    903     903 
   Size:       3872
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1367    1367 
   Size:       3872
 
XmaI C'CCGG_G

Cuts at:    222     892     222 
   Size:        670    3202
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI    AlwNI    ApaLI
     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII     BbeI
    BciVI     BclI     BglI    BglII     BlpI Bme1580I     BmrI     BplI
     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseRI
    BseYI    BsiEI  BsiHKAI Bsp1286I    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI
   Bsu36I     BtgI    BtgZI     BtsI     DraI     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI     FspI
    HaeII    Hin4I   HincII     KasI     KpnI     MluI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI     PciI
     PmeI     PpiI     PstI     PvuI    PvuII    RsrII     SacI     SalI
     SapI     SbfI     ScaI     SfcI     SfoI     SgfI     SmaI     SmlI
     SpeI     SphI     StuI     StyI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AleI     AlfI     AloI
     ApaI     AscI    AvrII     BaeI     BbsI    BbvCI     BcgI    BfrBI
    BmgBI     BmtI   Bpu10I    BsaXI     BsgI    BsiWI     BsmI    BsmBI
    BsrGI   BssHII  BstZ17I     ClaI    CspCI   DraIII    EcoNI    EcoRV
     FseI    FspAI  HindIII     HpaI     MfeI     NdeI     NheI     NotI
     NruI     NsiI     PacI     PasI    PflMI     PfoI     PmlI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI    SacII    SanDI    SexAI
     SfiI    SgrAI    SnaBI     SrfI     SspI     SwaI     XhoI     XmnI
     ZraI
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