(Circular) (Six-base) MAPSORT of: pF1KB3483.seq Check: 7255 from: 1 to: 3872 pF1KB3483 3872 bp With 182 enzymes: SgfI * June 20, 2008 10:23 .. AccI GT'mk_AC Cuts at: 910 910 Size: 3872 Acc65I G'GTAC_C Cuts at: 888 888 Size: 3872 AclI AA'CG_TT Cuts at: 3712 3712 Size: 3872 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1697 2129 2876 3144 1697 Size: 432 747 268 2425 Fragments arranged by size: 2425 747 432 268 AflIII A'CryG_T Cuts at: 1224 2328 3305 1224 Size: 1104 977 1791 Fragments arranged by size: 1791 1104 977 AgeI A'CCGG_T Cuts at: 2260 2260 Size: 3872 AlwNI CAG_nnn'CTG Cuts at: 460 1177 2744 460 Size: 717 1567 1588 Fragments arranged by size: 1588 1567 717 ApaLI G'TGCA_C Cuts at: 634 2642 634 Size: 2008 1864 ApoI r'AATT_y Cuts at: 876 3225 3481 876 Size: 2349 256 1267 Fragments arranged by size: 2349 1267 256 AseI AT'TA_AT Cuts at: 20 20 Size: 3872 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 3872 AvaI C'yCGr_G Cuts at: 222 892 222 Size: 670 3202 BamHI G'GATC_C Cuts at: 897 897 Size: 3872 BanI G'GyrC_C Cuts at: 388 888 1302 1550 1585 388 Size: 500 414 248 35 2675 Fragments arranged by size: 2675 500 414 248 35 BanII G_rGCy'C Cuts at: 886 1916 886 Size: 1030 2842 BbeI G_GCGC'C Cuts at: 1306 1554 1306 Size: 248 3624 BciVI GTATCCnnnnn_n' Cuts at: 1770 2531 3872 1770 Size: 761 1341 1770 Fragments arranged by size: 1770 1341 761 BclI T'GATC_A Cuts at: 1392 1392 Size: 3872 BglI GCCn_nnn'nGGC Cuts at: 242 485 242 Size: 243 3629 BglII A'GATC_T Cuts at: 1387 3424 1387 Size: 2037 1835 BlpI GC'TnA_GC Cuts at: 984 984 Size: 3872 Bme1580I G_kGCm'C Cuts at: 638 1497 1590 2646 638 Size: 859 93 1056 1864 Fragments arranged by size: 1864 1056 859 93 BmrI ACTGGGnnnn_n' Cuts at: 215 750 1084 1165 1500 3644 215 Size: 535 334 81 335 2144 443 Fragments arranged by size: 2144 535 443 335 334 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 788 2239 788 Size: 1451 2421 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 212 260 212 Size: 48 3824 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1048 1927 2419 2717 1048 Size: 879 492 298 2203 Fragments arranged by size: 2203 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 700 3576 700 Size: 2876 996 BsaAI yAC'GTr Cuts at: 1225 1225 Size: 3872 BsaBI GATnn'nnATC Cuts at: 6 748 1386 3423 6 Size: 742 638 2037 455 Fragments arranged by size: 2037 742 638 455 BsaHI Gr'CG_yC Cuts at: 1303 1551 2253 3749 1303 Size: 248 702 1496 1426 Fragments arranged by size: 1496 1426 702 248 BsaWI w'CCGG_w Cuts at: 1061 1582 2260 2534 2681 3092 1061 Size: 521 678 274 147 411 1841 Fragments arranged by size: 1841 678 521 411 274 147 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 804 804 Size: 3872 BseYI C'CCAG_C Cuts at: 275 299 323 347 371 460 495 1297 2055 Size: 24 24 24 24 89 35 802 758 Cuts at: 2055 2632 3128 275 Size: 577 496 1019 Fragments arranged by size: 1019 802 758 577 496 89 35 24 24 24 24 BsiEI CG_ry'CG Cuts at: 104 1414 1460 2668 104 Size: 1310 46 1208 1308 Fragments arranged by size: 1310 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 638 886 1664 1854 2646 638 Size: 248 778 190 792 1864 Fragments arranged by size: 1864 792 778 248 190 Bsp1286I G_dGCh'C Cuts at: 638 886 1497 1590 1664 1854 1916 2646 638 Size: 248 611 93 74 190 62 730 1864 Fragments arranged by size: 1864 730 611 248 190 93 74 62 BspEI T'CCGG_A Cuts at: 3092 3092 Size: 3872 BspHI T'CATG_A Cuts at: 3048 3048 Size: 3872 BspMI ACCTGCnnnn'nnnn_ Cuts at: 922 1136 1438 1819 922 Size: 214 302 381 2975 Fragments arranged by size: 2975 381 302 214 BsrBI CCG'CTC Cuts at: 50 2164 2218 3702 3867 50 Size: 2114 54 1484 165 55 Fragments arranged by size: 2114 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 1784 1784 Size: 3872 BsrFI r'CCGG_y Cuts at: 1870 2051 2260 1870 Size: 181 209 3482 Fragments arranged by size: 3482 209 181 BssSI C'ACGA_G Cuts at: 2143 2501 2143 Size: 358 3514 BstAPI GCAn_nnn'nTGC Cuts at: 1249 1249 Size: 3872 BstBI TT'CG_AA Cuts at: 2233 2233 Size: 3872 BstEII G'GTnAC_C Cuts at: 672 672 Size: 3872 BstXI CCAn_nnnn'nTGG Cuts at: 343 343 Size: 3872 BstYI r'GATC_y Cuts at: 1 897 1364 1387 1722 1968 2980 3066 3078 Size: 896 467 23 335 246 1012 86 12 Cuts at: 3078 3424 1 Size: 346 449 Fragments arranged by size: 1012 896 467 449 346 335 246 86 23 12 Bsu36I CC'TnA_GG Cuts at: 260 260 Size: 3872 BtgI C'CryG_G Cuts at: 107 336 602 107 Size: 229 266 3377 Fragments arranged by size: 3377 266 229 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 1822 2003 2166 1822 Size: 181 163 3528 Fragments arranged by size: 3528 181 163 BtsI GCAGTG_nn' Cuts at: 473 540 1115 473 Size: 67 575 3230 Fragments arranged by size: 3230 575 67 DraI TTT'AAA Cuts at: 871 871 Size: 3872 DrdI GACnn_nn'nnGTC Cuts at: 1578 2436 1578 Size: 858 3014 EaeI y'GGCC_r Cuts at: 146 1457 1631 2022 2049 3394 146 Size: 1311 174 391 27 1345 624 Fragments arranged by size: 1345 1311 624 391 174 27 EagI C'GGCC_G Cuts at: 1457 1457 Size: 3872 EarI CTCTTCn'nnn_ Cuts at: 814 1895 2105 814 Size: 1081 210 2581 Fragments arranged by size: 2581 1081 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 2080 2390 2536 3682 2080 Size: 310 146 1146 2270 Fragments arranged by size: 2270 1146 310 146 EcoICRI GAG'CTC Cuts at: 884 884 Size: 3872 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 212 260 1697 2129 2876 3144 212 Size: 48 1437 432 747 268 940 Fragments arranged by size: 1437 940 747 432 268 48 EcoO109I rG'GnC_Cy Cuts at: 477 1011 477 Size: 534 3338 EcoRI G'AATT_C Cuts at: 876 876 Size: 3872 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 1891 1891 Size: 3872 FspI TGC'GCA Cuts at: 1653 1653 Size: 3872 HaeII r_GCGC'y Cuts at: 183 1306 1554 2576 3555 183 Size: 1123 248 1022 979 500 Fragments arranged by size: 1123 1022 979 500 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 754 786 1714 1744 1746 1776 754 Size: 32 928 30 2 30 2850 Fragments arranged by size: 2850 928 32 30 30 2 HincII GTy'rAC Cuts at: 911 911 Size: 3872 KasI G'GCGC_C Cuts at: 1302 1550 1302 Size: 248 3624 KpnI G_GTAC'C Cuts at: 892 892 Size: 3872 MluI A'CGCG_T Cuts at: 3305 3305 Size: 3872 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 2543 2727 2543 Size: 184 3688 MscI TGG'CCA Cuts at: 148 1633 148 Size: 1485 2387 MslI CAynn'nnrTG Cuts at: 293 317 341 365 1861 1988 293 Size: 24 24 24 1496 127 2177 Fragments arranged by size: 2177 1496 127 24 24 24 MspA1I CmG'CkG Cuts at: 460 983 1297 1657 2670 2915 460 Size: 523 314 360 1013 245 1417 Fragments arranged by size: 1417 1013 523 360 314 245 NaeI GCC'GGC Cuts at: 2053 2053 Size: 3872 NarI GG'CG_CC Cuts at: 1303 1551 1303 Size: 248 3624 NcoI C'CATG_G Cuts at: 107 336 602 107 Size: 229 266 3377 Fragments arranged by size: 3377 266 229 NgoMIV G'CCGG_C Cuts at: 2051 2051 Size: 3872 NspI r_CATG'y Cuts at: 495 570 925 1425 2332 495 Size: 75 355 500 907 2035 Fragments arranged by size: 2035 907 500 355 75 PciI A'CATG_T Cuts at: 2328 2328 Size: 3872 PmeI GTTT'AAAC Cuts at: 871 871 Size: 3872 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3011 3043 3011 Size: 32 3840 PstI C_TGCA'G Cuts at: 456 919 1604 456 Size: 463 685 2724 Fragments arranged by size: 2724 685 463 PvuI CG_AT'CG Cuts at: 104 104 Size: 3872 PvuII CAG'CTG Cuts at: 460 1297 1657 460 Size: 837 360 2675 Fragments arranged by size: 2675 837 360 RsrII CG'GwC_CG Cuts at: 2067 2067 Size: 3872 SacI G_AGCT'C Cuts at: 886 886 Size: 3872 SalI G'TCGA_C Cuts at: 909 909 Size: 3872 SapI GCTCTTCn'nnn_ Cuts at: 1895 2105 1895 Size: 210 3662 SbfI CC_TGCA'GG Cuts at: 919 919 Size: 3872 ScaI AGT'ACT Cuts at: 3389 3389 Size: 3872 SfcI C'TryA_G Cuts at: 33 452 594 915 1600 2593 2784 33 Size: 419 142 321 685 993 191 1121 Fragments arranged by size: 1121 993 685 419 321 191 142 SfoI GGC'GCC Cuts at: 1304 1552 1304 Size: 248 3624 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 3872 SmaI CCC'GGG Cuts at: 224 894 224 Size: 670 3202 SmlI C'TyrA_G Cuts at: 1027 1942 2434 2696 1027 Size: 915 492 262 2203 Fragments arranged by size: 2203 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 3872 SphI G_CATG'C Cuts at: 925 1425 925 Size: 500 3372 StuI AGG'CCT Cuts at: 778 778 Size: 3872 StyI C'CwwG_G Cuts at: 107 117 336 480 602 686 1006 1360 107 Size: 10 219 144 122 84 320 354 2619 Fragments arranged by size: 2619 354 320 219 144 122 84 10 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 406 724 769 1455 1588 2047 2256 406 Size: 318 45 686 133 459 209 2022 Fragments arranged by size: 2022 686 459 318 209 133 45 TatI w'GTAC_w Cuts at: 590 611 3387 590 Size: 21 2776 1075 Fragments arranged by size: 2776 1075 21 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 812 844 3389 3421 812 Size: 32 2545 32 1263 Fragments arranged by size: 2545 1263 32 32 Tth111I GACn'n_nGTC Cuts at: 643 1669 643 Size: 1026 2846 XbaI T'CTAG_A Cuts at: 903 903 Size: 3872 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1367 1367 Size: 3872 XmaI C'CCGG_G Cuts at: 222 892 222 Size: 670 3202 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI PmeI PpiI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AarI AatII AfeI AflII AhdI AleI AlfI AloI ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BmgBI BmtI Bpu10I BsaXI BsgI BsiWI BsmI BsmBI BsrGI BssHII BstZ17I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI NotI NruI NsiI PacI PasI PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI XmnI ZraI