Restriction map for
pF1KB3480
(Circular) (Six-base) MAPSORT of: pF1KB3480.seq Check: 5525 from: 1 to: 4061
pF1KB3480 4061 bp
With 182 enzymes: SgfI *
April 24, 2009 13:02 ..
AatII G_ACGT'C
Cuts at: 897 897
Size: 4061
AccI GT'mk_AC
Cuts at: 1099 1099
Size: 4061
Acc65I G'GTAC_C
Cuts at: 1077 1077
Size: 4061
AclI AA'CG_TT
Cuts at: 1055 3901 1055
Size: 2846 1215
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1886 2318 3065 3333 1886
Size: 432 747 268 2614
Fragments arranged by size:
2614 747 432 268
AflIII A'CryG_T
Cuts at: 410 1413 2517 3494 410
Size: 1003 1104 977 977
Fragments arranged by size:
1104 1003 977 977
AgeI A'CCGG_T
Cuts at: 2449 2449
Size: 4061
AleI CACnn'nnGTG
Cuts at: 650 650
Size: 4061
AlwNI CAG_nnn'CTG
Cuts at: 1366 2933 1366
Size: 1567 2494
ApaLI G'TGCA_C
Cuts at: 2831 2831
Size: 4061
ApoI r'AATT_y
Cuts at: 595 1065 3414 3670 595
Size: 470 2349 256 986
Fragments arranged by size:
2349 986 470 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4061
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4061
AvaI C'yCGr_G
Cuts at: 1081 1081
Size: 4061
BamHI G'GATC_C
Cuts at: 609 1086 609
Size: 477 3584
BanI G'GyrC_C
Cuts at: 530 987 1077 1491 1739 1774 530
Size: 457 90 414 248 35 2817
Fragments arranged by size:
2817 457 414 248 90 35
BanII G_rGCy'C
Cuts at: 245 300 1075 2105 245
Size: 55 775 1030 2201
Fragments arranged by size:
2201 1030 775 55
BbeI G_GCGC'C
Cuts at: 1495 1743 1495
Size: 248 3813
BbvCI CC'TCA_GC
Cuts at: 436 526 436
Size: 90 3971
BciVI GTATCCnnnnn_n'
Cuts at: 1959 2720 4061 1959
Size: 761 1341 1959
Fragments arranged by size:
1959 1341 761
BclI T'GATC_A
Cuts at: 1581 1581
Size: 4061
BglI GCCn_nnn'nGGC
Cuts at: 422 422
Size: 4061
BglII A'GATC_T
Cuts at: 1576 3613 1576
Size: 2037 2024
BlpI GC'TnA_GC
Cuts at: 1173 1173
Size: 4061
Bme1580I G_kGCm'C
Cuts at: 728 990 1686 1779 2835 728
Size: 262 696 93 1056 1954
Fragments arranged by size:
1954 1056 696 262 93
BmrI ACTGGGnnnn_n'
Cuts at: 1273 1354 1689 3833 1273
Size: 81 335 2144 1501
Fragments arranged by size:
2144 1501 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 333 2428 333
Size: 2095 1966
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 247 466 517 247
Size: 219 51 3791
Fragments arranged by size:
3791 219 51
Bpu10I CC'TnA_GC
Cuts at: 436 526 436
Size: 90 3971
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 259 1237 2116 2608 2906 259
Size: 978 879 492 298 1414
Fragments arranged by size:
1414 978 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 112 3765 112
Size: 3653 408
BsaAI yAC'GTr
Cuts at: 413 1414 413
Size: 1001 3060
BsaBI GATnn'nnATC
Cuts at: 6 907 1575 3612 6
Size: 901 668 2037 455
Fragments arranged by size:
2037 901 668 455
BsaHI Gr'CG_yC
Cuts at: 894 1492 1740 2442 3938 894
Size: 598 248 702 1496 1017
Fragments arranged by size:
1496 1017 702 598 248
BsaWI w'CCGG_w
Cuts at: 1250 1771 2449 2723 2870 3281 1250
Size: 521 678 274 147 411 2030
Fragments arranged by size:
2030 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 397 427 1011 1041 397
Size: 30 584 30 3417
Fragments arranged by size:
3417 584 30 30
BseYI C'CCAG_C
Cuts at: 367 727 1486 2244 2821 3317 367
Size: 360 759 758 577 496 1111
Fragments arranged by size:
1111 759 758 577 496 360
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 648 648
Size: 4061
BsiEI CG_ry'CG
Cuts at: 104 1603 1649 2857 104
Size: 1499 46 1208 1308
Fragments arranged by size:
1499 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 263 482 1075 1853 2043 2835 263
Size: 219 593 778 190 792 1489
Fragments arranged by size:
1489 792 778 593 219 190
Bsp1286I G_dGCh'C
Cuts at: 245 263 300 482 728 990 1075 1686 1779
Size: 18 37 182 246 262 85 611 93
Cuts at: 1779 1853 2043 2105 2835 245
Size: 74 190 62 730 1471
Fragments arranged by size:
1471 730 611 262 246 190 182 93
85 74 62 37 18
BspEI T'CCGG_A
Cuts at: 3281 3281
Size: 4061
BspHI T'CATG_A
Cuts at: 3237 3237
Size: 4061
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1111 1325 1627 2008 1111
Size: 214 302 381 3164
Fragments arranged by size:
3164 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2353 2407 3891 4056 50
Size: 2303 54 1484 165 55
Fragments arranged by size:
2303 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 773 1973 773
Size: 1200 2861
BsrFI r'CCGG_y
Cuts at: 2059 2240 2449 2059
Size: 181 209 3671
Fragments arranged by size:
3671 209 181
BssSI C'ACGA_G
Cuts at: 2332 2690 2332
Size: 358 3703
BstAPI GCAn_nnn'nTGC
Cuts at: 1438 1438
Size: 4061
BstBI TT'CG_AA
Cuts at: 2422 2422
Size: 4061
BstXI CCAn_nnnn'nTGG
Cuts at: 114 114
Size: 4061
BstYI r'GATC_y
Cuts at: 1 609 1086 1553 1576 1911 2157 3169 3255
Size: 608 477 467 23 335 246 1012 86
Cuts at: 3255 3267 3613 1
Size: 12 346 449
Fragments arranged by size:
1012 608 477 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 247 247
Size: 4061
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2011 2192 2355 2011
Size: 181 163 3717
Fragments arranged by size:
3717 181 163
BtsI GCAGTG_nn'
Cuts at: 660 1304 660
Size: 644 3417
DraI TTT'AAA
Cuts at: 1060 1060
Size: 4061
DrdI GACnn_nn'nnGTC
Cuts at: 126 1767 2625 126
Size: 1641 858 1562
Fragments arranged by size:
1641 1562 858
EaeI y'GGCC_r
Cuts at: 423 1646 1820 2211 2238 3583 423
Size: 1223 174 391 27 1345 901
Fragments arranged by size:
1345 1223 901 391 174 27
EagI C'GGCC_G
Cuts at: 1646 1646
Size: 4061
EarI CTCTTCn'nnn_
Cuts at: 747 1028 2084 2294 747
Size: 281 1056 210 2514
Fragments arranged by size:
2514 1056 281 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2269 2579 2725 3871 2269
Size: 310 146 1146 2459
Fragments arranged by size:
2459 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1073 1073
Size: 4061
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 247 466 517 1886 2318 3065 3333 247
Size: 219 51 1369 432 747 268 975
Fragments arranged by size:
1369 975 747 432 268 219 51
EcoO109I rG'GnC_Cy
Cuts at: 1200 1200
Size: 4061
EcoRI G'AATT_C
Cuts at: 1065 1065
Size: 4061
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2080 2080
Size: 4061
FspI TGC'GCA
Cuts at: 1842 1842
Size: 4061
HaeII r_GCGC'y
Cuts at: 1495 1743 2765 3744 1495
Size: 248 1022 979 1812
Fragments arranged by size:
1812 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 503 535 1011 1043 1903 1933 1935 1965 503
Size: 32 476 32 860 30 2 30 2599
Fragments arranged by size:
2599 860 476 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1044 1100 1044
Size: 56 4005
KasI G'GCGC_C
Cuts at: 1491 1739 1491
Size: 248 3813
KpnI G_GTAC'C
Cuts at: 1081 1081
Size: 4061
MluI A'CGCG_T
Cuts at: 3494 3494
Size: 4061
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2732 2916 2732
Size: 184 3877
MscI TGG'CCA
Cuts at: 425 1822 425
Size: 1397 2664
MslI CAynn'nnrTG
Cuts at: 112 650 2050 2177 112
Size: 538 1400 127 1996
Fragments arranged by size:
1996 1400 538 127
MspA1I CmG'CkG
Cuts at: 401 539 955 1172 1486 1846 2859 3104 401
Size: 138 416 217 314 360 1013 245 1358
Fragments arranged by size:
1358 1013 416 360 314 245 217 138
NaeI GCC'GGC
Cuts at: 2242 2242
Size: 4061
NarI GG'CG_CC
Cuts at: 1492 1740 1492
Size: 248 3813
NgoMIV G'CCGG_C
Cuts at: 2240 2240
Size: 4061
NspI r_CATG'y
Cuts at: 290 1114 1614 2521 290
Size: 824 500 907 1830
Fragments arranged by size:
1830 907 824 500
PasI CC'CwG_GG
Cuts at: 983 983
Size: 4061
PciI A'CATG_T
Cuts at: 2517 2517
Size: 4061
PflMI CCAn_nnn'nTGG
Cuts at: 326 326
Size: 4061
PfoI T'CCnGG_A
Cuts at: 263 503 263
Size: 240 3821
PmeI GTTT'AAAC
Cuts at: 1060 1060
Size: 4061
PmlI CAC'GTG
Cuts at: 413 413
Size: 4061
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3200 3232 3200
Size: 32 4029
PstI C_TGCA'G
Cuts at: 201 829 1108 1793 201
Size: 628 279 685 2469
Fragments arranged by size:
2469 685 628 279
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4061
PvuII CAG'CTG
Cuts at: 539 955 1486 1846 539
Size: 416 531 360 2754
Fragments arranged by size:
2754 531 416 360
RsrII CG'GwC_CG
Cuts at: 2256 2256
Size: 4061
SacI G_AGCT'C
Cuts at: 1075 1075
Size: 4061
SalI G'TCGA_C
Cuts at: 1098 1098
Size: 4061
SapI GCTCTTCn'nnn_
Cuts at: 747 2084 2294 747
Size: 1337 210 2514
Fragments arranged by size:
2514 1337 210
SbfI CC_TGCA'GG
Cuts at: 1108 1108
Size: 4061
ScaI AGT'ACT
Cuts at: 3578 3578
Size: 4061
SfcI C'TryA_G
Cuts at: 33 197 372 785 806 825 1104 1789 2782
Size: 164 175 413 21 19 279 685 993
Cuts at: 2782 2973 33
Size: 191 1121
Fragments arranged by size:
1121 993 685 413 279 191 175 164
21 19
SfoI GGC'GCC
Cuts at: 1493 1741 1493
Size: 248 3813
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4061
SmaI CCC'GGG
Cuts at: 1083 1083
Size: 4061
SmlI C'TyrA_G
Cuts at: 238 1216 2131 2623 2885 238
Size: 978 915 492 262 1414
Fragments arranged by size:
1414 978 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4061
SphI G_CATG'C
Cuts at: 1114 1614 1114
Size: 500 3561
StyI C'CwwG_G
Cuts at: 1195 1549 1195
Size: 354 3707
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 370 1644 1777 2236 2445 370
Size: 1274 133 459 209 1986
Fragments arranged by size:
1986 1274 459 209 133
TatI w'GTAC_w
Cuts at: 407 3576 407
Size: 3169 892
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 397 429 3578 3610 397
Size: 32 3149 32 848
Fragments arranged by size:
3149 848 32 32
Tth111I GACn'n_nGTC
Cuts at: 1039 1858 1039
Size: 819 3242
XbaI T'CTAG_A
Cuts at: 1092 1092
Size: 4061
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 646 1556 646
Size: 910 3151
XmaI C'CCGG_G
Cuts at: 1081 1081
Size: 4061
ZraI GAC'GTC
Cuts at: 895 895
Size: 4061
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbvCI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AlfI AloI ApaI AscI
AvrII BaeI BbsI BcgI BfrBI BmgBI BmtI BseRI
BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NcoI NdeI NheI NotI NruI NsiI
PacI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI