Restriction map for
pF1KB3479
(Circular) (Six-base) MAPSORT of: pF1KB3479.seq Check: 2686 from: 1 to: 4376
pF1KB3479 4376 bp
With 182 enzymes: SgfI *
April 24, 2009 13:01 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 925 925
Size: 4376
AccI GT'mk_AC
Cuts at: 322 1045 1414 322
Size: 723 369 3284
Fragments arranged by size:
3284 723 369
Acc65I G'GTAC_C
Cuts at: 1392 1392
Size: 4376
AclI AA'CG_TT
Cuts at: 4216 4216
Size: 4376
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2201 2633 3380 3648 2201
Size: 432 747 268 2929
Fragments arranged by size:
2929 747 432 268
AflIII A'CryG_T
Cuts at: 1728 2832 3809 1728
Size: 1104 977 2295
Fragments arranged by size:
2295 1104 977
AgeI A'CCGG_T
Cuts at: 2764 2764
Size: 4376
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 587 587
Size: 4376
AlwNI CAG_nnn'CTG
Cuts at: 403 917 1681 3248 403
Size: 514 764 1567 1531
Fragments arranged by size:
1567 1531 764 514
ApaLI G'TGCA_C
Cuts at: 3146 3146
Size: 4376
ApoI r'AATT_y
Cuts at: 356 1380 3729 3985 356
Size: 1024 2349 256 747
Fragments arranged by size:
2349 1024 747 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4376
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4376
AvaI C'yCGr_G
Cuts at: 1396 1396
Size: 4376
BamHI G'GATC_C
Cuts at: 1401 1401
Size: 4376
BanI G'GyrC_C
Cuts at: 890 1070 1392 1806 2054 2089 890
Size: 180 322 414 248 35 3177
Fragments arranged by size:
3177 414 322 248 180 35
BanII G_rGCy'C
Cuts at: 168 450 1390 2420 168
Size: 282 940 1030 2124
Fragments arranged by size:
2124 1030 940 282
BbeI G_GCGC'C
Cuts at: 1810 2058 1810
Size: 248 4128
BbsI GAAGACnn'nnnn_
Cuts at: 317 317
Size: 4376
BciVI GTATCCnnnnn_n'
Cuts at: 2274 3035 4376 2274
Size: 761 1341 2274
Fragments arranged by size:
2274 1341 761
BclI T'GATC_A
Cuts at: 1896 1896
Size: 4376
BfrBI ATG'CAT
Cuts at: 1258 1258
Size: 4376
BglI GCCn_nnn'nGGC
Cuts at: 868 868
Size: 4376
BglII A'GATC_T
Cuts at: 1891 3928 1891
Size: 2037 2339
BlpI GC'TnA_GC
Cuts at: 788 1488 788
Size: 700 3676
Bme1580I G_kGCm'C
Cuts at: 537 2001 2094 3150 537
Size: 1464 93 1056 1763
Fragments arranged by size:
1763 1464 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1588 1669 2004 4148 1588
Size: 81 335 2144 1816
Fragments arranged by size:
2144 1816 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 680 2743 680
Size: 2063 2313
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 237 755 237
Size: 518 3858
Bpu10I CC'TnA_GC
Cuts at: 141 141
Size: 4376
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 291 1552 2431 2923 3221 291
Size: 1261 879 492 298 1446
Fragments arranged by size:
1446 1261 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4080 4080
Size: 4376
BsaAI yAC'GTr
Cuts at: 1128 1729 1128
Size: 601 3775
BsaBI GATnn'nnATC
Cuts at: 6 1890 3927 6
Size: 1884 2037 455
Fragments arranged by size:
2037 1884 455
BsaHI Gr'CG_yC
Cuts at: 1807 2055 2757 4253 1807
Size: 248 702 1496 1930
Fragments arranged by size:
1930 1496 702 248
BsaWI w'CCGG_w
Cuts at: 542 1565 2086 2764 3038 3185 3596 542
Size: 1023 521 678 274 147 411 1322
Fragments arranged by size:
1322 1023 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 440 440
Size: 4376
BseYI C'CCAG_C
Cuts at: 753 849 894 1114 1801 2559 3136 3632 753
Size: 96 45 220 687 758 577 496 1497
Fragments arranged by size:
1497 758 687 577 496 220 96 45
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 193 557 1243 193
Size: 364 686 3326
Fragments arranged by size:
3326 686 364
BsiEI CG_ry'CG
Cuts at: 104 1918 1964 3172 104
Size: 1814 46 1208 1308
Fragments arranged by size:
1814 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 794 1282 1390 2168 2358 3150 794
Size: 488 108 778 190 792 2020
Fragments arranged by size:
2020 792 778 488 190 108
BsmI GAATG_Cn'
Cuts at: 1106 1106
Size: 4376
Bsp1286I G_dGCh'C
Cuts at: 168 450 537 794 1282 1390 2001 2094 2168
Size: 282 87 257 488 108 611 93 74
Cuts at: 2168 2358 2420 3150 168
Size: 190 62 730 1394
Fragments arranged by size:
1394 730 611 488 282 257 190 108
93 87 74 62
BspEI T'CCGG_A
Cuts at: 3596 3596
Size: 4376
BspHI T'CATG_A
Cuts at: 3552 3552
Size: 4376
BspMI ACCTGCnnnn'nnnn_
Cuts at: 925 1426 1640 1942 2323 925
Size: 501 214 302 381 2978
Fragments arranged by size:
2978 501 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2668 2722 4206 4371 50
Size: 2618 54 1484 165 55
Fragments arranged by size:
2618 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 282 381 1044 2288 282
Size: 99 663 1244 2370
Fragments arranged by size:
2370 1244 663 99
BsrFI r'CCGG_y
Cuts at: 2374 2555 2764 2374
Size: 181 209 3986
Fragments arranged by size:
3986 209 181
BssSI C'ACGA_G
Cuts at: 148 2647 3005 148
Size: 2499 358 1519
Fragments arranged by size:
2499 1519 358
BstAPI GCAn_nnn'nTGC
Cuts at: 486 917 1160 1295 1753 486
Size: 431 243 135 458 3109
Fragments arranged by size:
3109 458 431 243 135
BstBI TT'CG_AA
Cuts at: 203 2737 203
Size: 2534 1842
BstEII G'GTnAC_C
Cuts at: 844 844
Size: 4376
BstXI CCAn_nnnn'nTGG
Cuts at: 114 114
Size: 4376
BstYI r'GATC_y
Cuts at: 1 1401 1868 1891 2226 2472 3484 3570 3582
Size: 1400 467 23 335 246 1012 86 12
Cuts at: 3582 3928 1
Size: 346 449
Fragments arranged by size:
1400 1012 467 449 346 335 246 86
23 12
BstZ17I GTA'TAC
Cuts at: 323 1046 323
Size: 723 3653
BtgI C'CryG_G
Cuts at: 107 770 107
Size: 663 3713
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2326 2507 2670 2326
Size: 181 163 4032
Fragments arranged by size:
4032 181 163
BtsI GCAGTG_nn'
Cuts at: 767 1619 767
Size: 852 3524
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 315 350 315
Size: 35 4341
DraI TTT'AAA
Cuts at: 176 1375 176
Size: 1199 3177
DraIII CAC_nnn'GTG
Cuts at: 1182 1268 1182
Size: 86 4290
DrdI GACnn_nn'nnGTC
Cuts at: 2082 2940 2082
Size: 858 3518
EaeI y'GGCC_r
Cuts at: 246 473 1961 2135 2526 2553 3898 246
Size: 227 1488 174 391 27 1345 724
Fragments arranged by size:
1488 1345 724 391 227 174 27
EagI C'GGCC_G
Cuts at: 1961 1961
Size: 4376
EarI CTCTTCn'nnn_
Cuts at: 248 275 1354 2399 2609 248
Size: 27 1079 1045 210 2015
Fragments arranged by size:
2015 1079 1045 210 27
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2584 2894 3040 4186 2584
Size: 310 146 1146 2774
Fragments arranged by size:
2774 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1388 1388
Size: 4376
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 237 755 2201 2633 3380 3648 237
Size: 518 1446 432 747 268 965
Fragments arranged by size:
1446 965 747 518 432 268
EcoO109I rG'GnC_Cy
Cuts at: 585 712 1515 585
Size: 127 803 3446
Fragments arranged by size:
3446 803 127
EcoRI G'AATT_C
Cuts at: 356 1380 356
Size: 1024 3352
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 156 2395 156
Size: 2239 2137
FspI TGC'GCA
Cuts at: 2157 2157
Size: 4376
HaeII r_GCGC'y
Cuts at: 1810 2058 3080 4059 1810
Size: 248 1022 979 2127
Fragments arranged by size:
2127 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 766 798 2218 2248 2250 2280 766
Size: 32 1420 30 2 30 2862
Fragments arranged by size:
2862 1420 32 30 30 2
HincII GTy'rAC
Cuts at: 936 1415 936
Size: 479 3897
KasI G'GCGC_C
Cuts at: 1806 2054 1806
Size: 248 4128
KpnI G_GTAC'C
Cuts at: 1396 1396
Size: 4376
MluI A'CGCG_T
Cuts at: 3809 3809
Size: 4376
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3047 3231 3047
Size: 184 4192
MscI TGG'CCA
Cuts at: 248 475 2137 248
Size: 227 1662 2487
Fragments arranged by size:
2487 1662 227
MslI CAynn'nnrTG
Cuts at: 490 986 2365 2492 490
Size: 496 1379 127 2374
Fragments arranged by size:
2374 1379 496 127
MspA1I CmG'CkG
Cuts at: 555 1250 1487 1801 2161 3174 3419 555
Size: 695 237 314 360 1013 245 1512
Fragments arranged by size:
1512 1013 695 360 314 245 237
NaeI GCC'GGC
Cuts at: 2557 2557
Size: 4376
NarI GG'CG_CC
Cuts at: 1807 2055 1807
Size: 248 4128
NcoI C'CATG_G
Cuts at: 107 770 107
Size: 663 3713
NgoMIV G'CCGG_C
Cuts at: 2555 2555
Size: 4376
NsiI A_TGCA'T
Cuts at: 1260 1260
Size: 4376
NspI r_CATG'y
Cuts at: 489 1258 1429 1929 2836 489
Size: 769 171 500 907 2029
Fragments arranged by size:
2029 907 769 500 171
PciI A'CATG_T
Cuts at: 2832 2832
Size: 4376
PfoI T'CCnGG_A
Cuts at: 732 732
Size: 4376
PmeI GTTT'AAAC
Cuts at: 1375 1375
Size: 4376
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3515 3547 3515
Size: 32 4344
PpuMI rG'GwC_Cy
Cuts at: 585 585
Size: 4376
PstI C_TGCA'G
Cuts at: 913 1291 1423 2108 913
Size: 378 132 685 3181
Fragments arranged by size:
3181 685 378 132
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4376
PvuII CAG'CTG
Cuts at: 555 1250 1801 2161 555
Size: 695 551 360 2770
Fragments arranged by size:
2770 695 551 360
RsrII CG'GwC_CG
Cuts at: 2571 2571
Size: 4376
SacI G_AGCT'C
Cuts at: 1390 1390
Size: 4376
SalI G'TCGA_C
Cuts at: 1413 1413
Size: 4376
SapI GCTCTTCn'nnn_
Cuts at: 248 2399 2609 248
Size: 2151 210 2015
Fragments arranged by size:
2151 2015 210
SbfI CC_TGCA'GG
Cuts at: 1423 1423
Size: 4376
ScaI AGT'ACT
Cuts at: 3893 3893
Size: 4376
SfcI C'TryA_G
Cuts at: 33 909 1287 1419 2104 3097 3288 33
Size: 876 378 132 685 993 191 1121
Fragments arranged by size:
1121 993 876 685 378 191 132
SfoI GGC'GCC
Cuts at: 1808 2056 1808
Size: 248 4128
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4376
SmaI CCC'GGG
Cuts at: 1398 1398
Size: 4376
SmlI C'TyrA_G
Cuts at: 270 1531 2446 2938 3200 270
Size: 1261 915 492 262 1446
Fragments arranged by size:
1446 1261 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4376
SphI G_CATG'C
Cuts at: 1258 1429 1929 1258
Size: 171 500 3705
Fragments arranged by size:
3705 500 171
SspI AAT'ATT
Cuts at: 808 808
Size: 4376
StyI C'CwwG_G
Cuts at: 107 500 708 770 1510 1864 107
Size: 393 208 62 740 354 2619
Fragments arranged by size:
2619 740 393 354 208 62
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 908 1959 2092 2551 2760 908
Size: 1051 133 459 209 2524
Fragments arranged by size:
2524 1051 459 209 133
TatI w'GTAC_w
Cuts at: 482 3891 482
Size: 3409 967
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3893 3925 3893
Size: 32 4344
Tth111I GACn'n_nGTC
Cuts at: 2173 2173
Size: 4376
XbaI T'CTAG_A
Cuts at: 1407 1407
Size: 4376
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1871 1871
Size: 4376
XmaI C'CCGG_G
Cuts at: 1396 1396
Size: 4376
XmnI GAAnn'nnTTC
Cuts at: 1081 1081
Size: 4376
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
BtgI BtgZI BtsI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NsiI
NspI PciI PfoI PmeI PpiI PpuMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BmgBI BmtI BsaXI BsiWI
BsmBI BsrGI BssHII Bsu36I ClaI EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
PacI PasI PflMI PmlI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
StuI SwaI XhoI ZraI