Restriction map for
pF1KB3474
(Circular) (Six-base) MAPSORT of: pF1KB3474.seq Check: 3706 from: 1 to: 3704
pF1KB3474 3704 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AccI GT'mk_AC
Cuts at: 742 742
Size: 3704
Acc65I G'GTAC_C
Cuts at: 720 720
Size: 3704
AclI AA'CG_TT
Cuts at: 3544 3544
Size: 3704
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1529 1961 2708 2976 1529
Size: 432 747 268 2257
Fragments arranged by size:
2257 747 432 268
AflIII A'CryG_T
Cuts at: 1056 2160 3137 1056
Size: 1104 977 1623
Fragments arranged by size:
1623 1104 977
AgeI A'CCGG_T
Cuts at: 2092 2092
Size: 3704
AleI CACnn'nnGTG
Cuts at: 295 295
Size: 3704
AlwNI CAG_nnn'CTG
Cuts at: 1009 2576 1009
Size: 1567 2137
ApaLI G'TGCA_C
Cuts at: 2474 2474
Size: 3704
ApoI r'AATT_y
Cuts at: 708 3057 3313 708
Size: 2349 256 1099
Fragments arranged by size:
2349 1099 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3704
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3704
AvaI C'yCGr_G
Cuts at: 395 405 724 395
Size: 10 319 3375
Fragments arranged by size:
3375 319 10
BamHI G'GATC_C
Cuts at: 541 729 541
Size: 188 3516
BanI G'GyrC_C
Cuts at: 720 1134 1382 1417 720
Size: 414 248 35 3007
Fragments arranged by size:
3007 414 248 35
BanII G_rGCy'C
Cuts at: 458 676 691 718 1748 458
Size: 218 15 27 1030 2414
Fragments arranged by size:
2414 1030 218 27 15
BbeI G_GCGC'C
Cuts at: 1138 1386 1138
Size: 248 3456
BbsI GAAGACnn'nnnn_
Cuts at: 479 479
Size: 3704
BciVI GTATCCnnnnn_n'
Cuts at: 1602 2363 3704 1602
Size: 761 1341 1602
Fragments arranged by size:
1602 1341 761
BclI T'GATC_A
Cuts at: 1224 1224
Size: 3704
BglI GCCn_nnn'nGGC
Cuts at: 148 148
Size: 3704
BglII A'GATC_T
Cuts at: 1219 3256 1219
Size: 2037 1667
BlpI GC'TnA_GC
Cuts at: 816 816
Size: 3704
Bme1580I G_kGCm'C
Cuts at: 1329 1422 2478 1329
Size: 93 1056 2555
Fragments arranged by size:
2555 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 916 997 1332 3476 916
Size: 81 335 2144 1144
Fragments arranged by size:
2144 1144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2071 2071
Size: 3704
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 442 442
Size: 3704
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 880 1759 2251 2549 880
Size: 879 492 298 2035
Fragments arranged by size:
2035 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 516 3408 516
Size: 2892 812
BsaAI yAC'GTr
Cuts at: 1057 1057
Size: 3704
BsaBI GATnn'nnATC
Cuts at: 6 1218 3255 6
Size: 1212 2037 455
Fragments arranged by size:
2037 1212 455
BsaHI Gr'CG_yC
Cuts at: 511 1135 1383 2085 3581 511
Size: 624 248 702 1496 634
Fragments arranged by size:
1496 702 634 624 248
BsaWI w'CCGG_w
Cuts at: 893 1414 2092 2366 2513 2924 893
Size: 521 678 274 147 411 1673
Fragments arranged by size:
1673 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 191 270 709 191
Size: 79 439 3186
Fragments arranged by size:
3186 439 79
BseYI C'CCAG_C
Cuts at: 351 1129 1887 2464 2960 351
Size: 778 758 577 496 1095
Fragments arranged by size:
1095 778 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 500 500
Size: 3704
BsiEI CG_ry'CG
Cuts at: 104 1246 1292 2500 104
Size: 1142 46 1208 1308
Fragments arranged by size:
1308 1208 1142 46
BsiHKAI G_wGCw'C
Cuts at: 458 691 718 1496 1686 2478 458
Size: 233 27 778 190 792 1684
Fragments arranged by size:
1684 792 778 233 190 27
BsmI GAATG_Cn'
Cuts at: 336 336
Size: 3704
Bsp1286I G_dGCh'C
Cuts at: 458 676 691 718 1329 1422 1496 1686 1748
Size: 218 15 27 611 93 74 190 62
Cuts at: 1748 2478 458
Size: 730 1684
Fragments arranged by size:
1684 730 611 218 190 93 74 62
27 15
BspEI T'CCGG_A
Cuts at: 2924 2924
Size: 3704
BspHI T'CATG_A
Cuts at: 2880 2880
Size: 3704
BspMI ACCTGCnnnn'nnnn_
Cuts at: 754 968 1270 1651 754
Size: 214 302 381 2807
Fragments arranged by size:
2807 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1996 2050 3534 3699 50
Size: 1946 54 1484 165 55
Fragments arranged by size:
1946 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1616 1616
Size: 3704
BsrFI r'CCGG_y
Cuts at: 1702 1883 2092 1702
Size: 181 209 3314
Fragments arranged by size:
3314 209 181
BssSI C'ACGA_G
Cuts at: 1975 2333 1975
Size: 358 3346
BstAPI GCAn_nnn'nTGC
Cuts at: 1081 1081
Size: 3704
BstBI TT'CG_AA
Cuts at: 2065 2065
Size: 3704
BstXI CCAn_nnnn'nTGG
Cuts at: 271 552 271
Size: 281 3423
BstYI r'GATC_y
Cuts at: 1 541 729 1196 1219 1554 1800 2812 2898
Size: 540 188 467 23 335 246 1012 86
Cuts at: 2898 2910 3256 1
Size: 12 346 449
Fragments arranged by size:
1012 540 467 449 346 335 246 188
86 23 12
BtgI C'CryG_G
Cuts at: 107 569 107
Size: 462 3242
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1654 1835 1998 1654
Size: 181 163 3360
Fragments arranged by size:
3360 181 163
BtsI GCAGTG_nn'
Cuts at: 947 947
Size: 3704
DraI TTT'AAA
Cuts at: 703 703
Size: 3704
DrdI GACnn_nn'nnGTC
Cuts at: 1410 2268 1410
Size: 858 2846
EaeI y'GGCC_r
Cuts at: 554 1289 1463 1854 1881 3226 554
Size: 735 174 391 27 1345 1032
Fragments arranged by size:
1345 1032 735 391 174 27
EagI C'GGCC_G
Cuts at: 1289 1289
Size: 3704
EarI CTCTTCn'nnn_
Cuts at: 514 1727 1937 514
Size: 1213 210 2281
Fragments arranged by size:
2281 1213 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 373 1912 2222 2368 3514 373
Size: 1539 310 146 1146 563
Fragments arranged by size:
1539 1146 563 310 146
EcoICRI GAG'CTC
Cuts at: 456 689 716 456
Size: 233 27 3444
Fragments arranged by size:
3444 233 27
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 442 1529 1961 2708 2976 442
Size: 1087 432 747 268 1170
Fragments arranged by size:
1170 1087 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 531 531
Size: 3704
EcoO109I rG'GnC_Cy
Cuts at: 275 437 843 275
Size: 162 406 3136
Fragments arranged by size:
3136 406 162
EcoRI G'AATT_C
Cuts at: 708 708
Size: 3704
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1723 1723
Size: 3704
FspI TGC'GCA
Cuts at: 1485 1485
Size: 3704
HaeII r_GCGC'y
Cuts at: 639 1138 1386 2408 3387 639
Size: 499 248 1022 979 956
Fragments arranged by size:
1022 979 956 499 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 267 299 424 456 1546 1576 1578 1608 267
Size: 32 125 32 1090 30 2 30 2363
Fragments arranged by size:
2363 1090 125 32 32 30 30 2
HincII GTy'rAC
Cuts at: 743 743
Size: 3704
KasI G'GCGC_C
Cuts at: 1134 1382 1134
Size: 248 3456
KpnI G_GTAC'C
Cuts at: 724 724
Size: 3704
MluI A'CGCG_T
Cuts at: 3137 3137
Size: 3704
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 473 2375 2559 473
Size: 1902 184 1618
Fragments arranged by size:
1902 1618 184
MscI TGG'CCA
Cuts at: 556 1465 556
Size: 909 2795
MslI CAynn'nnrTG
Cuts at: 295 1693 1820 295
Size: 1398 127 2179
Fragments arranged by size:
2179 1398 127
MspA1I CmG'CkG
Cuts at: 295 319 355 815 1129 1489 2502 2747 295
Size: 24 36 460 314 360 1013 245 1252
Fragments arranged by size:
1252 1013 460 360 314 245 36 24
NaeI GCC'GGC
Cuts at: 1885 1885
Size: 3704
NarI GG'CG_CC
Cuts at: 1135 1383 1135
Size: 248 3456
NcoI C'CATG_G
Cuts at: 107 569 107
Size: 462 3242
NgoMIV G'CCGG_C
Cuts at: 1883 1883
Size: 3704
NspI r_CATG'y
Cuts at: 757 1257 2164 757
Size: 500 907 2297
Fragments arranged by size:
2297 907 500
PasI CC'CwG_GG
Cuts at: 361 361
Size: 3704
PciI A'CATG_T
Cuts at: 2160 2160
Size: 3704
PmeI GTTT'AAAC
Cuts at: 703 703
Size: 3704
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2843 2875 2843
Size: 32 3672
PpuMI rG'GwC_Cy
Cuts at: 275 275
Size: 3704
PstI C_TGCA'G
Cuts at: 751 1436 751
Size: 685 3019
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3704
PvuII CAG'CTG
Cuts at: 355 1129 1489 355
Size: 774 360 2570
Fragments arranged by size:
2570 774 360
RsrII CG'GwC_CG
Cuts at: 1899 1899
Size: 3704
SacI G_AGCT'C
Cuts at: 458 691 718 458
Size: 233 27 3444
Fragments arranged by size:
3444 233 27
SalI G'TCGA_C
Cuts at: 741 741
Size: 3704
SapI GCTCTTCn'nnn_
Cuts at: 1727 1937 1727
Size: 210 3494
SbfI CC_TGCA'GG
Cuts at: 751 751
Size: 3704
ScaI AGT'ACT
Cuts at: 3221 3221
Size: 3704
SfcI C'TryA_G
Cuts at: 33 747 1432 2425 2616 33
Size: 714 685 993 191 1121
Fragments arranged by size:
1121 993 714 685 191
SfoI GGC'GCC
Cuts at: 1136 1384 1136
Size: 248 3456
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3704
SmaI CCC'GGG
Cuts at: 407 726 407
Size: 319 3385
SmlI C'TyrA_G
Cuts at: 859 1774 2266 2528 859
Size: 915 492 262 2035
Fragments arranged by size:
2035 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3704
SphI G_CATG'C
Cuts at: 757 1257 757
Size: 500 3204
StyI C'CwwG_G
Cuts at: 107 569 598 838 1192 107
Size: 462 29 240 354 2619
Fragments arranged by size:
2619 462 354 240 29
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 193 365 1287 1420 1879 2088 193
Size: 172 922 133 459 209 1809
Fragments arranged by size:
1809 922 459 209 172 133
TatI w'GTAC_w
Cuts at: 3219 3219
Size: 3704
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3221 3253 3221
Size: 32 3672
Tth111I GACn'n_nGTC
Cuts at: 1501 1501
Size: 3704
XbaI T'CTAG_A
Cuts at: 735 735
Size: 3704
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 583 1199 583
Size: 616 3088
XmaI C'CCGG_G
Cuts at: 405 724 405
Size: 319 3385
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PmeI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI
Bpu10I BsaXI BsiWI BsmBI BsrGI BssHII BstEII BstZ17I
Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PflMI PfoI PmlI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI