Restriction map for
pF1KB3464
(Circular) (Six-base) MAPSORT of: pF1KB3464.seq Check: 222 from: 1 to: 3977
pF1KB3464 3977 bp
With 182 enzymes: SgfI *
April 24, 2009 13:01 ..
AccI GT'mk_AC
Cuts at: 634 1015 634
Size: 381 3596
Acc65I G'GTAC_C
Cuts at: 993 993
Size: 3977
AclI AA'CG_TT
Cuts at: 3817 3817
Size: 3977
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 835 1802 2234 2981 3249 835
Size: 967 432 747 268 1563
Fragments arranged by size:
1563 967 747 432 268
AflIII A'CryG_T
Cuts at: 1329 2433 3410 1329
Size: 1104 977 1896
Fragments arranged by size:
1896 1104 977
AgeI A'CCGG_T
Cuts at: 2365 2365
Size: 3977
AlwNI CAG_nnn'CTG
Cuts at: 873 1282 2849 873
Size: 409 1567 2001
Fragments arranged by size:
2001 1567 409
ApaLI G'TGCA_C
Cuts at: 2747 2747
Size: 3977
ApoI r'AATT_y
Cuts at: 981 3330 3586 981
Size: 2349 256 1372
Fragments arranged by size:
2349 1372 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3977
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3977
AvaI C'yCGr_G
Cuts at: 997 997
Size: 3977
BamHI G'GATC_C
Cuts at: 1002 1002
Size: 3977
BanI G'GyrC_C
Cuts at: 574 993 1407 1655 1690 574
Size: 419 414 248 35 2861
Fragments arranged by size:
2861 419 414 248 35
BanII G_rGCy'C
Cuts at: 807 991 2021 807
Size: 184 1030 2763
Fragments arranged by size:
2763 1030 184
BbeI G_GCGC'C
Cuts at: 1411 1659 1411
Size: 248 3729
BbsI GAAGACnn'nnnn_
Cuts at: 903 903
Size: 3977
BciVI GTATCCnnnnn_n'
Cuts at: 1875 2636 3977 1875
Size: 761 1341 1875
Fragments arranged by size:
1875 1341 761
BclI T'GATC_A
Cuts at: 1497 1497
Size: 3977
BfrBI ATG'CAT
Cuts at: 796 796
Size: 3977
BglI GCCn_nnn'nGGC
Cuts at: 968 968
Size: 3977
BglII A'GATC_T
Cuts at: 1492 3529 1492
Size: 2037 1940
BlpI GC'TnA_GC
Cuts at: 1089 1089
Size: 3977
Bme1580I G_kGCm'C
Cuts at: 577 1602 1695 2751 577
Size: 1025 93 1056 1803
Fragments arranged by size:
1803 1056 1025 93
BmrI ACTGGGnnnn_n'
Cuts at: 170 1189 1270 1605 3749 170
Size: 1019 81 335 2144 398
Fragments arranged by size:
2144 1019 398 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 915 2344 915
Size: 1429 2548
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 700 700
Size: 3977
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1153 2032 2524 2822 1153
Size: 879 492 298 2308
Fragments arranged by size:
2308 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3681 3681
Size: 3977
BsaAI yAC'GTr
Cuts at: 1330 1330
Size: 3977
BsaBI GATnn'nnATC
Cuts at: 6 1491 3528 6
Size: 1485 2037 455
Fragments arranged by size:
2037 1485 455
BsaHI Gr'CG_yC
Cuts at: 1408 1656 2358 3854 1408
Size: 248 702 1496 1531
Fragments arranged by size:
1531 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1166 1687 2365 2639 2786 3197 1166
Size: 521 678 274 147 411 1946
Fragments arranged by size:
1946 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 822 852 822
Size: 30 3947
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 344 410 344
Size: 66 3911
BseYI C'CCAG_C
Cuts at: 143 1402 2160 2737 3233 143
Size: 1259 758 577 496 887
Fragments arranged by size:
1259 887 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 730 730
Size: 3977
BsiEI CG_ry'CG
Cuts at: 104 1519 1565 2773 104
Size: 1415 46 1208 1308
Fragments arranged by size:
1415 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 991 1769 1959 2751 991
Size: 778 190 792 2217
Fragments arranged by size:
2217 792 778 190
BsmI GAATG_Cn'
Cuts at: 495 798 495
Size: 303 3674
Bsp1286I G_dGCh'C
Cuts at: 577 807 991 1602 1695 1769 1959 2021 2751
Size: 230 184 611 93 74 190 62 730
Cuts at: 2751 577
Size: 1803
Fragments arranged by size:
1803 730 611 230 190 184 93 74
62
BspEI T'CCGG_A
Cuts at: 3197 3197
Size: 3977
BspHI T'CATG_A
Cuts at: 3153 3153
Size: 3977
BspMI ACCTGCnnnn'nnnn_
Cuts at: 872 1027 1241 1543 1924 872
Size: 155 214 302 381 2925
Fragments arranged by size:
2925 381 302 214 155
BsrBI CCG'CTC
Cuts at: 50 2269 2323 3807 3972 50
Size: 2219 54 1484 165 55
Fragments arranged by size:
2219 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 881 1889 881
Size: 1008 2969
BsrFI r'CCGG_y
Cuts at: 1975 2156 2365 1975
Size: 181 209 3587
Fragments arranged by size:
3587 209 181
BssSI C'ACGA_G
Cuts at: 2248 2606 2248
Size: 358 3619
BstAPI GCAn_nnn'nTGC
Cuts at: 1354 1354
Size: 3977
BstBI TT'CG_AA
Cuts at: 2338 2338
Size: 3977
BstYI r'GATC_y
Cuts at: 1 428 593 1002 1469 1492 1827 2073 3085
Size: 427 165 409 467 23 335 246 1012
Cuts at: 3085 3171 3183 3529 1
Size: 86 12 346 449
Fragments arranged by size:
1012 467 449 427 409 346 335 246
165 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 422 422
Size: 3977
BtgI C'CryG_G
Cuts at: 107 821 107
Size: 714 3263
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1927 2108 2271 1927
Size: 181 163 3633
Fragments arranged by size:
3633 181 163
BtsI GCAGTG_nn'
Cuts at: 1220 1220
Size: 3977
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 440 475 440
Size: 35 3942
DraI TTT'AAA
Cuts at: 976 976
Size: 3977
DrdI GACnn_nn'nnGTC
Cuts at: 1683 2541 1683
Size: 858 3119
EaeI y'GGCC_r
Cuts at: 110 1562 1736 2127 2154 3499 110
Size: 1452 174 391 27 1345 588
Fragments arranged by size:
1452 1345 588 391 174 27
EagI C'GGCC_G
Cuts at: 1562 1562
Size: 3977
EarI CTCTTCn'nnn_
Cuts at: 2000 2210 2000
Size: 210 3767
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2185 2495 2641 3787 2185
Size: 310 146 1146 2375
Fragments arranged by size:
2375 1146 310 146
EcoICRI GAG'CTC
Cuts at: 989 989
Size: 3977
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 700 835 1802 2234 2981 3249 700
Size: 135 967 432 747 268 1428
Fragments arranged by size:
1428 967 747 432 268 135
EcoNI CCTnn'n_nnAGG
Cuts at: 436 436
Size: 3977
EcoO109I rG'GnC_Cy
Cuts at: 183 340 1116 183
Size: 157 776 3044
Fragments arranged by size:
3044 776 157
EcoRI G'AATT_C
Cuts at: 981 981
Size: 3977
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1996 1996
Size: 3977
FspI TGC'GCA
Cuts at: 1758 1758
Size: 3977
HaeII r_GCGC'y
Cuts at: 1411 1659 2681 3660 1411
Size: 248 1022 979 1728
Fragments arranged by size:
1728 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 284 316 1819 1849 1851 1881 284
Size: 32 1503 30 2 30 2380
Fragments arranged by size:
2380 1503 32 30 30 2
HincII GTy'rAC
Cuts at: 1016 1016
Size: 3977
KasI G'GCGC_C
Cuts at: 1407 1655 1407
Size: 248 3729
KpnI G_GTAC'C
Cuts at: 997 997
Size: 3977
MfeI C'AATT_G
Cuts at: 905 905
Size: 3977
MluI A'CGCG_T
Cuts at: 3410 3410
Size: 3977
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2648 2832 2648
Size: 184 3793
MscI TGG'CCA
Cuts at: 1738 1738
Size: 3977
MslI CAynn'nnrTG
Cuts at: 385 928 1966 2093 385
Size: 543 1038 127 2269
Fragments arranged by size:
2269 1038 543 127
MspA1I CmG'CkG
Cuts at: 147 1088 1402 1762 2775 3020 147
Size: 941 314 360 1013 245 1104
Fragments arranged by size:
1104 1013 941 360 314 245
NaeI GCC'GGC
Cuts at: 2158 2158
Size: 3977
NarI GG'CG_CC
Cuts at: 1408 1656 1408
Size: 248 3729
NcoI C'CATG_G
Cuts at: 107 821 107
Size: 714 3263
NgoMIV G'CCGG_C
Cuts at: 2156 2156
Size: 3977
NsiI A_TGCA'T
Cuts at: 798 798
Size: 3977
NspI r_CATG'y
Cuts at: 630 1030 1530 2437 630
Size: 400 500 907 2170
Fragments arranged by size:
2170 907 500 400
PciI A'CATG_T
Cuts at: 2433 2433
Size: 3977
PmeI GTTT'AAAC
Cuts at: 976 976
Size: 3977
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3116 3148 3116
Size: 32 3945
PpuMI rG'GwC_Cy
Cuts at: 340 340
Size: 3977
PsiI TTA'TAA
Cuts at: 238 277 238
Size: 39 3938
PstI C_TGCA'G
Cuts at: 735 846 869 1024 1709 735
Size: 111 23 155 685 3003
Fragments arranged by size:
3003 685 155 111 23
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3977
PvuII CAG'CTG
Cuts at: 1402 1762 1402
Size: 360 3617
RsrII CG'GwC_CG
Cuts at: 2172 2172
Size: 3977
SacI G_AGCT'C
Cuts at: 991 991
Size: 3977
SalI G'TCGA_C
Cuts at: 1014 1014
Size: 3977
SapI GCTCTTCn'nnn_
Cuts at: 2000 2210 2000
Size: 210 3767
SbfI CC_TGCA'GG
Cuts at: 1024 1024
Size: 3977
ScaI AGT'ACT
Cuts at: 3494 3494
Size: 3977
SexAI A'CCwGG_T
Cuts at: 342 342
Size: 3977
SfcI C'TryA_G
Cuts at: 33 731 842 865 1020 1705 2698 2889 33
Size: 698 111 23 155 685 993 191 1121
Fragments arranged by size:
1121 993 698 685 191 155 111 23
SfoI GGC'GCC
Cuts at: 1409 1657 1409
Size: 248 3729
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3977
SmaI CCC'GGG
Cuts at: 999 999
Size: 3977
SmlI C'TyrA_G
Cuts at: 1132 2047 2539 2801 1132
Size: 915 492 262 2308
Fragments arranged by size:
2308 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3977
SphI G_CATG'C
Cuts at: 1030 1530 1030
Size: 500 3477
StyI C'CwwG_G
Cuts at: 107 186 764 821 1111 1465 107
Size: 79 578 57 290 354 2619
Fragments arranged by size:
2619 578 354 290 79 57
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1560 1693 2152 2361 1560
Size: 133 459 209 3176
Fragments arranged by size:
3176 459 209 133
TatI w'GTAC_w
Cuts at: 3492 3492
Size: 3977
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 656 688 822 854 3494 3526 656
Size: 32 134 32 2640 32 1107
Fragments arranged by size:
2640 1107 134 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1774 1774
Size: 3977
XbaI T'CTAG_A
Cuts at: 1008 1008
Size: 3977
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 254 1472 254
Size: 1218 2759
XmaI C'CCGG_G
Cuts at: 997 997
Size: 3977
XmnI GAAnn'nnTTC
Cuts at: 402 402
Size: 3977
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NsiI NspI PciI PmeI
PpiI PpuMI PsiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BmgBI BmtI
Bpu10I BsiWI BsmBI BsrGI BssHII BstEII BstXI BstZ17I
ClaI DraIII EcoRV FseI FspAI HindIII HpaI NdeI
NheI NotI NruI PacI PasI PflMI PfoI PmlI
PshAI PspOMI PspXI PsrI SacII SanDI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI ZraI