Restriction map for
pF1KB3458
(Circular) (Six-base) MAPSORT of: pF1KB3458.seq Check: 1653 from: 1 to: 6464
pF1KB3458 6464 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 705 1151 1955 705
Size: 446 804 5214
Fragments arranged by size:
5214 804 446
AatII G_ACGT'C
Cuts at: 3356 3356
Size: 6464
AccI GT'mk_AC
Cuts at: 1180 3502 1180
Size: 2322 4142
Acc65I G'GTAC_C
Cuts at: 3480 3480
Size: 6464
AclI AA'CG_TT
Cuts at: 6304 6304
Size: 6464
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 754 1348 1681 2013 3410 3424 4289 4721 5468
Size: 594 333 332 1397 14 865 432 747
Cuts at: 5468 5736 754
Size: 268 1482
Fragments arranged by size:
1482 1397 865 747 594 432 333 332
268 14
AflIII A'CryG_T
Cuts at: 2645 3816 4920 5897 2645
Size: 1171 1104 977 3212
Fragments arranged by size:
3212 1171 1104 977
AgeI A'CCGG_T
Cuts at: 4852 4852
Size: 6464
AleI CACnn'nnGTG
Cuts at: 550 550
Size: 6464
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1643 1643
Size: 6464
AlwNI CAG_nnn'CTG
Cuts at: 123 379 603 660 825 858 876 1518 2167
Size: 256 224 57 165 33 18 642 649
Cuts at: 2167 2874 2946 3769 5336 123
Size: 707 72 823 1567 1251
Fragments arranged by size:
1567 1251 823 707 649 642 256 224
165 72 57 33 18
ApaI G_GGCC'C
Cuts at: 3272 3272
Size: 6464
ApaLI G'TGCA_C
Cuts at: 5234 5234
Size: 6464
ApoI r'AATT_y
Cuts at: 575 989 2349 2395 3468 5817 6073 575
Size: 414 1360 46 1073 2349 256 966
Fragments arranged by size:
2349 1360 1073 966 414 256 46
AseI AT'TA_AT
Cuts at: 20 20
Size: 6464
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6464
AvaI C'yCGr_G
Cuts at: 345 1433 1890 1936 2019 2996 3484 345
Size: 1088 457 46 83 977 488 3325
Fragments arranged by size:
3325 1088 977 488 457 83 46
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1066 1099 1066
Size: 33 6431
BamHI G'GATC_C
Cuts at: 1481 3489 1481
Size: 2008 4456
BanI G'GyrC_C
Cuts at: 435 717 797 834 1823 1894 2062 2805 2974
Size: 282 80 37 989 71 168 743 169
Cuts at: 2974 3001 3034 3279 3480 3894 4142 4177 435
Size: 27 33 245 201 414 248 35 2722
Fragments arranged by size:
2722 989 743 414 282 248 245 201
169 168 80 71 37 35 33 27
BanII G_rGCy'C
Cuts at: 455 636 683 1225 1253 1372 1423 1569 1790
Size: 181 47 542 28 119 51 146 221
Cuts at: 1790 2005 2020 2027 3067 3272 3478 4508 455
Size: 215 15 7 1040 205 206 1030 2411
Fragments arranged by size:
2411 1040 1030 542 221 215 206 205
181 146 119 51 47 28 15 7
BbeI G_GCGC'C
Cuts at: 1898 2066 3283 3898 4146 1898
Size: 168 1217 615 248 4216
Fragments arranged by size:
4216 1217 615 248 168
BbsI GAAGACnn'nnnn_
Cuts at: 1336 3132 1336
Size: 1796 4668
BbvCI CC'TCA_GC
Cuts at: 1505 1505
Size: 6464
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 973 1007 973
Size: 34 6430
BciVI GTATCCnnnnn_n'
Cuts at: 333 4362 5123 6464 333
Size: 4029 761 1341 333
Fragments arranged by size:
4029 1341 761 333
BclI T'GATC_A
Cuts at: 3314 3420 3984 3314
Size: 106 564 5794
Fragments arranged by size:
5794 564 106
BglI GCCn_nnn'nGGC
Cuts at: 253 253
Size: 6464
BglII A'GATC_T
Cuts at: 1425 3979 6016 1425
Size: 2554 2037 1873
Fragments arranged by size:
2554 2037 1873
BlpI GC'TnA_GC
Cuts at: 449 1366 1999 3576 449
Size: 917 633 1577 3337
Fragments arranged by size:
3337 1577 917 633
Bme1580I G_kGCm'C
Cuts at: 438 722 800 1775 2977 3272 4089 4182 5238
Size: 284 78 975 1202 295 817 93 1056
Cuts at: 5238 438
Size: 1664
Fragments arranged by size:
1664 1202 1056 975 817 295 284 93
78
BmrI ACTGGGnnnn_n'
Cuts at: 1227 1469 1520 3676 3757 4092 6236 1227
Size: 242 51 2156 81 335 2144 1455
Fragments arranged by size:
2156 2144 1455 335 242 81 51
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4831 4831
Size: 6464
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2189 2308 2189
Size: 119 6345
Bpu10I CC'TnA_GC
Cuts at: 830 1505 830
Size: 675 5789
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 266 1777 2232 3640 4519 5011 5309 266
Size: 1511 455 1408 879 492 298 1421
Fragments arranged by size:
1511 1421 1408 879 492 455 298
BsaI GGTCTCn'nnnn_
Cuts at: 1403 6168 1403
Size: 4765 1699
BsaAI yAC'GTr
Cuts at: 400 2648 3817 400
Size: 2248 1169 3047
Fragments arranged by size:
3047 2248 1169
BsaBI GATnn'nnATC
Cuts at: 6 3978 6015 6
Size: 3972 2037 455
Fragments arranged by size:
3972 2037 455
BsaHI Gr'CG_yC
Cuts at: 188 1895 2063 2086 2095 3280 3353 3895 4143
Size: 1707 168 23 9 1185 73 542 248
Cuts at: 4143 4845 6341 188
Size: 702 1496 311
Fragments arranged by size:
1707 1496 1185 702 542 311 248 168
73 23 9
BsaWI w'CCGG_w
Cuts at: 497 2681 3653 4174 4852 5126 5273 5684 497
Size: 2184 972 521 678 274 147 411 1277
Fragments arranged by size:
2184 1277 972 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 463 493 463
Size: 30 6434
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1798 2758 2761 2764 2767 2770 1798
Size: 960 3 3 3 3 5492
Fragments arranged by size:
5492 960 3 3 3 3
BseYI C'CCAG_C
Cuts at: 684 722 1253 1298 1413 1568 2667 3889 4647
Size: 38 531 45 115 155 1099 1222 758
Cuts at: 4647 5224 5720 684
Size: 577 496 1428
Fragments arranged by size:
1428 1222 1099 758 577 531 496 155
115 45 38
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 589 862 1822 589
Size: 273 960 5231
Fragments arranged by size:
5231 960 273
BsiEI CG_ry'CG
Cuts at: 104 1831 4006 4052 5260 104
Size: 1727 2175 46 1208 1308
Fragments arranged by size:
2175 1727 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 648 1372 1423 3067 3478 4256 4446 5238 648
Size: 724 51 1644 411 778 190 792 1874
Fragments arranged by size:
1874 1644 792 778 724 411 190 51
BsmI GAATG_Cn'
Cuts at: 225 225
Size: 6464
Bsp1286I G_dGCh'C
Cuts at: 438 455 636 648 683 722 800 1225 1253
Size: 17 181 12 35 39 78 425 28
Cuts at: 1253 1372 1423 1569 1775 1790 2005 2020 2027
Size: 119 51 146 206 15 215 15 7
Cuts at: 2027 2977 3067 3272 3478 4089 4182 4256 4446
Size: 950 90 205 206 611 93 74 190
Cuts at: 4446 4508 5238 438
Size: 62 730 1664
Fragments arranged by size:
1664 950 730 611 425 215 206 206
205 190 181 146 119 93 90 78
74 62 51 39 35 28 17 15
15 12 7
BspEI T'CCGG_A
Cuts at: 497 5684 497
Size: 5187 1277
BspHI T'CATG_A
Cuts at: 5640 5640
Size: 6464
BspMI ACCTGCnnnn'nnnn_
Cuts at: 578 705 1151 1685 1955 3514 3728 4030 4411
Size: 127 446 534 270 1559 214 302 381
Cuts at: 4411 578
Size: 2631
Fragments arranged by size:
2631 1559 534 446 381 302 270 214
127
BsrBI CCG'CTC
Cuts at: 50 4756 4810 6294 6459 50
Size: 4706 54 1484 165 55
Fragments arranged by size:
4706 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2040 2368 4376 2040
Size: 328 2008 4128
Fragments arranged by size:
4128 2008 328
BsrFI r'CCGG_y
Cuts at: 690 1826 2512 4462 4643 4852 690
Size: 1136 686 1950 181 209 2302
Fragments arranged by size:
2302 1950 1136 686 209 181
BsrGI T'GTAC_A
Cuts at: 125 2592 125
Size: 2467 3997
BssSI C'ACGA_G
Cuts at: 1966 4735 5093 1966
Size: 2769 358 3337
Fragments arranged by size:
3337 2769 358
BstAPI GCAn_nnn'nTGC
Cuts at: 897 1113 1137 3841 897
Size: 216 24 2704 3520
Fragments arranged by size:
3520 2704 216 24
BstBI TT'CG_AA
Cuts at: 4825 4825
Size: 6464
BstEII G'GTnAC_C
Cuts at: 112 2154 2985 112
Size: 2042 831 3591
Fragments arranged by size:
3591 2042 831
BstXI CCAn_nnnn'nTGG
Cuts at: 141 214 400 2059 2619 141
Size: 73 186 1659 560 3986
Fragments arranged by size:
3986 1659 560 186 73
BstYI r'GATC_y
Cuts at: 1 494 1425 1481 3489 3956 3979 4314 4560
Size: 493 931 56 2008 467 23 335 246
Cuts at: 4560 5572 5658 5670 6016 1
Size: 1012 86 12 346 449
Fragments arranged by size:
2008 1012 931 493 467 449 346 335
246 86 56 23 12
BstZ17I GTA'TAC
Cuts at: 1181 1181
Size: 6464
Bsu36I CC'TnA_GG
Cuts at: 251 2886 251
Size: 2635 3829
BtgI C'CryG_G
Cuts at: 107 147 674 1374 1707 2052 2104 2849 2967
Size: 40 527 700 333 345 52 745 118
Cuts at: 2967 3272 107
Size: 305 3299
Fragments arranged by size:
3299 745 700 527 345 333 305 118
52 40
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1441 4414 4595 4758 1441
Size: 2973 181 163 3147
Fragments arranged by size:
3147 2973 181 163
BtsI GCAGTG_nn'
Cuts at: 626 856 3707 626
Size: 230 2851 3383
Fragments arranged by size:
3383 2851 230
ClaI AT'CG_AT
Cuts at: 2135 2135
Size: 6464
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 3111 3146 3111
Size: 35 6429
DraI TTT'AAA
Cuts at: 3463 3463
Size: 6464
DrdI GACnn_nn'nnGTC
Cuts at: 3352 3355 4170 5028 3352
Size: 3 815 858 4788
Fragments arranged by size:
4788 858 815 3
EaeI y'GGCC_r
Cuts at: 330 410 1593 1692 1828 2049 2299 2952 4049
Size: 80 1183 99 136 221 250 653 1097
Cuts at: 4049 4223 4614 4641 5986 330
Size: 174 391 27 1345 808
Fragments arranged by size:
1345 1183 1097 808 653 391 250 221
174 136 99 80 27
EagI C'GGCC_G
Cuts at: 1828 4049 1828
Size: 2221 4243
EarI CTCTTCn'nnn_
Cuts at: 773 1846 2032 2729 2789 3377 4487 4697 773
Size: 1073 186 697 60 588 1110 210 2540
Fragments arranged by size:
2540 1110 1073 697 588 210 186 60
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 251 640 2688 4672 4982 5128 6274 251
Size: 389 2048 1984 310 146 1146 441
Fragments arranged by size:
2048 1984 1146 441 389 310 146
EcoICRI GAG'CTC
Cuts at: 1370 1421 3065 3476 1370
Size: 51 1644 411 4358
Fragments arranged by size:
4358 1644 411 51
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 754 1348 1681 2013 2189 2308 3410 3424 4289
Size: 594 333 332 176 119 1102 14 865
Cuts at: 4289 4721 5468 5736 754
Size: 432 747 268 1482
Fragments arranged by size:
1482 1102 865 747 594 432 333 332
268 176 119 14
EcoNI CCTnn'n_nnAGG
Cuts at: 791 1397 1738 2260 791
Size: 606 341 522 4995
Fragments arranged by size:
4995 606 522 341
EcoO109I rG'GnC_Cy
Cuts at: 376 864 1167 1380 1387 1501 1577 1721 2164
Size: 488 303 213 7 114 76 144 443
Cuts at: 2164 2760 2871 3268 3603 376
Size: 596 111 397 335 3237
Fragments arranged by size:
3237 596 488 443 397 335 303 213
144 114 111 76 7
EcoRI G'AATT_C
Cuts at: 575 3468 575
Size: 2893 3571
EcoRV GAT'ATC
Cuts at: 2247 2247
Size: 6464
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4483 4483
Size: 6464
FspI TGC'GCA
Cuts at: 4245 4245
Size: 6464
HaeII r_GCGC'y
Cuts at: 1898 2066 3283 3898 4146 5168 6147 1898
Size: 168 1217 615 248 1022 979 2215
Fragments arranged by size:
2215 1217 1022 979 615 248 168
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1410 1442 2427 2459 2607 2639 2764 2796 2990
Size: 32 985 32 148 32 125 32 194
Cuts at: 2990 3022 4306 4336 4338 4368 1410
Size: 32 1284 30 2 30 3506
Fragments arranged by size:
3506 1284 985 194 148 125 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 585 3503 585
Size: 2918 3546
HindIII A'AGCT_T
Cuts at: 508 508
Size: 6464
KasI G'GCGC_C
Cuts at: 1894 2062 3279 3894 4142 1894
Size: 168 1217 615 248 4216
Fragments arranged by size:
4216 1217 615 248 168
KpnI G_GTAC'C
Cuts at: 3484 3484
Size: 6464
MluI A'CGCG_T
Cuts at: 5897 5897
Size: 6464
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2150 5135 5319 2150
Size: 2985 184 3295
Fragments arranged by size:
3295 2985 184
MscI TGG'CCA
Cuts at: 332 412 1595 1694 2301 2954 4225 332
Size: 80 1183 99 607 653 1271 2571
Fragments arranged by size:
2571 1271 1183 653 607 99 80
MslI CAynn'nnrTG
Cuts at: 152 212 550 1402 2286 4453 4580 152
Size: 60 338 852 884 2167 127 2036
Fragments arranged by size:
2167 2036 884 852 338 127 60
MspA1I CmG'CkG
Cuts at: 197 518 657 1047 1056 1209 1326 1518 1917
Size: 321 139 390 9 153 117 192 399
Cuts at: 1917 2076 3089 3235 3306 3575 3889 4249 5262
Size: 159 1013 146 71 269 314 360 1013
Cuts at: 5262 5507 197
Size: 245 1154
Fragments arranged by size:
1154 1013 1013 399 390 360 321 314
269 245 192 159 153 146 139 117
71 9
NaeI GCC'GGC
Cuts at: 4645 4645
Size: 6464
NarI GG'CG_CC
Cuts at: 1895 2063 3280 3895 4143 1895
Size: 168 1217 615 248 4216
Fragments arranged by size:
4216 1217 615 248 168
NcoI C'CATG_G
Cuts at: 107 147 674 1374 107
Size: 40 527 700 5197
Fragments arranged by size:
5197 700 527 40
NgoMIV G'CCGG_C
Cuts at: 4643 4643
Size: 6464
NspI r_CATG'y
Cuts at: 891 1649 1701 3517 4017 4924 891
Size: 758 52 1816 500 907 2431
Fragments arranged by size:
2431 1816 907 758 500 52
PasI CC'CwG_GG
Cuts at: 1218 2161 2874 1218
Size: 943 713 4808
Fragments arranged by size:
4808 943 713
PciI A'CATG_T
Cuts at: 4920 4920
Size: 6464
PflMI CCAn_nnn'nTGG
Cuts at: 184 2167 3366 184
Size: 1983 1199 3282
Fragments arranged by size:
3282 1983 1199
PfoI T'CCnGG_A
Cuts at: 1952 1952
Size: 6464
PmeI GTTT'AAAC
Cuts at: 3463 3463
Size: 6464
PmlI CAC'GTG
Cuts at: 2648 2648
Size: 6464
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5603 5635 5603
Size: 32 6432
PpuMI rG'GwC_Cy
Cuts at: 1167 1380 1577 2760 1167
Size: 213 197 1183 4871
Fragments arranged by size:
4871 1183 213 197
PshAI GACnn'nnGTC
Cuts at: 1435 1435
Size: 6464
PspOMI G'GGCC_C
Cuts at: 3268 3268
Size: 6464
PspXI vC'TCGA_Gb
Cuts at: 345 345
Size: 6464
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 151 183 151
Size: 32 6432
PstI C_TGCA'G
Cuts at: 863 1052 1148 2870 3511 4196 863
Size: 189 96 1722 641 685 3131
Fragments arranged by size:
3131 1722 685 641 189 96
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6464
PvuII CAG'CTG
Cuts at: 657 1047 1209 1326 1518 1917 2076 3235 3306
Size: 390 162 117 192 399 159 1159 71
Cuts at: 3306 3889 4249 657
Size: 583 360 2872
Fragments arranged by size:
2872 1159 583 399 390 360 192 162
159 117 71
RsrII CG'GwC_CG
Cuts at: 4659 4659
Size: 6464
SacI G_AGCT'C
Cuts at: 1372 1423 3067 3478 1372
Size: 51 1644 411 4358
Fragments arranged by size:
4358 1644 411 51
SalI G'TCGA_C
Cuts at: 3501 3501
Size: 6464
SanDI GG'GwC_CC
Cuts at: 1167 1577 1167
Size: 410 6054
SapI GCTCTTCn'nnn_
Cuts at: 773 2032 4487 4697 773
Size: 1259 2455 210 2540
Fragments arranged by size:
2540 2455 1259 210
SbfI CC_TGCA'GG
Cuts at: 2870 3511 2870
Size: 641 5823
ScaI AGT'ACT
Cuts at: 1237 2638 5981 1237
Size: 1401 3343 1720
Fragments arranged by size:
3343 1720 1401
SexAI A'CCwGG_T
Cuts at: 2981 2981
Size: 6464
SfcI C'TryA_G
Cuts at: 33 859 1042 1048 1144 1263 2180 2866 3507
Size: 826 183 6 96 119 917 686 641
Cuts at: 3507 4192 5185 5376 33
Size: 685 993 191 1121
Fragments arranged by size:
1121 993 917 826 686 685 641 191
183 119 96 6
SfoI GGC'GCC
Cuts at: 1896 2064 3281 3896 4144 1896
Size: 168 1217 615 248 4216
Fragments arranged by size:
4216 1217 615 248 168
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6464
SmaI CCC'GGG
Cuts at: 1435 3486 1435
Size: 2051 4413
SmlI C'TyrA_G
Cuts at: 281 345 1792 2211 3619 4534 5026 5288 281
Size: 64 1447 419 1408 915 492 262 1457
Fragments arranged by size:
1457 1447 1408 915 492 419 262 64
SpeI A'CTAG_T
Cuts at: 67 3456 67
Size: 3389 3075
SphI G_CATG'C
Cuts at: 3517 4017 3517
Size: 500 5964
StuI AGG'CCT
Cuts at: 256 256
Size: 6464
StyI C'CwwG_G
Cuts at: 107 147 327 674 792 1374 2990 3598 3952
Size: 40 180 347 118 582 1616 608 354
Cuts at: 3952 107
Size: 2619
Fragments arranged by size:
2619 1616 608 582 354 347 180 118
40
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 100 562 1978 2007 3052 4047 4180 4639 4848
Size: 462 1416 29 1045 995 133 459 209
Cuts at: 4848 100
Size: 1716
Fragments arranged by size:
1716 1416 1045 995 462 459 209 133
29
TatI w'GTAC_w
Cuts at: 125 1235 2592 2636 5979 125
Size: 1110 1357 44 3343 610
Fragments arranged by size:
3343 1357 1110 610 44
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 173 205 680 712 1187 1219 1687 1719 2816
Size: 32 475 32 475 32 468 32 1097
Cuts at: 2816 2848 5981 6013 173
Size: 32 3133 32 624
Fragments arranged by size:
3133 1097 624 475 475 468 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3352 3355 4261 3352
Size: 3 906 5555
Fragments arranged by size:
5555 906 3
XbaI T'CTAG_A
Cuts at: 3495 3495
Size: 6464
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 145 3330 3959 145
Size: 3185 629 2650
Fragments arranged by size:
3185 2650 629
XhoI C'TCGA_G
Cuts at: 345 345
Size: 6464
XmaI C'CCGG_G
Cuts at: 1433 3484 1433
Size: 2051 4413
XmnI GAAnn'nnTTC
Cuts at: 163 1666 1816 163
Size: 1503 150 4811
Fragments arranged by size:
4811 1503 150
ZraI GAC'GTC
Cuts at: 3354 3354
Size: 6464
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PspOMI PspXI PsrI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AloI AscI AvrII BfrBI BmgBI
BmtI BsiWI BsmBI BssHII DraIII FseI FspAI HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PsiI
SacII SfiI SgrAI SnaBI SrfI SspI SwaI