Restriction map for
pF1KB3457
(Circular) (Six-base) MAPSORT of: pF1KB3457.seq Check: 3287 from: 1 to: 3641
pF1KB3457 3641 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AccI GT'mk_AC
Cuts at: 679 679
Size: 3641
Acc65I G'GTAC_C
Cuts at: 657 657
Size: 3641
AclI AA'CG_TT
Cuts at: 3481 3481
Size: 3641
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 284 470 1466 1898 2251 2913 284
Size: 186 996 432 353 662 1012
Fragments arranged by size:
1012 996 662 432 353 186
AflIII A'CryG_T
Cuts at: 993 2793 3074 993
Size: 1800 281 1560
Fragments arranged by size:
1800 1560 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 563 563
Size: 3641
AlwNI CAG_nnn'CTG
Cuts at: 946 2384 946
Size: 1438 2203
ApaLI G'TGCA_C
Cuts at: 2479 2479
Size: 3641
ApoI r'AATT_y
Cuts at: 241 645 2994 3250 241
Size: 404 2349 256 632
Fragments arranged by size:
2349 632 404 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3641
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3641
AvaI C'yCGr_G
Cuts at: 212 661 212
Size: 449 3192
BamHI G'GATC_C
Cuts at: 666 666
Size: 3641
BanI G'GyrC_C
Cuts at: 657 1071 1319 1354 657
Size: 414 248 35 2944
Fragments arranged by size:
2944 414 248 35
BanII G_rGCy'C
Cuts at: 655 1685 655
Size: 1030 2611
BbeI G_GCGC'C
Cuts at: 1075 1323 1075
Size: 248 3393
BbsI GAAGACnn'nnnn_
Cuts at: 304 304
Size: 3641
BbvCI CC'TCA_GC
Cuts at: 576 576
Size: 3641
BciVI GTATCCnnnnn_n'
Cuts at: 1539 2595 3641 1539
Size: 1056 1046 1539
Fragments arranged by size:
1539 1056 1046
BclI T'GATC_A
Cuts at: 170 1161 170
Size: 991 2650
BglII A'GATC_T
Cuts at: 1156 3193 1156
Size: 2037 1604
BlpI GC'TnA_GC
Cuts at: 753 753
Size: 3641
Bme1580I G_kGCm'C
Cuts at: 1266 1359 2483 1266
Size: 93 1124 2424
Fragments arranged by size:
2424 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 853 934 1269 3413 853
Size: 81 335 2144 1081
Fragments arranged by size:
2144 1081 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2008 2008
Size: 3641
Bpu10I CC'TnA_GC
Cuts at: 576 576
Size: 3641
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 817 1696 2410 2708 817
Size: 879 714 298 1750
Fragments arranged by size:
1750 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 3345 3345
Size: 3641
BsaAI yAC'GTr
Cuts at: 994 994
Size: 3641
BsaBI GATnn'nnATC
Cuts at: 6 134 1155 3192 6
Size: 128 1021 2037 455
Fragments arranged by size:
2037 1021 455 128
BsaHI Gr'CG_yC
Cuts at: 1072 1320 2022 3518 1072
Size: 248 702 1496 1195
Fragments arranged by size:
1496 1195 702 248
BsaWI w'CCGG_w
Cuts at: 830 1351 2029 2440 2587 2861 830
Size: 521 678 411 147 274 1610
Fragments arranged by size:
1610 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 560 1066 1824 2489 2897 560
Size: 506 758 665 408 1304
Fragments arranged by size:
1304 758 665 506 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 219 219
Size: 3641
BsiEI CG_ry'CG
Cuts at: 104 1183 1229 2459 104
Size: 1079 46 1230 1286
Fragments arranged by size:
1286 1230 1079 46
BsiHKAI G_wGCw'C
Cuts at: 655 1433 1623 2483 655
Size: 778 190 860 1813
Fragments arranged by size:
1813 860 778 190
BsmI GAATG_Cn'
Cuts at: 443 443
Size: 3641
Bsp1286I G_dGCh'C
Cuts at: 655 1266 1359 1433 1623 1685 2483 655
Size: 611 93 74 190 62 798 1813
Fragments arranged by size:
1813 798 611 190 93 74 62
BspHI T'CATG_A
Cuts at: 2073 2073
Size: 3641
BspMI ACCTGCnnnn'nnnn_
Cuts at: 691 905 1207 1588 691
Size: 214 302 381 2744
Fragments arranged by size:
2744 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1933 1987 3471 3636 50
Size: 1883 54 1484 165 55
Fragments arranged by size:
1883 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1553 1553
Size: 3641
BsrFI r'CCGG_y
Cuts at: 1639 1820 1639
Size: 181 3460
BssSI C'ACGA_G
Cuts at: 1912 2620 1912
Size: 708 2933
BstAPI GCAn_nnn'nTGC
Cuts at: 1018 1018
Size: 3641
BstBI TT'CG_AA
Cuts at: 2002 2002
Size: 3641
BstYI r'GATC_y
Cuts at: 1 601 666 1133 1156 1491 1737 2043 2055
Size: 600 65 467 23 335 246 306 12
Cuts at: 2055 2141 3193 1
Size: 86 1052 449
Fragments arranged by size:
1052 600 467 449 335 306 246 86
65 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3641
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1591 1772 1935 1591
Size: 181 163 3297
Fragments arranged by size:
3297 181 163
BtsI GCAGTG_nn'
Cuts at: 884 884
Size: 3641
DraI TTT'AAA
Cuts at: 640 640
Size: 3641
DrdI GACnn_nn'nnGTC
Cuts at: 1347 2691 1347
Size: 1344 2297
EaeI y'GGCC_r
Cuts at: 200 1226 1400 1791 1818 3163 200
Size: 1026 174 391 27 1345 678
Fragments arranged by size:
1345 1026 678 391 174 27
EagI C'GGCC_G
Cuts at: 1226 1226
Size: 3641
EarI CTCTTCn'nnn_
Cuts at: 188 449 1664 1874 188
Size: 261 1215 210 1955
Fragments arranged by size:
1955 1215 261 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1849 2591 2737 3451 1849
Size: 742 146 714 2039
Fragments arranged by size:
2039 742 714 146
EcoICRI GAG'CTC
Cuts at: 653 653
Size: 3641
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 284 470 1466 1898 2251 2913 284
Size: 186 996 432 353 662 1012
Fragments arranged by size:
1012 996 662 432 353 186
EcoO109I rG'GnC_Cy
Cuts at: 780 780
Size: 3641
EcoRI G'AATT_C
Cuts at: 241 645 241
Size: 404 3237
EcoRV GAT'ATC
Cuts at: 633 633
Size: 3641
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 199 580 1660 199
Size: 381 1080 2180
Fragments arranged by size:
2180 1080 381
FspI TGC'GCA
Cuts at: 1422 1422
Size: 3641
HaeII r_GCGC'y
Cuts at: 1075 1323 2553 3324 1075
Size: 248 1230 771 1392
Fragments arranged by size:
1392 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 143 175 1483 1513 1515 1545 143
Size: 32 1308 30 2 30 2239
Fragments arranged by size:
2239 1308 32 30 30 2
HincII GTy'rAC
Cuts at: 680 680
Size: 3641
KasI G'GCGC_C
Cuts at: 1071 1319 1071
Size: 248 3393
KpnI G_GTAC'C
Cuts at: 661 661
Size: 3641
MluI A'CGCG_T
Cuts at: 3074 3074
Size: 3641
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2400 2584 2400
Size: 184 3457
MscI TGG'CCA
Cuts at: 202 1402 202
Size: 1200 2441
MslI CAynn'nnrTG
Cuts at: 1630 1757 1630
Size: 127 3514
MspA1I CmG'CkG
Cuts at: 752 1066 1426 2210 2455 752
Size: 314 360 784 245 1938
Fragments arranged by size:
1938 784 360 314 245
NaeI GCC'GGC
Cuts at: 1822 1822
Size: 3641
NarI GG'CG_CC
Cuts at: 1072 1320 1072
Size: 248 3393
NcoI C'CATG_G
Cuts at: 107 107
Size: 3641
NgoMIV G'CCGG_C
Cuts at: 1820 1820
Size: 3641
NspI r_CATG'y
Cuts at: 319 694 1194 2797 319
Size: 375 500 1603 1163
Fragments arranged by size:
1603 1163 500 375
PciI A'CATG_T
Cuts at: 2793 2793
Size: 3641
PmeI GTTT'AAAC
Cuts at: 640 640
Size: 3641
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2087 2119 2087
Size: 32 3609
PstI C_TGCA'G
Cuts at: 688 1373 688
Size: 685 2956
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3641
PvuII CAG'CTG
Cuts at: 1066 1426 1066
Size: 360 3281
RsrII CG'GwC_CG
Cuts at: 1836 1836
Size: 3641
SacI G_AGCT'C
Cuts at: 655 655
Size: 3641
SalI G'TCGA_C
Cuts at: 678 678
Size: 3641
SapI GCTCTTCn'nnn_
Cuts at: 1664 1874 1664
Size: 210 3431
SbfI CC_TGCA'GG
Cuts at: 688 688
Size: 3641
ScaI AGT'ACT
Cuts at: 3158 3158
Size: 3641
SfcI C'TryA_G
Cuts at: 33 684 1369 2337 2528 33
Size: 651 685 968 191 1146
Fragments arranged by size:
1146 968 685 651 191
SfoI GGC'GCC
Cuts at: 1073 1321 1073
Size: 248 3393
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3641
SmaI CCC'GGG
Cuts at: 663 663
Size: 3641
SmlI C'TyrA_G
Cuts at: 212 796 1711 2425 2687 212
Size: 584 915 714 262 1166
Fragments arranged by size:
1166 915 714 584 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3641
SphI G_CATG'C
Cuts at: 694 1194 694
Size: 500 3141
StyI C'CwwG_G
Cuts at: 107 438 775 1129 107
Size: 331 337 354 2619
Fragments arranged by size:
2619 354 337 331
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1224 1357 1816 2025 1224
Size: 133 459 209 2840
Fragments arranged by size:
2840 459 209 133
TatI w'GTAC_w
Cuts at: 3156 3156
Size: 3641
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3158 3190 3158
Size: 32 3609
Tth111I GACn'n_nGTC
Cuts at: 1438 1438
Size: 3641
XbaI T'CTAG_A
Cuts at: 672 672
Size: 3641
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 426 1136 426
Size: 710 2931
XhoI C'TCGA_G
Cuts at: 212 212
Size: 3641
XmaI C'CCGG_G
Cuts at: 661 661
Size: 3641
XmnI GAAnn'nnTTC
Cuts at: 245 530 245
Size: 285 3356
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlfI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BciVI BclI BglII BlpI Bme1580I BmrI
BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PmeI
PpiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AloI
ApaI AscI AvrII BaeI BcgI BfrBI BglI BmgBI
BmtI BpmI BsaXI BseRI BsiWI BsmBI BspEI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII
EcoNI FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PfoI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
ZraI