Restriction map for
pF1KB3455
(Circular) (Six-base) MAPSORT of: pF1KB3455.seq Check: 4153 from: 1 to: 3593
pF1KB3455 3593 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AccI GT'mk_AC
Cuts at: 631 631
Size: 3593
Acc65I G'GTAC_C
Cuts at: 609 609
Size: 3593
AclI AA'CG_TT
Cuts at: 3433 3433
Size: 3593
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 566 1418 1850 2597 2865 566
Size: 852 432 747 268 1294
Fragments arranged by size:
1294 852 747 432 268
AflIII A'CryG_T
Cuts at: 945 2049 3026 945
Size: 1104 977 1512
Fragments arranged by size:
1512 1104 977
AgeI A'CCGG_T
Cuts at: 1981 1981
Size: 3593
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 422 422
Size: 3593
AlwNI CAG_nnn'CTG
Cuts at: 898 2465 898
Size: 1567 2026
ApaI G_GGCC'C
Cuts at: 291 291
Size: 3593
ApaLI G'TGCA_C
Cuts at: 407 2363 407
Size: 1956 1637
ApoI r'AATT_y
Cuts at: 597 2946 3202 597
Size: 2349 256 988
Fragments arranged by size:
2349 988 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3593
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3593
AvaI C'yCGr_G
Cuts at: 284 613 284
Size: 329 3264
BamHI G'GATC_C
Cuts at: 618 618
Size: 3593
BanI G'GyrC_C
Cuts at: 609 1023 1271 1306 609
Size: 414 248 35 2896
Fragments arranged by size:
2896 414 248 35
BanII G_rGCy'C
Cuts at: 186 291 520 554 607 1637 186
Size: 105 229 34 53 1030 2142
Fragments arranged by size:
2142 1030 229 105 53 34
BbeI G_GCGC'C
Cuts at: 1027 1275 1027
Size: 248 3345
BciVI GTATCCnnnnn_n'
Cuts at: 1491 2252 3593 1491
Size: 761 1341 1491
Fragments arranged by size:
1491 1341 761
BclI T'GATC_A
Cuts at: 1113 1113
Size: 3593
BglII A'GATC_T
Cuts at: 1108 3145 1108
Size: 2037 1556
BlpI GC'TnA_GC
Cuts at: 705 705
Size: 3593
Bme1580I G_kGCm'C
Cuts at: 291 411 435 1218 1311 2367 291
Size: 120 24 783 93 1056 1517
Fragments arranged by size:
1517 1056 783 120 93 24
BmrI ACTGGGnnnn_n'
Cuts at: 805 886 1221 3365 805
Size: 81 335 2144 1033
Fragments arranged by size:
2144 1033 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1960 1960
Size: 3593
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 412 433 510 412
Size: 21 77 3495
Fragments arranged by size:
3495 77 21
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 355 769 1648 2140 2438 355
Size: 414 879 492 298 1510
Fragments arranged by size:
1510 879 492 414 298
BsaI GGTCTCn'nnnn_
Cuts at: 3297 3297
Size: 3593
BsaAI yAC'GTr
Cuts at: 946 946
Size: 3593
BsaBI GATnn'nnATC
Cuts at: 6 1107 3144 6
Size: 1101 2037 455
Fragments arranged by size:
2037 1101 455
BsaHI Gr'CG_yC
Cuts at: 1024 1272 1974 3470 1024
Size: 248 702 1496 1147
Fragments arranged by size:
1496 1147 702 248
BsaWI w'CCGG_w
Cuts at: 572 782 1303 1981 2255 2402 2813 572
Size: 210 521 678 274 147 411 1352
Fragments arranged by size:
1352 678 521 411 274 210 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 439 469 439
Size: 30 3563
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 447 447
Size: 3593
BseYI C'CCAG_C
Cuts at: 1018 1776 2353 2849 1018
Size: 758 577 496 1762
Fragments arranged by size:
1762 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1135 1181 2389 104
Size: 1031 46 1208 1308
Fragments arranged by size:
1308 1208 1031 46
BsiHKAI G_wGCw'C
Cuts at: 411 607 1385 1575 2367 411
Size: 196 778 190 792 1637
Fragments arranged by size:
1637 792 778 196 190
BsmI GAATG_Cn'
Cuts at: 387 387
Size: 3593
Bsp1286I G_dGCh'C
Cuts at: 186 291 411 435 520 554 607 1218 1311
Size: 105 120 24 85 34 53 611 93
Cuts at: 1311 1385 1575 1637 2367 186
Size: 74 190 62 730 1412
Fragments arranged by size:
1412 730 611 190 120 105 93 85
74 62 53 34 24
BspEI T'CCGG_A
Cuts at: 572 2813 572
Size: 2241 1352
BspHI T'CATG_A
Cuts at: 2769 2769
Size: 3593
BspMI ACCTGCnnnn'nnnn_
Cuts at: 643 857 1159 1540 643
Size: 214 302 381 2696
Fragments arranged by size:
2696 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1885 1939 3423 3588 50
Size: 1835 54 1484 165 55
Fragments arranged by size:
1835 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1505 1505
Size: 3593
BsrFI r'CCGG_y
Cuts at: 1591 1772 1981 1591
Size: 181 209 3203
Fragments arranged by size:
3203 209 181
BssSI C'ACGA_G
Cuts at: 1864 2222 1864
Size: 358 3235
BstAPI GCAn_nnn'nTGC
Cuts at: 970 970
Size: 3593
BstBI TT'CG_AA
Cuts at: 1954 1954
Size: 3593
BstYI r'GATC_y
Cuts at: 1 618 1085 1108 1443 1689 2701 2787 2799
Size: 617 467 23 335 246 1012 86 12
Cuts at: 2799 3145 1
Size: 346 449
Fragments arranged by size:
1012 617 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1543 1724 1887 1543
Size: 181 163 3249
Fragments arranged by size:
3249 181 163
BtsI GCAGTG_nn'
Cuts at: 836 836
Size: 3593
DraI TTT'AAA
Cuts at: 592 592
Size: 3593
DrdI GACnn_nn'nnGTC
Cuts at: 1299 2157 1299
Size: 858 2735
EaeI y'GGCC_r
Cuts at: 1178 1352 1743 1770 3115 1178
Size: 174 391 27 1345 1656
Fragments arranged by size:
1656 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1178 1178
Size: 3593
EarI CTCTTCn'nnn_
Cuts at: 140 392 542 1616 1826 140
Size: 252 150 1074 210 1907
Fragments arranged by size:
1907 1074 252 210 150
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1801 2111 2257 3403 1801
Size: 310 146 1146 1991
Fragments arranged by size:
1991 1146 310 146
EcoICRI GAG'CTC
Cuts at: 605 605
Size: 3593
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 412 433 510 566 1418 1850 2597 2865 412
Size: 21 77 56 852 432 747 268 1140
Fragments arranged by size:
1140 852 747 432 268 77 56 21
EcoO109I rG'GnC_Cy
Cuts at: 732 732
Size: 3593
EcoRI G'AATT_C
Cuts at: 597 597
Size: 3593
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1612 1612
Size: 3593
FspI TGC'GCA
Cuts at: 1374 1374
Size: 3593
HaeII r_GCGC'y
Cuts at: 1027 1275 2297 3276 1027
Size: 248 1022 979 1344
Fragments arranged by size:
1344 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 265 297 502 514 534 546 1435 1465 1467
Size: 32 205 12 20 12 889 30 2
Cuts at: 1467 1497 265
Size: 30 2361
Fragments arranged by size:
2361 889 205 32 30 30 20 12
12 2
HincII GTy'rAC
Cuts at: 632 632
Size: 3593
KasI G'GCGC_C
Cuts at: 1023 1271 1023
Size: 248 3345
KpnI G_GTAC'C
Cuts at: 613 613
Size: 3593
MluI A'CGCG_T
Cuts at: 3026 3026
Size: 3593
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2264 2448 2264
Size: 184 3409
MscI TGG'CCA
Cuts at: 1354 1354
Size: 3593
MslI CAynn'nnrTG
Cuts at: 130 191 1582 1709 130
Size: 61 1391 127 2014
Fragments arranged by size:
2014 1391 127 61
MspA1I CmG'CkG
Cuts at: 704 1018 1378 2391 2636 704
Size: 314 360 1013 245 1661
Fragments arranged by size:
1661 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1774 1774
Size: 3593
NarI GG'CG_CC
Cuts at: 1024 1272 1024
Size: 248 3345
NgoMIV G'CCGG_C
Cuts at: 1772 1772
Size: 3593
NspI r_CATG'y
Cuts at: 646 1146 2053 646
Size: 500 907 2186
Fragments arranged by size:
2186 907 500
PciI A'CATG_T
Cuts at: 2049 2049
Size: 3593
PmeI GTTT'AAAC
Cuts at: 592 592
Size: 3593
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2732 2764 2732
Size: 32 3561
PspOMI G'GGCC_C
Cuts at: 287 287
Size: 3593
PstI C_TGCA'G
Cuts at: 443 640 1325 443
Size: 197 685 2711
Fragments arranged by size:
2711 685 197
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3593
PvuII CAG'CTG
Cuts at: 1018 1378 1018
Size: 360 3233
RsrII CG'GwC_CG
Cuts at: 1788 1788
Size: 3593
SacI G_AGCT'C
Cuts at: 607 607
Size: 3593
SalI G'TCGA_C
Cuts at: 630 630
Size: 3593
SapI GCTCTTCn'nnn_
Cuts at: 140 392 542 1616 1826 140
Size: 252 150 1074 210 1907
Fragments arranged by size:
1907 1074 252 210 150
SbfI CC_TGCA'GG
Cuts at: 640 640
Size: 3593
ScaI AGT'ACT
Cuts at: 3110 3110
Size: 3593
SfcI C'TryA_G
Cuts at: 33 439 636 1321 2314 2505 33
Size: 406 197 685 993 191 1121
Fragments arranged by size:
1121 993 685 406 197 191
SfoI GGC'GCC
Cuts at: 1025 1273 1025
Size: 248 3345
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3593
SmaI CCC'GGG
Cuts at: 286 615 286
Size: 329 3264
SmlI C'TyrA_G
Cuts at: 370 748 1663 2155 2417 370
Size: 378 915 492 262 1546
Fragments arranged by size:
1546 915 492 378 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3593
SphI G_CATG'C
Cuts at: 646 1146 646
Size: 500 3093
StyI C'CwwG_G
Cuts at: 267 485 727 1081 267
Size: 218 242 354 2779
Fragments arranged by size:
2779 354 242 218
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1176 1309 1768 1977 1176
Size: 133 459 209 2792
Fragments arranged by size:
2792 459 209 133
TatI w'GTAC_w
Cuts at: 3108 3108
Size: 3593
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3110 3142 3110
Size: 32 3561
Tth111I GACn'n_nGTC
Cuts at: 253 1390 253
Size: 1137 2456
XbaI T'CTAG_A
Cuts at: 624 624
Size: 3593
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1088 1088
Size: 3593
XmaI C'CCGG_G
Cuts at: 284 613 284
Size: 329 3264
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NspI PciI PmeI PpiI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BglI BmgBI
BmtI Bpu10I BsgI BsiWI BsmBI BsrGI BssHII BstEII
BstXI BstZ17I Bsu36I BtgI ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NcoI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PfoI
PmlI PpuMI PshAI PsiI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI