Restriction map for
pF1KB3451
(Circular) (Six-base) MAPSORT of: pF1KB3451.seq Check: 4457 from: 1 to: 3905
pF1KB3451 3905 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 222 222
Size: 3905
AccI GT'mk_AC
Cuts at: 943 943
Size: 3905
Acc65I G'GTAC_C
Cuts at: 921 921
Size: 3905
AclI AA'CG_TT
Cuts at: 3745 3745
Size: 3905
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 392 781 1730 2162 2909 3177 392
Size: 389 949 432 747 268 1120
Fragments arranged by size:
1120 949 747 432 389 268
AflIII A'CryG_T
Cuts at: 1257 2361 3338 1257
Size: 1104 977 1824
Fragments arranged by size:
1824 1104 977
AgeI A'CCGG_T
Cuts at: 2293 2293
Size: 3905
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 869 901 869
Size: 32 3873
AlwNI CAG_nnn'CTG
Cuts at: 1210 2777 1210
Size: 1567 2338
ApaLI G'TGCA_C
Cuts at: 2675 2675
Size: 3905
ApoI r'AATT_y
Cuts at: 355 909 3258 3514 355
Size: 554 2349 256 746
Fragments arranged by size:
2349 746 554 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3905
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3905
AvaI C'yCGr_G
Cuts at: 626 925 626
Size: 299 3606
BamHI G'GATC_C
Cuts at: 930 930
Size: 3905
BanI G'GyrC_C
Cuts at: 921 1335 1583 1618 921
Size: 414 248 35 3208
Fragments arranged by size:
3208 414 248 35
BanII G_rGCy'C
Cuts at: 919 1949 919
Size: 1030 2875
BbeI G_GCGC'C
Cuts at: 1339 1587 1339
Size: 248 3657
BbsI GAAGACnn'nnnn_
Cuts at: 568 729 568
Size: 161 3744
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 829 863 829
Size: 34 3871
BciVI GTATCCnnnnn_n'
Cuts at: 1803 2564 3905 1803
Size: 761 1341 1803
Fragments arranged by size:
1803 1341 761
BclI T'GATC_A
Cuts at: 1425 1425
Size: 3905
BglII A'GATC_T
Cuts at: 1420 3457 1420
Size: 2037 1868
BlpI GC'TnA_GC
Cuts at: 575 1017 575
Size: 442 3463
Bme1580I G_kGCm'C
Cuts at: 1530 1623 2679 1530
Size: 93 1056 2756
Fragments arranged by size:
2756 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 842 1117 1198 1533 3677 842
Size: 275 81 335 2144 1070
Fragments arranged by size:
2144 1070 335 275 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2272 2272
Size: 3905
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 277 1081 1960 2452 2750 277
Size: 804 879 492 298 1432
Fragments arranged by size:
1432 879 804 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 295 3609 295
Size: 3314 591
BsaAI yAC'GTr
Cuts at: 1258 1258
Size: 3905
BsaBI GATnn'nnATC
Cuts at: 6 616 1419 3456 6
Size: 610 803 2037 455
Fragments arranged by size:
2037 803 610 455
BsaHI Gr'CG_yC
Cuts at: 1336 1584 2286 3782 1336
Size: 248 702 1496 1459
Fragments arranged by size:
1496 1459 702 248
BsaWI w'CCGG_w
Cuts at: 599 1094 1615 2293 2567 2714 3125 599
Size: 495 521 678 274 147 411 1379
Fragments arranged by size:
1379 678 521 495 411 274 147
BseYI C'CCAG_C
Cuts at: 1330 2088 2665 3161 1330
Size: 758 577 496 2074
Fragments arranged by size:
2074 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 655 655
Size: 3905
BsiEI CG_ry'CG
Cuts at: 104 1447 1493 2701 104
Size: 1343 46 1208 1308
Fragments arranged by size:
1343 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 919 1697 1887 2679 919
Size: 778 190 792 2145
Fragments arranged by size:
2145 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 919 1530 1623 1697 1887 1949 2679 919
Size: 611 93 74 190 62 730 2145
Fragments arranged by size:
2145 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 599 3125 599
Size: 2526 1379
BspHI T'CATG_A
Cuts at: 620 3081 620
Size: 2461 1444
BspMI ACCTGCnnnn'nnnn_
Cuts at: 222 955 1169 1471 1852 222
Size: 733 214 302 381 2275
Fragments arranged by size:
2275 733 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2197 2251 3735 3900 50
Size: 2147 54 1484 165 55
Fragments arranged by size:
2147 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1817 1817
Size: 3905
BsrFI r'CCGG_y
Cuts at: 1903 2084 2293 1903
Size: 181 209 3515
Fragments arranged by size:
3515 209 181
BssSI C'ACGA_G
Cuts at: 2176 2534 2176
Size: 358 3547
BstAPI GCAn_nnn'nTGC
Cuts at: 1282 1282
Size: 3905
BstBI TT'CG_AA
Cuts at: 2266 2266
Size: 3905
BstYI r'GATC_y
Cuts at: 1 417 930 1397 1420 1755 2001 3013 3099
Size: 416 513 467 23 335 246 1012 86
Cuts at: 3099 3111 3457 1
Size: 12 346 449
Fragments arranged by size:
1012 513 467 449 416 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 408 443 107
Size: 301 35 3569
Fragments arranged by size:
3569 301 35
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1855 2036 2199 1855
Size: 181 163 3561
Fragments arranged by size:
3561 181 163
BtsI GCAGTG_nn'
Cuts at: 1148 1148
Size: 3905
DraI TTT'AAA
Cuts at: 904 904
Size: 3905
DrdI GACnn_nn'nnGTC
Cuts at: 1611 2469 1611
Size: 858 3047
EaeI y'GGCC_r
Cuts at: 1490 1664 2055 2082 3427 1490
Size: 174 391 27 1345 1968
Fragments arranged by size:
1968 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1490 1490
Size: 3905
EarI CTCTTCn'nnn_
Cuts at: 1928 2138 1928
Size: 210 3695
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 580 2113 2423 2569 3715 580
Size: 1533 310 146 1146 770
Fragments arranged by size:
1533 1146 770 310 146
EcoICRI GAG'CTC
Cuts at: 917 917
Size: 3905
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 392 781 1730 2162 2909 3177 392
Size: 389 949 432 747 268 1120
Fragments arranged by size:
1120 949 747 432 389 268
EcoO109I rG'GnC_Cy
Cuts at: 260 1044 260
Size: 784 3121
EcoRI G'AATT_C
Cuts at: 909 909
Size: 3905
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 334 1924 334
Size: 1590 2315
FspI TGC'GCA
Cuts at: 1686 1686
Size: 3905
HaeII r_GCGC'y
Cuts at: 132 1339 1587 2609 3588 132
Size: 1207 248 1022 979 449
Fragments arranged by size:
1207 1022 979 449 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 459 491 1747 1777 1779 1809 459
Size: 32 1256 30 2 30 2555
Fragments arranged by size:
2555 1256 32 30 30 2
HincII GTy'rAC
Cuts at: 944 944
Size: 3905
KasI G'GCGC_C
Cuts at: 1335 1583 1335
Size: 248 3657
KpnI G_GTAC'C
Cuts at: 925 925
Size: 3905
MluI A'CGCG_T
Cuts at: 3338 3338
Size: 3905
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2576 2760 2576
Size: 184 3721
MscI TGG'CCA
Cuts at: 1666 1666
Size: 3905
MslI CAynn'nnrTG
Cuts at: 482 1894 2021 482
Size: 1412 127 2366
Fragments arranged by size:
2366 1412 127
MspA1I CmG'CkG
Cuts at: 1016 1330 1690 2703 2948 1016
Size: 314 360 1013 245 1973
Fragments arranged by size:
1973 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2086 2086
Size: 3905
NarI GG'CG_CC
Cuts at: 1336 1584 1336
Size: 248 3657
NcoI C'CATG_G
Cuts at: 107 408 443 107
Size: 301 35 3569
Fragments arranged by size:
3569 301 35
NgoMIV G'CCGG_C
Cuts at: 2084 2084
Size: 3905
NspI r_CATG'y
Cuts at: 958 1458 2365 958
Size: 500 907 2498
Fragments arranged by size:
2498 907 500
PciI A'CATG_T
Cuts at: 2361 2361
Size: 3905
PflMI CCAn_nnn'nTGG
Cuts at: 414 414
Size: 3905
PmeI GTTT'AAAC
Cuts at: 904 904
Size: 3905
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3044 3076 3044
Size: 32 3873
PpuMI rG'GwC_Cy
Cuts at: 260 260
Size: 3905
PshAI GACnn'nnGTC
Cuts at: 250 250
Size: 3905
PstI C_TGCA'G
Cuts at: 952 1637 952
Size: 685 3220
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3905
PvuII CAG'CTG
Cuts at: 1330 1690 1330
Size: 360 3545
RsrII CG'GwC_CG
Cuts at: 2100 2100
Size: 3905
SacI G_AGCT'C
Cuts at: 919 919
Size: 3905
SalI G'TCGA_C
Cuts at: 942 942
Size: 3905
SapI GCTCTTCn'nnn_
Cuts at: 1928 2138 1928
Size: 210 3695
SbfI CC_TGCA'GG
Cuts at: 952 952
Size: 3905
ScaI AGT'ACT
Cuts at: 3422 3422
Size: 3905
SfcI C'TryA_G
Cuts at: 33 948 1633 2626 2817 33
Size: 915 685 993 191 1121
Fragments arranged by size:
1121 993 915 685 191
SfoI GGC'GCC
Cuts at: 1337 1585 1337
Size: 248 3657
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3905
SmaI CCC'GGG
Cuts at: 927 927
Size: 3905
SmlI C'TyrA_G
Cuts at: 292 1060 1975 2467 2729 292
Size: 768 915 492 262 1468
Fragments arranged by size:
1468 915 768 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3905
SphI G_CATG'C
Cuts at: 958 1458 958
Size: 500 3405
SspI AAT'ATT
Cuts at: 237 237
Size: 3905
StyI C'CwwG_G
Cuts at: 107 264 408 443 1039 1393 107
Size: 157 144 35 596 354 2619
Fragments arranged by size:
2619 596 354 157 144 35
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1488 1621 2080 2289 1488
Size: 133 459 209 3104
Fragments arranged by size:
3104 459 209 133
TatI w'GTAC_w
Cuts at: 3420 3420
Size: 3905
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3422 3454 3422
Size: 32 3873
Tth111I GACn'n_nGTC
Cuts at: 1702 1702
Size: 3905
XbaI T'CTAG_A
Cuts at: 936 936
Size: 3905
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 165 777 1400 165
Size: 612 623 2670
Fragments arranged by size:
2670 623 612
XmaI C'CCGG_G
Cuts at: 925 925
Size: 3905
XmnI GAAnn'nnTTC
Cuts at: 377 377
Size: 3905
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI
PmeI PpiI PpuMI PshAI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI ApaI AscI
AvrII BaeI BbvCI BfrBI BglI BmgBI BmtI BpmI
Bpu10I BsaXI BseRI BsiWI BsmI BsmBI BsrGI BssHII
BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PfoI PmlI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI StuI SwaI XhoI ZraI