Restriction map for
pF1KB3450
(Circular) (Six-base) MAPSORT of: pF1KB3450.seq Check: 4486 from: 1 to: 3992
pF1KB3450 3992 bp
With 182 enzymes: SgfI *
April 24, 2009 13:01 ..
AccI GT'mk_AC
Cuts at: 1030 1030
Size: 3992
Acc65I G'GTAC_C
Cuts at: 1008 1008
Size: 3992
AclI AA'CG_TT
Cuts at: 3832 3832
Size: 3992
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1817 2249 2996 3264 1817
Size: 432 747 268 2545
Fragments arranged by size:
2545 747 432 268
AflIII A'CryG_T
Cuts at: 1344 2448 3425 1344
Size: 1104 977 1911
Fragments arranged by size:
1911 1104 977
AgeI A'CCGG_T
Cuts at: 2380 2380
Size: 3992
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 473 473
Size: 3992
AlwNI CAG_nnn'CTG
Cuts at: 1297 2864 1297
Size: 1567 2425
ApaI G_GGCC'C
Cuts at: 140 148 140
Size: 8 3984
ApaLI G'TGCA_C
Cuts at: 2762 2762
Size: 3992
ApoI r'AATT_y
Cuts at: 996 3345 3601 996
Size: 2349 256 1387
Fragments arranged by size:
2349 1387 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3992
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3992
AvaI C'yCGr_G
Cuts at: 139 785 1012 139
Size: 646 227 3119
Fragments arranged by size:
3119 646 227
BamHI G'GATC_C
Cuts at: 1017 1017
Size: 3992
BanI G'GyrC_C
Cuts at: 1008 1422 1670 1705 1008
Size: 414 248 35 3295
Fragments arranged by size:
3295 414 248 35
BanII G_rGCy'C
Cuts at: 140 148 258 1006 2036 140
Size: 8 110 748 1030 2096
Fragments arranged by size:
2096 1030 748 110 8
BbeI G_GCGC'C
Cuts at: 1426 1674 1426
Size: 248 3744
BbvCI CC'TCA_GC
Cuts at: 780 780
Size: 3992
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 699 733 699
Size: 34 3958
BciVI GTATCCnnnnn_n'
Cuts at: 1890 2651 3992 1890
Size: 761 1341 1890
Fragments arranged by size:
1890 1341 761
BclI T'GATC_A
Cuts at: 1512 1512
Size: 3992
BglI GCCn_nnn'nGGC
Cuts at: 152 671 152
Size: 519 3473
BglII A'GATC_T
Cuts at: 347 1507 3544 347
Size: 1160 2037 795
Fragments arranged by size:
2037 1160 795
BlpI GC'TnA_GC
Cuts at: 1104 1104
Size: 3992
Bme1580I G_kGCm'C
Cuts at: 140 148 712 1617 1710 2766 140
Size: 8 564 905 93 1056 1366
Fragments arranged by size:
1366 1056 905 564 93 8
BmgBI CAC'GTC
Cuts at: 516 516
Size: 3992
BmrI ACTGGGnnnn_n'
Cuts at: 624 1204 1285 1620 3764 624
Size: 580 81 335 2144 852
Fragments arranged by size:
2144 852 580 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2359 2359
Size: 3992
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 361 953 361
Size: 592 3400
Bpu10I CC'TnA_GC
Cuts at: 780 780
Size: 3992
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 397 1168 2047 2539 2837 397
Size: 771 879 492 298 1552
Fragments arranged by size:
1552 879 771 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3696 3696
Size: 3992
BsaAI yAC'GTr
Cuts at: 1345 1345
Size: 3992
BsaBI GATnn'nnATC
Cuts at: 6 1506 3543 6
Size: 1500 2037 455
Fragments arranged by size:
2037 1500 455
BsaHI Gr'CG_yC
Cuts at: 119 1423 1671 2373 3869 119
Size: 1304 248 702 1496 242
Fragments arranged by size:
1496 1304 702 248 242
BsaWI w'CCGG_w
Cuts at: 1181 1702 2380 2654 2801 3212 1181
Size: 521 678 274 147 411 1961
Fragments arranged by size:
1961 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 882 912 951 981 882
Size: 30 39 30 3893
Fragments arranged by size:
3893 39 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 358 358
Size: 3992
BseYI C'CCAG_C
Cuts at: 1417 2175 2752 3248 1417
Size: 758 577 496 2161
Fragments arranged by size:
2161 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 146 463 683 777 973 146
Size: 317 220 94 196 3165
Fragments arranged by size:
3165 317 220 196 94
BsiEI CG_ry'CG
Cuts at: 104 225 1534 1580 2788 104
Size: 121 1309 46 1208 1308
Fragments arranged by size:
1309 1308 1208 121 46
BsiHKAI G_wGCw'C
Cuts at: 258 538 1006 1784 1974 2766 258
Size: 280 468 778 190 792 1484
Fragments arranged by size:
1484 792 778 468 280 190
BsmBI CGTCTCn'nnnn_
Cuts at: 726 726
Size: 3992
Bsp1286I G_dGCh'C
Cuts at: 140 148 258 538 712 1006 1617 1710 1784
Size: 8 110 280 174 294 611 93 74
Cuts at: 1784 1974 2036 2766 140
Size: 190 62 730 1366
Fragments arranged by size:
1366 730 611 294 280 190 174 110
93 74 62 8
BspEI T'CCGG_A
Cuts at: 3212 3212
Size: 3992
BspHI T'CATG_A
Cuts at: 749 3168 749
Size: 2419 1573
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1042 1256 1558 1939 1042
Size: 214 302 381 3095
Fragments arranged by size:
3095 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2284 2338 3822 3987 50
Size: 2234 54 1484 165 55
Fragments arranged by size:
2234 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1904 1904
Size: 3992
BsrFI r'CCGG_y
Cuts at: 121 460 1990 2171 2380 121
Size: 339 1530 181 209 1733
Fragments arranged by size:
1733 1530 339 209 181
BssSI C'ACGA_G
Cuts at: 2263 2621 2263
Size: 358 3634
BstAPI GCAn_nnn'nTGC
Cuts at: 490 1369 490
Size: 879 3113
BstBI TT'CG_AA
Cuts at: 2353 2353
Size: 3992
BstXI CCAn_nnnn'nTGG
Cuts at: 546 576 546
Size: 30 3962
BstYI r'GATC_y
Cuts at: 1 347 1017 1484 1507 1842 2088 3100 3186
Size: 346 670 467 23 335 246 1012 86
Cuts at: 3186 3198 3544 1
Size: 12 346 449
Fragments arranged by size:
1012 670 467 449 346 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 171 183 225 249 561 107
Size: 64 12 42 24 312 3538
Fragments arranged by size:
3538 312 64 42 24 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1942 2123 2286 1942
Size: 181 163 3648
Fragments arranged by size:
3648 181 163
BtsI GCAGTG_nn'
Cuts at: 204 794 1235 204
Size: 590 441 2961
Fragments arranged by size:
2961 590 441
ClaI AT'CG_AT
Cuts at: 764 764
Size: 3992
DraI TTT'AAA
Cuts at: 991 991
Size: 3992
DrdI GACnn_nn'nnGTC
Cuts at: 925 1698 2556 925
Size: 773 858 2361
Fragments arranged by size:
2361 858 773
EaeI y'GGCC_r
Cuts at: 222 462 510 548 578 1577 1751 2142 2169
Size: 240 48 38 30 999 174 391 27
Cuts at: 2169 3514 222
Size: 1345 700
Fragments arranged by size:
1345 999 700 391 240 174 48 38
30 27
EagI C'GGCC_G
Cuts at: 222 1577 222
Size: 1355 2637
EarI CTCTTCn'nnn_
Cuts at: 361 2015 2225 361
Size: 1654 210 2128
Fragments arranged by size:
2128 1654 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 127 2200 2510 2656 3802 127
Size: 2073 310 146 1146 317
Fragments arranged by size:
2073 1146 317 310 146
EcoICRI GAG'CTC
Cuts at: 256 1004 256
Size: 748 3244
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 361 953 1817 2249 2996 3264 361
Size: 592 864 432 747 268 1089
Fragments arranged by size:
1089 864 747 592 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 874 874
Size: 3992
EcoO109I rG'GnC_Cy
Cuts at: 136 144 145 404 1131 136
Size: 8 1 259 727 2997
Fragments arranged by size:
2997 727 259 8 1
EcoRI G'AATT_C
Cuts at: 996 996
Size: 3992
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 843 2011 843
Size: 1168 2824
FspI TGC'GCA
Cuts at: 604 1773 604
Size: 1169 2823
HaeII r_GCGC'y
Cuts at: 1426 1674 2696 3675 1426
Size: 248 1022 979 1743
Fragments arranged by size:
1743 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 951 983 1834 1864 1866 1896 951
Size: 32 851 30 2 30 3047
Fragments arranged by size:
3047 851 32 30 30 2
HincII GTy'rAC
Cuts at: 1031 1031
Size: 3992
KasI G'GCGC_C
Cuts at: 1422 1670 1422
Size: 248 3744
KpnI G_GTAC'C
Cuts at: 1012 1012
Size: 3992
MluI A'CGCG_T
Cuts at: 3425 3425
Size: 3992
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2663 2847 2663
Size: 184 3808
MscI TGG'CCA
Cuts at: 512 550 580 1753 512
Size: 38 30 1173 2751
Fragments arranged by size:
2751 1173 38 30
MslI CAynn'nnrTG
Cuts at: 1981 2108 1981
Size: 127 3865
MspA1I CmG'CkG
Cuts at: 130 173 185 251 668 957 973 1103 1417
Size: 43 12 66 417 289 16 130 314
Cuts at: 1417 1777 2790 3035 130
Size: 360 1013 245 1087
Fragments arranged by size:
1087 1013 417 360 314 289 245 130
66 43 16 12
NaeI GCC'GGC
Cuts at: 462 2173 462
Size: 1711 2281
NarI GG'CG_CC
Cuts at: 1423 1671 1423
Size: 248 3744
NcoI C'CATG_G
Cuts at: 107 107
Size: 3992
NgoMIV G'CCGG_C
Cuts at: 460 2171 460
Size: 1711 2281
NspI r_CATG'y
Cuts at: 1045 1545 2452 1045
Size: 500 907 2585
Fragments arranged by size:
2585 907 500
PasI CC'CwG_GG
Cuts at: 157 704 804 157
Size: 547 100 3345
Fragments arranged by size:
3345 547 100
PciI A'CATG_T
Cuts at: 2448 2448
Size: 3992
PmeI GTTT'AAAC
Cuts at: 991 991
Size: 3992
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3131 3163 3131
Size: 32 3960
PspOMI G'GGCC_C
Cuts at: 136 144 136
Size: 8 3984
PstI C_TGCA'G
Cuts at: 1039 1724 1039
Size: 685 3307
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3992
PvuII CAG'CTG
Cuts at: 973 1417 1777 973
Size: 444 360 3188
Fragments arranged by size:
3188 444 360
RsrII CG'GwC_CG
Cuts at: 2187 2187
Size: 3992
SacI G_AGCT'C
Cuts at: 258 1006 258
Size: 748 3244
SacII CC_GC'GG
Cuts at: 174 186 252 174
Size: 12 66 3914
Fragments arranged by size:
3914 66 12
SalI G'TCGA_C
Cuts at: 1029 1029
Size: 3992
SapI GCTCTTCn'nnn_
Cuts at: 2015 2225 2015
Size: 210 3782
SbfI CC_TGCA'GG
Cuts at: 1039 1039
Size: 3992
ScaI AGT'ACT
Cuts at: 3509 3509
Size: 3992
SexAI A'CCwGG_T
Cuts at: 472 472
Size: 3992
SfcI C'TryA_G
Cuts at: 33 849 1035 1720 2713 2904 33
Size: 816 186 685 993 191 1121
Fragments arranged by size:
1121 993 816 685 191 186
SfiI GGCCn_nnn'nGGCC
Cuts at: 152 152
Size: 3992
SfoI GGC'GCC
Cuts at: 1424 1672 1424
Size: 248 3744
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3992
SgrAI Cr'CCGG_yG
Cuts at: 121 121
Size: 3992
SmaI CCC'GGG
Cuts at: 141 787 1014 141
Size: 646 227 3119
Fragments arranged by size:
3119 646 227
SmlI C'TyrA_G
Cuts at: 412 1147 2062 2554 2816 412
Size: 735 915 492 262 1588
Fragments arranged by size:
1588 915 735 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3992
SphI G_CATG'C
Cuts at: 1045 1545 1045
Size: 500 3492
StyI C'CwwG_G
Cuts at: 107 539 818 1126 1480 107
Size: 432 279 308 354 2619
Fragments arranged by size:
2619 432 354 308 279
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 508 514 717 1575 1708 2167 2376 508
Size: 6 203 858 133 459 209 2124
Fragments arranged by size:
2124 858 459 209 203 133 6
TatI w'GTAC_w
Cuts at: 3507 3507
Size: 3992
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 159 191 268 300 882 914 3509 3541 159
Size: 32 77 32 582 32 2595 32 610
Fragments arranged by size:
2595 610 582 77 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1789 1789
Size: 3992
XbaI T'CTAG_A
Cuts at: 1023 1023
Size: 3992
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1487 1487
Size: 3992
XmaI C'CCGG_G
Cuts at: 139 785 1012 139
Size: 646 227 3119
Fragments arranged by size:
3119 646 227
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI ClaI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PmeI PpiI PspOMI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI AscI
AvrII BaeI BbsI BfrBI BmtI BsiWI BsmI BsrGI
BssHII BstEII BstZ17I Bsu36I CspCI DraIII EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PflMI PfoI PmlI PpuMI PshAI PsiI
PspXI PsrI SanDI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI