Restriction map for
pF1KB3448
(Circular) (Six-base) MAPSORT of: pF1KB3448.seq Check: 3679 from: 1 to: 4001
pF1KB3448 4001 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 544 544
Size: 4001
AccI GT'mk_AC
Cuts at: 1039 1039
Size: 4001
Acc65I G'GTAC_C
Cuts at: 1017 1017
Size: 4001
AclI AA'CG_TT
Cuts at: 3841 3841
Size: 4001
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 616 1826 2258 3005 3273 616
Size: 1210 432 747 268 1344
Fragments arranged by size:
1344 1210 747 432 268
AflIII A'CryG_T
Cuts at: 1353 2457 3434 1353
Size: 1104 977 1920
Fragments arranged by size:
1920 1104 977
AgeI A'CCGG_T
Cuts at: 2389 2389
Size: 4001
AlwNI CAG_nnn'CTG
Cuts at: 1306 2873 1306
Size: 1567 2434
ApaLI G'TGCA_C
Cuts at: 2771 2771
Size: 4001
ApoI r'AATT_y
Cuts at: 163 673 1005 3354 3610 163
Size: 510 332 2349 256 554
Fragments arranged by size:
2349 554 510 332 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4001
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4001
AvaI C'yCGr_G
Cuts at: 1021 1021
Size: 4001
BamHI G'GATC_C
Cuts at: 1026 1026
Size: 4001
BanI G'GyrC_C
Cuts at: 526 589 1017 1431 1679 1714 526
Size: 63 428 414 248 35 2813
Fragments arranged by size:
2813 428 414 248 63 35
BanII G_rGCy'C
Cuts at: 975 1015 2045 975
Size: 40 1030 2931
Fragments arranged by size:
2931 1030 40
BbeI G_GCGC'C
Cuts at: 1435 1683 1435
Size: 248 3753
BbsI GAAGACnn'nnnn_
Cuts at: 177 177
Size: 4001
BciVI GTATCCnnnnn_n'
Cuts at: 1899 2660 4001 1899
Size: 761 1341 1899
Fragments arranged by size:
1899 1341 761
BclI T'GATC_A
Cuts at: 1521 1521
Size: 4001
BglII A'GATC_T
Cuts at: 458 1516 3553 458
Size: 1058 2037 906
Fragments arranged by size:
2037 1058 906
BlpI GC'TnA_GC
Cuts at: 1113 1113
Size: 4001
Bme1580I G_kGCm'C
Cuts at: 594 1626 1719 2775 594
Size: 1032 93 1056 1820
Fragments arranged by size:
1820 1056 1032 93
BmrI ACTGGGnnnn_n'
Cuts at: 1213 1294 1629 3773 1213
Size: 81 335 2144 1441
Fragments arranged by size:
2144 1441 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2368 2368
Size: 4001
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 788 788
Size: 4001
Bpu10I CC'TnA_GC
Cuts at: 974 974
Size: 4001
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1177 2056 2548 2846 1177
Size: 879 492 298 2332
Fragments arranged by size:
2332 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3705 3705
Size: 4001
BsaAI yAC'GTr
Cuts at: 1354 1354
Size: 4001
BsaBI GATnn'nnATC
Cuts at: 6 1515 3552 6
Size: 1509 2037 455
Fragments arranged by size:
2037 1509 455
BsaHI Gr'CG_yC
Cuts at: 1432 1680 2382 3878 1432
Size: 248 702 1496 1555
Fragments arranged by size:
1555 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1190 1711 2389 2663 2810 3221 1190
Size: 521 678 274 147 411 1970
Fragments arranged by size:
1970 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 805 835 805
Size: 30 3971
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 829 829
Size: 4001
BseYI C'CCAG_C
Cuts at: 1426 2184 2761 3257 1426
Size: 758 577 496 2170
Fragments arranged by size:
2170 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1543 1589 2797 104
Size: 1439 46 1208 1308
Fragments arranged by size:
1439 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 975 1015 1793 1983 2775 975
Size: 40 778 190 792 2201
Fragments arranged by size:
2201 792 778 190 40
Bsp1286I G_dGCh'C
Cuts at: 594 975 1015 1626 1719 1793 1983 2045 2775
Size: 381 40 611 93 74 190 62 730
Cuts at: 2775 594
Size: 1820
Fragments arranged by size:
1820 730 611 381 190 93 74 62
40
BspEI T'CCGG_A
Cuts at: 3221 3221
Size: 4001
BspHI T'CATG_A
Cuts at: 3177 3177
Size: 4001
BspMI ACCTGCnnnn'nnnn_
Cuts at: 544 1051 1265 1567 1948 544
Size: 507 214 302 381 2597
Fragments arranged by size:
2597 507 381 302 214
BsrBI CCG'CTC
Cuts at: 50 981 2293 2347 3831 3996 50
Size: 931 1312 54 1484 165 55
Fragments arranged by size:
1484 1312 931 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1913 1913
Size: 4001
BsrFI r'CCGG_y
Cuts at: 1999 2180 2389 1999
Size: 181 209 3611
Fragments arranged by size:
3611 209 181
BssSI C'ACGA_G
Cuts at: 2272 2630 2272
Size: 358 3643
BstAPI GCAn_nnn'nTGC
Cuts at: 343 556 1378 343
Size: 213 822 2966
Fragments arranged by size:
2966 822 213
BstBI TT'CG_AA
Cuts at: 2362 2362
Size: 4001
BstYI r'GATC_y
Cuts at: 1 458 1026 1493 1516 1851 2097 3109 3195
Size: 457 568 467 23 335 246 1012 86
Cuts at: 3195 3207 3553 1
Size: 12 346 449
Fragments arranged by size:
1012 568 467 457 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4001
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1951 2132 2295 1951
Size: 181 163 3657
Fragments arranged by size:
3657 181 163
BtsI GCAGTG_nn'
Cuts at: 1244 1244
Size: 4001
DraI TTT'AAA
Cuts at: 1000 1000
Size: 4001
DrdI GACnn_nn'nnGTC
Cuts at: 1707 2565 1707
Size: 858 3143
EaeI y'GGCC_r
Cuts at: 1586 1760 2151 2178 3523 1586
Size: 174 391 27 1345 2064
Fragments arranged by size:
2064 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1586 1586
Size: 4001
EarI CTCTTCn'nnn_
Cuts at: 889 2024 2234 889
Size: 1135 210 2656
Fragments arranged by size:
2656 1135 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2209 2519 2665 3811 2209
Size: 310 146 1146 2399
Fragments arranged by size:
2399 1146 310 146
EcoICRI GAG'CTC
Cuts at: 973 1013 973
Size: 40 3961
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 616 788 1826 2258 3005 3273 616
Size: 172 1038 432 747 268 1344
Fragments arranged by size:
1344 1038 747 432 268 172
EcoO109I rG'GnC_Cy
Cuts at: 1140 1140
Size: 4001
EcoRI G'AATT_C
Cuts at: 1005 1005
Size: 4001
EcoRV GAT'ATC
Cuts at: 285 285
Size: 4001
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 159 978 2020 159
Size: 819 1042 2140
Fragments arranged by size:
2140 1042 819
FspI TGC'GCA
Cuts at: 336 1782 336
Size: 1446 2555
HaeII r_GCGC'y
Cuts at: 1435 1683 2705 3684 1435
Size: 248 1022 979 1752
Fragments arranged by size:
1752 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1843 1873 1875 1905 1843
Size: 30 2 30 3939
Fragments arranged by size:
3939 30 30 2
HincII GTy'rAC
Cuts at: 1040 1040
Size: 4001
HindIII A'AGCT_T
Cuts at: 960 960
Size: 4001
KasI G'GCGC_C
Cuts at: 1431 1679 1431
Size: 248 3753
KpnI G_GTAC'C
Cuts at: 1021 1021
Size: 4001
MluI A'CGCG_T
Cuts at: 3434 3434
Size: 4001
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2672 2856 2672
Size: 184 3817
MscI TGG'CCA
Cuts at: 1762 1762
Size: 4001
MslI CAynn'nnrTG
Cuts at: 1990 2117 1990
Size: 127 3874
MspA1I CmG'CkG
Cuts at: 1112 1426 1786 2799 3044 1112
Size: 314 360 1013 245 2069
Fragments arranged by size:
2069 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2182 2182
Size: 4001
NarI GG'CG_CC
Cuts at: 1432 1680 1432
Size: 248 3753
NcoI C'CATG_G
Cuts at: 107 107
Size: 4001
NgoMIV G'CCGG_C
Cuts at: 2180 2180
Size: 4001
NspI r_CATG'y
Cuts at: 732 1054 1554 2461 732
Size: 322 500 907 2272
Fragments arranged by size:
2272 907 500 322
PciI A'CATG_T
Cuts at: 2457 2457
Size: 4001
PflMI CCAn_nnn'nTGG
Cuts at: 364 364
Size: 4001
PmeI GTTT'AAAC
Cuts at: 1000 1000
Size: 4001
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3140 3172 3140
Size: 32 3969
PstI C_TGCA'G
Cuts at: 399 1048 1733 399
Size: 649 685 2667
Fragments arranged by size:
2667 685 649
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4001
PvuII CAG'CTG
Cuts at: 1426 1786 1426
Size: 360 3641
RsrII CG'GwC_CG
Cuts at: 2196 2196
Size: 4001
SacI G_AGCT'C
Cuts at: 975 1015 975
Size: 40 3961
SalI G'TCGA_C
Cuts at: 1038 1038
Size: 4001
SapI GCTCTTCn'nnn_
Cuts at: 2024 2234 2024
Size: 210 3791
SbfI CC_TGCA'GG
Cuts at: 1048 1048
Size: 4001
ScaI AGT'ACT
Cuts at: 3518 3518
Size: 4001
SfcI C'TryA_G
Cuts at: 33 395 1044 1729 2722 2913 33
Size: 362 649 685 993 191 1121
Fragments arranged by size:
1121 993 685 649 362 191
SfoI GGC'GCC
Cuts at: 1433 1681 1433
Size: 248 3753
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4001
SmaI CCC'GGG
Cuts at: 1023 1023
Size: 4001
SmlI C'TyrA_G
Cuts at: 1156 2071 2563 2825 1156
Size: 915 492 262 2332
Fragments arranged by size:
2332 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4001
SphI G_CATG'C
Cuts at: 1054 1554 1054
Size: 500 3501
StuI AGG'CCT
Cuts at: 139 554 139
Size: 415 3586
StyI C'CwwG_G
Cuts at: 107 1135 1489 107
Size: 1028 354 2619
Fragments arranged by size:
2619 1028 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 261 1584 1717 2176 2385 261
Size: 1323 133 459 209 1877
Fragments arranged by size:
1877 1323 459 209 133
TatI w'GTAC_w
Cuts at: 406 687 3516 406
Size: 281 2829 891
Fragments arranged by size:
2829 891 281
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3518 3550 3518
Size: 32 3969
Tth111I GACn'n_nGTC
Cuts at: 1798 1798
Size: 4001
XbaI T'CTAG_A
Cuts at: 1032 1032
Size: 4001
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1496 1496
Size: 4001
XmaI C'CCGG_G
Cuts at: 1021 1021
Size: 4001
XmnI GAAnn'nnTTC
Cuts at: 772 772
Size: 4001
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PflMI PmeI PpiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI FseI
FspAI HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PfoI PmlI PpuMI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI SwaI XhoI ZraI