Restriction map for
pF1KB3446
(Circular) (Six-base) MAPSORT of: pF1KB3446.seq Check: 551 from: 1 to: 3998
pF1KB3446 3998 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AccI GT'mk_AC
Cuts at: 1036 1036
Size: 3998
Acc65I G'GTAC_C
Cuts at: 1014 1014
Size: 3998
AclI AA'CG_TT
Cuts at: 3838 3838
Size: 3998
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 391 749 875 920 1823 2255 3002 3270 391
Size: 358 126 45 903 432 747 268 1119
Fragments arranged by size:
1119 903 747 432 358 268 126 45
AfeI AGC'GCT
Cuts at: 912 912
Size: 3998
AflII C'TTAA_G
Cuts at: 154 154
Size: 3998
AflIII A'CryG_T
Cuts at: 1350 2454 3431 1350
Size: 1104 977 1917
Fragments arranged by size:
1917 1104 977
AgeI A'CCGG_T
Cuts at: 2386 2386
Size: 3998
AleI CACnn'nnGTG
Cuts at: 182 182
Size: 3998
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 773 773
Size: 3998
AlwNI CAG_nnn'CTG
Cuts at: 1303 2870 1303
Size: 1567 2431
ApaLI G'TGCA_C
Cuts at: 2768 2768
Size: 3998
ApoI r'AATT_y
Cuts at: 784 1002 3351 3607 784
Size: 218 2349 256 1175
Fragments arranged by size:
2349 1175 256 218
AseI AT'TA_AT
Cuts at: 20 20
Size: 3998
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3998
AvaI C'yCGr_G
Cuts at: 644 1018 644
Size: 374 3624
BamHI G'GATC_C
Cuts at: 1023 1023
Size: 3998
BanI G'GyrC_C
Cuts at: 187 667 867 1014 1428 1676 1711 187
Size: 480 200 147 414 248 35 2474
Fragments arranged by size:
2474 480 414 248 200 147 35
BanII G_rGCy'C
Cuts at: 120 612 1012 2042 120
Size: 492 400 1030 2076
Fragments arranged by size:
2076 1030 492 400
BbeI G_GCGC'C
Cuts at: 1432 1680 1432
Size: 248 3750
BbsI GAAGACnn'nnnn_
Cuts at: 908 935 908
Size: 27 3971
BciVI GTATCCnnnnn_n'
Cuts at: 1896 2657 3998 1896
Size: 761 1341 1896
Fragments arranged by size:
1896 1341 761
BclI T'GATC_A
Cuts at: 1518 1518
Size: 3998
BglII A'GATC_T
Cuts at: 1513 3550 1513
Size: 2037 1961
BlpI GC'TnA_GC
Cuts at: 914 1110 914
Size: 196 3802
Bme1580I G_kGCm'C
Cuts at: 872 1623 1716 2772 872
Size: 751 93 1056 2098
Fragments arranged by size:
2098 1056 751 93
BmrI ACTGGGnnnn_n'
Cuts at: 1210 1291 1626 3770 1210
Size: 81 335 2144 1438
Fragments arranged by size:
2144 1438 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2365 2365
Size: 3998
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 327 410 927 327
Size: 83 517 3398
Fragments arranged by size:
3398 517 83
Bpu10I CC'TnA_GC
Cuts at: 953 953
Size: 3998
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 244 1174 2053 2545 2843 244
Size: 930 879 492 298 1399
Fragments arranged by size:
1399 930 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3702 3702
Size: 3998
BsaAI yAC'GTr
Cuts at: 1351 1351
Size: 3998
BsaBI GATnn'nnATC
Cuts at: 6 1512 3549 6
Size: 1506 2037 455
Fragments arranged by size:
2037 1506 455
BsaHI Gr'CG_yC
Cuts at: 1429 1677 2379 3875 1429
Size: 248 702 1496 1552
Fragments arranged by size:
1552 1496 702 248
BsaWI w'CCGG_w
Cuts at: 112 1187 1708 2386 2660 2807 3218 112
Size: 1075 521 678 274 147 411 892
Fragments arranged by size:
1075 892 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 546 576 546
Size: 30 3968
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 157 554 157
Size: 397 3601
BseYI C'CCAG_C
Cuts at: 1423 2181 2758 3254 1423
Size: 758 577 496 2167
Fragments arranged by size:
2167 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1540 1586 2794 104
Size: 1436 46 1208 1308
Fragments arranged by size:
1436 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 612 1012 1790 1980 2772 612
Size: 400 778 190 792 1838
Fragments arranged by size:
1838 792 778 400 190
Bsp1286I G_dGCh'C
Cuts at: 120 612 872 1012 1623 1716 1790 1980 2042
Size: 492 260 140 611 93 74 190 62
Cuts at: 2042 2772 120
Size: 730 1346
Fragments arranged by size:
1346 730 611 492 260 190 140 93
74 62
BspEI T'CCGG_A
Cuts at: 112 3218 112
Size: 3106 892
BspHI T'CATG_A
Cuts at: 3174 3174
Size: 3998
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1048 1262 1564 1945 1048
Size: 214 302 381 3101
Fragments arranged by size:
3101 381 302 214
BsrBI CCG'CTC
Cuts at: 50 811 2290 2344 3828 3993 50
Size: 761 1479 54 1484 165 55
Fragments arranged by size:
1484 1479 761 165 55 54
BsrDI GCAATG_nn'
Cuts at: 539 581 1910 539
Size: 42 1329 2627
Fragments arranged by size:
2627 1329 42
BsrFI r'CCGG_y
Cuts at: 1996 2177 2386 1996
Size: 181 209 3608
Fragments arranged by size:
3608 209 181
BsrGI T'GTAC_A
Cuts at: 861 861
Size: 3998
BssSI C'ACGA_G
Cuts at: 293 2269 2627 293
Size: 1976 358 1664
Fragments arranged by size:
1976 1664 358
BstAPI GCAn_nnn'nTGC
Cuts at: 295 304 1375 295
Size: 9 1071 2918
Fragments arranged by size:
2918 1071 9
BstBI TT'CG_AA
Cuts at: 2359 2359
Size: 3998
BstXI CCAn_nnnn'nTGG
Cuts at: 184 184
Size: 3998
BstYI r'GATC_y
Cuts at: 1 440 723 1023 1490 1513 1848 2094 3106
Size: 439 283 300 467 23 335 246 1012
Cuts at: 3106 3192 3204 3550 1
Size: 86 12 346 449
Fragments arranged by size:
1012 467 449 439 346 335 300 283
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 836 836
Size: 3998
BtgI C'CryG_G
Cuts at: 463 688 923 463
Size: 225 235 3538
Fragments arranged by size:
3538 235 225
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1948 2129 2292 1948
Size: 181 163 3654
Fragments arranged by size:
3654 181 163
BtsI GCAGTG_nn'
Cuts at: 776 821 947 1241 776
Size: 45 126 294 3533
Fragments arranged by size:
3533 294 126 45
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 384 419 384
Size: 35 3963
DraI TTT'AAA
Cuts at: 997 997
Size: 3998
DraIII CAC_nnn'GTG
Cuts at: 618 618
Size: 3998
DrdI GACnn_nn'nnGTC
Cuts at: 1704 2562 1704
Size: 858 3140
EaeI y'GGCC_r
Cuts at: 354 408 1583 1757 2148 2175 3520 354
Size: 54 1175 174 391 27 1345 832
Fragments arranged by size:
1345 1175 832 391 174 54 27
EagI C'GGCC_G
Cuts at: 1583 1583
Size: 3998
EarI CTCTTCn'nnn_
Cuts at: 725 740 2021 2231 725
Size: 15 1281 210 2492
Fragments arranged by size:
2492 1281 210 15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2206 2516 2662 3808 2206
Size: 310 146 1146 2396
Fragments arranged by size:
2396 1146 310 146
EcoICRI GAG'CTC
Cuts at: 610 1010 610
Size: 400 3598
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 327 391 410 749 875 920 927 1823 2255
Size: 64 19 339 126 45 7 896 432
Cuts at: 2255 3002 3270 327
Size: 747 268 1055
Fragments arranged by size:
1055 896 747 432 339 268 126 64
45 19 7
EcoO109I rG'GnC_Cy
Cuts at: 266 1137 266
Size: 871 3127
EcoRI G'AATT_C
Cuts at: 1002 1002
Size: 3998
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2017 2017
Size: 3998
FspI TGC'GCA
Cuts at: 658 1779 658
Size: 1121 2877
HaeII r_GCGC'y
Cuts at: 914 1432 1680 2702 3681 914
Size: 518 248 1022 979 1231
Fragments arranged by size:
1231 1022 979 518 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 627 659 1840 1870 1872 1902 627
Size: 32 1181 30 2 30 2723
Fragments arranged by size:
2723 1181 32 30 30 2
HincII GTy'rAC
Cuts at: 528 1037 528
Size: 509 3489
HindIII A'AGCT_T
Cuts at: 277 277
Size: 3998
HpaI GTT'AAC
Cuts at: 528 528
Size: 3998
KasI G'GCGC_C
Cuts at: 1428 1676 1428
Size: 248 3750
KpnI G_GTAC'C
Cuts at: 1018 1018
Size: 3998
MfeI C'AATT_G
Cuts at: 299 401 299
Size: 102 3896
MluI A'CGCG_T
Cuts at: 3431 3431
Size: 3998
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2669 2853 2669
Size: 184 3814
MscI TGG'CCA
Cuts at: 356 1759 356
Size: 1403 2595
MslI CAynn'nnrTG
Cuts at: 182 1987 2114 182
Size: 1805 127 2066
Fragments arranged by size:
2066 1805 127
MspA1I CmG'CkG
Cuts at: 1109 1423 1783 2796 3041 1109
Size: 314 360 1013 245 2066
Fragments arranged by size:
2066 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2179 2179
Size: 3998
NarI GG'CG_CC
Cuts at: 1429 1677 1429
Size: 248 3750
NcoI C'CATG_G
Cuts at: 688 688
Size: 3998
NgoMIV G'CCGG_C
Cuts at: 2177 2177
Size: 3998
NspI r_CATG'y
Cuts at: 663 1051 1551 2458 663
Size: 388 500 907 2203
Fragments arranged by size:
2203 907 500 388
PasI CC'CwG_GG
Cuts at: 488 488
Size: 3998
PciI A'CATG_T
Cuts at: 2454 2454
Size: 3998
PflMI CCAn_nnn'nTGG
Cuts at: 406 965 986 406
Size: 559 21 3418
Fragments arranged by size:
3418 559 21
PfoI T'CCnGG_A
Cuts at: 343 420 455 343
Size: 77 35 3886
Fragments arranged by size:
3886 77 35
PmeI GTTT'AAAC
Cuts at: 997 997
Size: 3998
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3137 3169 3137
Size: 32 3966
PpuMI rG'GwC_Cy
Cuts at: 266 266
Size: 3998
PstI C_TGCA'G
Cuts at: 942 1045 1730 942
Size: 103 685 3210
Fragments arranged by size:
3210 685 103
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3998
PvuII CAG'CTG
Cuts at: 1423 1783 1423
Size: 360 3638
RsrII CG'GwC_CG
Cuts at: 2193 2193
Size: 3998
SacI G_AGCT'C
Cuts at: 612 1012 612
Size: 400 3598
SalI G'TCGA_C
Cuts at: 1035 1035
Size: 3998
SapI GCTCTTCn'nnn_
Cuts at: 740 2021 2231 740
Size: 1281 210 2507
Fragments arranged by size:
2507 1281 210
SbfI CC_TGCA'GG
Cuts at: 1045 1045
Size: 3998
ScaI AGT'ACT
Cuts at: 3515 3515
Size: 3998
SfcI C'TryA_G
Cuts at: 33 717 938 1041 1726 2719 2910 33
Size: 684 221 103 685 993 191 1121
Fragments arranged by size:
1121 993 685 684 221 191 103
SfoI GGC'GCC
Cuts at: 1430 1678 1430
Size: 248 3750
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3998
SmaI CCC'GGG
Cuts at: 1020 1020
Size: 3998
SmlI C'TyrA_G
Cuts at: 154 259 1153 2068 2560 2822 154
Size: 105 894 915 492 262 1330
Fragments arranged by size:
1330 915 894 492 262 105
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3998
SphI G_CATG'C
Cuts at: 663 1051 1551 663
Size: 388 500 3110
Fragments arranged by size:
3110 500 388
StyI C'CwwG_G
Cuts at: 688 776 1132 1486 688
Size: 88 356 354 3200
Fragments arranged by size:
3200 356 354 88
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 701 1581 1714 2173 2382 701
Size: 880 133 459 209 2317
Fragments arranged by size:
2317 880 459 209 133
TatI w'GTAC_w
Cuts at: 215 710 861 3513 215
Size: 495 151 2652 700
Fragments arranged by size:
2652 700 495 151
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 546 578 3515 3547 546
Size: 32 2937 32 997
Fragments arranged by size:
2937 997 32 32
Tth111I GACn'n_nGTC
Cuts at: 1795 1795
Size: 3998
XbaI T'CTAG_A
Cuts at: 1029 1029
Size: 3998
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1493 1493
Size: 3998
XmaI C'CCGG_G
Cuts at: 1018 1018
Size: 3998
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI
AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI
BtgZI BtsI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AhdI AloI ApaI AscI AvrII BaeI
BbvCI BcgI BfrBI BglI BmgBI BmtI BsgI BsiWI
BsmI BsmBI BssHII BstEII BstZ17I ClaI EcoNI EcoRV
FseI FspAI NdeI NheI NotI NruI NsiI PacI
PmlI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI