Restriction map for
pF1KB3445
(Circular) (Six-base) MAPSORT of: pF1KB3445.seq Check: 2687 from: 1 to: 3719
pF1KB3445 3719 bp
With 182 enzymes: SgfI *
April 24, 2009 13:01 ..
AccI GT'mk_AC
Cuts at: 757 757
Size: 3719
Acc65I G'GTAC_C
Cuts at: 735 735
Size: 3719
AclI AA'CG_TT
Cuts at: 3559 3559
Size: 3719
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1544 1976 2723 2991 1544
Size: 432 747 268 2272
Fragments arranged by size:
2272 747 432 268
AflIII A'CryG_T
Cuts at: 1071 2175 3152 1071
Size: 1104 977 1638
Fragments arranged by size:
1638 1104 977
AgeI A'CCGG_T
Cuts at: 2107 2107
Size: 3719
AhdI GACnn_n'nnGTC
Cuts at: 335 381 335
Size: 46 3673
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 366 366
Size: 3719
AlwNI CAG_nnn'CTG
Cuts at: 1024 2591 1024
Size: 1567 2152
ApaLI G'TGCA_C
Cuts at: 2489 2489
Size: 3719
ApoI r'AATT_y
Cuts at: 723 3072 3328 723
Size: 2349 256 1114
Fragments arranged by size:
2349 1114 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3719
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3719
AvaI C'yCGr_G
Cuts at: 739 739
Size: 3719
BamHI G'GATC_C
Cuts at: 321 744 321
Size: 423 3296
BanI G'GyrC_C
Cuts at: 735 1149 1397 1432 735
Size: 414 248 35 3022
Fragments arranged by size:
3022 414 248 35
BanII G_rGCy'C
Cuts at: 430 548 733 1763 430
Size: 118 185 1030 2386
Fragments arranged by size:
2386 1030 185 118
BbeI G_GCGC'C
Cuts at: 1153 1401 1153
Size: 248 3471
BciVI GTATCCnnnnn_n'
Cuts at: 519 1617 2378 3719 519
Size: 1098 761 1341 519
Fragments arranged by size:
1341 1098 761 519
BclI T'GATC_A
Cuts at: 1239 1239
Size: 3719
BglII A'GATC_T
Cuts at: 1234 3271 1234
Size: 2037 1682
BlpI GC'TnA_GC
Cuts at: 831 831
Size: 3719
Bme1580I G_kGCm'C
Cuts at: 1344 1437 2493 1344
Size: 93 1056 2570
Fragments arranged by size:
2570 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 931 1012 1347 3491 931
Size: 81 335 2144 1159
Fragments arranged by size:
2144 1159 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2086 2086
Size: 3719
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 694 694
Size: 3719
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 895 1774 2266 2564 895
Size: 879 492 298 2050
Fragments arranged by size:
2050 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 322 3423 322
Size: 3101 618
BsaAI yAC'GTr
Cuts at: 1072 1072
Size: 3719
BsaBI GATnn'nnATC
Cuts at: 6 1233 3270 6
Size: 1227 2037 455
Fragments arranged by size:
2037 1227 455
BsaHI Gr'CG_yC
Cuts at: 1150 1398 2100 3596 1150
Size: 248 702 1496 1273
Fragments arranged by size:
1496 1273 702 248
BsaWI w'CCGG_w
Cuts at: 908 1429 2107 2381 2528 2939 908
Size: 521 678 274 147 411 1688
Fragments arranged by size:
1688 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 127 157 127
Size: 30 3689
BseYI C'CCAG_C
Cuts at: 1144 1902 2479 2975 1144
Size: 758 577 496 1888
Fragments arranged by size:
1888 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 464 464
Size: 3719
BsiEI CG_ry'CG
Cuts at: 104 1261 1307 2515 104
Size: 1157 46 1208 1308
Fragments arranged by size:
1308 1208 1157 46
BsiHKAI G_wGCw'C
Cuts at: 733 1511 1701 2493 733
Size: 778 190 792 1959
Fragments arranged by size:
1959 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 430 548 733 1344 1437 1511 1701 1763 2493
Size: 118 185 611 93 74 190 62 730
Cuts at: 2493 430
Size: 1656
Fragments arranged by size:
1656 730 611 190 185 118 93 74
62
BspEI T'CCGG_A
Cuts at: 2939 2939
Size: 3719
BspHI T'CATG_A
Cuts at: 2895 2895
Size: 3719
BspMI ACCTGCnnnn'nnnn_
Cuts at: 769 983 1285 1666 769
Size: 214 302 381 2822
Fragments arranged by size:
2822 381 302 214
BsrBI CCG'CTC
Cuts at: 50 138 242 2011 2065 3549 3714 50
Size: 88 104 1769 54 1484 165 55
Fragments arranged by size:
1769 1484 165 104 88 55 54
BsrDI GCAATG_nn'
Cuts at: 1631 1631
Size: 3719
BsrFI r'CCGG_y
Cuts at: 1717 1898 2107 1717
Size: 181 209 3329
Fragments arranged by size:
3329 209 181
BsrGI T'GTAC_A
Cuts at: 626 626
Size: 3719
BssSI C'ACGA_G
Cuts at: 1990 2348 1990
Size: 358 3361
BstAPI GCAn_nnn'nTGC
Cuts at: 1096 1096
Size: 3719
BstBI TT'CG_AA
Cuts at: 2080 2080
Size: 3719
BstEII G'GTnAC_C
Cuts at: 405 405
Size: 3719
BstYI r'GATC_y
Cuts at: 1 321 744 1211 1234 1569 1815 2827 2913
Size: 320 423 467 23 335 246 1012 86
Cuts at: 2913 2925 3271 1
Size: 12 346 449
Fragments arranged by size:
1012 467 449 423 346 335 320 246
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1669 1850 2013 1669
Size: 181 163 3375
Fragments arranged by size:
3375 181 163
BtsI GCAGTG_nn'
Cuts at: 962 962
Size: 3719
DraI TTT'AAA
Cuts at: 718 718
Size: 3719
DrdI GACnn_nn'nnGTC
Cuts at: 265 1425 2283 265
Size: 1160 858 1701
Fragments arranged by size:
1701 1160 858
EaeI y'GGCC_r
Cuts at: 455 467 1304 1478 1869 1896 3241 455
Size: 12 837 174 391 27 1345 933
Fragments arranged by size:
1345 933 837 391 174 27 12
EagI C'GGCC_G
Cuts at: 1304 1304
Size: 3719
EarI CTCTTCn'nnn_
Cuts at: 1742 1952 1742
Size: 210 3509
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1927 2237 2383 3529 1927
Size: 310 146 1146 2117
Fragments arranged by size:
2117 1146 310 146
EcoICRI GAG'CTC
Cuts at: 731 731
Size: 3719
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 694 1544 1976 2723 2991 694
Size: 850 432 747 268 1422
Fragments arranged by size:
1422 850 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 559 559
Size: 3719
EcoO109I rG'GnC_Cy
Cuts at: 569 858 569
Size: 289 3430
EcoRI G'AATT_C
Cuts at: 723 723
Size: 3719
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1738 1738
Size: 3719
FspI TGC'GCA
Cuts at: 1500 1500
Size: 3719
HaeII r_GCGC'y
Cuts at: 1153 1401 2423 3402 1153
Size: 248 1022 979 1470
Fragments arranged by size:
1470 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 111 143 321 353 367 399 589 621 1561
Size: 32 178 32 14 32 190 32 940
Cuts at: 1561 1591 1593 1623 111
Size: 30 2 30 2207
Fragments arranged by size:
2207 940 190 178 32 32 32 32
30 30 14 2
HincII GTy'rAC
Cuts at: 758 758
Size: 3719
KasI G'GCGC_C
Cuts at: 1149 1397 1149
Size: 248 3471
KpnI G_GTAC'C
Cuts at: 739 739
Size: 3719
MluI A'CGCG_T
Cuts at: 3152 3152
Size: 3719
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 175 592 2390 2574 175
Size: 417 1798 184 1320
Fragments arranged by size:
1798 1320 417 184
MscI TGG'CCA
Cuts at: 469 1480 469
Size: 1011 2708
MslI CAynn'nnrTG
Cuts at: 338 1708 1835 338
Size: 1370 127 2222
Fragments arranged by size:
2222 1370 127
MspA1I CmG'CkG
Cuts at: 830 1144 1504 2517 2762 830
Size: 314 360 1013 245 1787
Fragments arranged by size:
1787 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1900 1900
Size: 3719
NarI GG'CG_CC
Cuts at: 1150 1398 1150
Size: 248 3471
NgoMIV G'CCGG_C
Cuts at: 1898 1898
Size: 3719
NspI r_CATG'y
Cuts at: 772 1272 2179 772
Size: 500 907 2312
Fragments arranged by size:
2312 907 500
PciI A'CATG_T
Cuts at: 2175 2175
Size: 3719
PmeI GTTT'AAAC
Cuts at: 718 718
Size: 3719
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 170 202 2858 2890 170
Size: 32 2656 32 999
Fragments arranged by size:
2656 999 32 32
PstI C_TGCA'G
Cuts at: 553 766 1451 553
Size: 213 685 2821
Fragments arranged by size:
2821 685 213
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3719
PvuII CAG'CTG
Cuts at: 1144 1504 1144
Size: 360 3359
RsrII CG'GwC_CG
Cuts at: 1914 1914
Size: 3719
SacI G_AGCT'C
Cuts at: 733 733
Size: 3719
SalI G'TCGA_C
Cuts at: 756 756
Size: 3719
SapI GCTCTTCn'nnn_
Cuts at: 1742 1952 1742
Size: 210 3509
SbfI CC_TGCA'GG
Cuts at: 766 766
Size: 3719
ScaI AGT'ACT
Cuts at: 3236 3236
Size: 3719
SexAI A'CCwGG_T
Cuts at: 409 409
Size: 3719
SfcI C'TryA_G
Cuts at: 33 549 762 1447 2440 2631 33
Size: 516 213 685 993 191 1121
Fragments arranged by size:
1121 993 685 516 213 191
SfoI GGC'GCC
Cuts at: 1151 1399 1151
Size: 248 3471
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3719
SmaI CCC'GGG
Cuts at: 741 741
Size: 3719
SmlI C'TyrA_G
Cuts at: 874 1789 2281 2543 874
Size: 915 492 262 2050
Fragments arranged by size:
2050 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3719
SphI G_CATG'C
Cuts at: 772 1272 772
Size: 500 3219
StyI C'CwwG_G
Cuts at: 652 853 1207 652
Size: 201 354 3164
Fragments arranged by size:
3164 354 201
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1302 1435 1894 2103 1302
Size: 133 459 209 2918
Fragments arranged by size:
2918 459 209 133
TatI w'GTAC_w
Cuts at: 626 3234 626
Size: 2608 1111
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3236 3268 3236
Size: 32 3687
Tth111I GACn'n_nGTC
Cuts at: 1516 1516
Size: 3719
XbaI T'CTAG_A
Cuts at: 750 750
Size: 3719
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 650 1214 650
Size: 564 3155
XmaI C'CCGG_G
Cuts at: 739 739
Size: 3719
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstEII BstYI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PciI
PmeI PpiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AloI ApaI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BglI BmgBI
BmtI Bpu10I BseRI BsiWI BsmI BsmBI BssHII BstXI
BstZ17I Bsu36I BtgI ClaI CspCI DraIII EcoRV FseI
FspAI HindIII HpaI MfeI NcoI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PfoI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI