Restriction map for
pF1KB3435
(Circular) (Six-base) MAPSORT of: pF1KB3435.seq Check: 9959 from: 1 to: 4331
pF1KB3435 4331 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AccI GT'mk_AC
Cuts at: 1369 1369
Size: 4331
Acc65I G'GTAC_C
Cuts at: 577 1347 577
Size: 770 3561
AclI AA'CG_TT
Cuts at: 4171 4171
Size: 4331
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2156 2588 3335 3603 2156
Size: 432 747 268 2884
Fragments arranged by size:
2884 747 432 268
AflIII A'CryG_T
Cuts at: 1683 2787 3764 1683
Size: 1104 977 2250
Fragments arranged by size:
2250 1104 977
AgeI A'CCGG_T
Cuts at: 2719 2719
Size: 4331
AleI CACnn'nnGTG
Cuts at: 523 523
Size: 4331
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 541 1135 541
Size: 594 3737
AlwNI CAG_nnn'CTG
Cuts at: 546 1636 3203 546
Size: 1090 1567 1674
Fragments arranged by size:
1674 1567 1090
ApaLI G'TGCA_C
Cuts at: 3101 3101
Size: 4331
ApoI r'AATT_y
Cuts at: 1335 3684 3940 1335
Size: 2349 256 1726
Fragments arranged by size:
2349 1726 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4331
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4331
AvaI C'yCGr_G
Cuts at: 434 989 1351 434
Size: 555 362 3414
Fragments arranged by size:
3414 555 362
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 492 525 492
Size: 33 4298
BamHI G'GATC_C
Cuts at: 1356 1356
Size: 4331
BanI G'GyrC_C
Cuts at: 490 517 577 1347 1761 2009 2044 490
Size: 27 60 770 414 248 35 2777
Fragments arranged by size:
2777 770 414 248 60 35 27
BanII G_rGCy'C
Cuts at: 164 1345 2375 164
Size: 1181 1030 2120
Fragments arranged by size:
2120 1181 1030
BbeI G_GCGC'C
Cuts at: 494 1765 2013 494
Size: 1271 248 2812
Fragments arranged by size:
2812 1271 248
BbsI GAAGACnn'nnnn_
Cuts at: 1250 1250
Size: 4331
BbvCI CC'TCA_GC
Cuts at: 533 533
Size: 4331
BciVI GTATCCnnnnn_n'
Cuts at: 853 1014 2229 2990 4331 853
Size: 161 1215 761 1341 853
Fragments arranged by size:
1341 1215 853 761 161
BclI T'GATC_A
Cuts at: 1851 1851
Size: 4331
BfrBI ATG'CAT
Cuts at: 984 1014 984
Size: 30 4301
BglI GCCn_nnn'nGGC
Cuts at: 412 412
Size: 4331
BglII A'GATC_T
Cuts at: 396 692 1846 3883 396
Size: 296 1154 2037 844
Fragments arranged by size:
2037 1154 844 296
BlpI GC'TnA_GC
Cuts at: 446 1443 446
Size: 997 3334
Bme1580I G_kGCm'C
Cuts at: 520 1956 2049 3105 520
Size: 1436 93 1056 1746
Fragments arranged by size:
1746 1436 1056 93
BmgBI CAC'GTC
Cuts at: 1044 1044
Size: 4331
BmrI ACTGGGnnnn_n'
Cuts at: 356 1543 1624 1959 4103 356
Size: 1187 81 335 2144 584
Fragments arranged by size:
2144 1187 584 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 288 2698 288
Size: 2410 1921
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 856 856
Size: 4331
Bpu10I CC'TnA_GC
Cuts at: 533 533
Size: 4331
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 314 790 1507 2386 2878 3176 314
Size: 476 717 879 492 298 1469
Fragments arranged by size:
1469 879 717 492 476 298
BsaI GGTCTCn'nnnn_
Cuts at: 1264 4035 1264
Size: 2771 1560
BsaAI yAC'GTr
Cuts at: 1684 1684
Size: 4331
BsaBI GATnn'nnATC
Cuts at: 6 1845 3882 6
Size: 1839 2037 455
Fragments arranged by size:
2037 1839 455
BsaHI Gr'CG_yC
Cuts at: 170 491 1762 2010 2712 4208 170
Size: 321 1271 248 702 1496 293
Fragments arranged by size:
1496 1271 702 321 293 248
BsaWI w'CCGG_w
Cuts at: 608 1520 2041 2719 2993 3140 3551 608
Size: 912 521 678 274 147 411 1388
Fragments arranged by size:
1388 912 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1270 1300 1270
Size: 30 4301
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 889 889
Size: 4331
BseYI C'CCAG_C
Cuts at: 1756 2514 3091 3587 1756
Size: 758 577 496 2500
Fragments arranged by size:
2500 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 559 1141 559
Size: 582 3749
BsiEI CG_ry'CG
Cuts at: 104 1191 1873 1919 3127 104
Size: 1087 682 46 1208 1308
Fragments arranged by size:
1308 1208 1087 682 46
BsiHKAI G_wGCw'C
Cuts at: 1345 2123 2313 3105 1345
Size: 778 190 792 2571
Fragments arranged by size:
2571 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 1034 1034
Size: 4331
Bsp1286I G_dGCh'C
Cuts at: 164 520 1345 1956 2049 2123 2313 2375 3105
Size: 356 825 611 93 74 190 62 730
Cuts at: 3105 164
Size: 1390
Fragments arranged by size:
1390 825 730 611 356 190 93 74
62
BspEI T'CCGG_A
Cuts at: 3551 3551
Size: 4331
BspHI T'CATG_A
Cuts at: 788 3507 788
Size: 2719 1612
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1106 1116 1381 1595 1897 2278 1106
Size: 10 265 214 302 381 3159
Fragments arranged by size:
3159 381 302 265 214 10
BsrBI CCG'CTC
Cuts at: 50 2623 2677 4161 4326 50
Size: 2573 54 1484 165 55
Fragments arranged by size:
2573 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2243 2243
Size: 4331
BsrFI r'CCGG_y
Cuts at: 2329 2510 2719 2329
Size: 181 209 3941
Fragments arranged by size:
3941 209 181
BssSI C'ACGA_G
Cuts at: 2602 2960 2602
Size: 358 3973
BstAPI GCAn_nnn'nTGC
Cuts at: 546 1708 546
Size: 1162 3169
BstBI TT'CG_AA
Cuts at: 2692 2692
Size: 4331
BstEII G'GTnAC_C
Cuts at: 774 774
Size: 4331
BstYI r'GATC_y
Cuts at: 1 396 692 1356 1823 1846 2181 2427 3439
Size: 395 296 664 467 23 335 246 1012
Cuts at: 3439 3525 3537 3883 1
Size: 86 12 346 449
Fragments arranged by size:
1012 664 467 449 395 346 335 296
246 86 23 12
BtgI C'CryG_G
Cuts at: 240 675 240
Size: 435 3896
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2281 2462 2625 2281
Size: 181 163 3987
Fragments arranged by size:
3987 181 163
BtsI GCAGTG_nn'
Cuts at: 1574 1574
Size: 4331
ClaI AT'CG_AT
Cuts at: 373 373
Size: 4331
DraI TTT'AAA
Cuts at: 1330 1330
Size: 4331
DraIII CAC_nnn'GTG
Cuts at: 898 898
Size: 4331
DrdI GACnn_nn'nnGTC
Cuts at: 2037 2895 2037
Size: 858 3473
EaeI y'GGCC_r
Cuts at: 356 585 1103 1206 1916 2090 2481 2508 3853
Size: 229 518 103 710 174 391 27 1345
Cuts at: 3853 356
Size: 834
Fragments arranged by size:
1345 834 710 518 391 229 174 103
27
EagI C'GGCC_G
Cuts at: 1916 1916
Size: 4331
EarI CTCTTCn'nnn_
Cuts at: 818 1262 2354 2564 818
Size: 444 1092 210 2585
Fragments arranged by size:
2585 1092 444 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 835 996 2539 2849 2995 4141 835
Size: 161 1543 310 146 1146 1025
Fragments arranged by size:
1543 1146 1025 310 161 146
EcoICRI GAG'CTC
Cuts at: 1343 1343
Size: 4331
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 856 2156 2588 3335 3603 856
Size: 1300 432 747 268 1584
Fragments arranged by size:
1584 1300 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 795 1470 795
Size: 675 3656
EcoRI G'AATT_C
Cuts at: 1335 1335
Size: 4331
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2350 2350
Size: 4331
FspI TGC'GCA
Cuts at: 2112 2112
Size: 4331
HaeII r_GCGC'y
Cuts at: 494 1765 2013 3035 4014 494
Size: 1271 248 1022 979 811
Fragments arranged by size:
1271 1022 979 811 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 115 147 543 575 972 1004 2173 2203 2205
Size: 32 396 32 397 32 1169 30 2
Cuts at: 2205 2235 115
Size: 30 2211
Fragments arranged by size:
2211 1169 397 396 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 201 830 1370 201
Size: 629 540 3162
Fragments arranged by size:
3162 629 540
KasI G'GCGC_C
Cuts at: 490 1761 2009 490
Size: 1271 248 2812
Fragments arranged by size:
2812 1271 248
KpnI G_GTAC'C
Cuts at: 581 1351 581
Size: 770 3561
MluI A'CGCG_T
Cuts at: 3764 3764
Size: 4331
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3002 3186 3002
Size: 184 4147
MscI TGG'CCA
Cuts at: 1105 2092 1105
Size: 987 3344
MslI CAynn'nnrTG
Cuts at: 523 571 637 862 1117 2320 2447 523
Size: 48 66 225 255 1203 127 2407
Fragments arranged by size:
2407 1203 255 225 127 66 48
MspA1I CmG'CkG
Cuts at: 1442 1756 2116 3129 3374 1442
Size: 314 360 1013 245 2399
Fragments arranged by size:
2399 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2512 2512
Size: 4331
NarI GG'CG_CC
Cuts at: 491 1762 2010 491
Size: 1271 248 2812
Fragments arranged by size:
2812 1271 248
NgoMIV G'CCGG_C
Cuts at: 2510 2510
Size: 4331
NsiI A_TGCA'T
Cuts at: 986 1016 986
Size: 30 4301
NspI r_CATG'y
Cuts at: 988 1384 1884 2791 988
Size: 396 500 907 2528
Fragments arranged by size:
2528 907 500 396
PciI A'CATG_T
Cuts at: 2787 2787
Size: 4331
PflMI CCAn_nnn'nTGG
Cuts at: 417 417
Size: 4331
PmeI GTTT'AAAC
Cuts at: 1330 1330
Size: 4331
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3470 3502 3470
Size: 32 4299
PpuMI rG'GwC_Cy
Cuts at: 795 795
Size: 4331
PstI C_TGCA'G
Cuts at: 383 551 1378 2063 383
Size: 168 827 685 2651
Fragments arranged by size:
2651 827 685 168
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4331
PvuII CAG'CTG
Cuts at: 1756 2116 1756
Size: 360 3971
RsrII CG'GwC_CG
Cuts at: 2526 2526
Size: 4331
SacI G_AGCT'C
Cuts at: 1345 1345
Size: 4331
SalI G'TCGA_C
Cuts at: 1368 1368
Size: 4331
SanDI GG'GwC_CC
Cuts at: 795 795
Size: 4331
SapI GCTCTTCn'nnn_
Cuts at: 2354 2564 2354
Size: 210 4121
SbfI CC_TGCA'GG
Cuts at: 1378 1378
Size: 4331
ScaI AGT'ACT
Cuts at: 3848 3848
Size: 4331
SfcI C'TryA_G
Cuts at: 33 379 547 1374 2059 3052 3243 33
Size: 346 168 827 685 993 191 1121
Fragments arranged by size:
1121 993 827 685 346 191 168
SfoI GGC'GCC
Cuts at: 492 1763 2011 492
Size: 1271 248 2812
Fragments arranged by size:
2812 1271 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4331
SmaI CCC'GGG
Cuts at: 1353 1353
Size: 4331
SmlI C'TyrA_G
Cuts at: 329 434 769 989 1486 2401 2893 3155 329
Size: 105 335 220 497 915 492 262 1505
Fragments arranged by size:
1505 915 497 492 335 262 220 105
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4331
SphI G_CATG'C
Cuts at: 988 1384 1884 988
Size: 396 500 3435
Fragments arranged by size:
3435 500 396
StuI AGG'CCT
Cuts at: 415 415
Size: 4331
StyI C'CwwG_G
Cuts at: 416 1465 1819 416
Size: 1049 354 2928
Fragments arranged by size:
2928 1049 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 424 444 742 940 1177 1914 2047 2506 2715
Size: 20 298 198 237 737 133 459 209
Cuts at: 2715 424
Size: 2040
Fragments arranged by size:
2040 737 459 298 237 209 198 133
20
TatI w'GTAC_w
Cuts at: 645 3846 645
Size: 3201 1130
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3848 3880 3848
Size: 32 4299
Tth111I GACn'n_nGTC
Cuts at: 2128 2128
Size: 4331
XbaI T'CTAG_A
Cuts at: 1362 1362
Size: 4331
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 343 414 438 1098 1826 343
Size: 71 24 660 728 2848
Fragments arranged by size:
2848 728 660 71 24
XhoI C'TCGA_G
Cuts at: 434 989 434
Size: 555 3776
XmaI C'CCGG_G
Cuts at: 1351 1351
Size: 4331
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstEII BstYI BtgI BtgZI BtsI ClaI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NsiI NspI PciI PflMI PmeI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI ApaI AscI
AvrII BcgI BmtI BsiWI BsmI BsrGI BssHII BstXI
BstZ17I Bsu36I CspCI EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NcoI NdeI NheI NotI NruI PacI
PasI PfoI PmlI PshAI PsiI PspOMI PspXI PsrI
SacII SexAI SfiI SgrAI SnaBI SrfI SspI SwaI
XmnI ZraI