Restriction map for
pF1KB3431
(Circular) (Six-base) MAPSORT of: pF1KB3431.seq Check: 1990 from: 1 to: 3788
pF1KB3431 3788 bp
With 182 enzymes: SgfI *
April 24, 2009 13:01 ..
AccI GT'mk_AC
Cuts at: 826 826
Size: 3788
Acc65I G'GTAC_C
Cuts at: 804 804
Size: 3788
AclI AA'CG_TT
Cuts at: 3628 3628
Size: 3788
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1613 2045 2792 3060 1613
Size: 432 747 268 2341
Fragments arranged by size:
2341 747 432 268
AflIII A'CryG_T
Cuts at: 1140 2244 3221 1140
Size: 1104 977 1707
Fragments arranged by size:
1707 1104 977
AgeI A'CCGG_T
Cuts at: 2176 2176
Size: 3788
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 746 746
Size: 3788
AlwNI CAG_nnn'CTG
Cuts at: 1093 2660 1093
Size: 1567 2221
ApaLI G'TGCA_C
Cuts at: 2558 2558
Size: 3788
ApoI r'AATT_y
Cuts at: 792 3141 3397 792
Size: 2349 256 1183
Fragments arranged by size:
2349 1183 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3788
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3788
AvaI C'yCGr_G
Cuts at: 808 808
Size: 3788
BamHI G'GATC_C
Cuts at: 813 813
Size: 3788
BanI G'GyrC_C
Cuts at: 804 1218 1466 1501 804
Size: 414 248 35 3091
Fragments arranged by size:
3091 414 248 35
BanII G_rGCy'C
Cuts at: 628 802 1832 628
Size: 174 1030 2584
Fragments arranged by size:
2584 1030 174
BbeI G_GCGC'C
Cuts at: 1222 1470 1222
Size: 248 3540
BbsI GAAGACnn'nnnn_
Cuts at: 368 518 368
Size: 150 3638
BciVI GTATCCnnnnn_n'
Cuts at: 643 1686 2447 3788 643
Size: 1043 761 1341 643
Fragments arranged by size:
1341 1043 761 643
BclI T'GATC_A
Cuts at: 1308 1308
Size: 3788
BglI GCCn_nnn'nGGC
Cuts at: 137 137
Size: 3788
BglII A'GATC_T
Cuts at: 1303 3340 1303
Size: 2037 1751
BlpI GC'TnA_GC
Cuts at: 900 900
Size: 3788
Bme1580I G_kGCm'C
Cuts at: 1413 1506 2562 1413
Size: 93 1056 2639
Fragments arranged by size:
2639 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1000 1081 1416 3560 1000
Size: 81 335 2144 1228
Fragments arranged by size:
2144 1228 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2155 2155
Size: 3788
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 127 736 127
Size: 609 3179
Bpu10I CC'TnA_GC
Cuts at: 460 460
Size: 3788
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 964 1843 2335 2633 964
Size: 879 492 298 2119
Fragments arranged by size:
2119 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 113 3492 113
Size: 3379 409
BsaAI yAC'GTr
Cuts at: 1141 1141
Size: 3788
BsaBI GATnn'nnATC
Cuts at: 6 1302 3339 6
Size: 1296 2037 455
Fragments arranged by size:
2037 1296 455
BsaHI Gr'CG_yC
Cuts at: 1219 1467 2169 3665 1219
Size: 248 702 1496 1342
Fragments arranged by size:
1496 1342 702 248
BsaWI w'CCGG_w
Cuts at: 977 1498 2176 2450 2597 3008 977
Size: 521 678 274 147 411 1757
Fragments arranged by size:
1757 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 448 598 733 448
Size: 150 135 3503
Fragments arranged by size:
3503 150 135
BseYI C'CCAG_C
Cuts at: 1213 1971 2548 3044 1213
Size: 758 577 496 1957
Fragments arranged by size:
1957 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 494 769 494
Size: 275 3513
BsiEI CG_ry'CG
Cuts at: 104 1330 1376 2584 104
Size: 1226 46 1208 1308
Fragments arranged by size:
1308 1226 1208 46
BsiHKAI G_wGCw'C
Cuts at: 152 802 1580 1770 2562 152
Size: 650 778 190 792 1378
Fragments arranged by size:
1378 792 778 650 190
BsmI GAATG_Cn'
Cuts at: 599 599
Size: 3788
Bsp1286I G_dGCh'C
Cuts at: 152 628 802 1413 1506 1580 1770 1832 2562
Size: 476 174 611 93 74 190 62 730
Cuts at: 2562 152
Size: 1378
Fragments arranged by size:
1378 730 611 476 190 174 93 74
62
BspEI T'CCGG_A
Cuts at: 3008 3008
Size: 3788
BspHI T'CATG_A
Cuts at: 2964 2964
Size: 3788
BspMI ACCTGCnnnn'nnnn_
Cuts at: 516 838 1052 1354 1735 516
Size: 322 214 302 381 2569
Fragments arranged by size:
2569 381 322 302 214
BsrBI CCG'CTC
Cuts at: 50 2080 2134 3618 3783 50
Size: 2030 54 1484 165 55
Fragments arranged by size:
2030 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1700 1700
Size: 3788
BsrFI r'CCGG_y
Cuts at: 136 1786 1967 2176 136
Size: 1650 181 209 1748
Fragments arranged by size:
1748 1650 209 181
BssSI C'ACGA_G
Cuts at: 2059 2417 2059
Size: 358 3430
BstAPI GCAn_nnn'nTGC
Cuts at: 517 1165 517
Size: 648 3140
BstBI TT'CG_AA
Cuts at: 2149 2149
Size: 3788
BstXI CCAn_nnnn'nTGG
Cuts at: 762 762
Size: 3788
BstYI r'GATC_y
Cuts at: 1 421 813 1280 1303 1638 1884 2896 2982
Size: 420 392 467 23 335 246 1012 86
Cuts at: 2982 2994 3340 1
Size: 12 346 449
Fragments arranged by size:
1012 467 449 420 392 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 755 107
Size: 648 3140
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1738 1919 2082 1738
Size: 181 163 3444
Fragments arranged by size:
3444 181 163
BtsI GCAGTG_nn'
Cuts at: 728 1031 728
Size: 303 3485
DraI TTT'AAA
Cuts at: 787 787
Size: 3788
DrdI GACnn_nn'nnGTC
Cuts at: 616 1494 2352 616
Size: 878 858 2052
Fragments arranged by size:
2052 878 858
EaeI y'GGCC_r
Cuts at: 1373 1547 1938 1965 3310 1373
Size: 174 391 27 1345 1851
Fragments arranged by size:
1851 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1373 1373
Size: 3788
EarI CTCTTCn'nnn_
Cuts at: 1811 2021 1811
Size: 210 3578
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 181 1996 2306 2452 3598 181
Size: 1815 310 146 1146 371
Fragments arranged by size:
1815 1146 371 310 146
EcoICRI GAG'CTC
Cuts at: 800 800
Size: 3788
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 127 736 1613 2045 2792 3060 127
Size: 609 877 432 747 268 855
Fragments arranged by size:
877 855 747 609 432 268
EcoO109I rG'GnC_Cy
Cuts at: 927 927
Size: 3788
EcoRI G'AATT_C
Cuts at: 792 792
Size: 3788
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 490 1807 490
Size: 1317 2471
FspI TGC'GCA
Cuts at: 1569 1569
Size: 3788
HaeII r_GCGC'y
Cuts at: 1222 1470 2492 3471 1222
Size: 248 1022 979 1539
Fragments arranged by size:
1539 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1630 1660 1662 1692 1630
Size: 30 2 30 3726
Fragments arranged by size:
3726 30 30 2
HincII GTy'rAC
Cuts at: 827 827
Size: 3788
HindIII A'AGCT_T
Cuts at: 329 329
Size: 3788
KasI G'GCGC_C
Cuts at: 1218 1466 1218
Size: 248 3540
KpnI G_GTAC'C
Cuts at: 808 808
Size: 3788
MluI A'CGCG_T
Cuts at: 3221 3221
Size: 3788
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 681 2459 2643 681
Size: 1778 184 1826
Fragments arranged by size:
1826 1778 184
MscI TGG'CCA
Cuts at: 1549 1549
Size: 3788
MslI CAynn'nnrTG
Cuts at: 760 1777 1904 760
Size: 1017 127 2644
Fragments arranged by size:
2644 1017 127
MspA1I CmG'CkG
Cuts at: 899 1213 1573 2586 2831 899
Size: 314 360 1013 245 1856
Fragments arranged by size:
1856 1013 360 314 245
NaeI GCC'GGC
Cuts at: 138 1969 138
Size: 1831 1957
NarI GG'CG_CC
Cuts at: 1219 1467 1219
Size: 248 3540
NcoI C'CATG_G
Cuts at: 107 755 107
Size: 648 3140
NgoMIV G'CCGG_C
Cuts at: 136 1967 136
Size: 1831 1957
NspI r_CATG'y
Cuts at: 841 1341 2248 841
Size: 500 907 2381
Fragments arranged by size:
2381 907 500
PciI A'CATG_T
Cuts at: 2244 2244
Size: 3788
PmeI GTTT'AAAC
Cuts at: 787 787
Size: 3788
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2927 2959 2927
Size: 32 3756
PshAI GACnn'nnGTC
Cuts at: 254 254
Size: 3788
PstI C_TGCA'G
Cuts at: 835 1520 835
Size: 685 3103
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3788
PvuII CAG'CTG
Cuts at: 1213 1573 1213
Size: 360 3428
RsrII CG'GwC_CG
Cuts at: 1983 1983
Size: 3788
SacI G_AGCT'C
Cuts at: 802 802
Size: 3788
SalI G'TCGA_C
Cuts at: 825 825
Size: 3788
SapI GCTCTTCn'nnn_
Cuts at: 1811 2021 1811
Size: 210 3578
SbfI CC_TGCA'GG
Cuts at: 835 835
Size: 3788
ScaI AGT'ACT
Cuts at: 3305 3305
Size: 3788
SfcI C'TryA_G
Cuts at: 33 368 383 831 1516 2509 2700 33
Size: 335 15 448 685 993 191 1121
Fragments arranged by size:
1121 993 685 448 335 191 15
SfoI GGC'GCC
Cuts at: 1220 1468 1220
Size: 248 3540
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3788
SmaI CCC'GGG
Cuts at: 810 810
Size: 3788
SmlI C'TyrA_G
Cuts at: 943 1858 2350 2612 943
Size: 915 492 262 2119
Fragments arranged by size:
2119 915 492 262
SpeI A'CTAG_T
Cuts at: 67 636 67
Size: 569 3219
SphI G_CATG'C
Cuts at: 841 1341 841
Size: 500 3288
StyI C'CwwG_G
Cuts at: 107 550 755 922 1276 107
Size: 443 205 167 354 2619
Fragments arranged by size:
2619 443 354 205 167
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1371 1504 1963 2172 1371
Size: 133 459 209 2987
Fragments arranged by size:
2987 459 209 133
TatI w'GTAC_w
Cuts at: 3303 3303
Size: 3788
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3305 3337 3305
Size: 32 3756
Tth111I GACn'n_nGTC
Cuts at: 1585 1585
Size: 3788
XbaI T'CTAG_A
Cuts at: 819 819
Size: 3788
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1283 1283
Size: 3788
XmaI C'CCGG_G
Cuts at: 808 808
Size: 3788
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PmeI PpiI PshAI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI
BsaXI BsiWI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PfoI PmlI PpuMI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI