Restriction map for
pF1KB3423
(Circular) (Six-base) MAPSORT of: pF1KB3423.seq Check: 2937 from: 1 to: 4115
pF1KB3423 4115 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 696 768 696
Size: 72 4043
AccI GT'mk_AC
Cuts at: 1153 1153
Size: 4115
Acc65I G'GTAC_C
Cuts at: 1131 1131
Size: 4115
AclI AA'CG_TT
Cuts at: 3955 3955
Size: 4115
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1940 2372 3119 3387 1940
Size: 432 747 268 2668
Fragments arranged by size:
2668 747 432 268
AflIII A'CryG_T
Cuts at: 1467 2571 3548 1467
Size: 1104 977 2034
Fragments arranged by size:
2034 1104 977
AgeI A'CCGG_T
Cuts at: 2503 2503
Size: 4115
AleI CACnn'nnGTG
Cuts at: 676 676
Size: 4115
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 854 854
Size: 4115
AlwNI CAG_nnn'CTG
Cuts at: 1420 2987 1420
Size: 1567 2548
ApaLI G'TGCA_C
Cuts at: 2885 2885
Size: 4115
ApoI r'AATT_y
Cuts at: 201 427 1119 3468 3724 201
Size: 226 692 2349 256 592
Fragments arranged by size:
2349 692 592 256 226
AseI AT'TA_AT
Cuts at: 20 20
Size: 4115
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4115
AvaI C'yCGr_G
Cuts at: 179 1135 179
Size: 956 3159
BamHI G'GATC_C
Cuts at: 1140 1140
Size: 4115
BanI G'GyrC_C
Cuts at: 1131 1545 1793 1828 1131
Size: 414 248 35 3418
Fragments arranged by size:
3418 414 248 35
BanII G_rGCy'C
Cuts at: 1129 2159 1129
Size: 1030 3085
BbeI G_GCGC'C
Cuts at: 1549 1797 1549
Size: 248 3867
BbvCI CC'TCA_GC
Cuts at: 656 656
Size: 4115
BciVI GTATCCnnnnn_n'
Cuts at: 2013 2774 4115 2013
Size: 761 1341 2013
Fragments arranged by size:
2013 1341 761
BclI T'GATC_A
Cuts at: 287 432 1635 287
Size: 145 1203 2767
Fragments arranged by size:
2767 1203 145
BglI GCCn_nnn'nGGC
Cuts at: 748 748
Size: 4115
BglII A'GATC_T
Cuts at: 1630 3667 1630
Size: 2037 2078
BlpI GC'TnA_GC
Cuts at: 554 725 864 1227 554
Size: 171 139 363 3442
Fragments arranged by size:
3442 363 171 139
Bme1580I G_kGCm'C
Cuts at: 1740 1833 2889 1740
Size: 93 1056 2966
Fragments arranged by size:
2966 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1327 1408 1743 3887 1327
Size: 81 335 2144 1555
Fragments arranged by size:
2144 1555 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2482 2482
Size: 4115
Bpu10I CC'TnA_GC
Cuts at: 656 656
Size: 4115
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 872 1291 2170 2662 2960 872
Size: 419 879 492 298 2027
Fragments arranged by size:
2027 879 492 419 298
BsaI GGTCTCn'nnnn_
Cuts at: 3819 3819
Size: 4115
BsaAI yAC'GTr
Cuts at: 1468 1468
Size: 4115
BsaBI GATnn'nnATC
Cuts at: 6 334 643 1629 3666 6
Size: 328 309 986 2037 455
Fragments arranged by size:
2037 986 455 328 309
BsaHI Gr'CG_yC
Cuts at: 1546 1794 2496 3992 1546
Size: 248 702 1496 1669
Fragments arranged by size:
1669 1496 702 248
BsaWI w'CCGG_w
Cuts at: 197 1304 1825 2503 2777 2924 3335 197
Size: 1107 521 678 274 147 411 977
Fragments arranged by size:
1107 977 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1060 1090 1060
Size: 30 4085
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 511 511
Size: 4115
BseYI C'CCAG_C
Cuts at: 152 869 1043 1540 2298 2875 3371 152
Size: 717 174 497 758 577 496 896
Fragments arranged by size:
896 758 717 577 497 496 174
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 247 314 730 850 916 247
Size: 67 416 120 66 3446
Fragments arranged by size:
3446 416 120 67 66
BsiEI CG_ry'CG
Cuts at: 104 1657 1703 2911 104
Size: 1553 46 1208 1308
Fragments arranged by size:
1553 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1129 1907 2097 2889 1129
Size: 778 190 792 2355
Fragments arranged by size:
2355 792 778 190
BsmI GAATG_Cn'
Cuts at: 843 843
Size: 4115
Bsp1286I G_dGCh'C
Cuts at: 1129 1740 1833 1907 2097 2159 2889 1129
Size: 611 93 74 190 62 730 2355
Fragments arranged by size:
2355 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 197 3335 197
Size: 3138 977
BspHI T'CATG_A
Cuts at: 3291 3291
Size: 4115
BspMI ACCTGCnnnn'nnnn_
Cuts at: 678 696 768 1165 1379 1681 2062 678
Size: 18 72 397 214 302 381 2731
Fragments arranged by size:
2731 397 381 302 214 72 18
BsrBI CCG'CTC
Cuts at: 50 2407 2461 3945 4110 50
Size: 2357 54 1484 165 55
Fragments arranged by size:
2357 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2027 2027
Size: 4115
BsrFI r'CCGG_y
Cuts at: 841 1102 2113 2294 2503 841
Size: 261 1011 181 209 2453
Fragments arranged by size:
2453 1011 261 209 181
BssSI C'ACGA_G
Cuts at: 2386 2744 2386
Size: 358 3757
BstAPI GCAn_nnn'nTGC
Cuts at: 783 1492 783
Size: 709 3406
BstBI TT'CG_AA
Cuts at: 2476 2476
Size: 4115
BstEII G'GTnAC_C
Cuts at: 1098 1098
Size: 4115
BstYI r'GATC_y
Cuts at: 1 812 821 905 1004 1140 1607 1630 1965
Size: 811 9 84 99 136 467 23 335
Cuts at: 1965 2211 3223 3309 3321 3667 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 811 467 449 346 335 246 136
99 86 84 23 12 9
BtgI C'CryG_G
Cuts at: 413 413
Size: 4115
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 630 2065 2246 2409 630
Size: 1435 181 163 2336
Fragments arranged by size:
2336 1435 181 163
BtsI GCAGTG_nn'
Cuts at: 150 1358 150
Size: 1208 2907
ClaI AT'CG_AT
Cuts at: 452 452
Size: 4115
DraI TTT'AAA
Cuts at: 1114 1114
Size: 4115
DrdI GACnn_nn'nnGTC
Cuts at: 1821 2679 1821
Size: 858 3257
EaeI y'GGCC_r
Cuts at: 843 1700 1874 2265 2292 3637 843
Size: 857 174 391 27 1345 1321
Fragments arranged by size:
1345 1321 857 391 174 27
EagI C'GGCC_G
Cuts at: 1700 1700
Size: 4115
EarI CTCTTCn'nnn_
Cuts at: 356 2138 2348 356
Size: 1782 210 2123
Fragments arranged by size:
2123 1782 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2323 2633 2779 3925 2323
Size: 310 146 1146 2513
Fragments arranged by size:
2513 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1127 1127
Size: 4115
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1940 2372 3119 3387 1940
Size: 432 747 268 2668
Fragments arranged by size:
2668 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 878 878
Size: 4115
EcoO109I rG'GnC_Cy
Cuts at: 1035 1089 1254 1035
Size: 54 165 3896
Fragments arranged by size:
3896 165 54
EcoRI G'AATT_C
Cuts at: 1119 1119
Size: 4115
EcoRV GAT'ATC
Cuts at: 408 408
Size: 4115
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2134 2134
Size: 4115
FspI TGC'GCA
Cuts at: 1896 1896
Size: 4115
HaeII r_GCGC'y
Cuts at: 304 387 1549 1797 2819 3798 304
Size: 83 1162 248 1022 979 621
Fragments arranged by size:
1162 1022 979 621 248 83
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1957 1987 1989 2019 1957
Size: 30 2 30 4053
Fragments arranged by size:
4053 30 30 2
HincII GTy'rAC
Cuts at: 347 1154 347
Size: 807 3308
KasI G'GCGC_C
Cuts at: 1545 1793 1545
Size: 248 3867
KpnI G_GTAC'C
Cuts at: 1135 1135
Size: 4115
MluI A'CGCG_T
Cuts at: 3548 3548
Size: 4115
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 672 1103 2786 2970 672
Size: 431 1683 184 1817
Fragments arranged by size:
1817 1683 431 184
MscI TGG'CCA
Cuts at: 1876 1876
Size: 4115
MslI CAynn'nnrTG
Cuts at: 676 778 2104 2231 676
Size: 102 1326 127 2560
Fragments arranged by size:
2560 1326 127 102
MspA1I CmG'CkG
Cuts at: 568 834 849 1226 1540 1900 2913 3158 568
Size: 266 15 377 314 360 1013 245 1525
Fragments arranged by size:
1525 1013 377 360 314 266 245 15
NaeI GCC'GGC
Cuts at: 843 2296 843
Size: 1453 2662
NarI GG'CG_CC
Cuts at: 1546 1794 1546
Size: 248 3867
NcoI C'CATG_G
Cuts at: 413 413
Size: 4115
NgoMIV G'CCGG_C
Cuts at: 841 2294 841
Size: 1453 2662
NspI r_CATG'y
Cuts at: 1168 1668 2575 1168
Size: 500 907 2708
Fragments arranged by size:
2708 907 500
PciI A'CATG_T
Cuts at: 2571 2571
Size: 4115
PmeI GTTT'AAAC
Cuts at: 1114 1114
Size: 4115
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3254 3286 3254
Size: 32 4083
PpuMI rG'GwC_Cy
Cuts at: 1035 1035
Size: 4115
PshAI GACnn'nnGTC
Cuts at: 949 949
Size: 4115
PstI C_TGCA'G
Cuts at: 854 1162 1847 854
Size: 308 685 3122
Fragments arranged by size:
3122 685 308
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4115
PvuII CAG'CTG
Cuts at: 834 849 1540 1900 834
Size: 15 691 360 3049
Fragments arranged by size:
3049 691 360 15
RsrII CG'GwC_CG
Cuts at: 2310 2310
Size: 4115
SacI G_AGCT'C
Cuts at: 1129 1129
Size: 4115
SalI G'TCGA_C
Cuts at: 1152 1152
Size: 4115
SapI GCTCTTCn'nnn_
Cuts at: 2138 2348 2138
Size: 210 3905
SbfI CC_TGCA'GG
Cuts at: 1162 1162
Size: 4115
ScaI AGT'ACT
Cuts at: 538 3632 538
Size: 3094 1021
SfcI C'TryA_G
Cuts at: 33 163 394 850 1158 1843 2836 3027 33
Size: 130 231 456 308 685 993 191 1121
Fragments arranged by size:
1121 993 685 456 308 231 191 130
SfoI GGC'GCC
Cuts at: 1547 1795 1547
Size: 248 3867
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4115
SmaI CCC'GGG
Cuts at: 1137 1137
Size: 4115
SmlI C'TyrA_G
Cuts at: 887 1270 2185 2677 2939 887
Size: 383 915 492 262 2063
Fragments arranged by size:
2063 915 492 383 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4115
SphI G_CATG'C
Cuts at: 1168 1668 1168
Size: 500 3615
StyI C'CwwG_G
Cuts at: 317 413 1249 1603 317
Size: 96 836 354 2829
Fragments arranged by size:
2829 836 354 96
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1698 1831 2290 2499 1698
Size: 133 459 209 3314
Fragments arranged by size:
3314 459 209 133
TatI w'GTAC_w
Cuts at: 306 536 3630 306
Size: 230 3094 791
Fragments arranged by size:
3094 791 230
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3632 3664 3632
Size: 32 4083
Tth111I GACn'n_nGTC
Cuts at: 1912 1912
Size: 4115
XbaI T'CTAG_A
Cuts at: 1146 1146
Size: 4115
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 760 985 1610 760
Size: 225 625 3265
Fragments arranged by size:
3265 625 225
XmaI C'CCGG_G
Cuts at: 1135 1135
Size: 4115
XmnI GAAnn'nnTTC
Cuts at: 217 217
Size: 4115
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PmeI PpiI PpuMI PshAI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AloI ApaI AscI AvrII
BaeI BbsI BcgI BfrBI BmgBI BmtI BpmI BsiWI
BsmBI BsrGI BssHII BstXI BstZ17I Bsu36I CspCI DraIII
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PfoI PmlI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI ZraI