Restriction map for
pF1KB3421
(Circular) (Six-base) MAPSORT of: pF1KB3421.seq Check: 34 from: 1 to: 3842
pF1KB3421 3842 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AccI GT'mk_AC
Cuts at: 880 880
Size: 3842
Acc65I G'GTAC_C
Cuts at: 858 858
Size: 3842
AclI AA'CG_TT
Cuts at: 3682 3682
Size: 3842
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 218 1667 2099 2846 3114 218
Size: 1449 432 747 268 946
Fragments arranged by size:
1449 946 747 432 268
AflIII A'CryG_T
Cuts at: 629 1194 2298 3275 629
Size: 565 1104 977 1196
Fragments arranged by size:
1196 1104 977 565
AgeI A'CCGG_T
Cuts at: 2230 2230
Size: 3842
AleI CACnn'nnGTG
Cuts at: 619 619
Size: 3842
AlwNI CAG_nnn'CTG
Cuts at: 1147 2714 1147
Size: 1567 2275
ApaLI G'TGCA_C
Cuts at: 2612 2612
Size: 3842
ApoI r'AATT_y
Cuts at: 846 3195 3451 846
Size: 2349 256 1237
Fragments arranged by size:
2349 1237 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3842
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3842
AvaI C'yCGr_G
Cuts at: 185 862 185
Size: 677 3165
AvrII C'CTAG_G
Cuts at: 435 435
Size: 3842
BamHI G'GATC_C
Cuts at: 867 867
Size: 3842
BanI G'GyrC_C
Cuts at: 858 1272 1520 1555 858
Size: 414 248 35 3145
Fragments arranged by size:
3145 414 248 35
BanII G_rGCy'C
Cuts at: 856 1886 856
Size: 1030 2812
BbeI G_GCGC'C
Cuts at: 1276 1524 1276
Size: 248 3594
BciVI GTATCCnnnnn_n'
Cuts at: 1740 2501 3842 1740
Size: 761 1341 1740
Fragments arranged by size:
1740 1341 761
BclI T'GATC_A
Cuts at: 1362 1362
Size: 3842
BglII A'GATC_T
Cuts at: 1357 3394 1357
Size: 2037 1805
BlpI GC'TnA_GC
Cuts at: 954 954
Size: 3842
Bme1580I G_kGCm'C
Cuts at: 1467 1560 2616 1467
Size: 93 1056 2693
Fragments arranged by size:
2693 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1054 1135 1470 3614 1054
Size: 81 335 2144 1282
Fragments arranged by size:
2144 1282 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2209 2209
Size: 3842
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 233 233
Size: 3842
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1018 1897 2389 2687 1018
Size: 879 492 298 2173
Fragments arranged by size:
2173 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 584 3546 584
Size: 2962 880
BsaAI yAC'GTr
Cuts at: 630 729 1195 630
Size: 99 466 3277
Fragments arranged by size:
3277 466 99
BsaBI GATnn'nnATC
Cuts at: 6 1356 3393 6
Size: 1350 2037 455
Fragments arranged by size:
2037 1350 455
BsaHI Gr'CG_yC
Cuts at: 1273 1521 2223 3719 1273
Size: 248 702 1496 1396
Fragments arranged by size:
1496 1396 702 248
BsaWI w'CCGG_w
Cuts at: 1031 1552 2230 2504 2651 3062 1031
Size: 521 678 274 147 411 1811
Fragments arranged by size:
1811 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 206 236 581 611 206
Size: 30 345 30 3437
Fragments arranged by size:
3437 345 30 30
BseYI C'CCAG_C
Cuts at: 651 818 1267 2025 2602 3098 651
Size: 167 449 758 577 496 1395
Fragments arranged by size:
1395 758 577 496 449 167
BsiEI CG_ry'CG
Cuts at: 104 1384 1430 2638 104
Size: 1280 46 1208 1308
Fragments arranged by size:
1308 1280 1208 46
BsiHKAI G_wGCw'C
Cuts at: 856 1634 1824 2616 856
Size: 778 190 792 2082
Fragments arranged by size:
2082 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 856 1467 1560 1634 1824 1886 2616 856
Size: 611 93 74 190 62 730 2082
Fragments arranged by size:
2082 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3062 3062
Size: 3842
BspHI T'CATG_A
Cuts at: 3018 3018
Size: 3842
BspMI ACCTGCnnnn'nnnn_
Cuts at: 892 1106 1408 1789 892
Size: 214 302 381 2945
Fragments arranged by size:
2945 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2134 2188 3672 3837 50
Size: 2084 54 1484 165 55
Fragments arranged by size:
2084 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 401 1754 401
Size: 1353 2489
BsrFI r'CCGG_y
Cuts at: 296 1840 2021 2230 296
Size: 1544 181 209 1908
Fragments arranged by size:
1908 1544 209 181
BssSI C'ACGA_G
Cuts at: 2113 2471 2113
Size: 358 3484
BstAPI GCAn_nnn'nTGC
Cuts at: 1219 1219
Size: 3842
BstBI TT'CG_AA
Cuts at: 2203 2203
Size: 3842
BstYI r'GATC_y
Cuts at: 1 867 1334 1357 1692 1938 2950 3036 3048
Size: 866 467 23 335 246 1012 86 12
Cuts at: 3048 3394 1
Size: 346 449
Fragments arranged by size:
1012 866 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3842
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1792 1973 2136 1792
Size: 181 163 3498
Fragments arranged by size:
3498 181 163
BtsI GCAGTG_nn'
Cuts at: 119 360 614 1085 119
Size: 241 254 471 2876
Fragments arranged by size:
2876 471 254 241
DraI TTT'AAA
Cuts at: 841 841
Size: 3842
DrdI GACnn_nn'nnGTC
Cuts at: 1548 2406 1548
Size: 858 2984
EaeI y'GGCC_r
Cuts at: 380 1427 1601 1992 2019 3364 380
Size: 1047 174 391 27 1345 858
Fragments arranged by size:
1345 1047 858 391 174 27
EagI C'GGCC_G
Cuts at: 1427 1427
Size: 3842
EarI CTCTTCn'nnn_
Cuts at: 124 325 1865 2075 124
Size: 201 1540 210 1891
Fragments arranged by size:
1891 1540 210 201
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 315 2050 2360 2506 3652 315
Size: 1735 310 146 1146 505
Fragments arranged by size:
1735 1146 505 310 146
EcoICRI GAG'CTC
Cuts at: 854 854
Size: 3842
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 218 233 1667 2099 2846 3114 218
Size: 15 1434 432 747 268 946
Fragments arranged by size:
1434 946 747 432 268 15
EcoO109I rG'GnC_Cy
Cuts at: 458 684 981 458
Size: 226 297 3319
Fragments arranged by size:
3319 297 226
EcoRI G'AATT_C
Cuts at: 846 846
Size: 3842
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1861 1861
Size: 3842
FspI TGC'GCA
Cuts at: 1623 1623
Size: 3842
HaeII r_GCGC'y
Cuts at: 1276 1524 2546 3525 1276
Size: 248 1022 979 1593
Fragments arranged by size:
1593 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 193 225 1684 1714 1716 1746 193
Size: 32 1459 30 2 30 2289
Fragments arranged by size:
2289 1459 32 30 30 2
HincII GTy'rAC
Cuts at: 600 612 881 600
Size: 12 269 3561
Fragments arranged by size:
3561 269 12
HpaI GTT'AAC
Cuts at: 600 600
Size: 3842
KasI G'GCGC_C
Cuts at: 1272 1520 1272
Size: 248 3594
KpnI G_GTAC'C
Cuts at: 862 862
Size: 3842
MluI A'CGCG_T
Cuts at: 3275 3275
Size: 3842
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2513 2697 2513
Size: 184 3658
MscI TGG'CCA
Cuts at: 382 1603 382
Size: 1221 2621
MslI CAynn'nnrTG
Cuts at: 112 619 661 1831 1958 112
Size: 507 42 1170 127 1996
Fragments arranged by size:
1996 1170 507 127 42
MspA1I CmG'CkG
Cuts at: 587 953 1267 1627 2640 2885 587
Size: 366 314 360 1013 245 1544
Fragments arranged by size:
1544 1013 366 360 314 245
NaeI GCC'GGC
Cuts at: 298 2023 298
Size: 1725 2117
NarI GG'CG_CC
Cuts at: 1273 1521 1273
Size: 248 3594
NcoI C'CATG_G
Cuts at: 107 107
Size: 3842
NgoMIV G'CCGG_C
Cuts at: 296 2021 296
Size: 1725 2117
NspI r_CATG'y
Cuts at: 895 1395 2302 895
Size: 500 907 2435
Fragments arranged by size:
2435 907 500
PasI CC'CwG_GG
Cuts at: 680 680
Size: 3842
PciI A'CATG_T
Cuts at: 2298 2298
Size: 3842
PmeI GTTT'AAAC
Cuts at: 841 841
Size: 3842
PmlI CAC'GTG
Cuts at: 630 729 630
Size: 99 3743
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2981 3013 2981
Size: 32 3810
PpuMI rG'GwC_Cy
Cuts at: 684 684
Size: 3842
PstI C_TGCA'G
Cuts at: 889 1574 889
Size: 685 3157
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3842
PvuII CAG'CTG
Cuts at: 1267 1627 1267
Size: 360 3482
RsrII CG'GwC_CG
Cuts at: 2037 2037
Size: 3842
SacI G_AGCT'C
Cuts at: 856 856
Size: 3842
SalI G'TCGA_C
Cuts at: 879 879
Size: 3842
SanDI GG'GwC_CC
Cuts at: 684 684
Size: 3842
SapI GCTCTTCn'nnn_
Cuts at: 1865 2075 1865
Size: 210 3632
SbfI CC_TGCA'GG
Cuts at: 889 889
Size: 3842
ScaI AGT'ACT
Cuts at: 3359 3359
Size: 3842
SfcI C'TryA_G
Cuts at: 33 320 885 1570 2563 2754 33
Size: 287 565 685 993 191 1121
Fragments arranged by size:
1121 993 685 565 287 191
SfoI GGC'GCC
Cuts at: 1274 1522 1274
Size: 248 3594
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3842
SmaI CCC'GGG
Cuts at: 864 864
Size: 3842
SmlI C'TyrA_G
Cuts at: 997 1912 2404 2666 997
Size: 915 492 262 2173
Fragments arranged by size:
2173 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3842
SphI G_CATG'C
Cuts at: 895 1395 895
Size: 500 3342
StyI C'CwwG_G
Cuts at: 107 435 976 1330 107
Size: 328 541 354 2619
Fragments arranged by size:
2619 541 354 328
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1425 1558 2017 2226 1425
Size: 133 459 209 3041
Fragments arranged by size:
3041 459 209 133
TatI w'GTAC_w
Cuts at: 3357 3357
Size: 3842
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 206 238 3359 3391 206
Size: 32 3121 32 657
Fragments arranged by size:
3121 657 32 32
Tth111I GACn'n_nGTC
Cuts at: 1639 1639
Size: 3842
XbaI T'CTAG_A
Cuts at: 873 873
Size: 3842
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 561 1337 561
Size: 776 3066
XmaI C'CCGG_G
Cuts at: 862 862
Size: 3842
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HpaI KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PmeI PmlI PpiI PpuMI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI BaeI BbsI BbvCI BcgI BfrBI BglI BmgBI
BmtI Bpu10I BseRI BsgI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII MfeI NdeI NheI
NotI NruI NsiI PacI PflMI PfoI PshAI PsiI
PspOMI PspXI PsrI SacII SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI