Restriction map for
pF1KB3416
(Circular) (Six-base) MAPSORT of: pF1KB3416.seq Check: 9463 from: 1 to: 3524
pF1KB3416 3524 bp
With 182 enzymes: SgfI *
April 24, 2009 13:01 ..
AccI GT'mk_AC
Cuts at: 562 562
Size: 3524
Acc65I G'GTAC_C
Cuts at: 540 540
Size: 3524
AclI AA'CG_TT
Cuts at: 3364 3364
Size: 3524
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1349 1781 2528 2796 1349
Size: 432 747 268 2077
Fragments arranged by size:
2077 747 432 268
AflIII A'CryG_T
Cuts at: 876 1980 2957 876
Size: 1104 977 1443
Fragments arranged by size:
1443 1104 977
AgeI A'CCGG_T
Cuts at: 1912 1912
Size: 3524
AlwNI CAG_nnn'CTG
Cuts at: 829 2396 829
Size: 1567 1957
ApaI G_GGCC'C
Cuts at: 351 351
Size: 3524
ApaLI G'TGCA_C
Cuts at: 2294 2294
Size: 3524
ApoI r'AATT_y
Cuts at: 528 2877 3133 528
Size: 2349 256 919
Fragments arranged by size:
2349 919 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3524
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3524
AvaI C'yCGr_G
Cuts at: 544 544
Size: 3524
BamHI G'GATC_C
Cuts at: 323 549 323
Size: 226 3298
BanI G'GyrC_C
Cuts at: 540 954 1202 1237 540
Size: 414 248 35 2827
Fragments arranged by size:
2827 414 248 35
BanII G_rGCy'C
Cuts at: 265 351 538 1568 265
Size: 86 187 1030 2221
Fragments arranged by size:
2221 1030 187 86
BbeI G_GCGC'C
Cuts at: 958 1206 958
Size: 248 3276
BciVI GTATCCnnnnn_n'
Cuts at: 1422 2183 3524 1422
Size: 761 1341 1422
Fragments arranged by size:
1422 1341 761
BclI T'GATC_A
Cuts at: 416 1044 416
Size: 628 2896
BglII A'GATC_T
Cuts at: 1039 3076 1039
Size: 2037 1487
BlpI GC'TnA_GC
Cuts at: 636 636
Size: 3524
Bme1580I G_kGCm'C
Cuts at: 351 453 1149 1242 2298 351
Size: 102 696 93 1056 1577
Fragments arranged by size:
1577 1056 696 102 93
BmrI ACTGGGnnnn_n'
Cuts at: 736 817 1152 3296 736
Size: 81 335 2144 964
Fragments arranged by size:
2144 964 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 316 1891 316
Size: 1575 1949
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 700 1579 2071 2369 700
Size: 879 492 298 1855
Fragments arranged by size:
1855 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3228 3228
Size: 3524
BsaAI yAC'GTr
Cuts at: 454 877 454
Size: 423 3101
BsaBI GATnn'nnATC
Cuts at: 6 1038 3075 6
Size: 1032 2037 455
Fragments arranged by size:
2037 1032 455
BsaHI Gr'CG_yC
Cuts at: 955 1203 1905 3401 955
Size: 248 702 1496 1078
Fragments arranged by size:
1496 1078 702 248
BsaWI w'CCGG_w
Cuts at: 713 1234 1912 2186 2333 2744 713
Size: 521 678 274 147 411 1493
Fragments arranged by size:
1493 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 118 118
Size: 3524
BseYI C'CCAG_C
Cuts at: 258 949 1707 2284 2780 258
Size: 691 758 577 496 1002
Fragments arranged by size:
1002 758 691 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 168 168
Size: 3524
BsiEI CG_ry'CG
Cuts at: 104 196 1066 1112 2320 104
Size: 92 870 46 1208 1308
Fragments arranged by size:
1308 1208 870 92 46
BsiHKAI G_wGCw'C
Cuts at: 538 1316 1506 2298 538
Size: 778 190 792 1764
Fragments arranged by size:
1764 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 265 351 453 538 1149 1242 1316 1506 1568
Size: 86 102 85 611 93 74 190 62
Cuts at: 1568 2298 265
Size: 730 1491
Fragments arranged by size:
1491 730 611 190 102 93 86 85
74 62
BspEI T'CCGG_A
Cuts at: 2744 2744
Size: 3524
BspHI T'CATG_A
Cuts at: 2700 2700
Size: 3524
BspMI ACCTGCnnnn'nnnn_
Cuts at: 574 788 1090 1471 574
Size: 214 302 381 2627
Fragments arranged by size:
2627 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1816 1870 3354 3519 50
Size: 1766 54 1484 165 55
Fragments arranged by size:
1766 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 274 1436 274
Size: 1162 2362
BsrFI r'CCGG_y
Cuts at: 1522 1703 1912 1522
Size: 181 209 3134
Fragments arranged by size:
3134 209 181
BssSI C'ACGA_G
Cuts at: 1795 2153 1795
Size: 358 3166
BstAPI GCAn_nnn'nTGC
Cuts at: 901 901
Size: 3524
BstBI TT'CG_AA
Cuts at: 1885 1885
Size: 3524
BstYI r'GATC_y
Cuts at: 1 323 549 1016 1039 1374 1620 2632 2718
Size: 322 226 467 23 335 246 1012 86
Cuts at: 2718 2730 3076 1
Size: 12 346 449
Fragments arranged by size:
1012 467 449 346 335 322 246 226
86 23 12
BtgI C'CryG_G
Cuts at: 107 144 107
Size: 37 3487
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1474 1655 1818 1474
Size: 181 163 3180
Fragments arranged by size:
3180 181 163
BtsI GCAGTG_nn'
Cuts at: 767 767
Size: 3524
DraI TTT'AAA
Cuts at: 523 523
Size: 3524
DrdI GACnn_nn'nnGTC
Cuts at: 1230 2088 1230
Size: 858 2666
EaeI y'GGCC_r
Cuts at: 193 240 1109 1283 1674 1701 3046 193
Size: 47 869 174 391 27 1345 671
Fragments arranged by size:
1345 869 671 391 174 47 27
EagI C'GGCC_G
Cuts at: 193 1109 193
Size: 916 2608
EarI CTCTTCn'nnn_
Cuts at: 1547 1757 1547
Size: 210 3314
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 154 1732 2042 2188 3334 154
Size: 1578 310 146 1146 344
Fragments arranged by size:
1578 1146 344 310 146
EcoICRI GAG'CTC
Cuts at: 536 536
Size: 3524
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1349 1781 2528 2796 1349
Size: 432 747 268 2077
Fragments arranged by size:
2077 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 133 133
Size: 3524
EcoO109I rG'GnC_Cy
Cuts at: 347 663 347
Size: 316 3208
EcoRI G'AATT_C
Cuts at: 528 528
Size: 3524
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1543 1543
Size: 3524
FspI TGC'GCA
Cuts at: 1305 1305
Size: 3524
HaeII r_GCGC'y
Cuts at: 958 1206 2228 3207 958
Size: 248 1022 979 1275
Fragments arranged by size:
1275 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1366 1396 1398 1428 1366
Size: 30 2 30 3462
Fragments arranged by size:
3462 30 30 2
HincII GTy'rAC
Cuts at: 563 563
Size: 3524
HindIII A'AGCT_T
Cuts at: 373 373
Size: 3524
KasI G'GCGC_C
Cuts at: 954 1202 954
Size: 248 3276
KpnI G_GTAC'C
Cuts at: 544 544
Size: 3524
MluI A'CGCG_T
Cuts at: 2957 2957
Size: 3524
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2195 2379 2195
Size: 184 3340
MscI TGG'CCA
Cuts at: 1285 1285
Size: 3524
MslI CAynn'nnrTG
Cuts at: 1513 1640 1513
Size: 127 3397
MspA1I CmG'CkG
Cuts at: 258 635 949 1309 2322 2567 258
Size: 377 314 360 1013 245 1215
Fragments arranged by size:
1215 1013 377 360 314 245
NaeI GCC'GGC
Cuts at: 1705 1705
Size: 3524
NarI GG'CG_CC
Cuts at: 955 1203 955
Size: 248 3276
NcoI C'CATG_G
Cuts at: 107 107
Size: 3524
NgoMIV G'CCGG_C
Cuts at: 1703 1703
Size: 3524
NspI r_CATG'y
Cuts at: 577 1077 1984 577
Size: 500 907 2117
Fragments arranged by size:
2117 907 500
PasI CC'CwG_GG
Cuts at: 174 233 174
Size: 59 3465
PciI A'CATG_T
Cuts at: 1980 1980
Size: 3524
PmeI GTTT'AAAC
Cuts at: 523 523
Size: 3524
PmlI CAC'GTG
Cuts at: 454 454
Size: 3524
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2663 2695 2663
Size: 32 3492
PspOMI G'GGCC_C
Cuts at: 347 347
Size: 3524
PstI C_TGCA'G
Cuts at: 571 1256 571
Size: 685 2839
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3524
PvuII CAG'CTG
Cuts at: 258 949 1309 258
Size: 691 360 2473
Fragments arranged by size:
2473 691 360
RsrII CG'GwC_CG
Cuts at: 1719 1719
Size: 3524
SacI G_AGCT'C
Cuts at: 538 538
Size: 3524
SalI G'TCGA_C
Cuts at: 561 561
Size: 3524
SapI GCTCTTCn'nnn_
Cuts at: 1547 1757 1547
Size: 210 3314
SbfI CC_TGCA'GG
Cuts at: 571 571
Size: 3524
ScaI AGT'ACT
Cuts at: 3041 3041
Size: 3524
SfcI C'TryA_G
Cuts at: 33 494 509 567 1252 2245 2436 33
Size: 461 15 58 685 993 191 1121
Fragments arranged by size:
1121 993 685 461 191 58 15
SfoI GGC'GCC
Cuts at: 956 1204 956
Size: 248 3276
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3524
SmaI CCC'GGG
Cuts at: 546 546
Size: 3524
SmlI C'TyrA_G
Cuts at: 679 1594 2086 2348 679
Size: 915 492 262 1855
Fragments arranged by size:
1855 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3524
SphI G_CATG'C
Cuts at: 577 1077 577
Size: 500 3024
StuI AGG'CCT
Cuts at: 139 139
Size: 3524
StyI C'CwwG_G
Cuts at: 107 658 1012 107
Size: 551 354 2619
Fragments arranged by size:
2619 551 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 166 1107 1240 1699 1908 166
Size: 941 133 459 209 1782
Fragments arranged by size:
1782 941 459 209 133
TatI w'GTAC_w
Cuts at: 3039 3039
Size: 3524
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 443 475 3041 3073 443
Size: 32 2566 32 894
Fragments arranged by size:
2566 894 32 32
Tth111I GACn'n_nGTC
Cuts at: 1321 1321
Size: 3524
XbaI T'CTAG_A
Cuts at: 555 555
Size: 3524
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1019 1019
Size: 3524
XmaI C'CCGG_G
Cuts at: 544 544
Size: 3524
XmnI GAAnn'nnTTC
Cuts at: 374 374
Size: 3524
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PmeI PmlI PpiI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI BpmI Bpu10I BsaXI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI
DraIII EcoRV FseI FspAI HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PflMI PfoI PpuMI PshAI
PsiI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI SwaI XhoI ZraI