Restriction map for
pF1KB3414
(Circular) (Six-base) MAPSORT of: pF1KB3414.seq Check: 7106 from: 1 to: 5741
pF1KB3414 5741 bp
With 182 enzymes: SgfI *
September 4, 2008 14:04 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 756 1186 756
Size: 430 5311
AccI GT'mk_AC
Cuts at: 282 553 1448 2779 282
Size: 271 895 1331 3244
Fragments arranged by size:
3244 1331 895 271
Acc65I G'GTAC_C
Cuts at: 1250 2757 1250
Size: 1507 4234
AclI AA'CG_TT
Cuts at: 5581 5581
Size: 5741
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 983 1448 1562 1777 1831 1855 2308 2672 2743
Size: 465 114 215 54 24 453 364 71
Cuts at: 2743 3566 3998 4351 5013 983
Size: 823 432 353 662 1711
Fragments arranged by size:
1711 823 662 465 453 432 364 353
215 114 71 54 24
AflIII A'CryG_T
Cuts at: 1203 3093 4893 5174 1203
Size: 1890 1800 281 1770
Fragments arranged by size:
1890 1800 1770 281
AhdI GACnn_n'nnGTC
Cuts at: 376 1848 376
Size: 1472 4269
AleI CACnn'nnGTG
Cuts at: 2011 2011
Size: 5741
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1173 1173
Size: 5741
AlwNI CAG_nnn'CTG
Cuts at: 828 1071 1473 2247 3046 4484 828
Size: 243 402 774 799 1438 2085
Fragments arranged by size:
2085 1438 799 774 402 243
ApaLI G'TGCA_C
Cuts at: 4579 4579
Size: 5741
ApoI r'AATT_y
Cuts at: 949 1409 2032 2202 2491 2745 5094 5350 949
Size: 460 623 170 289 254 2349 256 1340
Fragments arranged by size:
2349 1340 623 460 289 256 254 170
AseI AT'TA_AT
Cuts at: 20 1354 20
Size: 1334 4407
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5741
AvaI C'yCGr_G
Cuts at: 127 2761 127
Size: 2634 3107
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 788 821 1051 1084 788
Size: 33 230 33 5445
Fragments arranged by size:
5445 230 33 33
BamHI G'GATC_C
Cuts at: 2766 2766
Size: 5741
BanI G'GyrC_C
Cuts at: 1250 2757 3171 3419 3454 1250
Size: 1507 414 248 35 3537
Fragments arranged by size:
3537 1507 414 248 35
BanII G_rGCy'C
Cuts at: 1304 2755 3785 1304
Size: 1451 1030 3260
Fragments arranged by size:
3260 1451 1030
BbeI G_GCGC'C
Cuts at: 3175 3423 3175
Size: 248 5493
BbsI GAAGACnn'nnnn_
Cuts at: 139 2674 139
Size: 2535 3206
BbvCI CC'TCA_GC
Cuts at: 500 500
Size: 5741
BciVI GTATCCnnnnn_n'
Cuts at: 3639 4695 5741 3639
Size: 1056 1046 3639
Fragments arranged by size:
3639 1056 1046
BclI T'GATC_A
Cuts at: 116 1575 3261 116
Size: 1459 1686 2596
Fragments arranged by size:
2596 1686 1459
BglI GCCn_nnn'nGGC
Cuts at: 1996 1996
Size: 5741
BglII A'GATC_T
Cuts at: 1913 3256 5293 1913
Size: 1343 2037 2361
Fragments arranged by size:
2361 2037 1343
BlpI GC'TnA_GC
Cuts at: 2853 2853
Size: 5741
Bme1580I G_kGCm'C
Cuts at: 2148 3366 3459 4583 2148
Size: 1218 93 1124 3306
Fragments arranged by size:
3306 1218 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 466 1298 1361 2953 3034 3369 5513 466
Size: 832 63 1592 81 335 2144 694
Fragments arranged by size:
2144 1592 832 694 335 81 63
BmtI G_CTAG'C
Cuts at: 685 685
Size: 5741
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4108 4108
Size: 5741
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 175 202 1515 2543 175
Size: 27 1313 1028 3373
Fragments arranged by size:
3373 1313 1028 27
Bpu10I CC'TnA_GC
Cuts at: 500 1028 2389 500
Size: 528 1361 3852
Fragments arranged by size:
3852 1361 528
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2023 2917 3796 4510 4808 2023
Size: 894 879 714 298 2956
Fragments arranged by size:
2956 894 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 1489 5445 1489
Size: 3956 1785
BsaAI yAC'GTr
Cuts at: 3094 3094
Size: 5741
BsaBI GATnn'nnATC
Cuts at: 6 1736 2531 3255 5292 6
Size: 1730 795 724 2037 455
Fragments arranged by size:
2037 1730 795 724 455
BsaHI Gr'CG_yC
Cuts at: 3172 3420 4122 5618 3172
Size: 248 702 1496 3295
Fragments arranged by size:
3295 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2930 3451 4129 4540 4687 4961 2930
Size: 521 678 411 147 274 3710
Fragments arranged by size:
3710 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 126 156 1193 1223 126
Size: 30 1037 30 4644
Fragments arranged by size:
4644 1037 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1695 2297 1695
Size: 602 5139
BseYI C'CCAG_C
Cuts at: 1520 1963 3166 3924 4589 4997 1520
Size: 443 1203 758 665 408 2264
Fragments arranged by size:
2264 1203 758 665 443 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 482 482
Size: 5741
BsiEI CG_ry'CG
Cuts at: 104 150 194 3283 3329 4559 104
Size: 46 44 3089 46 1230 1286
Fragments arranged by size:
3089 1286 1230 46 46 44
BsiHKAI G_wGCw'C
Cuts at: 542 1034 1062 1819 2755 3533 3723 4583 542
Size: 492 28 757 936 778 190 860 1700
Fragments arranged by size:
1700 936 860 778 757 492 190 28
BsmI GAATG_Cn'
Cuts at: 699 699
Size: 5741
Bsp1286I G_dGCh'C
Cuts at: 542 1034 1062 1304 1819 2148 2755 3366 3459
Size: 492 28 242 515 329 607 611 93
Cuts at: 3459 3533 3723 3785 4583 542
Size: 74 190 62 798 1700
Fragments arranged by size:
1700 798 611 607 515 492 329 242
190 93 74 62 28
BspHI T'CATG_A
Cuts at: 4173 4173
Size: 5741
BspMI ACCTGCnnnn'nnnn_
Cuts at: 212 756 1186 2791 3005 3307 3688 212
Size: 544 430 1605 214 302 381 2265
Fragments arranged by size:
2265 1605 544 430 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4033 4087 5571 5736 50
Size: 3983 54 1484 165 55
Fragments arranged by size:
3983 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 914 2516 3653 914
Size: 1602 1137 3002
Fragments arranged by size:
3002 1602 1137
BsrFI r'CCGG_y
Cuts at: 419 3739 3920 419
Size: 3320 181 2240
Fragments arranged by size:
3320 2240 181
BsrGI T'GTAC_A
Cuts at: 2584 2584
Size: 5741
BssSI C'ACGA_G
Cuts at: 4012 4720 4012
Size: 708 5033
BstAPI GCAn_nnn'nTGC
Cuts at: 1567 3118 1567
Size: 1551 4190
BstBI TT'CG_AA
Cuts at: 4102 4102
Size: 5741
BstXI CCAn_nnnn'nTGG
Cuts at: 237 2521 237
Size: 2284 3457
BstYI r'GATC_y
Cuts at: 1 312 467 1913 2766 3233 3256 3591 3837
Size: 311 155 1446 853 467 23 335 246
Cuts at: 3837 4143 4155 4241 5293 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1446 1052 853 467 449 335 311 306
246 155 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1678 1678
Size: 5741
BtgI C'CryG_G
Cuts at: 107 194 107
Size: 87 5654
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3691 3872 4035 3691
Size: 181 163 5397
Fragments arranged by size:
5397 181 163
BtsI GCAGTG_nn'
Cuts at: 1271 2984 1271
Size: 1713 4028
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 198 233 198
Size: 35 5706
DraI TTT'AAA
Cuts at: 2058 2292 2740 2058
Size: 234 448 5059
Fragments arranged by size:
5059 448 234
DraIII CAC_nnn'GTG
Cuts at: 442 788 442
Size: 346 5395
DrdI GACnn_nn'nnGTC
Cuts at: 3447 4791 3447
Size: 1344 4397
EaeI y'GGCC_r
Cuts at: 191 484 509 986 1172 3326 3500 3891 3918
Size: 293 25 477 186 2154 174 391 27
Cuts at: 3918 5263 191
Size: 1345 669
Fragments arranged by size:
2154 1345 669 477 391 293 186 174
27 25
EagI C'GGCC_G
Cuts at: 191 3326 191
Size: 3135 2606
EarI CTCTTCn'nnn_
Cuts at: 653 1874 2327 3764 3974 653
Size: 1221 453 1437 210 2420
Fragments arranged by size:
2420 1437 1221 453 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2673 3949 4691 4837 5551 2673
Size: 1276 742 146 714 2863
Fragments arranged by size:
2863 1276 742 714 146
EcoICRI GAG'CTC
Cuts at: 2753 2753
Size: 5741
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 175 202 983 1448 1515 1562 1777 1831 1855
Size: 27 781 465 67 47 215 54 24
Cuts at: 1855 2308 2543 2672 2743 3566 3998 4351 5013
Size: 453 235 129 71 823 432 353 662
Cuts at: 5013 175
Size: 903
Fragments arranged by size:
903 823 781 662 465 453 432 353
235 215 129 71 67 54 47 27
24
EcoO109I rG'GnC_Cy
Cuts at: 877 2880 877
Size: 2003 3738
EcoRI G'AATT_C
Cuts at: 949 2032 2745 949
Size: 1083 713 3945
Fragments arranged by size:
3945 1083 713
EcoRV GAT'ATC
Cuts at: 321 321
Size: 5741
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3760 3760
Size: 5741
FspI TGC'GCA
Cuts at: 3522 3522
Size: 5741
HaeII r_GCGC'y
Cuts at: 3175 3423 4653 5424 3175
Size: 248 1230 771 3492
Fragments arranged by size:
3492 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 126 158 362 394 727 759 1467 1499 1834
Size: 32 204 32 333 32 708 32 335
Cuts at: 1834 1866 3583 3613 3615 3645 126
Size: 32 1717 30 2 30 2222
Fragments arranged by size:
2222 1717 708 335 333 204 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 554 2648 2780 554
Size: 2094 132 3515
Fragments arranged by size:
3515 2094 132
HindIII A'AGCT_T
Cuts at: 995 995
Size: 5741
HpaI GTT'AAC
Cuts at: 2648 2648
Size: 5741
KasI G'GCGC_C
Cuts at: 3171 3419 3171
Size: 248 5493
KpnI G_GTAC'C
Cuts at: 1254 2761 1254
Size: 1507 4234
MluI A'CGCG_T
Cuts at: 5174 5174
Size: 5741
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2180 4500 4684 2180
Size: 2320 184 3237
Fragments arranged by size:
3237 2320 184
MscI TGG'CCA
Cuts at: 486 511 988 1174 3502 486
Size: 25 477 186 2328 2725
Fragments arranged by size:
2725 2328 477 186 25
MslI CAynn'nnrTG
Cuts at: 1901 2011 2462 2540 3730 3857 1901
Size: 110 451 78 1190 127 3785
Fragments arranged by size:
3785 1190 451 127 110 78
MspA1I CmG'CkG
Cuts at: 277 504 1390 1963 2116 2190 2852 3166 3526
Size: 227 886 573 153 74 662 314 360
Cuts at: 3526 4310 4555 277
Size: 784 245 1463
Fragments arranged by size:
1463 886 784 662 573 360 314 245
227 153 74
NaeI GCC'GGC
Cuts at: 3922 3922
Size: 5741
NarI GG'CG_CC
Cuts at: 3172 3420 3172
Size: 248 5493
NcoI C'CATG_G
Cuts at: 107 107
Size: 5741
NdeI CA'TA_TG
Cuts at: 1692 1692
Size: 5741
NgoMIV G'CCGG_C
Cuts at: 3920 3920
Size: 5741
NheI G'CTAG_C
Cuts at: 681 681
Size: 5741
NspI r_CATG'y
Cuts at: 1207 2794 3294 4897 1207
Size: 1587 500 1603 2051
Fragments arranged by size:
2051 1603 1587 500
PciI A'CATG_T
Cuts at: 1203 4893 1203
Size: 3690 2051
PfoI T'CCnGG_A
Cuts at: 179 246 179
Size: 67 5674
PmeI GTTT'AAAC
Cuts at: 2740 2740
Size: 5741
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4187 4219 4187
Size: 32 5709
PpuMI rG'GwC_Cy
Cuts at: 877 877
Size: 5741
PshAI GACnn'nnGTC
Cuts at: 1762 1762
Size: 5741
PstI C_TGCA'G
Cuts at: 1563 2788 3473 1563
Size: 1225 685 3831
Fragments arranged by size:
3831 1225 685
PvuI CG_AT'CG
Cuts at: 104 150 104
Size: 46 5695
PvuII CAG'CTG
Cuts at: 277 504 1390 1963 2116 2190 3166 3526 277
Size: 227 886 573 153 74 976 360 2492
Fragments arranged by size:
2492 976 886 573 360 227 153 74
RsrII CG'GwC_CG
Cuts at: 3936 3936
Size: 5741
SacI G_AGCT'C
Cuts at: 2755 2755
Size: 5741
SalI G'TCGA_C
Cuts at: 552 2778 552
Size: 2226 3515
SapI GCTCTTCn'nnn_
Cuts at: 653 3764 3974 653
Size: 3111 210 2420
Fragments arranged by size:
3111 2420 210
SbfI CC_TGCA'GG
Cuts at: 2788 2788
Size: 5741
ScaI AGT'ACT
Cuts at: 5258 5258
Size: 5741
SexAI A'CCwGG_T
Cuts at: 2099 2099
Size: 5741
SfcI C'TryA_G
Cuts at: 33 1063 1400 1465 1559 1646 2784 3469 4437
Size: 1030 337 65 94 87 1138 685 968
Cuts at: 4437 4628 33
Size: 191 1146
Fragments arranged by size:
1146 1138 1030 968 685 337 191 94
87 65
SfoI GGC'GCC
Cuts at: 3173 3421 3173
Size: 248 5493
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5741
SmaI CCC'GGG
Cuts at: 2763 2763
Size: 5741
SmlI C'TyrA_G
Cuts at: 127 2038 2896 3811 4525 4787 127
Size: 1911 858 915 714 262 1081
Fragments arranged by size:
1911 1081 915 858 714 262
SpeI A'CTAG_T
Cuts at: 67 1320 2010 67
Size: 1253 690 3798
Fragments arranged by size:
3798 1253 690
SphI G_CATG'C
Cuts at: 2794 3294 2794
Size: 500 5241
StuI AGG'CCT
Cuts at: 2053 2332 2053
Size: 279 5462
StyI C'CwwG_G
Cuts at: 107 1727 2875 3229 107
Size: 1620 1148 354 2619
Fragments arranged by size:
2619 1620 1148 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2014 3324 3457 3916 4125 2014
Size: 1310 133 459 209 3630
Fragments arranged by size:
3630 1310 459 209 133
TatI w'GTAC_w
Cuts at: 857 1977 2042 2584 2604 5256 857
Size: 1120 65 542 20 2652 1342
Fragments arranged by size:
2652 1342 1120 542 65 20
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 774 806 1193 1225 5258 5290 774
Size: 32 387 32 4033 32 1225
Fragments arranged by size:
4033 1225 387 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 138 1195 3538 138
Size: 1057 2343 2341
Fragments arranged by size:
2343 2341 1057
XbaI T'CTAG_A
Cuts at: 2772 2772
Size: 5741
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 216 479 1960 3236 216
Size: 263 1481 1276 2721
Fragments arranged by size:
2721 1481 1276 263
XhoI C'TCGA_G
Cuts at: 127 127
Size: 5741
XmaI C'CCGG_G
Cuts at: 2761 2761
Size: 5741
XmnI GAAnn'nnTTC
Cuts at: 494 2495 494
Size: 2001 3740
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AhdI AleI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NspI
PciI PfoI PmeI PpiI PpuMI PshAI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AgeI AloI ApaI AscI AvrII
BcgI BfrBI BmgBI BsiWI BsmBI BspEI BssHII BstEII
BstZ17I ClaI EcoNI FseI FspAI MfeI NotI NruI
NsiI PacI PasI PflMI PmlI PsiI PspOMI PspXI
PsrI SacII SanDI SfiI SgrAI SnaBI SrfI SspI
SwaI ZraI