Restriction map for
pF1KB3413
(Circular) (Six-base) MAPSORT of: pF1KB3413.seq Check: 4473 from: 1 to: 4130
pF1KB3413 4130 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AccI GT'mk_AC
Cuts at: 1168 1168
Size: 4130
Acc65I G'GTAC_C
Cuts at: 1146 1146
Size: 4130
AclI AA'CG_TT
Cuts at: 3970 3970
Size: 4130
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 299 1136 1955 2387 3134 3402 299
Size: 837 819 432 747 268 1027
Fragments arranged by size:
1027 837 819 747 432 268
AflIII A'CryG_T
Cuts at: 1482 2586 3563 1482
Size: 1104 977 2049
Fragments arranged by size:
2049 1104 977
AgeI A'CCGG_T
Cuts at: 2518 2518
Size: 4130
AlwNI CAG_nnn'CTG
Cuts at: 732 1435 3002 732
Size: 703 1567 1860
Fragments arranged by size:
1860 1567 703
ApaI G_GGCC'C
Cuts at: 450 450
Size: 4130
ApaLI G'TGCA_C
Cuts at: 2900 2900
Size: 4130
ApoI r'AATT_y
Cuts at: 1134 3483 3739 1134
Size: 2349 256 1525
Fragments arranged by size:
2349 1525 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4130
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4130
AvaI C'yCGr_G
Cuts at: 409 717 1150 409
Size: 308 433 3389
Fragments arranged by size:
3389 433 308
BamHI G'GATC_C
Cuts at: 951 963 1155 951
Size: 12 192 3926
Fragments arranged by size:
3926 192 12
BanI G'GyrC_C
Cuts at: 581 1146 1560 1808 1843 581
Size: 565 414 248 35 2868
Fragments arranged by size:
2868 565 414 248 35
BanII G_rGCy'C
Cuts at: 450 599 906 1144 2174 450
Size: 149 307 238 1030 2406
Fragments arranged by size:
2406 1030 307 238 149
BbeI G_GCGC'C
Cuts at: 1564 1812 1564
Size: 248 3882
BciVI GTATCCnnnnn_n'
Cuts at: 2028 2789 4130 2028
Size: 761 1341 2028
Fragments arranged by size:
2028 1341 761
BclI T'GATC_A
Cuts at: 1650 1650
Size: 4130
BglII A'GATC_T
Cuts at: 1645 3682 1645
Size: 2037 2093
BlpI GC'TnA_GC
Cuts at: 1242 1242
Size: 4130
Bme1580I G_kGCm'C
Cuts at: 241 450 1755 1848 2904 241
Size: 209 1305 93 1056 1467
Fragments arranged by size:
1467 1305 1056 209 93
BmrI ACTGGGnnnn_n'
Cuts at: 666 818 1342 1423 1758 3902 666
Size: 152 524 81 335 2144 894
Fragments arranged by size:
2144 894 524 335 152 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2497 2497
Size: 4130
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 697 846 1306 2185 2677 2975 697
Size: 149 460 879 492 298 1852
Fragments arranged by size:
1852 879 492 460 298 149
BsaI GGTCTCn'nnnn_
Cuts at: 3834 3834
Size: 4130
BsaAI yAC'GTr
Cuts at: 1483 1483
Size: 4130
BsaBI GATnn'nnATC
Cuts at: 6 1644 3681 6
Size: 1638 2037 455
Fragments arranged by size:
2037 1638 455
BsaHI Gr'CG_yC
Cuts at: 736 771 1561 1809 2511 4007 736
Size: 35 790 248 702 1496 859
Fragments arranged by size:
1496 859 790 702 248 35
BsaWI w'CCGG_w
Cuts at: 218 1319 1840 2518 2792 2939 3350 218
Size: 1101 521 678 274 147 411 998
Fragments arranged by size:
1101 998 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 139 169 139
Size: 30 4100
BseYI C'CCAG_C
Cuts at: 129 1555 2313 2890 3386 129
Size: 1426 758 577 496 873
Fragments arranged by size:
1426 873 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 219 1672 1718 2926 104
Size: 115 1453 46 1208 1308
Fragments arranged by size:
1453 1308 1208 115 46
BsiHKAI G_wGCw'C
Cuts at: 599 1144 1922 2112 2904 599
Size: 545 778 190 792 1825
Fragments arranged by size:
1825 792 778 545 190
Bsp1286I G_dGCh'C
Cuts at: 241 450 599 906 1144 1755 1848 1922 2112
Size: 209 149 307 238 611 93 74 190
Cuts at: 2112 2174 2904 241
Size: 62 730 1467
Fragments arranged by size:
1467 730 611 307 238 209 190 149
93 74 62
BspEI T'CCGG_A
Cuts at: 3350 3350
Size: 4130
BspHI T'CATG_A
Cuts at: 3306 3306
Size: 4130
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1180 1394 1696 2077 1180
Size: 214 302 381 3233
Fragments arranged by size:
3233 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2422 2476 3960 4125 50
Size: 2372 54 1484 165 55
Fragments arranged by size:
2372 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 795 878 2042 795
Size: 83 1164 2883
Fragments arranged by size:
2883 1164 83
BsrFI r'CCGG_y
Cuts at: 2128 2309 2518 2128
Size: 181 209 3740
Fragments arranged by size:
3740 209 181
BssSI C'ACGA_G
Cuts at: 204 2401 2759 204
Size: 2197 358 1575
Fragments arranged by size:
2197 1575 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1507 1507
Size: 4130
BstBI TT'CG_AA
Cuts at: 2491 2491
Size: 4130
BstYI r'GATC_y
Cuts at: 1 257 951 963 1155 1622 1645 1980 2226
Size: 256 694 12 192 467 23 335 246
Cuts at: 2226 3238 3324 3336 3682 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 694 467 449 346 335 256 246
192 86 23 12 12
BtgI C'CryG_G
Cuts at: 185 185
Size: 4130
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2080 2261 2424 2080
Size: 181 163 3786
Fragments arranged by size:
3786 181 163
BtsI GCAGTG_nn'
Cuts at: 1080 1373 1080
Size: 293 3837
DraI TTT'AAA
Cuts at: 1129 1129
Size: 4130
DraIII CAC_nnn'GTG
Cuts at: 698 698
Size: 4130
DrdI GACnn_nn'nnGTC
Cuts at: 1836 2694 1836
Size: 858 3272
EaeI y'GGCC_r
Cuts at: 467 723 908 1715 1889 2280 2307 3652 467
Size: 256 185 807 174 391 27 1345 945
Fragments arranged by size:
1345 945 807 391 256 185 174 27
EagI C'GGCC_G
Cuts at: 1715 1715
Size: 4130
EarI CTCTTCn'nnn_
Cuts at: 509 641 2153 2363 509
Size: 132 1512 210 2276
Fragments arranged by size:
2276 1512 210 132
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2338 2648 2794 3940 2338
Size: 310 146 1146 2528
Fragments arranged by size:
2528 1146 310 146
EcoICRI GAG'CTC
Cuts at: 597 1142 597
Size: 545 3585
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 299 1136 1955 2387 3134 3402 299
Size: 837 819 432 747 268 1027
Fragments arranged by size:
1027 837 819 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 191 447 1269 191
Size: 256 822 3052
Fragments arranged by size:
3052 822 256
EcoRI G'AATT_C
Cuts at: 1134 1134
Size: 4130
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2149 2149
Size: 4130
FspI TGC'GCA
Cuts at: 1911 1911
Size: 4130
HaeII r_GCGC'y
Cuts at: 732 1564 1812 2834 3813 732
Size: 832 248 1022 979 1049
Fragments arranged by size:
1049 1022 979 832 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 418 450 888 920 943 975 1972 2002 2004
Size: 32 438 32 23 32 997 30 2
Cuts at: 2004 2034 418
Size: 30 2514
Fragments arranged by size:
2514 997 438 32 32 32 30 30
23 2
HincII GTy'rAC
Cuts at: 1026 1169 1026
Size: 143 3987
KasI G'GCGC_C
Cuts at: 1560 1808 1560
Size: 248 3882
KpnI G_GTAC'C
Cuts at: 1150 1150
Size: 4130
MluI A'CGCG_T
Cuts at: 3563 3563
Size: 4130
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 808 2801 2985 808
Size: 1993 184 1953
Fragments arranged by size:
1993 1953 184
MscI TGG'CCA
Cuts at: 469 725 910 1891 469
Size: 256 185 981 2708
Fragments arranged by size:
2708 981 256 185
MslI CAynn'nnrTG
Cuts at: 422 2119 2246 422
Size: 1697 127 2306
Fragments arranged by size:
2306 1697 127
MspA1I CmG'CkG
Cuts at: 161 187 254 812 894 1110 1241 1555 1915
Size: 26 67 558 82 216 131 314 360
Cuts at: 1915 2928 3173 161
Size: 1013 245 1118
Fragments arranged by size:
1118 1013 558 360 314 245 216 131
82 67 26
NaeI GCC'GGC
Cuts at: 2311 2311
Size: 4130
NarI GG'CG_CC
Cuts at: 1561 1809 1561
Size: 248 3882
NgoMIV G'CCGG_C
Cuts at: 2309 2309
Size: 4130
NspI r_CATG'y
Cuts at: 574 809 1183 1683 2590 574
Size: 235 374 500 907 2114
Fragments arranged by size:
2114 907 500 374 235
PciI A'CATG_T
Cuts at: 2586 2586
Size: 4130
PflMI CCAn_nnn'nTGG
Cuts at: 698 732 698
Size: 34 4096
PmeI GTTT'AAAC
Cuts at: 1129 1129
Size: 4130
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3269 3301 3269
Size: 32 4098
PpuMI rG'GwC_Cy
Cuts at: 191 191
Size: 4130
PspOMI G'GGCC_C
Cuts at: 446 446
Size: 4130
PstI C_TGCA'G
Cuts at: 1075 1177 1862 1075
Size: 102 685 3343
Fragments arranged by size:
3343 685 102
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4130
PvuII CAG'CTG
Cuts at: 254 894 1555 1915 254
Size: 640 661 360 2469
Fragments arranged by size:
2469 661 640 360
RsrII CG'GwC_CG
Cuts at: 2325 2325
Size: 4130
SacI G_AGCT'C
Cuts at: 599 1144 599
Size: 545 3585
SacII CC_GC'GG
Cuts at: 188 188
Size: 4130
SalI G'TCGA_C
Cuts at: 1167 1167
Size: 4130
SanDI GG'GwC_CC
Cuts at: 191 191
Size: 4130
SapI GCTCTTCn'nnn_
Cuts at: 2153 2363 2153
Size: 210 3920
SbfI CC_TGCA'GG
Cuts at: 1177 1177
Size: 4130
ScaI AGT'ACT
Cuts at: 3647 3647
Size: 4130
SfcI C'TryA_G
Cuts at: 33 1071 1173 1858 2851 3042 33
Size: 1038 102 685 993 191 1121
Fragments arranged by size:
1121 1038 993 685 191 102
SfoI GGC'GCC
Cuts at: 1562 1810 1562
Size: 248 3882
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4130
SmaI CCC'GGG
Cuts at: 1152 1152
Size: 4130
SmlI C'TyrA_G
Cuts at: 409 676 861 1285 2200 2692 2954 409
Size: 267 185 424 915 492 262 1585
Fragments arranged by size:
1585 915 492 424 267 262 185
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4130
SphI G_CATG'C
Cuts at: 809 1183 1683 809
Size: 374 500 3256
Fragments arranged by size:
3256 500 374
StuI AGG'CCT
Cuts at: 1121 1121
Size: 4130
StyI C'CwwG_G
Cuts at: 1046 1264 1618 1046
Size: 218 354 3558
Fragments arranged by size:
3558 354 218
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 599 1713 1846 2305 2514 599
Size: 1114 133 459 209 2215
Fragments arranged by size:
2215 1114 459 209 133
TatI w'GTAC_w
Cuts at: 841 3645 841
Size: 2804 1326
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 139 171 3647 3679 139
Size: 32 3476 32 590
Fragments arranged by size:
3476 590 32 32
Tth111I GACn'n_nGTC
Cuts at: 1927 1927
Size: 4130
XbaI T'CTAG_A
Cuts at: 1161 1161
Size: 4130
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1625 1625
Size: 4130
XhoI C'TCGA_G
Cuts at: 409 409
Size: 4130
XmaI C'CCGG_G
Cuts at: 1150 1150
Size: 4130
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PciI PflMI PmeI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI BpmI Bpu10I BseRI BsgI BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI
CspCI EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NcoI NdeI NheI NotI NruI NsiI PacI PasI
PfoI PmlI PshAI PsiI PspXI PsrI SexAI SfiI
SgrAI SnaBI SrfI SspI SwaI XmnI ZraI