Restriction map for
pF1KB3408
(Circular) (Six-base) MAPSORT of: pF1KB3408.seq Check: 1170 from: 1 to: 6110
pF1KB3408 6110 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 180 180
Size: 6110
AccI GT'mk_AC
Cuts at: 986 1273 3148 986
Size: 287 1875 3948
Fragments arranged by size:
3948 1875 287
Acc65I G'GTAC_C
Cuts at: 1264 3126 1264
Size: 1862 4248
AclI AA'CG_TT
Cuts at: 5950 5950
Size: 6110
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 484 2260 2749 3935 4367 4720 5382 484
Size: 1776 489 1186 432 353 662 1212
Fragments arranged by size:
1776 1212 1186 662 489 432 353
AfeI AGC'GCT
Cuts at: 211 1678 211
Size: 1467 4643
AflIII A'CryG_T
Cuts at: 1466 3462 5262 5543 1466
Size: 1996 1800 281 2033
Fragments arranged by size:
2033 1996 1800 281
AleI CACnn'nnGTG
Cuts at: 2002 2281 2002
Size: 279 5831
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 319 532 1876 319
Size: 213 1344 4553
Fragments arranged by size:
4553 1344 213
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2167 2199 2167
Size: 32 6078
AlwNI CAG_nnn'CTG
Cuts at: 1491 1743 2600 2656 2871 3415 4853 1491
Size: 252 857 56 215 544 1438 2748
Fragments arranged by size:
2748 1438 857 544 252 215 56
ApaLI G'TGCA_C
Cuts at: 4948 4948
Size: 6110
ApoI r'AATT_y
Cuts at: 3114 5463 5719 3114
Size: 2349 256 3505
Fragments arranged by size:
3505 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6110
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6110
AvaI C'yCGr_G
Cuts at: 231 533 838 1030 1260 1279 1496 1571 1805
Size: 302 305 192 230 19 217 75 234
Cuts at: 1805 2095 2579 3130 231
Size: 290 484 551 3211
Fragments arranged by size:
3211 551 484 305 302 290 234 230
217 192 75 19
BamHI G'GATC_C
Cuts at: 524 3135 524
Size: 2611 3499
BanI G'GyrC_C
Cuts at: 739 1264 1513 2999 3126 3540 3788 3823 739
Size: 525 249 1486 127 414 248 35 3026
Fragments arranged by size:
3026 1486 525 414 249 248 127 35
BanII G_rGCy'C
Cuts at: 184 230 380 839 1234 1659 1768 2150 2272
Size: 46 150 459 395 425 109 382 122
Cuts at: 2272 2578 3124 4154 184
Size: 306 546 1030 2140
Fragments arranged by size:
2140 1030 546 459 425 395 382 306
150 122 109 46
BbeI G_GCGC'C
Cuts at: 1517 3003 3544 3792 1517
Size: 1486 541 248 3835
Fragments arranged by size:
3835 1486 541 248
BbsI GAAGACnn'nnnn_
Cuts at: 2051 2747 2051
Size: 696 5414
BbvCI CC'TCA_GC
Cuts at: 2089 2716 2089
Size: 627 5483
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1207 1241 2182 2216 1207
Size: 34 941 34 5101
Fragments arranged by size:
5101 941 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4008 5064 6110 4008
Size: 1056 1046 4008
Fragments arranged by size:
4008 1056 1046
BclI T'GATC_A
Cuts at: 1976 3630 1976
Size: 1654 4456
BglI GCCn_nnn'nGGC
Cuts at: 112 457 952 2293 2653 112
Size: 345 495 1341 360 3569
Fragments arranged by size:
3569 1341 495 360 345
BglII A'GATC_T
Cuts at: 3625 5662 3625
Size: 2037 4073
BlpI GC'TnA_GC
Cuts at: 3222 3222
Size: 6110
Bme1580I G_kGCm'C
Cuts at: 534 1075 1171 3735 3828 4952 534
Size: 541 96 2564 93 1124 1692
Fragments arranged by size:
2564 1692 1124 541 96 93
BmgBI CAC'GTC
Cuts at: 141 2049 141
Size: 1908 4202
BmrI ACTGGGnnnn_n'
Cuts at: 1078 3322 3403 3738 5882 1078
Size: 2244 81 335 2144 1306
Fragments arranged by size:
2244 2144 1306 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2605 4477 2605
Size: 1872 4238
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1582 1713 3004 1582
Size: 131 1291 4688
Fragments arranged by size:
4688 1291 131
Bpu10I CC'TnA_GC
Cuts at: 178 1767 2089 2271 2716 178
Size: 1589 322 182 445 3572
Fragments arranged by size:
3572 1589 445 322 182
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3043 3286 4165 4879 5177 3043
Size: 243 879 714 298 3976
Fragments arranged by size:
3976 879 714 298 243
BsaI GGTCTCn'nnnn_
Cuts at: 349 1120 1568 5814 349
Size: 771 448 4246 645
Fragments arranged by size:
4246 771 645 448
BsaAI yAC'GTr
Cuts at: 1271 2637 3463 1271
Size: 1366 826 3918
Fragments arranged by size:
3918 1366 826
BsaBI GATnn'nnATC
Cuts at: 6 2257 3624 5661 6
Size: 2251 1367 2037 455
Fragments arranged by size:
2251 2037 1367 455
BsaHI Gr'CG_yC
Cuts at: 847 1514 2684 3000 3541 3789 4491 5987 847
Size: 667 1170 316 541 248 702 1496 970
Fragments arranged by size:
1496 1170 970 702 667 541 316 248
BsaWI w'CCGG_w
Cuts at: 812 1577 3299 3820 4498 4909 5056 5330 812
Size: 765 1722 521 678 411 147 274 1592
Fragments arranged by size:
1722 1592 765 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 949 979 2314 2344 2386 2416 2621 2651 2851
Size: 30 1335 30 42 30 205 30 200
Cuts at: 2851 2881 949
Size: 30 4178
Fragments arranged by size:
4178 1335 205 200 42 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 358 367 2365 2394 358
Size: 9 1998 29 4074
Fragments arranged by size:
4074 1998 29 9
BseYI C'CCAG_C
Cuts at: 801 3535 4293 4958 5366 801
Size: 2734 758 665 408 1545
Fragments arranged by size:
2734 1545 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 538 775 1122 1584 1882 2497 2920 538
Size: 237 347 462 298 615 423 3728
Fragments arranged by size:
3728 615 462 423 347 298 237
BsiEI CG_ry'CG
Cuts at: 104 134 365 2282 3652 3698 4928 104
Size: 30 231 1917 1370 46 1230 1286
Fragments arranged by size:
1917 1370 1286 1230 231 46 30
BsiHKAI G_wGCw'C
Cuts at: 230 2737 2819 3020 3124 3902 4092 4952 230
Size: 2507 82 201 104 778 190 860 1388
Fragments arranged by size:
2507 1388 860 778 201 190 104 82
BsmBI CGTCTCn'nnnn_
Cuts at: 1864 2859 1864
Size: 995 5115
Bsp1286I G_dGCh'C
Cuts at: 184 230 380 534 839 1075 1171 1234 1659
Size: 46 150 154 305 236 96 63 425
Cuts at: 1659 1768 2150 2272 2578 2737 2819 3020 3124
Size: 109 382 122 306 159 82 201 104
Cuts at: 3124 3735 3828 3902 4092 4154 4952 184
Size: 611 93 74 190 62 798 1342
Fragments arranged by size:
1342 798 611 425 382 306 305 236
201 190 159 154 150 122 109 104
96 93 82 74 63 62 46
BspEI T'CCGG_A
Cuts at: 812 812
Size: 6110
BspHI T'CATG_A
Cuts at: 1973 4542 1973
Size: 2569 3541
BspMI ACCTGCnnnn'nnnn_
Cuts at: 180 1490 2186 2523 3160 3374 3676 4057 180
Size: 1310 696 337 637 214 302 381 2233
Fragments arranged by size:
2233 1310 696 637 381 337 302 214
BsrBI CCG'CTC
Cuts at: 50 500 843 2751 2996 4402 4456 5940 6105
Size: 450 343 1908 245 1406 54 1484 165
Cuts at: 6105 50
Size: 55
Fragments arranged by size:
1908 1484 1406 450 343 245 165 55
54
BsrDI GCAATG_nn'
Cuts at: 2844 4022 2844
Size: 1178 4932
BsrFI r'CCGG_y
Cuts at: 196 784 2209 2758 4108 4289 196
Size: 588 1425 549 1350 181 2017
Fragments arranged by size:
2017 1425 1350 588 549 181
BssHII G'CGCG_C
Cuts at: 200 200
Size: 6110
BssSI C'ACGA_G
Cuts at: 400 1246 2818 2953 4381 5089 400
Size: 846 1572 135 1428 708 1421
Fragments arranged by size:
1572 1428 1421 846 708 135
BstAPI GCAn_nnn'nTGC
Cuts at: 1542 3487 1542
Size: 1945 4165
BstBI TT'CG_AA
Cuts at: 4471 4471
Size: 6110
BstEII G'GTnAC_C
Cuts at: 192 2982 192
Size: 2790 3320
BstXI CCAn_nnnn'nTGG
Cuts at: 2526 2802 2928 2526
Size: 276 126 5708
Fragments arranged by size:
5708 276 126
BstYI r'GATC_y
Cuts at: 1 524 809 1739 1751 3135 3602 3625 3960
Size: 523 285 930 12 1384 467 23 335
Cuts at: 3960 4206 4512 4524 4610 5662 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
1384 1052 930 523 467 449 335 306
285 246 86 23 12 12
BstZ17I GTA'TAC
Cuts at: 1274 1274
Size: 6110
Bsu36I CC'TnA_GG
Cuts at: 1922 1922
Size: 6110
BtgI C'CryG_G
Cuts at: 107 776 957 1004 1121 1427 2003 2264 2282
Size: 669 181 47 117 306 576 261 18
Cuts at: 2282 2519 2801 2927 107
Size: 237 282 126 3290
Fragments arranged by size:
3290 669 576 306 282 261 237 181
126 117 47 18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2228 2244 4060 4241 4404 2228
Size: 16 1816 181 163 3934
Fragments arranged by size:
3934 1816 181 163 16
BtsI GCAGTG_nn'
Cuts at: 575 1997 2198 3353 575
Size: 1422 201 1155 3332
Fragments arranged by size:
3332 1422 1155 201
DraI TTT'AAA
Cuts at: 3109 3109
Size: 6110
DraIII CAC_nnn'GTG
Cuts at: 2669 2669
Size: 6110
DrdI GACnn_nn'nnGTC
Cuts at: 3816 5160 3816
Size: 1344 4766
EaeI y'GGCC_r
Cuts at: 131 404 773 779 914 936 1022 1320 1626
Size: 273 369 6 135 22 86 298 306
Cuts at: 1626 1799 2006 2279 2285 2645 2918 3695 3869
Size: 173 207 273 6 360 273 777 174
Cuts at: 3869 4260 4287 5632 131
Size: 391 27 1345 609
Fragments arranged by size:
1345 777 609 391 369 360 306 298
273 273 273 207 174 173 135 86
27 22 6 6
EagI C'GGCC_G
Cuts at: 131 2279 3695 131
Size: 2148 1416 2546
Fragments arranged by size:
2546 2148 1416
EarI CTCTTCn'nnn_
Cuts at: 2611 4133 4343 2611
Size: 1522 210 4378
Fragments arranged by size:
4378 1522 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 586 4318 5060 5206 5920 586
Size: 3732 742 146 714 776
Fragments arranged by size:
3732 776 742 714 146
EcoICRI GAG'CTC
Cuts at: 228 3122 228
Size: 2894 3216
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 484 1582 1713 2260 2749 3004 3935 4367 4720
Size: 1098 131 547 489 255 931 432 353
Cuts at: 4720 5382 484
Size: 662 1212
Fragments arranged by size:
1212 1098 931 662 547 489 432 353
255 131
EcoO109I rG'GnC_Cy
Cuts at: 282 459 1035 2692 3054 3249 282
Size: 177 576 1657 362 195 3143
Fragments arranged by size:
3143 1657 576 362 195 177
EcoRI G'AATT_C
Cuts at: 3114 3114
Size: 6110
EcoRV GAT'ATC
Cuts at: 3026 3026
Size: 6110
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4129 4129
Size: 6110
FspI TGC'GCA
Cuts at: 295 3891 295
Size: 3596 2514
FspAI rTGC'GCAy
Cuts at: 295 295
Size: 6110
HaeII r_GCGC'y
Cuts at: 213 983 1517 1680 2109 3003 3544 3792 5022
Size: 770 534 163 429 894 541 248 1230
Cuts at: 5022 5793 213
Size: 771 530
Fragments arranged by size:
1230 894 771 770 541 534 530 429
248 163
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 598 630 1216 1248 1567 1599 1750 1782 2684
Size: 32 586 32 319 32 151 32 902
Cuts at: 2684 2716 2851 2883 3952 3982 3984 4014 598
Size: 32 135 32 1069 30 2 30 2694
Fragments arranged by size:
2694 1069 902 586 319 151 135 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 3149 3149
Size: 6110
KasI G'GCGC_C
Cuts at: 1513 2999 3540 3788 1513
Size: 1486 541 248 3835
Fragments arranged by size:
3835 1486 541 248
KpnI G_GTAC'C
Cuts at: 1268 3130 1268
Size: 1862 4248
MluI A'CGCG_T
Cuts at: 5543 5543
Size: 6110
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1181 4869 5053 1181
Size: 3688 184 2238
Fragments arranged by size:
3688 2238 184
MscI TGG'CCA
Cuts at: 406 781 1628 1801 2008 2287 2647 2920 3871
Size: 375 847 173 207 279 360 273 951
Cuts at: 3871 406
Size: 2645
Fragments arranged by size:
2645 951 847 375 360 279 273 207
173
MslI CAynn'nnrTG
Cuts at: 901 1591 1966 2002 2203 2263 2281 2800 2830
Size: 690 375 36 201 60 18 519 30
Cuts at: 2830 2926 4099 4226 901
Size: 96 1173 127 2785
Fragments arranged by size:
2785 1173 690 519 375 201 127 96
60 36 30 18
MspA1I CmG'CkG
Cuts at: 852 885 1539 2129 2481 2715 3221 3535 3895
Size: 33 654 590 352 234 506 314 360
Cuts at: 3895 4679 4924 852
Size: 784 245 2038
Fragments arranged by size:
2038 784 654 590 506 360 352 314
245 234 33
NaeI GCC'GGC
Cuts at: 2760 4291 2760
Size: 1531 4579
NarI GG'CG_CC
Cuts at: 1514 3000 3541 3789 1514
Size: 1486 541 248 3835
Fragments arranged by size:
3835 1486 541 248
NcoI C'CATG_G
Cuts at: 107 2264 2801 2927 107
Size: 2157 537 126 3290
Fragments arranged by size:
3290 2157 537 126
NgoMIV G'CCGG_C
Cuts at: 2758 4289 2758
Size: 1531 4579
NspI r_CATG'y
Cuts at: 729 1061 1905 3084 3163 3663 5266 729
Size: 332 844 1179 79 500 1603 1573
Fragments arranged by size:
1603 1573 1179 844 500 332 79
PasI CC'CwG_GG
Cuts at: 547 1131 547
Size: 584 5526
PciI A'CATG_T
Cuts at: 5262 5262
Size: 6110
PflMI CCAn_nnn'nTGG
Cuts at: 2669 3009 2669
Size: 340 5770
PfoI T'CCnGG_A
Cuts at: 1754 3070 1754
Size: 1316 4794
PmeI GTTT'AAAC
Cuts at: 3109 3109
Size: 6110
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4556 4588 4556
Size: 32 6078
PpuMI rG'GwC_Cy
Cuts at: 282 2692 282
Size: 2410 3700
PstI C_TGCA'G
Cuts at: 857 1487 1852 2183 3157 3842 857
Size: 630 365 331 974 685 3125
Fragments arranged by size:
3125 974 685 630 365 331
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6110
PvuII CAG'CTG
Cuts at: 885 1539 2481 2715 3535 3895 885
Size: 654 942 234 820 360 3100
Fragments arranged by size:
3100 942 820 654 360 234
RsrII CG'GwC_CG
Cuts at: 504 4305 504
Size: 3801 2309
SacI G_AGCT'C
Cuts at: 230 3124 230
Size: 2894 3216
SalI G'TCGA_C
Cuts at: 3147 3147
Size: 6110
SapI GCTCTTCn'nnn_
Cuts at: 2611 4133 4343 2611
Size: 1522 210 4378
Fragments arranged by size:
4378 1522 210
SbfI CC_TGCA'GG
Cuts at: 1487 3157 1487
Size: 1670 4440
ScaI AGT'ACT
Cuts at: 5627 5627
Size: 6110
SexAI A'CCwGG_T
Cuts at: 2495 2665 2495
Size: 170 5940
SfcI C'TryA_G
Cuts at: 33 853 1483 1680 1848 2179 2367 3153 3838
Size: 820 630 197 168 331 188 786 685
Cuts at: 3838 4806 4997 33
Size: 968 191 1146
Fragments arranged by size:
1146 968 820 786 685 630 331 197
191 188 168
SfoI GGC'GCC
Cuts at: 1515 3001 3542 3790 1515
Size: 1486 541 248 3835
Fragments arranged by size:
3835 1486 541 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6110
SmaI CCC'GGG
Cuts at: 535 1498 1807 3132 535
Size: 963 309 1325 3513
Fragments arranged by size:
3513 1325 963 309
SmlI C'TyrA_G
Cuts at: 3058 3265 4180 4894 5156 3058
Size: 207 915 714 262 4012
Fragments arranged by size:
4012 915 714 262 207
SnaBI TAC'GTA
Cuts at: 2637 2637
Size: 6110
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6110
SphI G_CATG'C
Cuts at: 1905 3163 3663 1905
Size: 1258 500 4352
Fragments arranged by size:
4352 1258 500
StuI AGG'CCT
Cuts at: 2062 2113 2642 2062
Size: 51 529 5530
Fragments arranged by size:
5530 529 51
StyI C'CwwG_G
Cuts at: 107 1397 1819 2264 2801 2927 3244 3598 107
Size: 1290 422 445 537 126 317 354 2619
Fragments arranged by size:
2619 1290 537 445 422 354 317 126
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 757 2478 2997 3693 3826 4285 4494 757
Size: 1721 519 696 133 459 209 2373
Fragments arranged by size:
2373 1721 696 519 459 209 133
TatI w'GTAC_w
Cuts at: 1595 5625 1595
Size: 4030 2080
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 949 981 5627 5659 949
Size: 32 4646 32 1400
Fragments arranged by size:
4646 1400 32 32
Tth111I GACn'n_nGTC
Cuts at: 541 3907 541
Size: 3366 2744
XbaI T'CTAG_A
Cuts at: 3141 3141
Size: 6110
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 414 627 3605 414
Size: 213 2978 2919
Fragments arranged by size:
2978 2919 213
XmaI C'CCGG_G
Cuts at: 533 1496 1805 3130 533
Size: 963 309 1325 3513
Fragments arranged by size:
3513 1325 963 309
XmnI GAAnn'nnTTC
Cuts at: 1468 1552 2383 1468
Size: 84 831 5195
Fragments arranged by size:
5195 831 84
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AleI
AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI
FspAI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SnaBI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AgeI AhdI ApaI AscI AvrII BaeI
BfrBI BmtI BsiWI BsmI BsrGI ClaI CspCI EcoNI
FseI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PmlI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SfiI SgrAI SrfI SspI SwaI XhoI
ZraI