Restriction map for
pF1KB3401
(Circular) (Six-base) MAPSORT of: pF1KB3401.seq Check: 5011 from: 1 to: 3860
pF1KB3401 3860 bp
With 182 enzymes: SgfI *
April 24, 2009 13:01 ..
AccI GT'mk_AC
Cuts at: 898 898
Size: 3860
Acc65I G'GTAC_C
Cuts at: 876 876
Size: 3860
AclI AA'CG_TT
Cuts at: 3700 3700
Size: 3860
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 709 1685 2117 2864 3132 709
Size: 976 432 747 268 1437
Fragments arranged by size:
1437 976 747 432 268
AflIII A'CryG_T
Cuts at: 654 1212 2316 3293 654
Size: 558 1104 977 1221
Fragments arranged by size:
1221 1104 977 558
AgeI A'CCGG_T
Cuts at: 2248 2248
Size: 3860
AlwNI CAG_nnn'CTG
Cuts at: 136 1165 2732 136
Size: 1029 1567 1264
Fragments arranged by size:
1567 1264 1029
ApaI G_GGCC'C
Cuts at: 511 511
Size: 3860
ApaLI G'TGCA_C
Cuts at: 2630 2630
Size: 3860
ApoI r'AATT_y
Cuts at: 864 3213 3469 864
Size: 2349 256 1255
Fragments arranged by size:
2349 1255 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3860
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3860
AvaI C'yCGr_G
Cuts at: 880 880
Size: 3860
AvrII C'CTAG_G
Cuts at: 613 613
Size: 3860
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 476 509 476
Size: 33 3827
BamHI G'GATC_C
Cuts at: 885 885
Size: 3860
BanI G'GyrC_C
Cuts at: 876 1290 1538 1573 876
Size: 414 248 35 3163
Fragments arranged by size:
3163 414 248 35
BanII G_rGCy'C
Cuts at: 511 874 1904 511
Size: 363 1030 2467
Fragments arranged by size:
2467 1030 363
BbeI G_GCGC'C
Cuts at: 1294 1542 1294
Size: 248 3612
BciVI GTATCCnnnnn_n'
Cuts at: 1758 2519 3860 1758
Size: 761 1341 1758
Fragments arranged by size:
1758 1341 761
BclI T'GATC_A
Cuts at: 1380 1380
Size: 3860
BglI GCCn_nnn'nGGC
Cuts at: 506 506
Size: 3860
BglII A'GATC_T
Cuts at: 1375 3412 1375
Size: 2037 1823
BlpI GC'TnA_GC
Cuts at: 972 972
Size: 3860
Bme1580I G_kGCm'C
Cuts at: 511 1485 1578 2634 511
Size: 974 93 1056 1737
Fragments arranged by size:
1737 1056 974 93
BmrI ACTGGGnnnn_n'
Cuts at: 1072 1153 1488 3632 1072
Size: 81 335 2144 1300
Fragments arranged by size:
2144 1300 335 81
BmtI G_CTAG'C
Cuts at: 524 524
Size: 3860
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2227 2227
Size: 3860
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 395 395
Size: 3860
Bpu10I CC'TnA_GC
Cuts at: 767 767
Size: 3860
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1036 1915 2407 2705 1036
Size: 879 492 298 2191
Fragments arranged by size:
2191 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3564 3564
Size: 3860
BsaAI yAC'GTr
Cuts at: 655 1213 655
Size: 558 3302
BsaBI GATnn'nnATC
Cuts at: 6 419 1374 3411 6
Size: 413 955 2037 455
Fragments arranged by size:
2037 955 455 413
BsaHI Gr'CG_yC
Cuts at: 1291 1539 2241 3737 1291
Size: 248 702 1496 1414
Fragments arranged by size:
1496 1414 702 248
BsaWI w'CCGG_w
Cuts at: 1049 1570 2248 2522 2669 3080 1049
Size: 521 678 274 147 411 1829
Fragments arranged by size:
1829 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 129 1285 2043 2620 3116 129
Size: 1156 758 577 496 873
Fragments arranged by size:
1156 873 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1402 1448 2656 104
Size: 1298 46 1208 1308
Fragments arranged by size:
1308 1298 1208 46
BsiHKAI G_wGCw'C
Cuts at: 874 1652 1842 2634 874
Size: 778 190 792 2100
Fragments arranged by size:
2100 792 778 190
BsmI GAATG_Cn'
Cuts at: 348 348
Size: 3860
BsmBI CGTCTCn'nnnn_
Cuts at: 792 792
Size: 3860
Bsp1286I G_dGCh'C
Cuts at: 511 874 1485 1578 1652 1842 1904 2634 511
Size: 363 611 93 74 190 62 730 1737
Fragments arranged by size:
1737 730 611 363 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3080 3080
Size: 3860
BspHI T'CATG_A
Cuts at: 3036 3036
Size: 3860
BspMI ACCTGCnnnn'nnnn_
Cuts at: 910 1124 1426 1807 910
Size: 214 302 381 2963
Fragments arranged by size:
2963 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2152 2206 3690 3855 50
Size: 2102 54 1484 165 55
Fragments arranged by size:
2102 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1772 1772
Size: 3860
BsrFI r'CCGG_y
Cuts at: 1858 2039 2248 1858
Size: 181 209 3470
Fragments arranged by size:
3470 209 181
BssSI C'ACGA_G
Cuts at: 2131 2489 2131
Size: 358 3502
BstAPI GCAn_nnn'nTGC
Cuts at: 733 1237 733
Size: 504 3356
BstBI TT'CG_AA
Cuts at: 2221 2221
Size: 3860
BstYI r'GATC_y
Cuts at: 1 885 1352 1375 1710 1956 2968 3054 3066
Size: 884 467 23 335 246 1012 86 12
Cuts at: 3066 3412 1
Size: 346 449
Fragments arranged by size:
1012 884 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 315 315
Size: 3860
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1810 1991 2154 1810
Size: 181 163 3516
Fragments arranged by size:
3516 181 163
BtsI GCAGTG_nn'
Cuts at: 1103 1103
Size: 3860
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 195 230 195
Size: 35 3825
DraI TTT'AAA
Cuts at: 859 859
Size: 3860
DrdI GACnn_nn'nnGTC
Cuts at: 1566 2424 1566
Size: 858 3002
EaeI y'GGCC_r
Cuts at: 203 318 746 1445 1619 2010 2037 3382 203
Size: 115 428 699 174 391 27 1345 681
Fragments arranged by size:
1345 699 681 428 391 174 115 27
EagI C'GGCC_G
Cuts at: 1445 1445
Size: 3860
EarI CTCTTCn'nnn_
Cuts at: 713 790 1883 2093 713
Size: 77 1093 210 2480
Fragments arranged by size:
2480 1093 210 77
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 316 556 2068 2378 2524 3670 316
Size: 240 1512 310 146 1146 506
Fragments arranged by size:
1512 1146 506 310 240 146
EcoICRI GAG'CTC
Cuts at: 872 872
Size: 3860
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 395 709 1685 2117 2864 3132 395
Size: 314 976 432 747 268 1123
Fragments arranged by size:
1123 976 747 432 314 268
EcoO109I rG'GnC_Cy
Cuts at: 508 999 508
Size: 491 3369
EcoRI G'AATT_C
Cuts at: 864 864
Size: 3860
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1879 1879
Size: 3860
FspI TGC'GCA
Cuts at: 1641 1641
Size: 3860
HaeII r_GCGC'y
Cuts at: 780 1294 1542 2564 3543 780
Size: 514 248 1022 979 1097
Fragments arranged by size:
1097 1022 979 514 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 624 656 1702 1732 1734 1764 624
Size: 32 1046 30 2 30 2720
Fragments arranged by size:
2720 1046 32 30 30 2
HincII GTy'rAC
Cuts at: 899 899
Size: 3860
KasI G'GCGC_C
Cuts at: 1290 1538 1290
Size: 248 3612
KpnI G_GTAC'C
Cuts at: 880 880
Size: 3860
MluI A'CGCG_T
Cuts at: 3293 3293
Size: 3860
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2531 2715 2531
Size: 184 3676
MscI TGG'CCA
Cuts at: 205 748 1621 205
Size: 543 873 2444
Fragments arranged by size:
2444 873 543
MslI CAynn'nnrTG
Cuts at: 157 320 1849 1976 157
Size: 163 1529 127 2041
Fragments arranged by size:
2041 1529 163 127
MspA1I CmG'CkG
Cuts at: 971 1285 1645 2658 2903 971
Size: 314 360 1013 245 1928
Fragments arranged by size:
1928 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2041 2041
Size: 3860
NarI GG'CG_CC
Cuts at: 1291 1539 1291
Size: 248 3612
NcoI C'CATG_G
Cuts at: 315 315
Size: 3860
NgoMIV G'CCGG_C
Cuts at: 2039 2039
Size: 3860
NheI G'CTAG_C
Cuts at: 520 520
Size: 3860
NspI r_CATG'y
Cuts at: 913 1413 2320 913
Size: 500 907 2453
Fragments arranged by size:
2453 907 500
PciI A'CATG_T
Cuts at: 2316 2316
Size: 3860
PflMI CCAn_nnn'nTGG
Cuts at: 136 136
Size: 3860
PfoI T'CCnGG_A
Cuts at: 471 471
Size: 3860
PmeI GTTT'AAAC
Cuts at: 859 859
Size: 3860
PmlI CAC'GTG
Cuts at: 655 655
Size: 3860
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2999 3031 2999
Size: 32 3828
PspOMI G'GGCC_C
Cuts at: 507 507
Size: 3860
PstI C_TGCA'G
Cuts at: 665 729 907 1592 665
Size: 64 178 685 2933
Fragments arranged by size:
2933 685 178 64
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3860
PvuII CAG'CTG
Cuts at: 1285 1645 1285
Size: 360 3500
RsrII CG'GwC_CG
Cuts at: 2055 2055
Size: 3860
SacI G_AGCT'C
Cuts at: 874 874
Size: 3860
SalI G'TCGA_C
Cuts at: 897 897
Size: 3860
SapI GCTCTTCn'nnn_
Cuts at: 1883 2093 1883
Size: 210 3650
SbfI CC_TGCA'GG
Cuts at: 907 907
Size: 3860
ScaI AGT'ACT
Cuts at: 3377 3377
Size: 3860
SfcI C'TryA_G
Cuts at: 33 488 661 725 903 1588 2581 2772 33
Size: 455 173 64 178 685 993 191 1121
Fragments arranged by size:
1121 993 685 455 191 178 173 64
SfoI GGC'GCC
Cuts at: 1292 1540 1292
Size: 248 3612
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3860
SmaI CCC'GGG
Cuts at: 882 882
Size: 3860
SmlI C'TyrA_G
Cuts at: 1015 1930 2422 2684 1015
Size: 915 492 262 2191
Fragments arranged by size:
2191 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3860
SphI G_CATG'C
Cuts at: 913 1413 913
Size: 500 3360
StyI C'CwwG_G
Cuts at: 315 364 613 994 1348 315
Size: 49 249 381 354 2827
Fragments arranged by size:
2827 381 354 249 49
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 220 642 1443 1576 2035 2244 220
Size: 422 801 133 459 209 1836
Fragments arranged by size:
1836 801 459 422 209 133
TatI w'GTAC_w
Cuts at: 3375 3375
Size: 3860
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3377 3409 3377
Size: 32 3828
Tth111I GACn'n_nGTC
Cuts at: 1657 1657
Size: 3860
XbaI T'CTAG_A
Cuts at: 891 891
Size: 3860
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1355 1355
Size: 3860
XmaI C'CCGG_G
Cuts at: 880 880
Size: 3860
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI
BanI BanII BbeI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseYI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NheI NspI PciI PflMI PfoI PmeI
PmlI PpiI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI BbsI BbvCI BcgI BfrBI BmgBI BsaXI BseRI
BsgI BsiWI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I
ClaI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NotI NruI NsiI PacI PasI PpuMI
PshAI PsiI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI