Restriction map for
pF1KB3398
(Circular) (Six-base) MAPSORT of: pF1KB3398.seq Check: 1659 from: 1 to: 3566
pF1KB3398 3566 bp
With 182 enzymes: SgfI *
April 24, 2009 13:01 ..
AccI GT'mk_AC
Cuts at: 604 604
Size: 3566
Acc65I G'GTAC_C
Cuts at: 582 582
Size: 3566
AclI AA'CG_TT
Cuts at: 3406 3406
Size: 3566
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 383 1391 1823 2570 2838 383
Size: 1008 432 747 268 1111
Fragments arranged by size:
1111 1008 747 432 268
AflII C'TTAA_G
Cuts at: 331 331
Size: 3566
AflIII A'CryG_T
Cuts at: 918 2022 2999 918
Size: 1104 977 1485
Fragments arranged by size:
1485 1104 977
AgeI A'CCGG_T
Cuts at: 1954 1954
Size: 3566
AhdI GACnn_n'nnGTC
Cuts at: 169 169
Size: 3566
AlwNI CAG_nnn'CTG
Cuts at: 397 547 871 2438 397
Size: 150 324 1567 1525
Fragments arranged by size:
1567 1525 324 150
ApaLI G'TGCA_C
Cuts at: 2336 2336
Size: 3566
ApoI r'AATT_y
Cuts at: 317 326 442 570 2919 3175 317
Size: 9 116 128 2349 256 708
Fragments arranged by size:
2349 708 256 128 116 9
AseI AT'TA_AT
Cuts at: 20 20
Size: 3566
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3566
AvaI C'yCGr_G
Cuts at: 127 586 127
Size: 459 3107
BamHI G'GATC_C
Cuts at: 591 591
Size: 3566
BanI G'GyrC_C
Cuts at: 582 996 1244 1279 582
Size: 414 248 35 2869
Fragments arranged by size:
2869 414 248 35
BanII G_rGCy'C
Cuts at: 580 1610 580
Size: 1030 2536
BbeI G_GCGC'C
Cuts at: 1000 1248 1000
Size: 248 3318
BciVI GTATCCnnnnn_n'
Cuts at: 1464 2225 3566 1464
Size: 761 1341 1464
Fragments arranged by size:
1464 1341 761
BclI T'GATC_A
Cuts at: 1086 1086
Size: 3566
BfrBI ATG'CAT
Cuts at: 120 120
Size: 3566
BglII A'GATC_T
Cuts at: 366 1081 3118 366
Size: 715 2037 814
Fragments arranged by size:
2037 814 715
BlpI GC'TnA_GC
Cuts at: 678 678
Size: 3566
Bme1580I G_kGCm'C
Cuts at: 1191 1284 2340 1191
Size: 93 1056 2417
Fragments arranged by size:
2417 1056 93
BmgBI CAC'GTC
Cuts at: 204 204
Size: 3566
BmrI ACTGGGnnnn_n'
Cuts at: 138 778 859 1194 3338 138
Size: 640 81 335 2144 366
Fragments arranged by size:
2144 640 366 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1933 1933
Size: 3566
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 433 742 1621 2113 2411 433
Size: 309 879 492 298 1588
Fragments arranged by size:
1588 879 492 309 298
BsaI GGTCTCn'nnnn_
Cuts at: 3270 3270
Size: 3566
BsaAI yAC'GTr
Cuts at: 919 919
Size: 3566
BsaBI GATnn'nnATC
Cuts at: 6 1080 3117 6
Size: 1074 2037 455
Fragments arranged by size:
2037 1074 455
BsaHI Gr'CG_yC
Cuts at: 997 1245 1947 3443 997
Size: 248 702 1496 1120
Fragments arranged by size:
1496 1120 702 248
BsaWI w'CCGG_w
Cuts at: 755 1276 1954 2228 2375 2786 755
Size: 521 678 274 147 411 1535
Fragments arranged by size:
1535 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 991 1749 2326 2822 991
Size: 758 577 496 1735
Fragments arranged by size:
1735 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1108 1154 2362 104
Size: 1004 46 1208 1308
Fragments arranged by size:
1308 1208 1004 46
BsiHKAI G_wGCw'C
Cuts at: 580 1358 1548 2340 580
Size: 778 190 792 1806
Fragments arranged by size:
1806 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 580 1191 1284 1358 1548 1610 2340 580
Size: 611 93 74 190 62 730 1806
Fragments arranged by size:
1806 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2786 2786
Size: 3566
BspHI T'CATG_A
Cuts at: 2742 2742
Size: 3566
BspMI ACCTGCnnnn'nnnn_
Cuts at: 616 830 1132 1513 616
Size: 214 302 381 2669
Fragments arranged by size:
2669 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1858 1912 3396 3561 50
Size: 1808 54 1484 165 55
Fragments arranged by size:
1808 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1478 1478
Size: 3566
BsrFI r'CCGG_y
Cuts at: 1564 1745 1954 1564
Size: 181 209 3176
Fragments arranged by size:
3176 209 181
BssSI C'ACGA_G
Cuts at: 1837 2195 1837
Size: 358 3208
BstAPI GCAn_nnn'nTGC
Cuts at: 943 943
Size: 3566
BstBI TT'CG_AA
Cuts at: 402 1927 402
Size: 1525 2041
BstYI r'GATC_y
Cuts at: 1 366 591 1058 1081 1416 1662 2674 2760
Size: 365 225 467 23 335 246 1012 86
Cuts at: 2760 2772 3118 1
Size: 12 346 449
Fragments arranged by size:
1012 467 449 365 346 335 246 225
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3566
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1516 1697 1860 1516
Size: 181 163 3222
Fragments arranged by size:
3222 181 163
BtsI GCAGTG_nn'
Cuts at: 809 809
Size: 3566
DraI TTT'AAA
Cuts at: 565 565
Size: 3566
DraIII CAC_nnn'GTG
Cuts at: 283 283
Size: 3566
DrdI GACnn_nn'nnGTC
Cuts at: 1272 2130 1272
Size: 858 2708
EaeI y'GGCC_r
Cuts at: 227 1151 1325 1716 1743 3088 227
Size: 924 174 391 27 1345 705
Fragments arranged by size:
1345 924 705 391 174 27
EagI C'GGCC_G
Cuts at: 1151 1151
Size: 3566
EarI CTCTTCn'nnn_
Cuts at: 1589 1799 1589
Size: 210 3356
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1774 2084 2230 3376 1774
Size: 310 146 1146 1964
Fragments arranged by size:
1964 1146 310 146
EcoICRI GAG'CTC
Cuts at: 578 578
Size: 3566
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 383 1391 1823 2570 2838 383
Size: 1008 432 747 268 1111
Fragments arranged by size:
1111 1008 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 135 237 705 135
Size: 102 468 2996
Fragments arranged by size:
2996 468 102
EcoRI G'AATT_C
Cuts at: 326 570 326
Size: 244 3322
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1585 1585
Size: 3566
FspI TGC'GCA
Cuts at: 1347 1347
Size: 3566
HaeII r_GCGC'y
Cuts at: 1000 1248 2270 3249 1000
Size: 248 1022 979 1317
Fragments arranged by size:
1317 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 155 187 1408 1438 1440 1470 155
Size: 32 1221 30 2 30 2251
Fragments arranged by size:
2251 1221 32 30 30 2
HincII GTy'rAC
Cuts at: 191 605 191
Size: 414 3152
KasI G'GCGC_C
Cuts at: 996 1244 996
Size: 248 3318
KpnI G_GTAC'C
Cuts at: 586 586
Size: 3566
MfeI C'AATT_G
Cuts at: 519 519
Size: 3566
MluI A'CGCG_T
Cuts at: 2999 2999
Size: 3566
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2237 2421 2237
Size: 184 3382
MscI TGG'CCA
Cuts at: 229 1327 229
Size: 1098 2468
MslI CAynn'nnrTG
Cuts at: 1555 1682 1555
Size: 127 3439
MspA1I CmG'CkG
Cuts at: 677 991 1351 2364 2609 677
Size: 314 360 1013 245 1634
Fragments arranged by size:
1634 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1747 1747
Size: 3566
NarI GG'CG_CC
Cuts at: 997 1245 997
Size: 248 3318
NcoI C'CATG_G
Cuts at: 107 107
Size: 3566
NgoMIV G'CCGG_C
Cuts at: 1745 1745
Size: 3566
NsiI A_TGCA'T
Cuts at: 122 122
Size: 3566
NspI r_CATG'y
Cuts at: 120 124 619 1119 2026 120
Size: 4 495 500 907 1660
Fragments arranged by size:
1660 907 500 495 4
PacI TTA_AT'TAA
Cuts at: 383 383
Size: 3566
PciI A'CATG_T
Cuts at: 2022 2022
Size: 3566
PmeI GTTT'AAAC
Cuts at: 565 565
Size: 3566
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2705 2737 2705
Size: 32 3534
PsiI TTA'TAA
Cuts at: 494 494
Size: 3566
PstI C_TGCA'G
Cuts at: 613 1298 613
Size: 685 2881
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3566
PvuII CAG'CTG
Cuts at: 991 1351 991
Size: 360 3206
RsrII CG'GwC_CG
Cuts at: 1761 1761
Size: 3566
SacI G_AGCT'C
Cuts at: 580 580
Size: 3566
SalI G'TCGA_C
Cuts at: 603 603
Size: 3566
SapI GCTCTTCn'nnn_
Cuts at: 1589 1799 1589
Size: 210 3356
SbfI CC_TGCA'GG
Cuts at: 613 613
Size: 3566
ScaI AGT'ACT
Cuts at: 3083 3083
Size: 3566
SfcI C'TryA_G
Cuts at: 33 539 609 1294 2287 2478 33
Size: 506 70 685 993 191 1121
Fragments arranged by size:
1121 993 685 506 191 70
SfoI GGC'GCC
Cuts at: 998 1246 998
Size: 248 3318
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3566
SmaI CCC'GGG
Cuts at: 129 588 129
Size: 459 3107
SmlI C'TyrA_G
Cuts at: 331 412 721 1636 2128 2390 331
Size: 81 309 915 492 262 1507
Fragments arranged by size:
1507 915 492 309 262 81
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3566
SphI G_CATG'C
Cuts at: 120 124 619 1119 120
Size: 4 495 500 2567
Fragments arranged by size:
2567 500 495 4
StyI C'CwwG_G
Cuts at: 107 700 1054 107
Size: 593 354 2619
Fragments arranged by size:
2619 593 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1149 1282 1741 1950 1149
Size: 133 459 209 2765
Fragments arranged by size:
2765 459 209 133
TatI w'GTAC_w
Cuts at: 3081 3081
Size: 3566
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3083 3115 3083
Size: 32 3534
Tth111I GACn'n_nGTC
Cuts at: 1363 1363
Size: 3566
XbaI T'CTAG_A
Cuts at: 597 597
Size: 3566
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1061 1061
Size: 3566
XmaI C'CCGG_G
Cuts at: 127 586 127
Size: 459 3107
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BfrBI BglII BlpI Bme1580I
BmgBI BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI
PacI PciI PmeI PpiI PsiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AleI AlfI AloI ApaI AscI
AvrII BaeI BbsI BbvCI BcgI BglI BmtI BpmI
Bpu10I BsaXI BseRI BsgI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI EcoNI
EcoRV FseI FspAI HindIII HpaI NdeI NheI NotI
NruI PasI PflMI PfoI PmlI PpuMI PshAI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI