Restriction map for
pF1KB3393
(Circular) (Six-base) MAPSORT of: pF1KB3393.seq Check: 2806 from: 1 to: 4037
pF1KB3393 4037 bp
With 182 enzymes: SgfI *
April 24, 2009 13:01 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 675 675
Size: 4037
AccI GT'mk_AC
Cuts at: 1075 1075
Size: 4037
Acc65I G'GTAC_C
Cuts at: 236 1053 236
Size: 817 3220
AclI AA'CG_TT
Cuts at: 3877 3877
Size: 4037
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 304 997 1862 2294 3041 3309 304
Size: 693 865 432 747 268 1032
Fragments arranged by size:
1032 865 747 693 432 268
AflIII A'CryG_T
Cuts at: 1389 2493 3470 1389
Size: 1104 977 1956
Fragments arranged by size:
1956 1104 977
AgeI A'CCGG_T
Cuts at: 2425 2425
Size: 4037
AhdI GACnn_n'nnGTC
Cuts at: 465 896 465
Size: 431 3606
AlwNI CAG_nnn'CTG
Cuts at: 309 1342 2909 309
Size: 1033 1567 1437
Fragments arranged by size:
1567 1437 1033
ApaI G_GGCC'C
Cuts at: 357 401 357
Size: 44 3993
ApaLI G'TGCA_C
Cuts at: 487 550 2807 487
Size: 63 2257 1717
Fragments arranged by size:
2257 1717 63
ApoI r'AATT_y
Cuts at: 1041 3390 3646 1041
Size: 2349 256 1432
Fragments arranged by size:
2349 1432 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4037
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4037
AvaI C'yCGr_G
Cuts at: 1057 1057
Size: 4037
BamHI G'GATC_C
Cuts at: 1062 1062
Size: 4037
BanI G'GyrC_C
Cuts at: 236 319 1053 1467 1715 1750 236
Size: 83 734 414 248 35 2523
Fragments arranged by size:
2523 734 414 248 83 35
BanII G_rGCy'C
Cuts at: 182 357 401 681 1051 2081 182
Size: 175 44 280 370 1030 2138
Fragments arranged by size:
2138 1030 370 280 175 44
BbeI G_GCGC'C
Cuts at: 1471 1719 1471
Size: 248 3789
BbsI GAAGACnn'nnnn_
Cuts at: 369 369
Size: 4037
BciVI GTATCCnnnnn_n'
Cuts at: 1935 2696 4037 1935
Size: 761 1341 1935
Fragments arranged by size:
1935 1341 761
BclI T'GATC_A
Cuts at: 1557 1557
Size: 4037
BglI GCCn_nnn'nGGC
Cuts at: 725 725
Size: 4037
BglII A'GATC_T
Cuts at: 1552 3589 1552
Size: 2037 2000
BlpI GC'TnA_GC
Cuts at: 680 1149 680
Size: 469 3568
Bme1580I G_kGCm'C
Cuts at: 322 357 401 437 491 554 1662 1755 2811
Size: 35 44 36 54 63 1108 93 1056
Cuts at: 2811 322
Size: 1548
Fragments arranged by size:
1548 1108 1056 93 63 54 44 36
35
BmrI ACTGGGnnnn_n'
Cuts at: 621 898 1249 1330 1665 3809 621
Size: 277 351 81 335 2144 849
Fragments arranged by size:
2144 849 351 335 277 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2404 2404
Size: 4037
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 556 556
Size: 4037
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1213 2092 2584 2882 1213
Size: 879 492 298 2368
Fragments arranged by size:
2368 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3741 3741
Size: 4037
BsaAI yAC'GTr
Cuts at: 818 1390 818
Size: 572 3465
BsaBI GATnn'nnATC
Cuts at: 6 943 1551 3588 6
Size: 937 608 2037 455
Fragments arranged by size:
2037 937 608 455
BsaHI Gr'CG_yC
Cuts at: 1468 1716 2418 3914 1468
Size: 248 702 1496 1591
Fragments arranged by size:
1591 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1226 1747 2425 2699 2846 3257 1226
Size: 521 678 274 147 411 2006
Fragments arranged by size:
2006 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 142 172 544 574 142
Size: 30 372 30 3605
Fragments arranged by size:
3605 372 30 30
BseYI C'CCAG_C
Cuts at: 365 769 1462 2220 2797 3293 365
Size: 404 693 758 577 496 1109
Fragments arranged by size:
1109 758 693 577 496 404
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 585 585
Size: 4037
BsiEI CG_ry'CG
Cuts at: 104 1579 1625 2833 104
Size: 1475 46 1208 1308
Fragments arranged by size:
1475 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 491 554 681 1051 1829 2019 2811 491
Size: 63 127 370 778 190 792 1717
Fragments arranged by size:
1717 792 778 370 190 127 63
BsmBI CGTCTCn'nnnn_
Cuts at: 532 532
Size: 4037
Bsp1286I G_dGCh'C
Cuts at: 182 322 357 401 437 491 554 681 1051
Size: 140 35 44 36 54 63 127 370
Cuts at: 1051 1662 1755 1829 2019 2081 2811 182
Size: 611 93 74 190 62 730 1408
Fragments arranged by size:
1408 730 611 370 190 140 127 93
74 63 62 54 44 36 35
BspEI T'CCGG_A
Cuts at: 3257 3257
Size: 4037
BspHI T'CATG_A
Cuts at: 887 3213 887
Size: 2326 1711
BspMI ACCTGCnnnn'nnnn_
Cuts at: 422 675 767 1087 1301 1603 1984 422
Size: 253 92 320 214 302 381 2475
Fragments arranged by size:
2475 381 320 302 253 214 92
BsrBI CCG'CTC
Cuts at: 50 2329 2383 3867 4032 50
Size: 2279 54 1484 165 55
Fragments arranged by size:
2279 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 191 1949 191
Size: 1758 2279
BsrFI r'CCGG_y
Cuts at: 2035 2216 2425 2035
Size: 181 209 3647
Fragments arranged by size:
3647 209 181
BssSI C'ACGA_G
Cuts at: 2308 2666 2308
Size: 358 3679
BstAPI GCAn_nnn'nTGC
Cuts at: 309 1414 309
Size: 1105 2932
BstBI TT'CG_AA
Cuts at: 2398 2398
Size: 4037
BstXI CCAn_nnnn'nTGG
Cuts at: 744 744
Size: 4037
BstYI r'GATC_y
Cuts at: 1 788 1062 1529 1552 1887 2133 3145 3231
Size: 787 274 467 23 335 246 1012 86
Cuts at: 3231 3243 3589 1
Size: 12 346 449
Fragments arranged by size:
1012 787 467 449 346 335 274 246
86 23 12
BtgI C'CryG_G
Cuts at: 314 323 564 314
Size: 9 241 3787
Fragments arranged by size:
3787 241 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1987 2168 2331 1987
Size: 181 163 3693
Fragments arranged by size:
3693 181 163
BtsI GCAGTG_nn'
Cuts at: 238 901 1280 238
Size: 663 379 2995
Fragments arranged by size:
2995 663 379
DraI TTT'AAA
Cuts at: 1036 1036
Size: 4037
DrdI GACnn_nn'nnGTC
Cuts at: 1743 2601 1743
Size: 858 3179
EaeI y'GGCC_r
Cuts at: 326 447 1622 1796 2187 2214 3559 326
Size: 121 1175 174 391 27 1345 804
Fragments arranged by size:
1345 1175 804 391 174 121 27
EagI C'GGCC_G
Cuts at: 1622 1622
Size: 4037
EarI CTCTTCn'nnn_
Cuts at: 217 424 550 829 2060 2270 217
Size: 207 126 279 1231 210 1984
Fragments arranged by size:
1984 1231 279 210 207 126
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 247 2245 2555 2701 3847 247
Size: 1998 310 146 1146 437
Fragments arranged by size:
1998 1146 437 310 146
EcoICRI GAG'CTC
Cuts at: 679 1049 679
Size: 370 3667
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 304 556 997 1862 2294 3041 3309 304
Size: 252 441 865 432 747 268 1032
Fragments arranged by size:
1032 865 747 441 432 268 252
EcoO109I rG'GnC_Cy
Cuts at: 345 353 397 398 756 866 1176 345
Size: 8 44 1 358 110 310 3206
Fragments arranged by size:
3206 358 310 110 44 8 1
EcoRI G'AATT_C
Cuts at: 1041 1041
Size: 4037
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 228 828 2056 228
Size: 600 1228 2209
Fragments arranged by size:
2209 1228 600
FspI TGC'GCA
Cuts at: 583 1006 1818 583
Size: 423 812 2802
Fragments arranged by size:
2802 812 423
HaeII r_GCGC'y
Cuts at: 531 700 1471 1719 2741 3720 531
Size: 169 771 248 1022 979 848
Fragments arranged by size:
1022 979 848 771 248 169
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 451 483 850 882 914 1879 1909 1911 1941
Size: 32 367 32 32 965 30 2 30
Cuts at: 1941 451
Size: 2547
Fragments arranged by size:
2547 965 367 32 32 32 30 30
2
HincII GTy'rAC
Cuts at: 1076 1076
Size: 4037
KasI G'GCGC_C
Cuts at: 1467 1715 1467
Size: 248 3789
KpnI G_GTAC'C
Cuts at: 240 1057 240
Size: 817 3220
MluI A'CGCG_T
Cuts at: 3470 3470
Size: 4037
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2708 2892 2708
Size: 184 3853
MscI TGG'CCA
Cuts at: 449 1798 449
Size: 1349 2688
MslI CAynn'nnrTG
Cuts at: 455 742 937 2026 2153 455
Size: 287 195 1089 127 2339
Fragments arranged by size:
2339 1089 287 195 127
MspA1I CmG'CkG
Cuts at: 365 769 1148 1462 1822 2835 3080 365
Size: 404 379 314 360 1013 245 1322
Fragments arranged by size:
1322 1013 404 379 360 314 245
NaeI GCC'GGC
Cuts at: 2218 2218
Size: 4037
NarI GG'CG_CC
Cuts at: 1468 1716 1468
Size: 248 3789
NcoI C'CATG_G
Cuts at: 564 564
Size: 4037
NgoMIV G'CCGG_C
Cuts at: 2216 2216
Size: 4037
NspI r_CATG'y
Cuts at: 1011 1090 1590 2497 1011
Size: 79 500 907 2551
Fragments arranged by size:
2551 907 500 79
PciI A'CATG_T
Cuts at: 2493 2493
Size: 4037
PfoI T'CCnGG_A
Cuts at: 341 868 986 341
Size: 527 118 3392
Fragments arranged by size:
3392 527 118
PmeI GTTT'AAAC
Cuts at: 1036 1036
Size: 4037
PmlI CAC'GTG
Cuts at: 818 818
Size: 4037
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3176 3208 3176
Size: 32 4005
PpuMI rG'GwC_Cy
Cuts at: 345 756 866 345
Size: 411 110 3516
Fragments arranged by size:
3516 411 110
PspOMI G'GGCC_C
Cuts at: 353 397 353
Size: 44 3993
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 589 621 589
Size: 32 4005
PstI C_TGCA'G
Cuts at: 305 1084 1769 305
Size: 779 685 2573
Fragments arranged by size:
2573 779 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4037
PvuII CAG'CTG
Cuts at: 365 1462 1822 365
Size: 1097 360 2580
Fragments arranged by size:
2580 1097 360
RsrII CG'GwC_CG
Cuts at: 2232 2232
Size: 4037
SacI G_AGCT'C
Cuts at: 681 1051 681
Size: 370 3667
SalI G'TCGA_C
Cuts at: 1074 1074
Size: 4037
SapI GCTCTTCn'nnn_
Cuts at: 424 550 829 2060 2270 424
Size: 126 279 1231 210 2191
Fragments arranged by size:
2191 1231 279 210 126
SbfI CC_TGCA'GG
Cuts at: 1084 1084
Size: 4037
ScaI AGT'ACT
Cuts at: 3554 3554
Size: 4037
SfcI C'TryA_G
Cuts at: 33 301 1080 1765 2758 2949 33
Size: 268 779 685 993 191 1121
Fragments arranged by size:
1121 993 779 685 268 191
SfoI GGC'GCC
Cuts at: 1469 1717 1469
Size: 248 3789
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4037
SmaI CCC'GGG
Cuts at: 1059 1059
Size: 4037
SmlI C'TyrA_G
Cuts at: 1192 2107 2599 2861 1192
Size: 915 492 262 2368
Fragments arranged by size:
2368 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4037
SphI G_CATG'C
Cuts at: 1011 1090 1590 1011
Size: 79 500 3458
Fragments arranged by size:
3458 500 79
StyI C'CwwG_G
Cuts at: 269 564 953 1171 1525 269
Size: 295 389 218 354 2781
Fragments arranged by size:
2781 389 354 295 218
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 357 949 1620 1753 2212 2421 357
Size: 592 671 133 459 209 1973
Fragments arranged by size:
1973 671 592 459 209 133
TatI w'GTAC_w
Cuts at: 335 3552 335
Size: 3217 820
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 142 174 323 355 544 576 3554 3586 142
Size: 32 149 32 189 32 2978 32 593
Fragments arranged by size:
2978 593 189 149 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1834 1834
Size: 4037
XbaI T'CTAG_A
Cuts at: 1068 1068
Size: 4037
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1532 1532
Size: 4037
XmaI C'CCGG_G
Cuts at: 1057 1057
Size: 4037
XmnI GAAnn'nnTTC
Cuts at: 211 811 982 211
Size: 600 171 3266
Fragments arranged by size:
3266 600 171
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PfoI PmeI PmlI PpiI
PpuMI PspOMI PsrI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AleI AlfI AloI AscI AvrII
BaeI BbvCI BcgI BfrBI BmgBI BmtI Bpu10I BseRI
BsiWI BsmI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PshAI PsiI PspXI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI ZraI