Restriction map for
pF1KB3388
(Circular) (Six-base) MAPSORT of: pF1KB3388.seq Check: 6640 from: 1 to: 3722
pF1KB3388 3722 bp
With 182 enzymes: SgfI *
April 24, 2009 13:01 ..
AccI GT'mk_AC
Cuts at: 760 760
Size: 3722
Acc65I G'GTAC_C
Cuts at: 738 738
Size: 3722
AclI AA'CG_TT
Cuts at: 3562 3562
Size: 3722
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 514 1547 1979 2726 2994 514
Size: 1033 432 747 268 1242
Fragments arranged by size:
1242 1033 747 432 268
AflIII A'CryG_T
Cuts at: 1074 2178 3155 1074
Size: 1104 977 1641
Fragments arranged by size:
1641 1104 977
AgeI A'CCGG_T
Cuts at: 2110 2110
Size: 3722
AhdI GACnn_n'nnGTC
Cuts at: 712 712
Size: 3722
AlwNI CAG_nnn'CTG
Cuts at: 126 189 1027 2594 126
Size: 63 838 1567 1254
Fragments arranged by size:
1567 1254 838 63
ApaI G_GGCC'C
Cuts at: 333 333
Size: 3722
ApaLI G'TGCA_C
Cuts at: 2492 2492
Size: 3722
ApoI r'AATT_y
Cuts at: 726 3075 3331 726
Size: 2349 256 1117
Fragments arranged by size:
2349 1117 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3722
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3722
AvaI C'yCGr_G
Cuts at: 308 742 308
Size: 434 3288
BamHI G'GATC_C
Cuts at: 747 747
Size: 3722
BanI G'GyrC_C
Cuts at: 524 738 1152 1400 1435 524
Size: 214 414 248 35 2811
Fragments arranged by size:
2811 414 248 214 35
BanII G_rGCy'C
Cuts at: 333 736 1766 333
Size: 403 1030 2289
Fragments arranged by size:
2289 1030 403
BbeI G_GCGC'C
Cuts at: 528 1156 1404 528
Size: 628 248 2846
Fragments arranged by size:
2846 628 248
BciVI GTATCCnnnnn_n'
Cuts at: 1620 2381 3722 1620
Size: 761 1341 1620
Fragments arranged by size:
1620 1341 761
BclI T'GATC_A
Cuts at: 1242 1242
Size: 3722
BglI GCCn_nnn'nGGC
Cuts at: 175 175
Size: 3722
BglII A'GATC_T
Cuts at: 1237 3274 1237
Size: 2037 1685
BlpI GC'TnA_GC
Cuts at: 834 834
Size: 3722
Bme1580I G_kGCm'C
Cuts at: 333 1347 1440 2496 333
Size: 1014 93 1056 1559
Fragments arranged by size:
1559 1056 1014 93
BmrI ACTGGGnnnn_n'
Cuts at: 934 1015 1350 3494 934
Size: 81 335 2144 1162
Fragments arranged by size:
2144 1162 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 166 2089 166
Size: 1923 1799
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 595 595
Size: 3722
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 397 463 679 898 1777 2269 2567 397
Size: 66 216 219 879 492 298 1552
Fragments arranged by size:
1552 879 492 298 219 216 66
BsaI GGTCTCn'nnnn_
Cuts at: 3426 3426
Size: 3722
BsaAI yAC'GTr
Cuts at: 1075 1075
Size: 3722
BsaBI GATnn'nnATC
Cuts at: 6 1236 3273 6
Size: 1230 2037 455
Fragments arranged by size:
2037 1230 455
BsaHI Gr'CG_yC
Cuts at: 525 1153 1401 2103 3599 525
Size: 628 248 702 1496 648
Fragments arranged by size:
1496 702 648 628 248
BsaWI w'CCGG_w
Cuts at: 611 911 1432 2110 2384 2531 2942 611
Size: 300 521 678 274 147 411 1391
Fragments arranged by size:
1391 678 521 411 300 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 592 592
Size: 3722
BseYI C'CCAG_C
Cuts at: 192 334 1147 1905 2482 2978 192
Size: 142 813 758 577 496 936
Fragments arranged by size:
936 813 758 577 496 142
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 139 139
Size: 3722
BsiEI CG_ry'CG
Cuts at: 104 1264 1310 2518 104
Size: 1160 46 1208 1308
Fragments arranged by size:
1308 1208 1160 46
BsiHKAI G_wGCw'C
Cuts at: 736 1514 1704 2496 736
Size: 778 190 792 1962
Fragments arranged by size:
1962 792 778 190
BsiWI C'GTAC_G
Cuts at: 213 213
Size: 3722
BsmBI CGTCTCn'nnnn_
Cuts at: 415 415
Size: 3722
Bsp1286I G_dGCh'C
Cuts at: 333 736 1347 1440 1514 1704 1766 2496 333
Size: 403 611 93 74 190 62 730 1559
Fragments arranged by size:
1559 730 611 403 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2942 2942
Size: 3722
BspHI T'CATG_A
Cuts at: 629 2898 629
Size: 2269 1453
BspMI ACCTGCnnnn'nnnn_
Cuts at: 111 772 986 1288 1669 111
Size: 661 214 302 381 2164
Fragments arranged by size:
2164 661 381 302 214
BsrBI CCG'CTC
Cuts at: 50 400 2014 2068 3552 3717 50
Size: 350 1614 54 1484 165 55
Fragments arranged by size:
1614 1484 350 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1634 1634
Size: 3722
BsrFI r'CCGG_y
Cuts at: 1720 1901 2110 1720
Size: 181 209 3332
Fragments arranged by size:
3332 209 181
BssSI C'ACGA_G
Cuts at: 535 1993 2351 535
Size: 1458 358 1906
Fragments arranged by size:
1906 1458 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1099 1099
Size: 3722
BstBI TT'CG_AA
Cuts at: 2083 2083
Size: 3722
BstYI r'GATC_y
Cuts at: 1 602 747 1214 1237 1572 1818 2830 2916
Size: 601 145 467 23 335 246 1012 86
Cuts at: 2916 2928 3274 1
Size: 12 346 449
Fragments arranged by size:
1012 601 467 449 346 335 246 145
86 23 12
BtgI C'CryG_G
Cuts at: 107 702 107
Size: 595 3127
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1672 1853 2016 1672
Size: 181 163 3378
Fragments arranged by size:
3378 181 163
BtsI GCAGTG_nn'
Cuts at: 566 965 566
Size: 399 3323
DraI TTT'AAA
Cuts at: 721 721
Size: 3722
DrdI GACnn_nn'nnGTC
Cuts at: 1428 2286 1428
Size: 858 2864
EaeI y'GGCC_r
Cuts at: 167 1307 1481 1872 1899 3244 167
Size: 1140 174 391 27 1345 645
Fragments arranged by size:
1345 1140 645 391 174 27
EagI C'GGCC_G
Cuts at: 1307 1307
Size: 3722
EarI CTCTTCn'nnn_
Cuts at: 577 1745 1955 577
Size: 1168 210 2344
Fragments arranged by size:
2344 1168 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 127 366 1930 2240 2386 3532 127
Size: 239 1564 310 146 1146 317
Fragments arranged by size:
1564 1146 317 310 239 146
EcoICRI GAG'CTC
Cuts at: 734 734
Size: 3722
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 514 595 1547 1979 2726 2994 514
Size: 81 952 432 747 268 1242
Fragments arranged by size:
1242 952 747 432 268 81
EcoNI CCTnn'n_nnAGG
Cuts at: 308 308
Size: 3722
EcoO109I rG'GnC_Cy
Cuts at: 123 313 330 690 861 123
Size: 190 17 360 171 2984
Fragments arranged by size:
2984 360 190 171 17
EcoRI G'AATT_C
Cuts at: 726 726
Size: 3722
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1741 1741
Size: 3722
FspI TGC'GCA
Cuts at: 1503 1503
Size: 3722
HaeII r_GCGC'y
Cuts at: 528 1156 1404 2426 3405 528
Size: 628 248 1022 979 845
Fragments arranged by size:
1022 979 845 628 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 698 730 1564 1594 1596 1626 698
Size: 32 834 30 2 30 2794
Fragments arranged by size:
2794 834 32 30 30 2
HincII GTy'rAC
Cuts at: 761 761
Size: 3722
KasI G'GCGC_C
Cuts at: 524 1152 1400 524
Size: 628 248 2846
Fragments arranged by size:
2846 628 248
KpnI G_GTAC'C
Cuts at: 742 742
Size: 3722
MluI A'CGCG_T
Cuts at: 3155 3155
Size: 3722
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2393 2577 2393
Size: 184 3538
MscI TGG'CCA
Cuts at: 1483 1483
Size: 3722
MslI CAynn'nnrTG
Cuts at: 1711 1838 1711
Size: 127 3595
MspA1I CmG'CkG
Cuts at: 833 1147 1507 2520 2765 833
Size: 314 360 1013 245 1790
Fragments arranged by size:
1790 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1903 1903
Size: 3722
NarI GG'CG_CC
Cuts at: 525 1153 1401 525
Size: 628 248 2846
Fragments arranged by size:
2846 628 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 3722
NgoMIV G'CCGG_C
Cuts at: 1901 1901
Size: 3722
NspI r_CATG'y
Cuts at: 775 1275 2182 775
Size: 500 907 2315
Fragments arranged by size:
2315 907 500
PciI A'CATG_T
Cuts at: 2178 2178
Size: 3722
PflMI CCAn_nnn'nTGG
Cuts at: 153 153
Size: 3722
PmeI GTTT'AAAC
Cuts at: 721 721
Size: 3722
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2861 2893 2861
Size: 32 3690
PpuMI rG'GwC_Cy
Cuts at: 123 690 123
Size: 567 3155
PspOMI G'GGCC_C
Cuts at: 329 329
Size: 3722
PstI C_TGCA'G
Cuts at: 769 1454 769
Size: 685 3037
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3722
PvuII CAG'CTG
Cuts at: 1147 1507 1147
Size: 360 3362
RsrII CG'GwC_CG
Cuts at: 353 1917 353
Size: 1564 2158
SacI G_AGCT'C
Cuts at: 736 736
Size: 3722
SalI G'TCGA_C
Cuts at: 759 759
Size: 3722
SapI GCTCTTCn'nnn_
Cuts at: 1745 1955 1745
Size: 210 3512
SbfI CC_TGCA'GG
Cuts at: 769 769
Size: 3722
ScaI AGT'ACT
Cuts at: 3239 3239
Size: 3722
SfcI C'TryA_G
Cuts at: 33 249 765 1450 2443 2634 33
Size: 216 516 685 993 191 1121
Fragments arranged by size:
1121 993 685 516 216 191
SfoI GGC'GCC
Cuts at: 526 1154 1402 526
Size: 628 248 2846
Fragments arranged by size:
2846 628 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3722
SmaI CCC'GGG
Cuts at: 744 744
Size: 3722
SmlI C'TyrA_G
Cuts at: 412 478 694 877 1792 2284 2546 412
Size: 66 216 183 915 492 262 1588
Fragments arranged by size:
1588 915 492 262 216 183 66
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3722
SphI G_CATG'C
Cuts at: 775 1275 775
Size: 500 3222
StyI C'CwwG_G
Cuts at: 107 856 1210 107
Size: 749 354 2619
Fragments arranged by size:
2619 749 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1305 1438 1897 2106 1305
Size: 133 459 209 2921
Fragments arranged by size:
2921 459 209 133
TatI w'GTAC_w
Cuts at: 3237 3237
Size: 3722
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3239 3271 3239
Size: 32 3690
Tth111I GACn'n_nGTC
Cuts at: 1519 1519
Size: 3722
XbaI T'CTAG_A
Cuts at: 753 753
Size: 3722
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1217 1217
Size: 3722
XmaI C'CCGG_G
Cuts at: 742 742
Size: 3722
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PflMI PmeI PpiI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AloI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BmgBI BmtI
Bpu10I BsaXI BsmI BsrGI BssHII BstEII BstXI BstZ17I
Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PfoI PmlI PshAI PsiI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI